Chemical Microscopy Applied to Biological Systems
... on the membrane surfaces of epithelial cells were probed by the Pfister research group (37). Numerous applications of confocal microscopy are directed toward counting or size measurements. Kukavica-Ibrulj et al. described an immunofluorescence system for detection and quantitation of hepatitis A vir ...
... on the membrane surfaces of epithelial cells were probed by the Pfister research group (37). Numerous applications of confocal microscopy are directed toward counting or size measurements. Kukavica-Ibrulj et al. described an immunofluorescence system for detection and quantitation of hepatitis A vir ...
The essential role of anionic transport in plant cells
... nutrition. Notwithstanding, a lot is still unknown about the specific function of these transporters, their specific localization, or molecular nature. Here the fundamental roles of anionic transport in plant cells are reviewed. Special attention will be paid to them in the control of pollen tube gr ...
... nutrition. Notwithstanding, a lot is still unknown about the specific function of these transporters, their specific localization, or molecular nature. Here the fundamental roles of anionic transport in plant cells are reviewed. Special attention will be paid to them in the control of pollen tube gr ...
Animal Biology 56(4)
... Abstract—The vertebrate cranium consists of three parts: neuro-, viscero- and dermatocranium, which differ in both developmental and phylogenetic origin. Traditionally, developmental origin has been used as a criterion for homology, but this becomes problematic when skull elements such as the pariet ...
... Abstract—The vertebrate cranium consists of three parts: neuro-, viscero- and dermatocranium, which differ in both developmental and phylogenetic origin. Traditionally, developmental origin has been used as a criterion for homology, but this becomes problematic when skull elements such as the pariet ...
A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with
... methods were approved by the Weill Medical College of Cornell University Institutional C are and Use Committee. Six Sprague Dawley rats (Taconic, Germantown, N Y) were used in the light microscopy immunocytochemical experiments. All rats were anesthetized with Nembutal (150 mg / kg, i.p.) and sequen ...
... methods were approved by the Weill Medical College of Cornell University Institutional C are and Use Committee. Six Sprague Dawley rats (Taconic, Germantown, N Y) were used in the light microscopy immunocytochemical experiments. All rats were anesthetized with Nembutal (150 mg / kg, i.p.) and sequen ...
Meiotic sex chromosome inactivation
... spermatogenesis, spermatogonial stem cells divide by mitosis to generate progressively more-differentiated progeny; here, genes on the X and Y chromosomes are transcriptionally active. One study (Wang et al., 2001) has revealed that a disproportionately large number of spermatogonial-specific transc ...
... spermatogenesis, spermatogonial stem cells divide by mitosis to generate progressively more-differentiated progeny; here, genes on the X and Y chromosomes are transcriptionally active. One study (Wang et al., 2001) has revealed that a disproportionately large number of spermatogonial-specific transc ...
Document
... secretions [4]. Th1 cells produce IL-2 and IFN- γ that is associated with CM1 and protection against to intracellular pathogens such as leishmania [5], whereas activation of a Th2 cells produce IL-4, IL-5, IL-6, IL-10, and IL-13 and IgG2a that is accompanied with humoral immunity [6,7,8]. There are ...
... secretions [4]. Th1 cells produce IL-2 and IFN- γ that is associated with CM1 and protection against to intracellular pathogens such as leishmania [5], whereas activation of a Th2 cells produce IL-4, IL-5, IL-6, IL-10, and IL-13 and IgG2a that is accompanied with humoral immunity [6,7,8]. There are ...
BOTANICAL BRIEFING. Signalling between Pathogenic Rust Fungi
... because of the inter-organismal co-operation that must be required for its formation. Whether the haustorium requires any plant signals during its development is unknown, but the fact that haustoria formed in itro do not mature (Heath, 1989) suggests that such signals might exist. Because the forma ...
... because of the inter-organismal co-operation that must be required for its formation. Whether the haustorium requires any plant signals during its development is unknown, but the fact that haustoria formed in itro do not mature (Heath, 1989) suggests that such signals might exist. Because the forma ...
Antimicrobial, Antitumor and Phytochemical screening for the crude
... Moringa oleifera Lamk. is a member of the Moringaceae family. It originated in India, Srilanka and can be grown up in Asia Minor and Africa as well. Moringa oleifera, commonly referred to simply as Moringa, is the most widely cultivated variety of the genus Moringa. It is an exceptionally nutritious ...
... Moringa oleifera Lamk. is a member of the Moringaceae family. It originated in India, Srilanka and can be grown up in Asia Minor and Africa as well. Moringa oleifera, commonly referred to simply as Moringa, is the most widely cultivated variety of the genus Moringa. It is an exceptionally nutritious ...
Glucose-dependent, CAMP-mediated ATP efflux from
... P H 0 3 genes. Preliminary experiments verified that YMR4 cells do not contain measurable extracellular ATPase activity while cells containing either acid phosphatase gene were able to hydrolyse extracellular ATP (unpublished observations). Fig. 1 shows the time course of the efflux of ATP and ADP ( ...
... P H 0 3 genes. Preliminary experiments verified that YMR4 cells do not contain measurable extracellular ATPase activity while cells containing either acid phosphatase gene were able to hydrolyse extracellular ATP (unpublished observations). Fig. 1 shows the time course of the efflux of ATP and ADP ( ...
Full-Text PDF
... of the oxidized form of the human tetherin ectodomain, this N-terminal region is unresolved in the crystal, further suggesting conformational flexibility in this area [22]. Tetherin is located both at the plasma membrane and in intracellular compartments. At the plasma membrane, the GPI anchor embed ...
... of the oxidized form of the human tetherin ectodomain, this N-terminal region is unresolved in the crystal, further suggesting conformational flexibility in this area [22]. Tetherin is located both at the plasma membrane and in intracellular compartments. At the plasma membrane, the GPI anchor embed ...
Microbiology
... determinant for class 2 mAbs. Firstly, mAb binding to a 100 kDa protein on Western blots of a 2-D fingerprint of outer-membrane proteins. This protein was identified as the major constituent of a polar flagellum preparation by SDS-PAGE. Secondly, lack of mAb binding to the mutant A . brasilense Sp7 ...
... determinant for class 2 mAbs. Firstly, mAb binding to a 100 kDa protein on Western blots of a 2-D fingerprint of outer-membrane proteins. This protein was identified as the major constituent of a polar flagellum preparation by SDS-PAGE. Secondly, lack of mAb binding to the mutant A . brasilense Sp7 ...
Soluble factors from human endometrium promote angiogenesis
... to affect EC. Disaggregated monolayer cultures were chosen in preference to explant cultures to maintain optimal oxygenation and nutrition for all cells, and to allow visible assessment of endometrial cell viability. After 24 h in culture the endometrial monolayers were washed twice in medium in ord ...
... to affect EC. Disaggregated monolayer cultures were chosen in preference to explant cultures to maintain optimal oxygenation and nutrition for all cells, and to allow visible assessment of endometrial cell viability. After 24 h in culture the endometrial monolayers were washed twice in medium in ord ...
Somite dorsoventral polarity - Development
... that indicated that sclerotome determination occurs relatively late in development (Dockter and Ordahl, 1998) because sclerotome fragments from somites much older than stage III consistently gave rise to muscle and dermis after being translocated to a dorsal position. Interestingly, such implants al ...
... that indicated that sclerotome determination occurs relatively late in development (Dockter and Ordahl, 1998) because sclerotome fragments from somites much older than stage III consistently gave rise to muscle and dermis after being translocated to a dorsal position. Interestingly, such implants al ...
PDF
... in Hoxa3 −/− mutants (Fig. 2O,P). By E11.5, Gcm2 expression was undetectable (Fig. 2Q-T). A small group of Gcm2 Foxn1 doublenegative cells that was attached to the thymus persisted until E12.5 (Fig. 2D,F,R,T), but was undetectable by E13.0 (Fig. 3H,I). These results are consistent with previous repo ...
... in Hoxa3 −/− mutants (Fig. 2O,P). By E11.5, Gcm2 expression was undetectable (Fig. 2Q-T). A small group of Gcm2 Foxn1 doublenegative cells that was attached to the thymus persisted until E12.5 (Fig. 2D,F,R,T), but was undetectable by E13.0 (Fig. 3H,I). These results are consistent with previous repo ...
Biology I Pacing Guide
... Draw conclusions about how organisms are classified into a hierarchy of groups and subgroups based on similarities that reflect their evolutionary relationships. (DOK 2) Characteristics of the six kingdoms ...
... Draw conclusions about how organisms are classified into a hierarchy of groups and subgroups based on similarities that reflect their evolutionary relationships. (DOK 2) Characteristics of the six kingdoms ...
Significance of hydrolytic enzymes expressed during xylem
... self-destruction. It has a central role in development, tissue homeostasis and integrity, but also during immune or defence responses of all multicellular organisms. But PCD has even been observed in protozoa and is generally considered to be an evolutionary conserved mechanism (Ameisen 2002). In co ...
... self-destruction. It has a central role in development, tissue homeostasis and integrity, but also during immune or defence responses of all multicellular organisms. But PCD has even been observed in protozoa and is generally considered to be an evolutionary conserved mechanism (Ameisen 2002). In co ...
Nedd8 processing enzymes inSchizosaccharomyces pombe
... differences in Nedd8 substrates between these organisms. Just as ubiquitylation is reversed by ubiquitin isopeptidases, Nedd8 conjugation is reversed by Nedd8 isopeptidases in a process known as deneddylation. The best characterized deneddylating enzymes include the COP9 signalosome subunit Csn5 [11 ...
... differences in Nedd8 substrates between these organisms. Just as ubiquitylation is reversed by ubiquitin isopeptidases, Nedd8 conjugation is reversed by Nedd8 isopeptidases in a process known as deneddylation. The best characterized deneddylating enzymes include the COP9 signalosome subunit Csn5 [11 ...
Fine mapping of re-arranged Y chromosome in three infertile
... (named thereafter ‘centromeric probe’) (Vysis, IL, USA). On metaphase chromosomes of control fertile patients, all three probes displayed a strong spot-like signal on each chromatid, which localized exclusively to the proximal part of the long arm of the Y chromosome, whereas, as expected, no signal ...
... (named thereafter ‘centromeric probe’) (Vysis, IL, USA). On metaphase chromosomes of control fertile patients, all three probes displayed a strong spot-like signal on each chromatid, which localized exclusively to the proximal part of the long arm of the Y chromosome, whereas, as expected, no signal ...
Synthesis and characterization of poly-β-hydroxybutyrate
... in the presence of ethanol and lactic acid. Dry cell mass in the presence of monomer β–hydroxybutyric acid (0.5%w/v) was 1.5g/L. PHB accumulation in the presence of table sugar, molasses and fructose was 28.23%, 23.06% and 19.76% (0.57; 0.72 and 0.34 g/L of media), respectively. PHB accumulation in ...
... in the presence of ethanol and lactic acid. Dry cell mass in the presence of monomer β–hydroxybutyric acid (0.5%w/v) was 1.5g/L. PHB accumulation in the presence of table sugar, molasses and fructose was 28.23%, 23.06% and 19.76% (0.57; 0.72 and 0.34 g/L of media), respectively. PHB accumulation in ...
Relating the mechanics of the primary plant cell
... only confines the growth behavior of the individual cell but also regulates the development of the entire tissue, since neighboring cells are tightly connected at their shared walls. Generally, plant cells do not detach, slide, or move against each other, and the architecture of a plant tissue is th ...
... only confines the growth behavior of the individual cell but also regulates the development of the entire tissue, since neighboring cells are tightly connected at their shared walls. Generally, plant cells do not detach, slide, or move against each other, and the architecture of a plant tissue is th ...
Utilization of Amino Acids and Lack of Diazotrophy in
... Growth on amino acids as nitrogen sources by nonsulphur purple bacteria (Gest & Kamen, 1960; Yoch, 1978) and green sulphur bacteria (Heda & Madigan, 1986) is associated with nitrogenase derepression. Therefore, strains of Chlorofexus were grown in similar fashion and tested for acetylene reduction. ...
... Growth on amino acids as nitrogen sources by nonsulphur purple bacteria (Gest & Kamen, 1960; Yoch, 1978) and green sulphur bacteria (Heda & Madigan, 1986) is associated with nitrogenase derepression. Therefore, strains of Chlorofexus were grown in similar fashion and tested for acetylene reduction. ...
Biology I
... and precision in using graduated cylinders, balances, beakers, thermometers, and rulers *1b. Formulate questions that can be answered through research and experimental design. (DOK 3) *1c. Apply the components of scientific processes and methods in classroom and laboratory investigations (e.g., hypo ...
... and precision in using graduated cylinders, balances, beakers, thermometers, and rulers *1b. Formulate questions that can be answered through research and experimental design. (DOK 3) *1c. Apply the components of scientific processes and methods in classroom and laboratory investigations (e.g., hypo ...
Association of CAD, a multifunctional protein involved in pyrimidine
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
Anatomy & Physiology Workbook For Dummies
... Janet Rae-Dupree has been covering science and technology in Silicon Valley since 1993 for a number of publications, including U.S. News & World Report, BusinessWeek, the San Jose Mercury News, and the Silicon Valley/San Jose Business Journal. She was a frequent guest on cable channel Tech TV’s “Sil ...
... Janet Rae-Dupree has been covering science and technology in Silicon Valley since 1993 for a number of publications, including U.S. News & World Report, BusinessWeek, the San Jose Mercury News, and the Silicon Valley/San Jose Business Journal. She was a frequent guest on cable channel Tech TV’s “Sil ...