Characteristics, causes and evolutionary consequences of male
... evidence of divergence rate heterogeneity in different genomic regions. One facet of mutation rate variation is the propensity for genetic change to correlate with the number of germ cell divisions, reflecting the replication-dependent origin of many mutations. Haldane was the first to connect this ...
... evidence of divergence rate heterogeneity in different genomic regions. One facet of mutation rate variation is the propensity for genetic change to correlate with the number of germ cell divisions, reflecting the replication-dependent origin of many mutations. Haldane was the first to connect this ...
pcr (polymerase chain reaction)
... 4. Add the following reaction volumes from the table to each of the three 1.5 mL tubes. The positive control is a control insert and control vector that comes with the In-Fusion Kit that will tell you whether you did the reaction correctly after transformation (see below). The negative control has o ...
... 4. Add the following reaction volumes from the table to each of the three 1.5 mL tubes. The positive control is a control insert and control vector that comes with the In-Fusion Kit that will tell you whether you did the reaction correctly after transformation (see below). The negative control has o ...
Practical: Ranges
... strand returns the strand information in a compact representation called a run-length encoding. The ‘names’ could have been specified when the instance was constructed; once named, the GRanges instance can be subset by name like a regular vector. As the GRanges function suggests, the GRanges class e ...
... strand returns the strand information in a compact representation called a run-length encoding. The ‘names’ could have been specified when the instance was constructed; once named, the GRanges instance can be subset by name like a regular vector. As the GRanges function suggests, the GRanges class e ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
... both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentration of the active S-adenosyl methionine is important factor that strongly influenc ...
Understanding Lynch Syndrome - Hereditary Nonpolyposis
... whose tumour was tested. The goal of this test is to try to find a specific mutation in one of the Lynch syndrome genes. It is possible that index genetic testing may not identify a specific mutation. In such cases, it is still possible that a gene mutation is being passed down in the family, but it ...
... whose tumour was tested. The goal of this test is to try to find a specific mutation in one of the Lynch syndrome genes. It is possible that index genetic testing may not identify a specific mutation. In such cases, it is still possible that a gene mutation is being passed down in the family, but it ...
DNA SEQUENCING (using a Li
... If the fragment is short, and the sequence desired is close to the 5' end of that fragment, then the run time will be short (1-2 h). If the fragment is long, and the desired sequence is closer to the 3' end, then the run time will be longer (2-5 h). The other method of sequencing DNA (Sanger 1977) u ...
... If the fragment is short, and the sequence desired is close to the 5' end of that fragment, then the run time will be short (1-2 h). If the fragment is long, and the desired sequence is closer to the 3' end, then the run time will be longer (2-5 h). The other method of sequencing DNA (Sanger 1977) u ...
Basic reading, writing and informatics skills for biomedical research
... The morphological properties of each word/phrase, e.g., what type of plural does it take, what type of past tense, how does it combine with affixes like "re" and "ation"; How to disambiguate word senses - This allows Cognition's technology to pick the correct word meaning of ambiguous words in conte ...
... The morphological properties of each word/phrase, e.g., what type of plural does it take, what type of past tense, how does it combine with affixes like "re" and "ation"; How to disambiguate word senses - This allows Cognition's technology to pick the correct word meaning of ambiguous words in conte ...
Import, distribution and retail of gm
... carnation is cultivated since decades, it has never been found growing in the wild. The introduced traits (modified flower color and herbicide tolerance) do not introduce a potential for weediness. Formation of seed on cut flowers is highly improbable. Carnation is pollinated exclusively by butterfl ...
... carnation is cultivated since decades, it has never been found growing in the wild. The introduced traits (modified flower color and herbicide tolerance) do not introduce a potential for weediness. Formation of seed on cut flowers is highly improbable. Carnation is pollinated exclusively by butterfl ...
Detection of Mosaicism by Augmented Exome
... hypotonia, intellectual disability, speech delay, and a characteristic pattern of dysmorphic facial features. These individuals represent the 6th and 7th reported cases of GATAD2B-associated intellectual disability and further characterize the molecular mechanism and phenotypic spectrum of this diso ...
... hypotonia, intellectual disability, speech delay, and a characteristic pattern of dysmorphic facial features. These individuals represent the 6th and 7th reported cases of GATAD2B-associated intellectual disability and further characterize the molecular mechanism and phenotypic spectrum of this diso ...
Investigating the importance of anatomical homology for
... bioRxiv preprint first posted online Oct. 5, 2015; doi: http://dx.doi.org/10.1101/028449. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... bioRxiv preprint first posted online Oct. 5, 2015; doi: http://dx.doi.org/10.1101/028449. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
SNP-Based Mapping of Crossover Recombination in
... of morphological phenotypes in C. elegans) . As a result, each experiment typically measures crossover frequency within a sin gle interval, which prevents detection of chromosomes with mul tiple crossovers and complicates determination of crossover dis tribution along chromosomes. In addition, so ...
... of morphological phenotypes in C. elegans) . As a result, each experiment typically measures crossover frequency within a sin gle interval, which prevents detection of chromosomes with mul tiple crossovers and complicates determination of crossover dis tribution along chromosomes. In addition, so ...
Supplementary Table 1 Entrez Gene (http://www.ncbi.nlm.nih.gov
... The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought ...
... The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought ...
Sporulation in BhciZZus subtilis 168. Control of
... common structural gene for the enzyme may be regulated by two distinct control mechanisms, one of which is associated with phosphate starvation while the other is specific to the sporulation process. In support of this suggestion, Glenn (1971) isolated a number of mutants of Bacillus subtilis 168 wh ...
... common structural gene for the enzyme may be regulated by two distinct control mechanisms, one of which is associated with phosphate starvation while the other is specific to the sporulation process. In support of this suggestion, Glenn (1971) isolated a number of mutants of Bacillus subtilis 168 wh ...
Intracellular killing of bacteria: is Dictyostelium a model macrophage
... Kypri et al., 2007). As seen for wash KO cells, lvsB KO cells also have specific growth defects: they are unable to feed upon M. luteus and some pathogenic Klebsiella strains, but can grow as efficiently as WT cells on other bacteria, as Bacillus and E. coli. Overall, the general conclusion of these ...
... Kypri et al., 2007). As seen for wash KO cells, lvsB KO cells also have specific growth defects: they are unable to feed upon M. luteus and some pathogenic Klebsiella strains, but can grow as efficiently as WT cells on other bacteria, as Bacillus and E. coli. Overall, the general conclusion of these ...
DNA breathing dynamics distinguish binding from nonbinding
... A large (800 bp) PLG promoter fragment has been isolated by YY1 ChIP and the sequence is available in the BIOBASE database (http://www.biobase-international.com). The exact YY1 binding position within the fragment, however, is not known. By searching for YY1 consensus sequences in the BIOBASE fragme ...
... A large (800 bp) PLG promoter fragment has been isolated by YY1 ChIP and the sequence is available in the BIOBASE database (http://www.biobase-international.com). The exact YY1 binding position within the fragment, however, is not known. By searching for YY1 consensus sequences in the BIOBASE fragme ...
About the origins of Molecular Biology and of our Department at UTD
... the development of blood vessel patterns in chick embryos by means of measuring redox (reduction-oxidation) potentials and pH gradients that might precede the differentiation of blood vessels. To do so, I needed micro redox and pH electrodes. To learn how to make them and measure those parameters, ...
... the development of blood vessel patterns in chick embryos by means of measuring redox (reduction-oxidation) potentials and pH gradients that might precede the differentiation of blood vessels. To do so, I needed micro redox and pH electrodes. To learn how to make them and measure those parameters, ...
The Co-Evolution of Genes and Culture Pedigrees and the
... lactase switch in several individuals. They found many differences. For example, at one particular position in the sequence of DNA, some individuals might have an adenine (A) nucleotide and others a thymine (T) nucleotide. Most of the differences in DNA sequence have no effect on lactase production. ...
... lactase switch in several individuals. They found many differences. For example, at one particular position in the sequence of DNA, some individuals might have an adenine (A) nucleotide and others a thymine (T) nucleotide. Most of the differences in DNA sequence have no effect on lactase production. ...
Development and implementation of genomic predictions in beef cattle
... 4,282 animals in Blonde d’Aquitaine. Of these animals, 8 to 35% were well proven progeny-tested bulls. ...
... 4,282 animals in Blonde d’Aquitaine. Of these animals, 8 to 35% were well proven progeny-tested bulls. ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
... 1) Towards transgenic engineering of late-onset, male-specific double muscling (P008) Dimitri Pirottin, Luc Grobet, and Michel Georges (University of Liège, Belgium) ...
... 1) Towards transgenic engineering of late-onset, male-specific double muscling (P008) Dimitri Pirottin, Luc Grobet, and Michel Georges (University of Liège, Belgium) ...
Resistance genes in barley - Journal of Applied Genetics
... like the nucleotide-binding site (NBS) and leucine-rich repeats (LRR). An interesting feature of this class of R genes is that they are involved in gene-for-gene resistance towards either fungal, viral, bacterial or nematode disease resistance. The conservation between different NBS-LRR resistance g ...
... like the nucleotide-binding site (NBS) and leucine-rich repeats (LRR). An interesting feature of this class of R genes is that they are involved in gene-for-gene resistance towards either fungal, viral, bacterial or nematode disease resistance. The conservation between different NBS-LRR resistance g ...
Efficient Virus-Induced Gene Silencing in Roots
... was also clearly visible in N. benthamiana roots as previously described (MacFarlane and Popovich, 2000). Nevertheless, a higher percentage of infected plants expressed GFP in roots at 7 d postinoculation (dpi; Fig. 1b, ii) in plants inoculated with the TRV-2b vector (55%) compared with the TRV-D2b- ...
... was also clearly visible in N. benthamiana roots as previously described (MacFarlane and Popovich, 2000). Nevertheless, a higher percentage of infected plants expressed GFP in roots at 7 d postinoculation (dpi; Fig. 1b, ii) in plants inoculated with the TRV-2b vector (55%) compared with the TRV-D2b- ...
CHAPTER 17 Variation in Chromosomal Number and Structure
... carrier females have 55–200 copies, but do not show symptoms. iii. Individuals with fragile X syndrome have 200–1,300 copies, indicating that tandem amplification of this sequence is tolerated until a threshold number of copies is reached. iv. Amplification of CGG repeats occurs only in females, per ...
... carrier females have 55–200 copies, but do not show symptoms. iii. Individuals with fragile X syndrome have 200–1,300 copies, indicating that tandem amplification of this sequence is tolerated until a threshold number of copies is reached. iv. Amplification of CGG repeats occurs only in females, per ...
A DNA-sequence based phylogeny for triculine snails (Gastropoda
... The habitat requirements of these taxa, which appear to preserve past biogeographical patterns, have led to their use in historical biogeographical studies of not only the Rissooidea but also of Schistosoma (see Davis, 1979, 1992; Attwood, 2001; Attwood & Johnston, 2001; Attwood et al., 2002b). The ...
... The habitat requirements of these taxa, which appear to preserve past biogeographical patterns, have led to their use in historical biogeographical studies of not only the Rissooidea but also of Schistosoma (see Davis, 1979, 1992; Attwood, 2001; Attwood & Johnston, 2001; Attwood et al., 2002b). The ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse