
1. Science and God - How Do They Relate - hss-1.us
... The nuclear envelope disassembles and microtubules invade the nuclear space. This is called open mitosis, and it occurs in most multicellular organisms. Fungi and some protists, such as algae or trichomonads, undergo a variation called closed mitosis where the spindle forms inside the nucleus or its ...
... The nuclear envelope disassembles and microtubules invade the nuclear space. This is called open mitosis, and it occurs in most multicellular organisms. Fungi and some protists, such as algae or trichomonads, undergo a variation called closed mitosis where the spindle forms inside the nucleus or its ...
Mutations in an AP2 Transcription Factor
... expressed genes in leaves and stalks between dil1 mutant and wild type plants were identified in a microarray experiment (data not shown). Although many of the differentially expressed genes are involved in hormone metabolism or are hormone-responsive, it is virtually impossible to identify the gene ...
... expressed genes in leaves and stalks between dil1 mutant and wild type plants were identified in a microarray experiment (data not shown). Although many of the differentially expressed genes are involved in hormone metabolism or are hormone-responsive, it is virtually impossible to identify the gene ...
Phylogenetic analysis of the insect order Odonata using 28S and
... to multiple alignment with the CLUSTAL X program (Thompson et al. 1997) using the profile alignment mode, enforcing a secondary structure model of wasp 28S rRNA or insect 16S rRNA (Schmitz & Moritz 1998; Buckley et al. 2000) as the profiles. In the alignment procedure, gaps in stem structures were p ...
... to multiple alignment with the CLUSTAL X program (Thompson et al. 1997) using the profile alignment mode, enforcing a secondary structure model of wasp 28S rRNA or insect 16S rRNA (Schmitz & Moritz 1998; Buckley et al. 2000) as the profiles. In the alignment procedure, gaps in stem structures were p ...
Genetic Characterization and Inheritance of Belly Spot
... In the cell wall of the melanocyte several proteins are attached, among them Kit, a transmembrane tyrosine kinase receptor. Kit has a ligand called Kitligand, steel or stem cell growth factor (SCF) (Alberts B. et al, 2002). From previous studies we know that Kit is involved in fetal development. The ...
... In the cell wall of the melanocyte several proteins are attached, among them Kit, a transmembrane tyrosine kinase receptor. Kit has a ligand called Kitligand, steel or stem cell growth factor (SCF) (Alberts B. et al, 2002). From previous studies we know that Kit is involved in fetal development. The ...
The Neurospora crassa colonial temperature
... On the other hand, even though reduced calcineurin activity has already been shown to affect hyphal polarity in N. crassa and A. nidulans, no significant effect on hyphal growth of C. albicans was observed (Bader et al. 2003), despite the protein having a clear effect on pathogenicity. Calcineurin w ...
... On the other hand, even though reduced calcineurin activity has already been shown to affect hyphal polarity in N. crassa and A. nidulans, no significant effect on hyphal growth of C. albicans was observed (Bader et al. 2003), despite the protein having a clear effect on pathogenicity. Calcineurin w ...
The use of amplified fragment length polymorphism (AFLP) in the
... using AFLPs is the possible occurrence of null alleles, where the Y chromosome is present but the marker does not amplify. Large numbers of known-sex individuals of the heterogametic sex are needed to achieve acceptable small confidence limits on the occurrence of null alleles (e.g. 100 individuals ...
... using AFLPs is the possible occurrence of null alleles, where the Y chromosome is present but the marker does not amplify. Large numbers of known-sex individuals of the heterogametic sex are needed to achieve acceptable small confidence limits on the occurrence of null alleles (e.g. 100 individuals ...
PDF + SI - Development - The Company of Biologists
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. ...
... This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. ...
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.
... and further localized to a 65-kb region by restriction fragment length polymorphism (RFLP) mapping. A clone containing the ix gene was identified by its ability to rescue ix mutant phenotypes when introduced into flies by P-element-mediated germline transformation. The ix protein has sequence simila ...
... and further localized to a 65-kb region by restriction fragment length polymorphism (RFLP) mapping. A clone containing the ix gene was identified by its ability to rescue ix mutant phenotypes when introduced into flies by P-element-mediated germline transformation. The ix protein has sequence simila ...
The Effects of varying pH on Plasmid Transfer
... integration is a very simple way to mobilize other genetic material. The F plasmid of Escherichia coli is an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are cal ...
... integration is a very simple way to mobilize other genetic material. The F plasmid of Escherichia coli is an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are cal ...
When epigenetics meets alternative splicing: the roles of DNA
... might take part in the complex and highly regulated process of exon recognition. If cis-acting regulatory factors, such as splice-site sequences and splicing factors binding domains, did not provide enough complexity, splicing researchers are now realizing that the chromatin structure itself might a ...
... might take part in the complex and highly regulated process of exon recognition. If cis-acting regulatory factors, such as splice-site sequences and splicing factors binding domains, did not provide enough complexity, splicing researchers are now realizing that the chromatin structure itself might a ...
Molecular markers located on the DGAT1, CAST, and - Funpec-RP
... between SNPs and the previously described traits. Sire was fitted in the model as a random effect. The pdiff function of LSMEANS was utilized to evaluate significant differences in the performance of genotypes for SNPs that were identified as significant. All statistical analyses were conducted usin ...
... between SNPs and the previously described traits. Sire was fitted in the model as a random effect. The pdiff function of LSMEANS was utilized to evaluate significant differences in the performance of genotypes for SNPs that were identified as significant. All statistical analyses were conducted usin ...
Nutrigenomics in the Patient Care Process: Figuring Out the Puzzle
... • Some SNPs change the gene’s “instruction manual” - encoding a protein with altered shape, activity, stability and/or abundance • Only certain SNPs are associated with difference in molecular function significant enough to effect clinical measurements or disease risk • Genes that encode different e ...
... • Some SNPs change the gene’s “instruction manual” - encoding a protein with altered shape, activity, stability and/or abundance • Only certain SNPs are associated with difference in molecular function significant enough to effect clinical measurements or disease risk • Genes that encode different e ...
Genome Biology - Institute for Applied Ecology
... males [11]. Thus, the assumptions leading to Model I are questionable. In Model II, the amount of compensation was assumed to increase as a function of gene expression level in both sexes (since the level of expression of individual genes in the two sexes is highly correlated across a wide range of ...
... males [11]. Thus, the assumptions leading to Model I are questionable. In Model II, the amount of compensation was assumed to increase as a function of gene expression level in both sexes (since the level of expression of individual genes in the two sexes is highly correlated across a wide range of ...
doc - Lonely Joe Parker
... inferred from gene sequences can be erroneous (and may, as in the case of Prestin, resolve with strong support convergent taxa as sister taxons) in contrast to accepted species phylogenies. This observation suggests one test for convergence. If we use fixed topologies (instead of inferring phylogeni ...
... inferred from gene sequences can be erroneous (and may, as in the case of Prestin, resolve with strong support convergent taxa as sister taxons) in contrast to accepted species phylogenies. This observation suggests one test for convergence. If we use fixed topologies (instead of inferring phylogeni ...
Slides
... About 868,000 in human genome 6,500 base pairs long including LTRs Encode reverse transcriptase and integrase Copy-paste mechanism to insert elsewhere ...
... About 868,000 in human genome 6,500 base pairs long including LTRs Encode reverse transcriptase and integrase Copy-paste mechanism to insert elsewhere ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
... macrospora genomic DNA and cosmid DNAs revealed that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region ...
Coffee, B, Zhang, F, Warren, ST and Reines, D: Acetylated histones are associated with the FMR1 gene in normal but not fragile X syndrome cells. Nature Genetics 22:98-101 (1999).
... phenotype of fragile X syndrome. In normal cells, the FMR1 repeat is of normal length and methylation status, whereas those cells derived from patients exhibited repeat lengths in excess of 300 triplets and were hypermethylated. Cells were cultured in RPMI1640 media supplemented with 10% fetal calf ...
... phenotype of fragile X syndrome. In normal cells, the FMR1 repeat is of normal length and methylation status, whereas those cells derived from patients exhibited repeat lengths in excess of 300 triplets and were hypermethylated. Cells were cultured in RPMI1640 media supplemented with 10% fetal calf ...
Practical exon and gene quantification in R
... > elementMetadata(grng)[['A']] <- unlist(counts) In order to add more datasets to the grng object, the steps generating the bam and counts variables can simply be repeated and the resulting data added to the grng object. ...
... > elementMetadata(grng)[['A']] <- unlist(counts) In order to add more datasets to the grng object, the steps generating the bam and counts variables can simply be repeated and the resulting data added to the grng object. ...
Clinical Genetic Basis of Tooth Agenesis (PDF Available)
... expression properties. During mid gestation, Msx1 and Msx2 expressions occur at almost all sites of epithelialmesenchymal tissue interactions . At E11.5, Msx1 is coexpressed with Msx2 in the dental mesenchyme . However, the Msx1 is expressed quite broadly (in high levels) in the mesenchyme and the M ...
... expression properties. During mid gestation, Msx1 and Msx2 expressions occur at almost all sites of epithelialmesenchymal tissue interactions . At E11.5, Msx1 is coexpressed with Msx2 in the dental mesenchyme . However, the Msx1 is expressed quite broadly (in high levels) in the mesenchyme and the M ...
DHFR catalyzes the transfer of a hydride from NADPH to
... Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is f ...
... Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is f ...
Glucose-6-Phosphate Dehydrogenase (GGPD) Mutations
... most common human disease producing red blood cell enzyme deficiency.’ Nearly 400 biochemical variants have been described, but less than 70 mutations have been identified attheDNA level. We2-5and others”’ have previously shown that at least nine different types of mutation are responsible for G6PD ...
... most common human disease producing red blood cell enzyme deficiency.’ Nearly 400 biochemical variants have been described, but less than 70 mutations have been identified attheDNA level. We2-5and others”’ have previously shown that at least nine different types of mutation are responsible for G6PD ...
Molecualr Biology and Evolution
... restriction map was made of pAR2 and used to generate lies within the proteobacterial clade with bootstrap sup- smaller subclones of the n$K region in pUCl19. port of 80%. Normand et al. ( 1992) provided an additional test of the horizontal transfer hypothesis by ex- Sequencing amining the phylogeny ...
... restriction map was made of pAR2 and used to generate lies within the proteobacterial clade with bootstrap sup- smaller subclones of the n$K region in pUCl19. port of 80%. Normand et al. ( 1992) provided an additional test of the horizontal transfer hypothesis by ex- Sequencing amining the phylogeny ...
Site-specific recombinase technology

Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse