ABO Antibodies
... When exposed to Group A or B antigens (or both) Group O persons will have an immune response that results in the production of separate immune anti-A and/or anti-B antibodies. This could be seen in a fetomaternal bleed of a Group O mom with a Group A baby. (Hemolytic Disease of the Newborn) ...
... When exposed to Group A or B antigens (or both) Group O persons will have an immune response that results in the production of separate immune anti-A and/or anti-B antibodies. This could be seen in a fetomaternal bleed of a Group O mom with a Group A baby. (Hemolytic Disease of the Newborn) ...
Endocytobiosis and Cell Research
... Bradyrhizobium japonicum and Sinorhizobium fredii are sensitive towards the phytoalexin glyceollin. The antibacterial effect of the soybean phytoalexin glyceollin was assayed using a liquid microculture technique (Parniske et al., 1991). Log-phase cells of Bradyrhizobium japonicum and Sinorhizobium ...
... Bradyrhizobium japonicum and Sinorhizobium fredii are sensitive towards the phytoalexin glyceollin. The antibacterial effect of the soybean phytoalexin glyceollin was assayed using a liquid microculture technique (Parniske et al., 1991). Log-phase cells of Bradyrhizobium japonicum and Sinorhizobium ...
Alu Human Polymorphism
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
... inherited from each parent – Most occurred millions of years ago and are often on both pairs of chromosomes – There are Alu elements that have occurred since humans branched from other primates – This gives rise to dimorphic Alus from the last hundreds of thousands of years – Estimated to jump 1:200 ...
Fully automated pipeline for detection of sex linked genes using
... all mother reads were assembled into contigs with Trinity assembler [11]. These contigs therefore represented the reference. This is a crucial step of the procedure since in previous works [12,13], the reference was derived from all individuals. Including all sequences from all individuals for build ...
... all mother reads were assembled into contigs with Trinity assembler [11]. These contigs therefore represented the reference. This is a crucial step of the procedure since in previous works [12,13], the reference was derived from all individuals. Including all sequences from all individuals for build ...
Slide 1
... (C) The distribution across markers of the proportion of H. petiolaris alleles seen in experimental hybrids. There were three generations of crossing within the hybrid population, followed by two generations of backcrossing to H. annuus. Therefore, in the absence of selection, one expects 1/8 of t ...
... (C) The distribution across markers of the proportion of H. petiolaris alleles seen in experimental hybrids. There were three generations of crossing within the hybrid population, followed by two generations of backcrossing to H. annuus. Therefore, in the absence of selection, one expects 1/8 of t ...
Peer Review 1
... Targeted gene knock-in would be used as opposed to random integration with a transgenic construct in order to prevent side effects due to disruption of essential genomic sequences. Thus, a construct would be chosen that targets a region of the genome with no known function. The construct would recom ...
... Targeted gene knock-in would be used as opposed to random integration with a transgenic construct in order to prevent side effects due to disruption of essential genomic sequences. Thus, a construct would be chosen that targets a region of the genome with no known function. The construct would recom ...
The Chloroplast trnT–trnF Region in the Seed Plant
... with its flanking genes (Fig. 1A), it forms a cistron that is conserved from mosses to seed plants (Taberlet et al. 1991). Even where chloroplast genomes have undergone extreme structural changes, such as in Pinus thunbergii (Wakasugi et al. 1994) (Fig. 1B), which has pseudogenized ndhF genes located ...
... with its flanking genes (Fig. 1A), it forms a cistron that is conserved from mosses to seed plants (Taberlet et al. 1991). Even where chloroplast genomes have undergone extreme structural changes, such as in Pinus thunbergii (Wakasugi et al. 1994) (Fig. 1B), which has pseudogenized ndhF genes located ...
Minireview Shifty Ciliates: Frequent Programmed
... synthesis into pol, producing a Gag-Pol fusion protein. The frequency of this frameshift is as much as 10,000fold greater than the estimated rate of spontaneous translational frameshifting. The sequence of the region in and around the site of frameshifting stimulates this impressive increase in “err ...
... synthesis into pol, producing a Gag-Pol fusion protein. The frequency of this frameshift is as much as 10,000fold greater than the estimated rate of spontaneous translational frameshifting. The sequence of the region in and around the site of frameshifting stimulates this impressive increase in “err ...
Alteration of Gene Expression by Chromosome Loss in the Postnatal
... flow sorting, cells were harvested by centrifugation for 10 min at 500 ⫻ g, resuspended in 50 –100 l of Opti-Mem, and plated onto 12 mm coverslips (Fisher Scientific, Pittsburgh, PA) coated with cell-tak (Collaborative Research, Bedford, MA) or poly-lysine (Sigma) as described previously (Fukushima ...
... flow sorting, cells were harvested by centrifugation for 10 min at 500 ⫻ g, resuspended in 50 –100 l of Opti-Mem, and plated onto 12 mm coverslips (Fisher Scientific, Pittsburgh, PA) coated with cell-tak (Collaborative Research, Bedford, MA) or poly-lysine (Sigma) as described previously (Fukushima ...
doc
... If you have an emergency and cannot submit a quiz in person, email it in by the start of class on the due date. If you do so, first remove the instructions and extras (blank lines, alternative answers for multiple choice questions) from your document, so that only your answers, a minimal amount whit ...
... If you have an emergency and cannot submit a quiz in person, email it in by the start of class on the due date. If you do so, first remove the instructions and extras (blank lines, alternative answers for multiple choice questions) from your document, so that only your answers, a minimal amount whit ...
fall bellwork
... C. The mutation causes damage when it occurs. D. The mutation creates entirely new organisms. ...
... C. The mutation causes damage when it occurs. D. The mutation creates entirely new organisms. ...
Invited Review: How sleep deprivation affects gene expression in
... Thus Glut1 induction may represent another mechanism by which the brain responds to the increased energy requirements of the waking state. Several heat shock proteins and molecular chaperones such as HSP60, HSP70, and BiP show higher mRNA levels after 8 h of wakefulness. BiP, the major chaperone of ...
... Thus Glut1 induction may represent another mechanism by which the brain responds to the increased energy requirements of the waking state. Several heat shock proteins and molecular chaperones such as HSP60, HSP70, and BiP show higher mRNA levels after 8 h of wakefulness. BiP, the major chaperone of ...
(type I) and mannose-resistant F8 (P) fimbriae of Escherichia coli
... O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. In contrast, P-fimbrial determinants may be located in different regions on the chromosomes of extra-intestinal E. coli isolates. Very often the genet ...
... O18:K5. Therefore, it seems that the gene clusters coding for type I fimbriae are located at fixed positions on the chromosomes of different strains. In contrast, P-fimbrial determinants may be located in different regions on the chromosomes of extra-intestinal E. coli isolates. Very often the genet ...
POCUS: mining genomic sequence annotation to predict disease
... functional annotation A list of 29 Online Mendelian Inheritance in Man (OMIM) diseases was compiled for which three or more contributing genes were known (and were also present within Ensembl), and the degree to which genes for the same disease shared InterPro domain and GO identifiers (IDs) was ass ...
... functional annotation A list of 29 Online Mendelian Inheritance in Man (OMIM) diseases was compiled for which three or more contributing genes were known (and were also present within Ensembl), and the degree to which genes for the same disease shared InterPro domain and GO identifiers (IDs) was ass ...
objectives
... 34. Distinguish between parental and recombinant phenotypes 35. Explain how crossing over can unlink genes 36. Map a linear sequence of genes on a chromosome using given recombination frequencies from experimental crosses 37. Explain what additional information cytological maps provide over crossove ...
... 34. Distinguish between parental and recombinant phenotypes 35. Explain how crossing over can unlink genes 36. Map a linear sequence of genes on a chromosome using given recombination frequencies from experimental crosses 37. Explain what additional information cytological maps provide over crossove ...
Convergent evolution of complex regulatory
... and B). However, although the profiles shared many interactions, they also displayed differences with contacts observed in digits but not in GT and vice versa (Fig. 2C). Hoxd13 interaction peaks were aligned with both evolutionary conserved noncoding sequences and the GT H3K27 acetylation profile (f ...
... and B). However, although the profiles shared many interactions, they also displayed differences with contacts observed in digits but not in GT and vice versa (Fig. 2C). Hoxd13 interaction peaks were aligned with both evolutionary conserved noncoding sequences and the GT H3K27 acetylation profile (f ...
mutation and Y486D mutation in exon 5 of the UGT1A1 - Funpec-RP
... level of 1-6 mg/dL (Bosma et al., 1995; Bayram et al., 2013). CN is related to inborn errors in metabolism and are inherited as autosomal recessive traits. These traits result from unconjugated hyperbilirubinemia because of defective bilirubin uridinediphosphate (UDP)glucuronosyltransferase (UGT1A1) ...
... level of 1-6 mg/dL (Bosma et al., 1995; Bayram et al., 2013). CN is related to inborn errors in metabolism and are inherited as autosomal recessive traits. These traits result from unconjugated hyperbilirubinemia because of defective bilirubin uridinediphosphate (UDP)glucuronosyltransferase (UGT1A1) ...
pdf
... Diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. Nitrite reductase genes were PCR-amplified with degenerate primers (specific to ...
... Diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. Nitrite reductase genes were PCR-amplified with degenerate primers (specific to ...
P57: Beckwith-Wiedemann Syndrome
... Inherited in autosomal dominant fashion Mapped to chromosome 11p15 Translocation breakpoints found within chromosome map to three distinct regions Region 1, BWSCR1, contains 5 translocation breakpoints, which all disrupt the KCNQ1 gene and is the region of primary concern ...
... Inherited in autosomal dominant fashion Mapped to chromosome 11p15 Translocation breakpoints found within chromosome map to three distinct regions Region 1, BWSCR1, contains 5 translocation breakpoints, which all disrupt the KCNQ1 gene and is the region of primary concern ...
Nucleotide Sequence of the Osmoregulatory proU Operon of
... FIG. 1. Strategy for DNA sequence determination of proU. The map of insert DNA of pHYD58 (20) is shown, and relevailt restriction sites are marked; a kilobase scale is included. The insert includes 5 kb of DNA clockwise of the BgIII site from the E. coli proU iocus (thin line) and 1 kb of Mu c DNA ( ...
... FIG. 1. Strategy for DNA sequence determination of proU. The map of insert DNA of pHYD58 (20) is shown, and relevailt restriction sites are marked; a kilobase scale is included. The insert includes 5 kb of DNA clockwise of the BgIII site from the E. coli proU iocus (thin line) and 1 kb of Mu c DNA ( ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
... probes and containing an insert of 14 .5 kb, termed XGSB 16.1, was restriction mapped (Fig. 1 B) . The Pvu II-Bam HI fragment, GB2PE, which contained neither human repeats nor CRI-1- and CRI-4-like sequences, hybridized to the 14.5-kb Bam HI fragment characteristic ofan S allele and to nonallelic fr ...
HTR1A a Novel Type 1 Diabetes Susceptibility
... were recruited between May 2005 and September 2009 from 40 pediatric clinics in Sweden [6]. At follow up, 95% of the patients were classified as T1D while the remaining 5% were classified with type 2, MODY, secondary, ‘‘other’’ or ‘‘unknown’’ type of diabetes. All patients of non-European descent we ...
... were recruited between May 2005 and September 2009 from 40 pediatric clinics in Sweden [6]. At follow up, 95% of the patients were classified as T1D while the remaining 5% were classified with type 2, MODY, secondary, ‘‘other’’ or ‘‘unknown’’ type of diabetes. All patients of non-European descent we ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse