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The CHARGE Targeted Sequencing Study
The CHARGE Targeted Sequencing Study

... gene transcription were studied using dual luciferase assay experiments. Chromatin immunoprecipitation studies completed by the ENCODE project show that chr11:47227430 is located in a 280bp region of active chromatin in HepG2 cells that binds FOXA1.33 This 280-bp DNA fragment (containing the referen ...
Smiley Face Genetics
Smiley Face Genetics

... Genetics with a Smile Purpose: To distinguish between dominant and recessive traits ...
Imunocytochemistry Detection of M1CA Gene Receptor in Human P
Imunocytochemistry Detection of M1CA Gene Receptor in Human P

... macrophages, which are the major effectors facilitating killing and inhibiting replication of Brucella. The Th1 cell induced - cytokines, like IFN- γ, are important for the ctivation of macrophages and in resistance to in vivo and in vitro Brucella infections (Zhan et al., 1993)) . gamma interferon ...
Nature Genetics: doi:10.1038/ng.3304
Nature Genetics: doi:10.1038/ng.3304

... proliferation and differentiation of erythrocyte progenitor cells. In secondary erythropoiesis, the increased red cell production is driven by external factors (e.g. hypoxia or defects in oxygen sensing) through increased erythropoietin production, and patients typically have high or inappropriately ...
Examination of G72 and D-amino-acid oxidase
Examination of G72 and D-amino-acid oxidase

... Genomics). Genotype frequencies for cases and controls were in Hardy–Weinberg equilibrium. Singlemarker and haplotype-based analyses were performed. For the single-marker analysis, we used the Armitage trend test (allelewise analysis) and the standard w2 test (genotypewise analysis). For the compari ...
How to use KAIKObase Version 3.1.0
How to use KAIKObase Version 3.1.0

... KAIKObase is an integrated silkworm genome browser with 3 map browsers, 1 gene viewer, and 5 independent databases. * PGmap : physical map and genetic linkage map for each chromosome. * UnifiedMap : PGmap containing genome contigs, BAC-ends, fosmid-ends, and Fingerprint Contigs (FPC). * GBrowse : ge ...
APPLYING THE
APPLYING THE

... color, size, etc. Plants can be tall or short, have red, yellow or white flowers, etc. A guinea pig can have white or brown fur which can be long, short or curly. All of these TRAITS express how the individual looks or appears to you. GENETICS is the study of how these hereditary traits are passed o ...
beautiful bicolours - tuxedo and magpie cats
beautiful bicolours - tuxedo and magpie cats

... exactly as though a piece of coloured area has broken off and migrated elsewhere. Spots could end up almost anywhere depending on the timing of cracking, the size of the coloured domains, and the trajectories they take over the embryonic surface as it grows. Terada and Watanabe found it possible to ...
Radiation Hybrid Mapping: A Somatic Cell Genetic Method for
Radiation Hybrid Mapping: A Somatic Cell Genetic Method for

... to probes Eg, which recognizes locus S16, and SFI05, which recognizes Lira. locus $48. Because Sma I, Xho I, and Cla I do not cleave DNA that is methylated at their respective sites, partial methylation in genomic blood cell DNA results in partial digestion with these enzymes and leads to multiple f ...
Caenorhabditis elegans chromosome arms are anchored to the
Caenorhabditis elegans chromosome arms are anchored to the

... Background: Although Caenorhabditis elegans was the first multicellular organism with a completely sequenced genome, how this genome is arranged within the nucleus is not known. Results: We determined the genomic regions associated with the nuclear transmembrane protein LEM-2 in mixedstage C. elegan ...
Genes, Environment and Sport Performance
Genes, Environment and Sport Performance

... considerable advancement in the methods of examination, it is unlikely they will be resolved soon. This is not to say that understanding of genetic and environmental influence has not advanced. It certainly has; moreover, these advances have obvious importance for understanding the development of ex ...
Comparative Bacterial Genomics Genome Databases
Comparative Bacterial Genomics Genome Databases

... similar information. The next part is the annotations, genes and CDS (CoDing Sequences). In this section the genes are described by their location, direction, note, and translation. ...
Redalyc.Prevalence of ΔF508 mutation in the cystic fibrosis
Redalyc.Prevalence of ΔF508 mutation in the cystic fibrosis

... this study is a worldwide spread exam that, combined with ...
—1— User Guide © Copyright 2009 Robert C. Edgar, all rights
—1— User Guide © Copyright 2009 Robert C. Edgar, all rights

... substitutions, insertions, deletions, moves, copies and so on. This division is driven by software engineering rather than biological considerations: it is currently possible to simulate evolution of a single chromosome on a commodity computer typically found in a compute cluster, but an entire geno ...
Differentiation of plant cells during symbiotic nitrogen fixation
Differentiation of plant cells during symbiotic nitrogen fixation

... case of indeterminate nodules, such as the nodules formed by pea or Medicago truncatula, the nodule meristem differentiates from inner-cortical cells and is persistent, with new cells being constantly produced and infected. In determinate nodules, such as those of soybean or Lotus japonicus, the nod ...
the Liver - Saudi Medical Journal
the Liver - Saudi Medical Journal

... We have used these strategies to examine associations between maternal carriage of cytokine polymorphisms and RSA by investigating certain genetic polymorphism in the promoter region of TNF-α, IL-10 and IL-6 gene in Saudi females. Tumor necrosis factor (TNFα) is a potent cytokine with a wide range o ...
Transition bias and substitution models
Transition bias and substitution models

... I often say that when you can measure what you are speaking about, and express it in numbers, you know something about it; but when you cannot measure it, when you cannot express it in numbers, your knowledge is of a meagre and unsatisfactory kind; it may be the beginning of knowledge, but you have ...
Resources: - Real Science
Resources: - Real Science

... Pupils will not know some of the words used in the text. Meanings are given below, followed by an exercise in matching words and meanings. Teachers may choose to provide some or all of the meanings to help pupils read and understand the story. An approach that leads to better learning is to ask pupi ...
Involvement of HLS1 in Sugar and Auxin
Involvement of HLS1 in Sugar and Auxin

... Borisjuk et al. 2004, Gibson 2005, Wingler et al. 2006). Studies carried out over the past 10 years have begun to reveal the molecular mechanisms underlying sugar signaling in higher plants. Genetic screens for Arabidopsis mutants have provided powerful tools to identify genes involved in sugar sign ...
Possible consequences of the overlap between the CaMV 35S
Possible consequences of the overlap between the CaMV 35S

... survey shows that short versions of the P35S (up to position -522 relative to the CAP) overlap only with domain D4 of P6. This domain, when mutated, deleted, or inverted, reduces the rate of viral movement and influences viral host range.10,29-31 Thus, the D4 domain appears to be partially dispensab ...
A machine learning approach to gene expression data analysis
A machine learning approach to gene expression data analysis

... Effectiveness of the RS method and other open problems 1. Can we explain the effectiveness of RS through the diversity of the base learners ? 2. Can we get a bias-variance interpretation ? 3. Can we get quantitative relationships between dimensionality reduction, redundant features and correlation ...
Stabilizing synthetic data in the DNA of living organisms
Stabilizing synthetic data in the DNA of living organisms

... sequences. Thus, it is extremely rare for the occurrence of both sources of errors at the same position within the multiple data-encoded regions. When the letters at a same position of multiple alignments of decoded data sequences are different, it is likely that the minority letters have arisen fro ...
Principles of Genetics Class Schedule
Principles of Genetics Class Schedule

... • What were Mendel’s critical insights that enabled him to unravel the general rules of inheritance? • Why were the insights so important? • Are all individuals with the same phenotype equivalent with respect to transmission of genetic information? • How can one differentiate between different types ...
Liu - Blumberg Lab
Liu - Blumberg Lab

... cellular context • Identified variation in transcriptional networks across cell lines under the same conditions • Generated general prediction factors for lncRNA hits using machine learning ...
DNA Tribes Digest for October 28, 2010
DNA Tribes Digest for October 28, 2010

... Once your testing is complete, we offer several options to keep your report current and to customize your genetic analysis for the information you want. (Prices are listed as of October 30, 2010 and are subject to change.) Updating Your Analysis: DNA Tribes® analysis is updated on a periodic basis t ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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