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Role of Sulfhydryl Sites on Bacterial Cell Walls in the
Role of Sulfhydryl Sites on Bacterial Cell Walls in the

... functional groups to be the important metal binding groups on bacterial cell walls. However, our preliminary X-ray absorption spectroscopy studies indicated the presence of sulfhydryl groups and identified their chemical state in bacteria and cell surface isolates. Our studies also indicate that cha ...
The Australian Stem Cell Handbook
The Australian Stem Cell Handbook

... tissue, replace missing tissue and repair the effects of disease or injury. Experimental stem cell therapy is being developed for many common diseases, including blindness, stroke, arthritis, multiple sclerosis and heart attacks. Important research is being undertaken by reputable hospital, biotechn ...
The tRNA methyltransferase Dnmt2 is required foraccurate
The tRNA methyltransferase Dnmt2 is required foraccurate

... cells within the bone marrow of 8-day-old Dnmt2/ mice, we could not observe any changes in frequencies or numbers of differentiated peripheral blood cell populations, neither in young (8 days) nor in adult (8 months) mice (Fig EV1A and B). We therefore asked whether the ratio of bone marrow LSK ce ...
a Gene Encoding a Putative Signal Transduction Receptor in
a Gene Encoding a Putative Signal Transduction Receptor in

... The gene encoding this RNA was designated ASI2, for anlagen stage induced 2. Because the cloned DNA contained only about 350 bp of the ASI2 open reading frame, two rounds of inverse PCR were done to obtain the full ASI2 gene (Materials and Methods). Inverse PCR products were cloned and sequenced (G ...
Honors Biology Differentiation
Honors Biology Differentiation

... Honors Biologyvs. deuterostome protostome ...
CHAPTER 35: PROTISTS
CHAPTER 35: PROTISTS

... a. Fuse with lysosomes containing digestive enzymes b. Digested molecules absorbed across vacuole membrane c. Food vacuole decreases in size F. Reproduction 1. Generally reproduce asexually 2. Sexual reproduction occurs only in time of stress 3. Asexual reproduction involved mitosis, but may be quit ...
Polo kinase and progression through M phase in Drosophila
Polo kinase and progression through M phase in Drosophila

... The polo-like kinases have been shown to follow similar dynamic patterns of localization during progression through mitosis essentially in all metazoans examined. Perhaps the most dramatic illustration of the dynamics of the protein kinase has come from studies by Claudio Sunkel’s laboratory (Moutin ...
The Identity of Proteins Associated with a Small Heat Shock Protein
The Identity of Proteins Associated with a Small Heat Shock Protein

... component of the cellular chaperone network that becomes particularly important under conditions of severe stress involving protein aggregation. Although the ability of sHSPs to interact with denatured model substrates in vitro has been studied extensively, the characteristics of cellular sHSP subst ...
Evidence for Functional Platelet-derived Growth Factor Receptors
Evidence for Functional Platelet-derived Growth Factor Receptors

... extra glucose (0.09 g/liter) and pyruvate (0.616 g/liter). BALB/C-3T3 cells were grown in Dulbecco's MEM (Biofluids, Inc., Rockville, MD). FBS was purchased from Biofluids. [3H]Thymidine (16 Ci/mmol), carrier-free Na125l, [3H]aminoisobutyric acid, and [-y-^PJATP were obtained from New Eng land Nucle ...
Evidence for Functional Platelet-derived Growth Factor Receptors
Evidence for Functional Platelet-derived Growth Factor Receptors

... extra glucose (0.09 g/liter) and pyruvate (0.616 g/liter). BALB/C-3T3 cells were grown in Dulbecco's MEM (Biofluids, Inc., Rockville, MD). FBS was purchased from Biofluids. [3H]Thymidine (16 Ci/mmol), carrier-free Na125l, [3H]aminoisobutyric acid, and [-y-^PJATP were obtained from New Eng land Nucle ...
the morphogenetic function of a vestigial organ in the chick
the morphogenetic function of a vestigial organ in the chick

... plate; again pronephric tubules may be developed (PL I, fig. 2). In these embryos there is, however, always a little neural tissue included with the lateral plate and intermediate cell mass, since the neural plate extends, when it is flat, slightly further laterally than the somitic mesoderm. The po ...
RNA-binding Proteins TIA-1 and TIAR Link the
RNA-binding Proteins TIA-1 and TIAR Link the

... HE general translational arrest that accompanies environmental stress is initiated by the phosphorylation of eIF-2a, an essential component of the heterotrimeric eIF-2 complex that loads the initiator tRNA (Met-tRNAMet) onto the 40S ribosomal subunit (Berlanga et al., 1998; Gray and Wickens, 1998; S ...
Autonomic Nervous System
Autonomic Nervous System

... b. Adrenergic receptors -- bind NE. - two major classes are alpha (usually stimulatory), and beta (usually inhibitory); found in effector cells of postganglionic sympathetic fibers. C. Effects of parasympathetic and sympathetic divisions on various organs -- see Table ...
Severe osmotic compression triggers a slowdown of
Severe osmotic compression triggers a slowdown of

... association rates of several proteins inside the cell. Molecular crowding has received little attention in living cells, although it is usually invoked to explain why biochemical reactions rates may vary in vivo and in vitro (26–29). For instance, the diffusion coefficient of the green fluorescent pro ...
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to
A Series of Ubiquitin Binding Factors Connects CDC48/p97 to

... (ubiquitin ligase) finally binds both the E2 and a substrate and catalyzes the transfer of ubiquitin to the substrate. Ubiquitin itself is often a substrate for further ubiquitylation, and proteins modified by such multiubiquitin chains are preferentially targeted for degradation by the proteasome. ...
Differences in Whole-Cell and Single
Differences in Whole-Cell and Single

... Because single-channel recordings are likely to reflect the differences in the kinetics observed in whole-cell measurements, we recorded singlechannel activity in excised outside-out patches isolated from both Thlaspi spp. (Fig. 5). However, there were no significant differences in the steady-state ...
Virtual Frog Dissection Lab
Virtual Frog Dissection Lab

... Click on Menu on the bottom at the bottom of the page when you are ready to move on to the next section External Anatomy 5. Orientation. Is it possible to tell if a frog is male or female by external appearance? 6. Skin. What does the mucus do for the skin? 7. Head. Where are the tympanic membranes ...
document
document

... Sugar gone awry cont’d In some people, this cycle doesn't work properly. People with type 1 diabetes (once called insulin-dependent or juvenile diabetes) don't make enough insulin, so their cells can't absorb sugar. People with type 2 diabetes (once called non-insulin dependent or adult onset diabe ...
New Trends in Evolutionary Computation
New Trends in Evolutionary Computation

... analogue behaviour abstracted away by digital designers, we can enable the generation of more efficient circuits, and perhaps even aid the evolutionary process through improved evolvability. Thompson’ s (1996) tone discriminator is an example of such work. Parts of this circuit behave digitally, as ...
Chapter 42 – Circulation and Gas Exchange
Chapter 42 – Circulation and Gas Exchange

... Cardiac output is the volume of blood pumped per minute, and it depends on two factors: the rate of contraction or heart rate (number of beats per second) and stroke volume, the amount of blood pumped by the left ventricle in each contraction. ...
The Induction of 72-kD Gelatinase in T Cells upon Adhesion to
The Induction of 72-kD Gelatinase in T Cells upon Adhesion to

... endothelial cells and cocultured for 5 h at 37°C, 8% CO2, during which time T cell adhesion to the endothelial cell monolayer peaked. As a control, T cells (2 x 106/ml) were incubated in serum-free DME for 5 h at 37°C in suspension either in 50-ml polypropylene tubes or in T-75 culture flasks that w ...
The metabolism and functions of inositol pentakisphosphate and
The metabolism and functions of inositol pentakisphosphate and

... InsP, and InsP,, but not Ins/-’,, into the nucleus tractus solitarius (a discrete brain stem nucleus implicated in cardiovascular regulation) results in dose-dependent, reversible changes in heart rate and blood pressure. Furthermore, they showed that [3H]lnsl’, and [‘HIInsP, are synthesized in inta ...
Exam 2
Exam 2

... A.residual volume B.tidal volume C.vital capacity 92. The type of respiration in which ATP is produced within a living cell is called: A.breathing B.external respiration C.internal respiration D.cellular respiration 93. The highest carbon dioxide concentration (partial pressure CO2) is found in the: ...
Regulation of multiple target genes by miR-1 and miR
Regulation of multiple target genes by miR-1 and miR

... Myogenic Regulatory Factors (MRFs) in vivo (Hinits et al., 2011; Sweetman et al., 2008; Sweetman et al., 2006) consistent with the finding that MyoD and myogenin bind to regions upstream of miR-1 and induce its expression in cell culture (Rao et al., 2006; van Rooij et al., 2008). Recently we demons ...
Chapter 1 - Utrecht University Repository
Chapter 1 - Utrecht University Repository

... Although the name early endosome implies it to be a single compartment, this clearly is an oversimplification. This was initially demonstrated by Maxfield and coworkers in Chinese hamster overy (CHO) cells using FITC-transferrin as endocytic tracer (Yamashiro et al., 1984). They observed that transf ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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