Functions of the Arabidopsis kinesin superfamily of microtubule
... potentially could contribute to bundling of CMTs. However, the atk5 mutant has normal CMT organization (Ambrose et al. 2005), indicating that it does not play an essential role in CMT organization. CMTs are dynamic at both ends and their dynamic properties are important for array organization (Shaw ...
... potentially could contribute to bundling of CMTs. However, the atk5 mutant has normal CMT organization (Ambrose et al. 2005), indicating that it does not play an essential role in CMT organization. CMTs are dynamic at both ends and their dynamic properties are important for array organization (Shaw ...
Identification of genes controlling germ cell migration and embryonic
... Sb genotype. However, these ‘lines’ usually contained only 1-2 ‘escaper’ progeny and did not pose a serious problem to the screening procedure. Lines that were kept for further analysis (see below) were inspected for ‘escaper’ flies and, if necessary, virgin females and males of the desired genotype ...
... Sb genotype. However, these ‘lines’ usually contained only 1-2 ‘escaper’ progeny and did not pose a serious problem to the screening procedure. Lines that were kept for further analysis (see below) were inspected for ‘escaper’ flies and, if necessary, virgin females and males of the desired genotype ...
Relative Potencies of Individual Polycyclic Aromatic Hydrocarbons to Induce Dioxinlike
... capable of inducing dioxinlike responses in vitro in both fish (Villeneuve et al., 1998; Bols et al., 1999, Fent and Bätscher, 2000; Behrens et al., 2001; Jung et al., 2001) and mammalian cell lines (Willett et al., 1997; Clemons et al., 1998; Jones and Anderson, 1999). PAHs have also been shown to ...
... capable of inducing dioxinlike responses in vitro in both fish (Villeneuve et al., 1998; Bols et al., 1999, Fent and Bätscher, 2000; Behrens et al., 2001; Jung et al., 2001) and mammalian cell lines (Willett et al., 1997; Clemons et al., 1998; Jones and Anderson, 1999). PAHs have also been shown to ...
Ion homeostasis, channels, and transporters: an update on cellular
... Because the actual transport of ions through channels does not require a defined sequence of energetic interactions between the transport protein (the channel) and the transported ions, the rate of ion transport through channel proteins is usually many times faster than the rate of transport through ...
... Because the actual transport of ions through channels does not require a defined sequence of energetic interactions between the transport protein (the channel) and the transported ions, the rate of ion transport through channel proteins is usually many times faster than the rate of transport through ...
The relative roles of specific N- and C
... [32P]phosphoric acid (ICN, Costa Mesa, CA) for 4 hours. Collection of mitotic cells, immunoprecipitation of vimentin from cell lysates, and two-dimensional phosphopeptide mapping were carried out as detailed previously (Chou et al., 1990). Purification of p37 vimentin kinase The two protein kinase a ...
... [32P]phosphoric acid (ICN, Costa Mesa, CA) for 4 hours. Collection of mitotic cells, immunoprecipitation of vimentin from cell lysates, and two-dimensional phosphopeptide mapping were carried out as detailed previously (Chou et al., 1990). Purification of p37 vimentin kinase The two protein kinase a ...
PDF - Bezanilla Lab
... of actin-based structures responsible for diverse cellular processes. Because multicellular organisms contain large formin gene families, establishing the physiological functions of formin isoforms has been difficult. Using RNAi, we analyzed the function of all 9 formin genes within the moss Physcom ...
... of actin-based structures responsible for diverse cellular processes. Because multicellular organisms contain large formin gene families, establishing the physiological functions of formin isoforms has been difficult. Using RNAi, we analyzed the function of all 9 formin genes within the moss Physcom ...
PDF
... and commitment of the macrophage to phagocytosis. This study utilized 5.6 mm polystyrene particles with two different Fc densities and found that a high Fc density led to complete closure of the phagosome around the microparticle while a low Fc density resulted in binding of the particle to the macr ...
... and commitment of the macrophage to phagocytosis. This study utilized 5.6 mm polystyrene particles with two different Fc densities and found that a high Fc density led to complete closure of the phagosome around the microparticle while a low Fc density resulted in binding of the particle to the macr ...
Designing PPT Presentation
... osmolality of the extracellular fluid (ECF) is approximately equal to that of the intracellular fluid (ICF). • it shows that changes in ECF osmolality have a great effect on ICF osmolality changes that can cause problems with normal cell functioning and volume. • If the ECF were to become too hypoto ...
... osmolality of the extracellular fluid (ECF) is approximately equal to that of the intracellular fluid (ICF). • it shows that changes in ECF osmolality have a great effect on ICF osmolality changes that can cause problems with normal cell functioning and volume. • If the ECF were to become too hypoto ...
trisphosphate specifically interacts with the phox homology domain
... (PtdIns) were incubated with 10 ng of the purified GST-PLD PX domains in 150 l buffer A (50 mM Hepes-NaOH pH 7.5, 3 mM MgCl2, 2 mM CaCl2, 3 mM EGTA, and 80 mM KCl) as previously described (Kim et al., 1998). After incubation at 37°C for 15 minutes, the reaction mixtures were centrifuged at 300,000 ...
... (PtdIns) were incubated with 10 ng of the purified GST-PLD PX domains in 150 l buffer A (50 mM Hepes-NaOH pH 7.5, 3 mM MgCl2, 2 mM CaCl2, 3 mM EGTA, and 80 mM KCl) as previously described (Kim et al., 1998). After incubation at 37°C for 15 minutes, the reaction mixtures were centrifuged at 300,000 ...
The Human Body
... right ventricle contracts, blood is pumped through a valve and into the pulmonary artery (3). From there, blood flows into the lungs where it picks up oxygen (4). The now oxygen-rich blood is carried back to the left atrium through the pulmonary veins (5). When the left atrium contracts, blood goes ...
... right ventricle contracts, blood is pumped through a valve and into the pulmonary artery (3). From there, blood flows into the lungs where it picks up oxygen (4). The now oxygen-rich blood is carried back to the left atrium through the pulmonary veins (5). When the left atrium contracts, blood goes ...
Japanese encephalitis virus replication is negatively regulated by
... to JEV infection we monitored the processing of LC3-I to its lipidated membrane-bound form LC3-II. The mouse neuroblastoma cell line Neuro2a was transfected with GFP-LC3 and set up for serum-starvation or JEV-infection (Fig. 1). In mockinfected Neuro2a cells, GFP-LC3 shows a diffuse distribution (Fi ...
... to JEV infection we monitored the processing of LC3-I to its lipidated membrane-bound form LC3-II. The mouse neuroblastoma cell line Neuro2a was transfected with GFP-LC3 and set up for serum-starvation or JEV-infection (Fig. 1). In mockinfected Neuro2a cells, GFP-LC3 shows a diffuse distribution (Fi ...
Positive Regulation of IκB Kinase Signaling by Protein
... These functional results, together with data shown, further reinforce the model that PP2A binds to IKK, facilitating the induction of IκB kinase activity, targeted degradation of IκB, and release of NF-κB to its nuclear site of action. Instead, our studies support the idea that this region of IKKγ i ...
... These functional results, together with data shown, further reinforce the model that PP2A binds to IKK, facilitating the induction of IκB kinase activity, targeted degradation of IκB, and release of NF-κB to its nuclear site of action. Instead, our studies support the idea that this region of IKKγ i ...
TNF-induced endothelial barrier disruption: beyond actin and Rho
... in pathogen clearance and the repair of damaged tissue. It constitutes an alteration of tissue homeostasis in reaction to stress, which is also detrimental to tissue function. An accurate spatiotemporal restriction of the inflammatory response is therefore crucial for preventing uncontrolled, system ...
... in pathogen clearance and the repair of damaged tissue. It constitutes an alteration of tissue homeostasis in reaction to stress, which is also detrimental to tissue function. An accurate spatiotemporal restriction of the inflammatory response is therefore crucial for preventing uncontrolled, system ...
Why Belzer UW® Cold Storage Solution?
... compared to…UW hydroxyethyl starch [HES].” 2 “Various impermeants have been used to sustain the oncotic gradient across the cell membrane and prevent oedema. … UW pioneered the use of lactobionate and raffinose…Lactobionate remains the impermeant choice of having been successfully used in preservati ...
... compared to…UW hydroxyethyl starch [HES].” 2 “Various impermeants have been used to sustain the oncotic gradient across the cell membrane and prevent oedema. … UW pioneered the use of lactobionate and raffinose…Lactobionate remains the impermeant choice of having been successfully used in preservati ...
PLANT IN VITRO TRANSCRIPTION SYSTEMS
... initiation was most active in the presence of 0.5-1 mM of Mn2+; far less activity was obtained using Mg2+ (44). The chromatin extract also supported accurate transcription initiation of a circular DNA containing the CaMV 35S promoter (44). Using this in vitro system with the 35S promoter containing ...
... initiation was most active in the presence of 0.5-1 mM of Mn2+; far less activity was obtained using Mg2+ (44). The chromatin extract also supported accurate transcription initiation of a circular DNA containing the CaMV 35S promoter (44). Using this in vitro system with the 35S promoter containing ...
Cytoskeletal Drugs and Gravity-Induced Lateral Auxin Transport in
... coleoptiles of about 15 mm in length were placed, with the gravity vector pointing from tip towards base, in Eppendorf tubes containing water (control) or aqueous solutions of various cytoskeletal drugs, and kept for 30 min in the same orientation. They were then inverted for various time intervals, ...
... coleoptiles of about 15 mm in length were placed, with the gravity vector pointing from tip towards base, in Eppendorf tubes containing water (control) or aqueous solutions of various cytoskeletal drugs, and kept for 30 min in the same orientation. They were then inverted for various time intervals, ...
Chapter 26 – The Biomanufacturing of Biotechnology Products
... synthesis the biologic product resulting from a living system is not as an exact science as chemistry. The long-existing paradigm for biologics was “the process is the product” and any variation in the process could impart a change in the product’s safety and efficacy. Although today’s raw materials ...
... synthesis the biologic product resulting from a living system is not as an exact science as chemistry. The long-existing paradigm for biologics was “the process is the product” and any variation in the process could impart a change in the product’s safety and efficacy. Although today’s raw materials ...
eXtra Botany - Oxford Academic
... many mosses resembles that of vascular plant sporophytes, though with very different anatomy (e.g. no intercellular gas spaces or stomata, hydrome rather than xylem, and leptome rather than phloem). Long-distance nutrient transport in the Charales, apparently involving cytoplasmic streaming (Raven, ...
... many mosses resembles that of vascular plant sporophytes, though with very different anatomy (e.g. no intercellular gas spaces or stomata, hydrome rather than xylem, and leptome rather than phloem). Long-distance nutrient transport in the Charales, apparently involving cytoplasmic streaming (Raven, ...
Chapter 3.05
... 6-O-sulfation of the second Gal residue, inhibit the transfer of a GlcA residue by GlcAT-I.24 Thus, the sulfation of specific positions of the two Gal residues may regulate the expression level of GAGs by affecting the activity of GlcAT-I, although further experiments including the cloning of sulfot ...
... 6-O-sulfation of the second Gal residue, inhibit the transfer of a GlcA residue by GlcAT-I.24 Thus, the sulfation of specific positions of the two Gal residues may regulate the expression level of GAGs by affecting the activity of GlcAT-I, although further experiments including the cloning of sulfot ...
Health Promotion Resources Models and displays catalogue Reay
... Pair of spectacles with lenses designed to simulate the effect created by alcohol and other drugs. The result is a realistic sensation of dizziness, reduced and eye coordination and a diminished ability to perform motor tasks. Suggested use with condom demonstrator models. DW Eyes Game Kit (with gog ...
... Pair of spectacles with lenses designed to simulate the effect created by alcohol and other drugs. The result is a realistic sensation of dizziness, reduced and eye coordination and a diminished ability to perform motor tasks. Suggested use with condom demonstrator models. DW Eyes Game Kit (with gog ...
1 Electrophysiology and pacemaker function of the - AJP
... interaction between nodal cells and nodal-atrial cells. A detailed analysis of the ionic mechanisms generating automaticity requires studies at the single cell level, but to fully understand the complexity of the SAN physiology it is important to consider that each cell exists as an element of a com ...
... interaction between nodal cells and nodal-atrial cells. A detailed analysis of the ionic mechanisms generating automaticity requires studies at the single cell level, but to fully understand the complexity of the SAN physiology it is important to consider that each cell exists as an element of a com ...
Perturbation of - Circulation Research
... tant role in the genesis of congestive heart failure (CHF).11–14 Therefore, a better understanding of the molecular mechanisms by which the UPS function is regulated in the heart may provide critical information for developing novel therapeutic strategies to treat the related cardiac disorders or to ...
... tant role in the genesis of congestive heart failure (CHF).11–14 Therefore, a better understanding of the molecular mechanisms by which the UPS function is regulated in the heart may provide critical information for developing novel therapeutic strategies to treat the related cardiac disorders or to ...
Phosphorylation of eIF2α in response to 26S proteasome inhibition
... reported to induce apoptosis in several different experimental systems [14–17], our results indicate that loss of protein synthesis activity after short periods of inhibition of the 26S proteasome is not accompanied by any signs of induction of apoptosis. Instead, we show that inhibition of the 26S ...
... reported to induce apoptosis in several different experimental systems [14–17], our results indicate that loss of protein synthesis activity after short periods of inhibition of the 26S proteasome is not accompanied by any signs of induction of apoptosis. Instead, we show that inhibition of the 26S ...
Acute Renal Failure - The Podiatry Institute
... prolong renal hypoperfusion can lead to ischemic acute tubular necrosis with significant increase in morbidity ...
... prolong renal hypoperfusion can lead to ischemic acute tubular necrosis with significant increase in morbidity ...
The Regulation of S Phase Initiation by p27Kip1 in NIH3T3 Cells
... and finally stained with DAPI (10 µg/ml). Images of each fluorochrome were taken with a cooled CCD camera (Roper Scientific) with the appropriate filters (Chroma) of the same area of cells. The shading of each image was corrected with a uniformly stained specimen; and the DAPI image was utilized for ...
... and finally stained with DAPI (10 µg/ml). Images of each fluorochrome were taken with a cooled CCD camera (Roper Scientific) with the appropriate filters (Chroma) of the same area of cells. The shading of each image was corrected with a uniformly stained specimen; and the DAPI image was utilized for ...