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Phage adsorption and lytic propagation in
Phage adsorption and lytic propagation in

... phage propagation in lactic acid bacteria. However, this thematic was studied in E. coli phages. In this sense and in concordance with our results, it was demonstrated E. coli phages were not able to propagate in starved cells in a mineral salt medium [13]. In particular, some authors reported phage ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine

... SC1siRNA-2) into cultured rat NSCs markedly decreased the expression of endogenous SC1 protein, as detected on Western blots (Fig. 2A). Moreover, SC1 immunoreactivity was low or undetectable in siRNA-transfected NSCs, compared to control siRNA-transfected NSCs (Fig. 2B). Successfully transfected cel ...
Signaling tip growth in plants Zhenbiao Yang
Signaling tip growth in plants Zhenbiao Yang

... cell polarity development in yeast and mammals [1,2••,3•], a general model for tip growth pathways is proposed (Figures 1 and 2). Tip growth begins with the selection and establishment of a cortical site of the cell at which vesicles will dock and fuse [2••]. These processes require a signaling netw ...
Induction of type-IIA secretory phospholipase A2 in animal models of
Induction of type-IIA secretory phospholipase A2 in animal models of

... factor, leukotrienes and prostaglandins [5, 6]. A number of studies suggest that sPLA2-IIA is involved in the pathogenesis of inflammatory diseases including ARDS. Indeed, a clinical trial demonstrated an increased sPLA2-IIA activity in bronchoalveolar lavage (BAL) fluid of patients with ARDS whose ...
Identification of plant cytoskeleton-interacting proteins
Identification of plant cytoskeleton-interacting proteins

... XL1 Blue Escherichia coli competent cells was highly efficient resulting in more than 100 000 colonies, 60 000 of which were collected. In a control experiment, DNA made from a pool of 2500 clones and a mixture of the empty vectors was transfected into 293T cells. It was found that although the tra ...
Influence of Notch on dorsoventral
Influence of Notch on dorsoventral

... Cohen, 2003; O’Keefe and Thomas, 2001; Rauskolb et al., 1999). Two models are based on the conventional view that compartmentalization requires the establishment of distinct, compartment-specific cell affinities. In one version (Micchelli and Blair, 1999), Notch activation confers a distinct boundar ...
UvA-DARE (Digital Academic Repository) Mitochondria in neutrophil
UvA-DARE (Digital Academic Repository) Mitochondria in neutrophil

... Mcl-1 in neutrophils seems to be inhibition of Bax translocation 5, but this thesis has demonstrated that the role of Bfl-1 in the apoptotic process is far from obvious. The function of Bax itself is also not directly clear. This protein is a main contributor to the formation of the PTP. However, it ...
The role of active oxygen species in plant signal transduction
The role of active oxygen species in plant signal transduction

... hand, in leaf tissue exposed to HL intensities, PR protein accumulation was observed in the absence of any pathogenic stimulus. Induction of PR proteins was most prominent in necrotic leaves, but could be observed after 2 weeks in the upper leaves without any macroscopic damage [10]. This observatio ...
www .bio x.com Anti-VIRAL HAEMORRHAGIC SEPTICAEMIA
www .bio x.com Anti-VIRAL HAEMORRHAGIC SEPTICAEMIA

... thousand tons of trout are lost to this disease each year. Three distinct serotypes of this virus have been identified. The rainbow trout (Oncorhynchus mykiss) appears to be very sensitive to this virus. However, other Salmonidae, such as the brown trout (Salmo trutta fario L.), grayling (Thymallus ...
RCAI Annual Report 2006 (PDF 5.6M)
RCAI Annual Report 2006 (PDF 5.6M)

... rather than competing with the universities, can complement the university research system. What is most significant with RCAI is the focus on young investigators at the beginning of their career. Well over two-thirds of the research laboratories at the Center are lead by young investigators, many o ...
bZIP transcription factor interactions regulate DIF
bZIP transcription factor interactions regulate DIF

... this study, we describe the identification of a DimA-related bZIP transcription factor, DimB. Rapid changes in the subcellular localisation of both DimA and DimB in response to DIF-1 suggest that they are directly downstream of the DIF-1 signal. Genetic and biochemical interactions between DimA and D ...
Synthesis of a Viral Protein with Molecular
Synthesis of a Viral Protein with Molecular

... healthy individuals and patients with neoplastic diseases (23). However, it was convincingly shown that these anti-gp70 anti bodies are formed in response to cellular modification of glycoproteins rather than as a consequence of exposure to virus (2, 22). Human sera in those studies appeared not to ...
S1P promotes murine progenitor cell egress and
S1P promotes murine progenitor cell egress and

Human Dcp2: a catalytically active mRNA decapping enzyme
Human Dcp2: a catalytically active mRNA decapping enzyme

... the product generated by hDcp2 (Figure 3A) but migrate at the expected positions compared to unlabelled standards (m7GMP and inorganic phosphate, Pi). To prove de®nitively that the product of the reaction catalysed by hDcp2 contains two phosphates, we incubated the reaction products with nucleotide ...
Heart Chambers - Cloudfront.net
Heart Chambers - Cloudfront.net

... increases Observe this trend in the figure at right The typical amphibian lung is little more than a sac with ridges Reptilian lungs are often divided into a series of large and small chambers that increase the surface area available for gas exchange In mammals, the lungs branch extensively, and the ...
What do colony patterns mean? - James A. Shapiro
What do colony patterns mean? - James A. Shapiro

... medium formed by wild-type Bacillus subtilis strain 3610: detection of different cellular morphologies and constellations of cells as the complex architecture develops. Microbiology 150:1839-49. ...
459 Resources
459 Resources

... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
p53 Pathway in Renal Cell Carcinoma Is Repressed by a Dominant
p53 Pathway in Renal Cell Carcinoma Is Repressed by a Dominant

... HeLa (cervical carcinoma), A293 (embryonic kidney epithelial cells transRenal cell carcinoma (RCC) accounts for approximately 85% of all formed with DNA of adenovirus type 5), and LNCaP (prostate adenocarcikidney cancers. Approximately 30,000 patients in the United States noma)] were purchased from ...
Anatomy & Physiology
Anatomy & Physiology

... 1. Drained by two sets of lymphatic vessels a. Lymphatics that drain the skin over the breast with the exception of the areola & nipple b. Lymphatics that drain the substance of the breast as well as the skin of the areola & nipple 2. Superficial vessels coverage to form a diffuse, cutaneous lymphat ...
Imaging Cell Wall Architecture in Single Zinnia
Imaging Cell Wall Architecture in Single Zinnia

... secondary walls and undergo programmed cell death, which results in the removal of all cell contents, leaving behind a “functional corpse” (Roberts and McCann, 2000; Fukuda, 2004). In broad terms, the primary cell wall of higher plants is mainly composed of three types of polysaccharides: cellulose, ...
Nucleation and Dynamics of Golgi-derived Microtubules
Nucleation and Dynamics of Golgi-derived Microtubules

... originates at the Golgi itself, which in this case functions as a MT-organizing center (MTOC). Golgi-derived MTs serve important roles in post-Golgi trafficking, maintenance of Golgi integrity, cell polarity and motility, as well as cell type-specific functions, including neurite outgrowth/branching ...
Keratin Alterations during Embryonic Epidermal Differentiation: A
Keratin Alterations during Embryonic Epidermal Differentiation: A

... which were allowed to attach to the surface of the tissue culture dish . In the case of 29-d embryos, keratinocyte colonies developed from trypsinized cell suspensions of embryonic skin . Cultures of fibroblasts were obtained by plating trypsin-disaggregated cell suspensions of embryonic skin from e ...
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and

... (unmodified) OPN to identify MAbs that specifically recognize either the native or the recombinant form. Antibodies specific only for the native form would be candidates for MAbs recognizing an epitope that is post-translationally modified; antibodies specific only for the recombinant form would pre ...
Computational optogenetics: A novel continuum framework for the
Computational optogenetics: A novel continuum framework for the

... The human heart propels over 7000 l of blood through the body daily, beating more than 40 million times a year. It is a remarkably efficient, durable, and reliable mechanical pump, precisely regulated by spatially and temporally varying electrical and chemical fields. Disturbed conduction and uncoordi ...
Cell-cell communication mediated by the CAR subgroup of
Cell-cell communication mediated by the CAR subgroup of

... In crystals the full extracellular region of CAR forms U-shaped homodimers through the binding of their N-terminally located Ig domains which is reminiscent to JAM-A homodimers (Kostrewa et al., 2001; Patzke et al., 2010; Prota et al., 2003; Verdino et al., 2010). D1 and D2 associate in a head-to-ta ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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