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... the Tg(hsp70otpa;hsp70YFP) line otpa, full-length coding sequence from pCS2-otpa (Ryu et al., 2007) was inserted into a dual heat shock promoter expression plasmid (Dirk Meyer, Innsbruck, unpublished) to obtain hsp70otpa;hsp70YFP. A single G0 founder (allele designation m1072) was used to create a s ...
... the Tg(hsp70otpa;hsp70YFP) line otpa, full-length coding sequence from pCS2-otpa (Ryu et al., 2007) was inserted into a dual heat shock promoter expression plasmid (Dirk Meyer, Innsbruck, unpublished) to obtain hsp70otpa;hsp70YFP. A single G0 founder (allele designation m1072) was used to create a s ...
Spherical particles of halophilic archaea correlate with exposure to
... laboratory, we exposed rod-shaped cells of Halobacterium species to reduced external water activity (aw). Gradual formation of spheres of about 0.4 lm diameter occurred in 4 M NaCl buffer of aw 0.75, but exposure to buffered 4 M LiCl (aw 0.73) split cells into spheres within seconds, with concom ...
... laboratory, we exposed rod-shaped cells of Halobacterium species to reduced external water activity (aw). Gradual formation of spheres of about 0.4 lm diameter occurred in 4 M NaCl buffer of aw 0.75, but exposure to buffered 4 M LiCl (aw 0.73) split cells into spheres within seconds, with concom ...
Role of Integration Host Factor in the Transcriptional Activation of
... In the Caulobacter crescentus predivisional cell, class III and IV flagellar genes, encoding the extracytoplasmic components of the flagellum, are transcribed in the nascent swarmer compartment. This asymmetric expression pattern is attributable to the compartmentalized activity of the 54-dependent ...
... In the Caulobacter crescentus predivisional cell, class III and IV flagellar genes, encoding the extracytoplasmic components of the flagellum, are transcribed in the nascent swarmer compartment. This asymmetric expression pattern is attributable to the compartmentalized activity of the 54-dependent ...
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... Whereas glycosphingolipids are indispensable for the development of complex multicellular organisms, phosphosphingolipids fulfill a vital function at a more fundamental level, namely, in the growth and survival of individual cells. This requirement for phosphosphingolipids appears to be a conserved ...
... Whereas glycosphingolipids are indispensable for the development of complex multicellular organisms, phosphosphingolipids fulfill a vital function at a more fundamental level, namely, in the growth and survival of individual cells. This requirement for phosphosphingolipids appears to be a conserved ...
Thesis titel 1.5 final - SciDok
... devastating and costly problems in medicine. In ageing population, they reduce quality of life for many at significant socioeconomic cost. All procedures that restore missing tissue in patients require some type of replacement structure for the area of defect or injury. These have traditionally been ...
... devastating and costly problems in medicine. In ageing population, they reduce quality of life for many at significant socioeconomic cost. All procedures that restore missing tissue in patients require some type of replacement structure for the area of defect or injury. These have traditionally been ...
Fluorescent properties of c-type cytochromes
... cytochromes in G. sulfurreducens can readily be monitored with fluorescence. The abundance of c-type cytochromes in G. sulfurreducens, coupled with the low level of fluorescence background from other cellular material at the selected excitation and emission wavelengths, makes it possible to detect r ...
... cytochromes in G. sulfurreducens can readily be monitored with fluorescence. The abundance of c-type cytochromes in G. sulfurreducens, coupled with the low level of fluorescence background from other cellular material at the selected excitation and emission wavelengths, makes it possible to detect r ...
Dynamics of the slowing segmentation clock reveal
... synchronizes neighboring cells and revealing that daughter cells oscillate synchronously after mitosis. The her1:her1-venus reporter is thus a powerful tool to explore how segmentation clock signal is translated to pattern each forming somite. In both fixed and live embryos, the cyclic gene expressi ...
... synchronizes neighboring cells and revealing that daughter cells oscillate synchronously after mitosis. The her1:her1-venus reporter is thus a powerful tool to explore how segmentation clock signal is translated to pattern each forming somite. In both fixed and live embryos, the cyclic gene expressi ...
Cleavage furrow formation and ingression during animal cytokinesis
... addition, this model cannot explain the furrowing phenotype observed in the zyg-9 and tac-1 mutants described above. Finally, if this hypothesis is correct, then one would expect that, if the centrosomes separate farther, multiple or ectopic furrows should form, as observed in microtubule depolymeri ...
... addition, this model cannot explain the furrowing phenotype observed in the zyg-9 and tac-1 mutants described above. Finally, if this hypothesis is correct, then one would expect that, if the centrosomes separate farther, multiple or ectopic furrows should form, as observed in microtubule depolymeri ...
physiology of ion transport across the tonoplast of higher plants
... salt-grown cells (80 mM NaCl) at the beginning of the stationary phase when compared with activities measured in the unadapted cell line at the same growth stage. In tobacco cell suspension cultures Reuveni et al (97) studied the hydrolytic and H+-transport activities of the V-ATPase in unadapted vs ...
... salt-grown cells (80 mM NaCl) at the beginning of the stationary phase when compared with activities measured in the unadapted cell line at the same growth stage. In tobacco cell suspension cultures Reuveni et al (97) studied the hydrolytic and H+-transport activities of the V-ATPase in unadapted vs ...
Disorders Presenting in Skin and Mucous Membranes - McGraw-Hill
... proteinase 3) process hCAP18 into the effector molecule LL-37, which plays an important role in cutaneous host defense because of its pronounced antibacterial,9,10 antifungal,11 and antiviral12,13 activities. LL-37 further contributes to innate immunity by attracting mast cells and neutrophils via f ...
... proteinase 3) process hCAP18 into the effector molecule LL-37, which plays an important role in cutaneous host defense because of its pronounced antibacterial,9,10 antifungal,11 and antiviral12,13 activities. LL-37 further contributes to innate immunity by attracting mast cells and neutrophils via f ...
Dual mode of paraxial mesoderm formation during chick gastrulation
... mesoderm. In double- (Fig. 1d) or single-graft (Fig. 1 i and l) experiments analyzed after 24 h, cells from the 90% level of the streak mostly gave rise to cells in medial somites from the anterior level of the somitic region to the tail bud (SI Table 1; Fig. 1 e, g, and j). In contrast, grafts of m ...
... mesoderm. In double- (Fig. 1d) or single-graft (Fig. 1 i and l) experiments analyzed after 24 h, cells from the 90% level of the streak mostly gave rise to cells in medial somites from the anterior level of the somitic region to the tail bud (SI Table 1; Fig. 1 e, g, and j). In contrast, grafts of m ...
An Empirical Study of End-User Behaviour in Spreadsheet Error
... correction yield might have been if individuals had been placed in groups of three, all combinations of three students were created and the number of separate errors corrected identified. The average performance across all combinations was calculated to represent the likely performance on average of ...
... correction yield might have been if individuals had been placed in groups of three, all combinations of three students were created and the number of separate errors corrected identified. The average performance across all combinations was calculated to represent the likely performance on average of ...
pep Curriculum Modules for Teachers of High School Biology & Chemistry
... Description of the module The ability to detect drugs in hair is becoming a highly accepted method for drug testing in sports, in the workplace and in hospitals. In this module, the distribution of drugs throughout the bloodstream and their accumulation in a specific target (i.e., hair) is described ...
... Description of the module The ability to detect drugs in hair is becoming a highly accepted method for drug testing in sports, in the workplace and in hospitals. In this module, the distribution of drugs throughout the bloodstream and their accumulation in a specific target (i.e., hair) is described ...
Stat3 and MMP7 Contribute to Pancreatic Ductal Adenocarcinoma
... The investigators firstly examine the expression of activated Stat3 in the caerulin-induced acute pancreatitis in wild-type and KRASG12D mice model, which showed caerulin treatment would induce the expression of activated Stat3 in the pancreas and persistent Stat3 activation in Kras-driven ductal me ...
... The investigators firstly examine the expression of activated Stat3 in the caerulin-induced acute pancreatitis in wild-type and KRASG12D mice model, which showed caerulin treatment would induce the expression of activated Stat3 in the pancreas and persistent Stat3 activation in Kras-driven ductal me ...
Zebrafish germ cell migration - Development
... migration is defective in hands off mutants, where the intermediate mesoderm of the presumptive gonadal region is mispatterned. Our results indicate that zebrafish PGCs are guided by attraction towards two signaling centers, one of which may represent the somatic tissues of the gonad. ...
... migration is defective in hands off mutants, where the intermediate mesoderm of the presumptive gonadal region is mispatterned. Our results indicate that zebrafish PGCs are guided by attraction towards two signaling centers, one of which may represent the somatic tissues of the gonad. ...
Patent ductus arteriosus in mice with smooth muscle
... most common human congenital heart defects. PDA patients are at increased risk of pulmonary and cardiac problems such as pulmonary hemorrhage, congestive heart failure, chronic lung disease, sepsis and necrotizing enterocolitis (Clyman, 2006; Forsey et al., 2009; Schneider and Moore, 2006). Mice wit ...
... most common human congenital heart defects. PDA patients are at increased risk of pulmonary and cardiac problems such as pulmonary hemorrhage, congestive heart failure, chronic lung disease, sepsis and necrotizing enterocolitis (Clyman, 2006; Forsey et al., 2009; Schneider and Moore, 2006). Mice wit ...
Name: Date: ______ Period: ______ 2015-16
... Rats and humans belong to Kingdom Animalia, Phylum Chorodata, Subphylum Vertebrata, and Class Mammalia. The two primary mammalian characteristics that distinguish them from other vertebrates such as fish, amphibians, birds, and reptiles, is that their skin is covered with hair or fur and they have m ...
... Rats and humans belong to Kingdom Animalia, Phylum Chorodata, Subphylum Vertebrata, and Class Mammalia. The two primary mammalian characteristics that distinguish them from other vertebrates such as fish, amphibians, birds, and reptiles, is that their skin is covered with hair or fur and they have m ...
Phagosome maturation in unicellular eukaryote Paramecium: the
... pathway to ingest bacteria and other food particles. Digestion process starts up upon fusion of phagosomes (i.e. digestive vacuoles at stage II; DV-II) with the primary lysosomes (Allen and Fok 2000) on which we focused our analysis looking for molecular machinery involved in phagolysosome formation ...
... pathway to ingest bacteria and other food particles. Digestion process starts up upon fusion of phagosomes (i.e. digestive vacuoles at stage II; DV-II) with the primary lysosomes (Allen and Fok 2000) on which we focused our analysis looking for molecular machinery involved in phagolysosome formation ...
A Role for the Basal Forebrain Cholinergic System in Estrogen
... Estrogen transiently disinhibits hippocampal CA1 pyramidal cells in adult female rats and prolongs the decay time of IPSCs in these cells. Estrogen-induced changes in synaptic inhibition are likely to be causally related to subsequent enhancements in excitatory synaptic function in CA1 pyramidal cel ...
... Estrogen transiently disinhibits hippocampal CA1 pyramidal cells in adult female rats and prolongs the decay time of IPSCs in these cells. Estrogen-induced changes in synaptic inhibition are likely to be causally related to subsequent enhancements in excitatory synaptic function in CA1 pyramidal cel ...
ATP1A1-Mediated Src Signaling Inhibits Coronavirus Entry into Host
... containing 10% FBS was added, and cells were incubated for 7 h at 37°C in 5% CO2. At time points indicated in the text or figure legends, the medium was replaced by warm medium containing 50 nM ouabain. At 7 hpi cells were lysed, and luciferase expression was analyzed as described above. Effect of o ...
... containing 10% FBS was added, and cells were incubated for 7 h at 37°C in 5% CO2. At time points indicated in the text or figure legends, the medium was replaced by warm medium containing 50 nM ouabain. At 7 hpi cells were lysed, and luciferase expression was analyzed as described above. Effect of o ...
Specific vasopressin binding to rat adrenal glomerulosa cells
... (Gallo-Payet et al., 1985) indicated that vasopressin increases the concentration of InsP, InsP2 and InsP3 in adrenal cells in primary culture. We therefore decided to compare the dose-dependency for vasopressin binding to glomerulosa cells with that of the vasopressin-induced increase in InsP, InsP ...
... (Gallo-Payet et al., 1985) indicated that vasopressin increases the concentration of InsP, InsP2 and InsP3 in adrenal cells in primary culture. We therefore decided to compare the dose-dependency for vasopressin binding to glomerulosa cells with that of the vasopressin-induced increase in InsP, InsP ...
High density lipoprotein-associated lysosphingolipids reduce E
... induction decreased thereafter and reached basal levels by 24–48 h (not shown). Neither HDL nor SPC and LSF affected total E-selectin levels in unstimulated cells. However, preincubation of HUVECs for 15 min with HDL (1 g/L), SPC (10 lmol/L), and LSF (20 lmol/L) prior to cytokine stimulation for 6 h ...
... induction decreased thereafter and reached basal levels by 24–48 h (not shown). Neither HDL nor SPC and LSF affected total E-selectin levels in unstimulated cells. However, preincubation of HUVECs for 15 min with HDL (1 g/L), SPC (10 lmol/L), and LSF (20 lmol/L) prior to cytokine stimulation for 6 h ...
Journal of Bacteriology
... in press), except that only one precursor was observed, which had the same chromatographic behavior as lysine-containing UDP-MurNAcpentapeptide. In the presence of prasinomycin, we observed a strong decrease on the basis spot activity (without antibiotic, 6,050 counts/min; with prasinomycin, 1,670 c ...
... in press), except that only one precursor was observed, which had the same chromatographic behavior as lysine-containing UDP-MurNAcpentapeptide. In the presence of prasinomycin, we observed a strong decrease on the basis spot activity (without antibiotic, 6,050 counts/min; with prasinomycin, 1,670 c ...