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Interaction of the endothelial nitric oxide synthase with the CAT
Interaction of the endothelial nitric oxide synthase with the CAT

... intravenous infusion, improves endothelium-dependent vascular relaxation and release of NO [12–15]. This circumstance, in which exogenous arginine drives endothelial NO production even though intracellular levels of arginine are in excess, has been termed the arginine paradox. McDonald et al. [5] ha ...
should we allow organ donation euthanasia?
should we allow organ donation euthanasia?

... for transplantation. Yet there is one currently available, underutilized, potential source of organs. Many patients die in intensive care following withdrawal of life-sustaining treatment whose organs could be used to save the lives of others. At present the majority of these organs go to waste. In ...
Positional Information and the Spatial Pattern of Cellular
Positional Information and the Spatial Pattern of Cellular

... has pointed out, the gradient theory of Child failed to provide a mechanism whereby quantitative differences were translated into pattern. The single attempt to develop a more specific model and one which attempts to get over the limitations just mentioned is that of Rose (1952), but we have found t ...
Cadaverine is Transported into Vibrio vulnificus Through its CadB in
Cadaverine is Transported into Vibrio vulnificus Through its CadB in

... Cadaverine uptake at neutral pH, which requires protonmotive force, is illustrated with Escherichia coli CadB [21], implying its transport by cadaverine/proton symport. The physiological significance for cadaverine uptake at neutral pH, however, remained to be determined. The amino acid residues of ...
Caspases - Essays in Biochemistry
Caspases - Essays in Biochemistry

... the pro-domains in effector caspases (pro-caspases 3, 6 and 7) are short. The long pro-domains contain distinct motifs, including DEDs (death effector domains) present in pro-caspases 8 and 10 and CARDs (caspase-recruitment domains) found in pro-caspases 1, 2, 4, 5 and 9, which are important for the ...
Full Paper - Calcutta Research Group
Full Paper - Calcutta Research Group

... and Kurmis in UP, Gowdas in Karnataka, and Rajputs in Rajasthan, has been largely sustained in the contemporary period, often translating into political power through the medium of party politics (Kothari 1995). In Guntur, the Kammas have found new strategies over the decades post independence, incl ...
PDF
PDF

... 2.4 Determination of Minimum Fungicide Concentration (MFC) The in vitro fungicidal activity (MFC) was determined as described by Díaz-Dellavalle et al. 2011. For MFC determination we used a subsample collected from the microplates previously incubated for 72 hs for fungal development in media broth ...
Intraflagellar transport and the generation of dynamic, structurally
Intraflagellar transport and the generation of dynamic, structurally

... proteins in ciliogenesis – and potential dependence on IFT for regulated entry into cilia – can be dissected. A gated entry into the cilium tended by the IFT machinery? The principle raison d’être for cilia is to concentrate, in an extracytoplasmic space, the machinery responsible for motility and/ ...
Chapter 42B - circulatory
Chapter 42B - circulatory

... NEW AIM: How have different organisms evolved to perform circulation? Circulatory system 3. Two basic true circulatory systems have evolved d. Mammals and birds evolved not from fish, but from reptiles. Hypothesize the number of chambers in a reptilian heart? iii. Amphibians and some reptiles - Have ...
here - The Nebenführ Lab
here - The Nebenführ Lab

... form of hybrid stacks after 20 min of treatment (Figure 4). Interestingly, the average number of cisternae per stack did not change during this period, irrespective of their appearance as Golgi or ER like (Figure 4). The Golgi stacks with at least two cisternae remaining after 10 to 30 min of treatm ...
all-unit-learning-objectives
all-unit-learning-objectives

...  Describe the Jacob-Monod hypothesis of gene action in bacteria using lactose metabolism in E. coli as an example. Use the terms repressor molecule, regulator gene, inducer, operator and structural gene  Describe how genes in control metabolic pathways, using phenylketonuria as an example  Define ...
Tendon development and musculoskeletal assembly: emerging
Tendon development and musculoskeletal assembly: emerging

... establish connectivity and strengthen attachments under tension. Similarly, ECM-dependent interactions between tenocytes and cartilage/bone ensure that tendon-bone attachments form with the appropriate strength for the force required. Recent studies have also established a close lineal relationship ...
COMPLEMENTATION AND PRELIMINARY
COMPLEMENTATION AND PRELIMINARY

... by EMS or UV mutagenesis were examined by phase contrast microscopy. In a standard mating, pair formation occurs within 36 hr after inoculation of nutrient-limited liquid medium (LPN) with vegetative cells. At the end of a 3day incubation, most zygotes are fully rounded and a distinctive “primary zy ...
SEMEN ANALYSIS
SEMEN ANALYSIS

... aqueous solution and the reading of pH is done by comparing the color on the pH strip with the color on the package that the pH strips came in. ...
Proper Folding and Endoplasmic Reticulum to Golgi
Proper Folding and Endoplasmic Reticulum to Golgi

... The abbreviations used are: ER, endoplasmic reticulum; TYR, tyrosinase; WGA, wheat germ agglutinin; TPA, 12-O-tetradecanoylphorbol13-acetate; IBMX, 3-isobutyl-1-methyl xanthine; PBS, phosphatebuffered saline; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1propanesulfonate; Endo H, endoglycosidase H; ...
Modeling the Mechanics of Cell Division: Influence of
Modeling the Mechanics of Cell Division: Influence of

... The cell division cycle is a central process in biology, the essential mechanism whereby cells grow and duplicate (Carlson, 2007). The mechanics of cell division is an essential part of the epigenetic program that supports cellular reproduction in all living organisms (Boal, 2012). The division prog ...
Enhanced intracellular Ca concentrations in and after addition
Enhanced intracellular Ca concentrations in and after addition

... specific proteins, gene expression and stress signals (Norris et al. 1996; Jones et al. 2002; Dominguez, 2004). Cytosolic Ca2+ concentration in E. coli is similar to that in eukaryotic systems suggesting that bacteria regulate Ca2+ homeostasis and that the Ca2+ gradient could be used for transmittin ...
as a PDF
as a PDF

... polarize in the mediolateral axis, orient and stabilize lamellipodia, and exert traction on neighboring cells [10-12]. These behaviors propagate through the tissue from anterior to posterior as gastrulation proceeds [8, 11]. The need for reliable coordination of these cell behaviors suggests that se ...
Article - An Ocean of Viruses
Article - An Ocean of Viruses

... so-called “viral shunt,” by which viral killing of microbial hosts redirects carbon and nutrients away from larger organisms and back toward other microorganisms.4,5 Furthermore, researchers analyzing oceanic life have discovered many novel viruses that defy much of the conventional wisdom about wha ...
Rheb and mammalian target of rapamycin in mitochondrial
Rheb and mammalian target of rapamycin in mitochondrial

... from the generation of ATP, the electrochemical gradient is also used for import of proteins and metabolites that are used to sustain the function of the mitochondria themselves. The function of mitochondria also includes the formation of building blocks for amino acid and fatty acid synthesis, stor ...
Association between border cell responses and localized root
Association between border cell responses and localized root

... sativum). Aphanomyces euteiches invades the root system leading to a complete arrest of root growth and ultimately to plant death. To date, disease control measures are limited to crop rotation and no resistant pea lines are available. The present study aims to get a deeper understanding of the earl ...
The maize mutant narrow sheath fails to establish
The maize mutant narrow sheath fails to establish

... development is the subdivision of organ primordia into developmental compartments (Garcia-Bellido et al., 1973). First identified by their boundaries of cell-lineage restriction, compartments were later shown to arise via the expression of transcriptional activators in particular regions (e.g. Kornb ...
Fcg Receptor Deficiency Attenuates Diabetic Nephropathy
Fcg Receptor Deficiency Attenuates Diabetic Nephropathy

... susceptibility to oxidation, and lipid fatty acid composition are critical in the formation of immune complexes in type 2 diabetic patients.8 Indeed, oxidized LDL–containing immune complexes represent a good marker to evaluate the effect of humoral immunity in diabetes complications.8,11,12 At sites ...
PDF
PDF

... projected medially and connected with the central aspect of the ganglion by 60 hour clonal age. The AA axon fiber(s) originated near the anterior, lateral aspect of the ganglion and appeared to project processes both medially and laterally to form the AA segmental nerve. At ~60 hours clonal age, axo ...
Positive and Negative Regulation of Muscle Cell
Positive and Negative Regulation of Muscle Cell

... (Promega Corp., Madison, WI). The resulting plasmid, pGEM-7Ztwhh5.2, containing the 5.2-kb twhh promoter, was partially digested with BstXI and EcoRI to release the DNA insert. The EcoRI site at the 59 end of the insert was then blunted by Klenow DNA polymerase and subcloned into the pBluescript-SK ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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