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Organization and translation of mRNA in sympathetic axons
Organization and translation of mRNA in sympathetic axons

... with a BamHI site, and subcloned into pGEM-T easy. The 723 bp fragment containing the whole coding region and a part of the 3′ UTR of chick ADF (provided by J. R. Bamburg, nucleotides 88-811, GenBank J02912) was subcloned into pGEM-3Z at the BamHI and EcoRI sites. Each construct was linearized with ...
Autophagy and cell death in model organisms
Autophagy and cell death in model organisms

... organisms has implicated autophagy in the cell death process. Cell death is of paramount importance both for the development and also during the adult life of animals, by forming and deleting structures, controlling cell numbers, eliminating abnormal or damaged cells and contributing in many patholo ...
PDF + SI - Development - The Company of Biologists
PDF + SI - Development - The Company of Biologists

... Endosperm breakdown requires the endosperm-specific basic Helix Loop Helix transcription factor ZHOUPI. However, to date the mechanism underlying the Arabidopsis endosperm breakdown process has not been elucidated. Here we provide evidence that ZHOUPI does not induce the developmental Programmed Cel ...
Lung cysts in Birt-Hogg-Dubé syndrome: Histopathological
Lung cysts in Birt-Hogg-Dubé syndrome: Histopathological

... pathological data suggest that the families with germline mutation of BHD gene allele are potentially affected by typical symptom of the skin in their youth, and that the risk of renal cell carcinomas increases as they become middle-aged. Contrary to the neoplastic processes in the skin and the kidn ...
Crayfish Observation and Dissection Purpose: In this lab, you will
Crayfish Observation and Dissection Purpose: In this lab, you will

... abdomen. The cephalothorax consists of the cephalic (or head) region and the thoracic region. The part of the exoskeleton that covers the cephalothorax is called the carapace. The abdomen is located behind the cephalothorax and consists of six clearly divided segments. The cephalothorax consists of ...
Autophagy and cell death in model organisms - IMBB
Autophagy and cell death in model organisms - IMBB

... organisms has implicated autophagy in the cell death process. Cell death is of paramount importance both for the development and also during the adult life of animals, by forming and deleting structures, controlling cell numbers, eliminating abnormal or damaged cells and contributing in many patholo ...
Debeer S et al, 2013
Debeer S et al, 2013

... been devoted to human skin, few data are available regarding the histology and the immunohistochemical profile of porcine skin [6, 8-16]. This is due in part to the fact that there are few antibodies which have been raised specifically to porcine skin cells. Because it is expected that in the future ...
PDF
PDF

... http://ccforum.com/content/18/6/657 ...
REGULATION OF CDC14: PATHWAYS AND CHECKPOINTS OF
REGULATION OF CDC14: PATHWAYS AND CHECKPOINTS OF

... maintaining the metaphase arrest in response to microtubule depolymerization by drug treatment (42). The majority of spindle checkpoint genes identified in this genetic screen regulate the ubiquitin ligase activity of APCCdc20 (43). On the other hand, Bub2 appears to be the only spindle checkpoint g ...
Actin branching in the initiation and maintenance of lamellipodia
Actin branching in the initiation and maintenance of lamellipodia

... actin filaments must be short and stiff to push (Pollard & Borisy, 2003). Since actin filaments in lamellipodia are densely packed, the resolution of their spatial organization requires electron tomography and in the first study using this approach (Urban et al., 2010) a dendritic array of short fil ...
1 Organization of Your Body
1 Organization of Your Body

... internal environment requires constant adjustments as conditions change inside and outside of cells. The endocrine system plays an important role in homeostasis because hormones, which are the messengers of the endocrine system, regulate the activity of body cells. The release of hormones into the b ...
Potassium-Sparing Diuretics
Potassium-Sparing Diuretics

... because it can exacerbate acidosis. Potassium-sparing diuretics may cause hyperkalemia. Thiazide diuretics were previously thought to be ineffective when GFR falls below 30 mL/min. More recently, it has been found that thiazide diuretics, which are of little benefit when used alone, can be used to s ...
Chemical Examination of Urine
Chemical Examination of Urine

... • All specimens must be properly labeled – The laboratory request slip must accompany the specimen ...
Job Sharing in the Endomembrane System: Vacuolar
Job Sharing in the Endomembrane System: Vacuolar

... does indeed not contribute to vacuolar acidification under nonstress conditions, in which case we would expect no vacuolar pH difference between vha-a2 vha-a3 and the triple mutant. In contrast, the pH gradient at the tonoplast would be even more reduced in the triple mutant compared with vha-a2 vha- ...
Antibody Binding to Cryptococcus neoformans Impairs Budding by
Antibody Binding to Cryptococcus neoformans Impairs Budding by

... encapsulated microbes, when binding occurs at a certain distance away from the cell. Examples of such direct antimicrobial effects include alterations of microbial metabolic activity, gene expression, quorum sensing, and susceptibility to drugs (2, 3). The physical mechanism(s) of such direct Ab-med ...
LPN-C - Faculty Sites
LPN-C - Faculty Sites

... small spaces and gaps between body structures, cells, and tissues ◦ Transcellular = smallest amount of solution; includes mucus, ocular fluid, sweat, secretions of the genitourinary system, cerebral spinal fluid, pleural solution, pericardial fluid, and peritoneal secretions; separated from other fl ...
Extrapolation of elementary rate constants of P
Extrapolation of elementary rate constants of P

... identified the involvement of a basolateral uptake transporter for both digoxin and loperamide in Caco2 cells, as found previously in MDCKII-hMDR1-NKI cells, due to their low passive permeability. This demonstrates the value of our P-gp structural model as a diagnostic tool in detecting the importan ...
Mapping out the structural changes of natural and pretreated plant
Mapping out the structural changes of natural and pretreated plant

... Fluorescence microscopy [29] and time-of-flight secondary ion mass spectrometry (ToF-SIMS) [30] are also applied in cell wall surface analysis, but the accuracy of component distributions on surface is unsatisfactory due to the limited mapping resolution. AFM recognition imaging by functionalized AF ...
Animal Unit - Misc
Animal Unit - Misc

... clot is slow to form, Small cuts in the skin are usually not a problem, but bleeding in the muscles or joints can be painful and cal lead to serious damage. Today, people with hemophilia are treated as needed with intravenous inject5ions of the missing protein. ...
Population genetics and vaccines - Lectures For UG-5
Population genetics and vaccines - Lectures For UG-5

... Objectives The aim of the course is to •Introduce students to the various disciplines associated with vaccinology including ...
Glycosylation and Sorting of Secretory Proteins in the Endoplasmic
Glycosylation and Sorting of Secretory Proteins in the Endoplasmic

... the secretory glycoprotein Hsp150 (II, III) ....................................... 45 2.1 Hsp150 is secreted in the absence of functional Sec24p (II) .............. 45 2.1.1 Hsp150 is secreted in sec24-1 cells .....................................45 2.1.2 The sorting signal resides in the C-termina ...
Cell Alloimmunization Hemin Controls T Cell Polarization in Sickle
Cell Alloimmunization Hemin Controls T Cell Polarization in Sickle

... Patients with sickle cell disease (SCD) often require transfusions to treat and prevent worsening anemia and other SCD complications. However, transfusions can trigger alloimmunization against transfused RBCs with serious clinical sequelae. Risk factors for alloimmunization in SCD remain poorly unde ...
Integrin cytoplasmic domain-binding proteins
Integrin cytoplasmic domain-binding proteins

... integrins and export from the Golgi, and impairs focal adhesion formation and cell migration (Priddle et al., 1998; Martel et al., 2000), suggest that binding of talin to integrin β tails is important for a variety of integrin functions. However, proof of this hypothesis requires evidence that speci ...
VIRUS-LIKE PARTICLES ELICIT ENHANCED IMMUNITY TO
VIRUS-LIKE PARTICLES ELICIT ENHANCED IMMUNITY TO

University of Groningen Bacillus subtilis at near
University of Groningen Bacillus subtilis at near

... cultures therefore could be of potential use for optimization of these industrial ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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