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Noonan
Noonan

... RAD21 (cohesin): marks insulators, promoters and enhancers ...
Gene Expression—Transcription
Gene Expression—Transcription

... Introns are sections of pre-mRNA that are noncoding. That is, they don’t provide useful information for the production of the polypeptide being synthesized. There is evidence that suggests these introns allow certain sections of DNA to code for different polypeptides when different sections are remo ...
Transcription Coactivator Family Proteins
Transcription Coactivator Family Proteins

... general transcription factors. While direct contacts between activators and general factors have been demonstrated in vitro, an additional class of proteins, termed coactivators, appear to be required for transcriptional activation of some genes. For example, transcription of class II genes depends ...
Chapter 11 - Evangel University
Chapter 11 - Evangel University

... after transcription to give the functional form • the initial size of the RNA transcript is greater than the final size because of the leader sequences at the 5’ end and the trailer sequences at the 3’ end • the types of processing in prokaryotes can differ greatly from that in eukaryotes, especiall ...
Document
Document

... – moncistronic: one mRNA codes for only one polypeptide • 3 RNA polymerases in euk., 1 in prok. Binding of Basal Transcription Factors required for euk. RNA Pol II binding. • Processing of mRNA in eukaryotes: – 5’ 7-methylguanosine (7mG) cap added – 3’ Poly-A tail added – Splicing out of introns ...
Lecture 10/11/06
Lecture 10/11/06

... ƒ There’s a repressor protein. ƒ Also, the mRNA binds with itself where there is complementarity downstream. There’s a Trp Leader that controls via attenuation. If there’s a lot of tryptophan, then the ribosomes work pretty close to the RNA polymerase, which causes the mRNA to bind to itself right n ...
Protein Synthesis
Protein Synthesis

... transfer RNA (tRNA) which transfers the amino acid to the ribosome where mRNA awaits The key components of the tRNA molecule that are important during translation are amino acids and anticodons ...
Ontology Alignment
Ontology Alignment

... – Synthesis of gene products (RNA and proteins) – Two steps: transcription and translation – Transcription: Gene  RNA (mediated by transcription factor proteins (TF) that regulate (up / down) the synthesis of RNA by a polymerase enzyme) – Translation: RNA  protein ...
RNA Transcription
RNA Transcription

... acid to protein, may be possible, but transfer from protein to protein, or from protein to nucleic acid, is impossible. Information means here the precise determination of sequence, either of bases in the nucleic acid or of amino acid residues in the protein.“ Francis Crick, 1958 ...
foga i - is there such a thing as a gene? = formatting the
foga i - is there such a thing as a gene? = formatting the

... molecular computations in cells * Layering of weak and “fuzzy” interactions provides overall precision to integrated cellular responses * Allostery( the fact that binding of one ligand affects binding a distinct ligand) confers communication and processing capabilities on individual molecules * Repe ...
the ubiquitin system and a putative stimulatory role
the ubiquitin system and a putative stimulatory role

... Among eukaryotes, ubiquitin is highly conserved, meaning that the amino acid sequence does not differ much when very different organisms are compared. Ub is a heat-stable protein that folds up into a compact globular structure. It is found throughout the cell and can exist either in free form or as ...
Transcription Regulation
Transcription Regulation

... • The promotors possibly interact physically or have related functions at multiple genes. ...
Gene Control
Gene Control

... Distal control elements = enhancers  a. may be up or down stream  b. each gene can have many enhancers ...
CXXC5 plays a role as a transcription activator for myelin genes
CXXC5 plays a role as a transcription activator for myelin genes

... Myelination in corpus callosum plays important role for normal brain functions by transferring neurological information between various brain regions. However, the factors controlling expression of myelin genes in myelination are poorly understood. Here, CXXC5, a recently identified protein with CXX ...
Gene expression and regulation
Gene expression and regulation

... cytoplasm at which this occurs is called a ribosome. Ribosomes are a mixture of ribosomal proteins and ribosomal RNA (rRNA), and consist of a large subunit and a small subunit. ...
Negative regulation of eukaryotic transcription
Negative regulation of eukaryotic transcription

... sections, and in order to operate in this way the repressor binding sites must have a specific positional relationship with the activator binding sites. The fourth type of transcriptional repressor acts via DNA elements that are position independent, and by analogy with enhancers are termed silencer ...
Chapter 11 Gene Expression
Chapter 11 Gene Expression

... 2) Cells use information in genes to build hundreds of different proteins, each with a unique function, but not all proteins are required by the cell at one time By regulating gene expression, cells are able to control when each protein is made a. Some proteins play structural roles, others are enzy ...
TITLE OF MODULE: From Gene to Function MODULE NUMBER
TITLE OF MODULE: From Gene to Function MODULE NUMBER

... with specific examples. (ALJ) Lecture 25. Nuclear export, cytoplasmic localisation and RNA decay. How is mRNA exported from the nucleus. Mechanism and significance of specific mRNA localisation in the cytoplasm. Pathways of mRNA decay with an emphasis on quality control mechanisms. (ALJ) Lecture 26. ...
Transcription
Transcription

... The Central Dogma ...
Regulation of ISWI -family of chromatin remodelling complexes
Regulation of ISWI -family of chromatin remodelling complexes

... mechanisms that eukaryotic cells have evolved to induce structural changes to chromatin. All ISWIcontaining complexes use the energy derived from ATP hydrolysis in order to rearrange nucleosomes on chromatin to carry specific nuclear reactions. The combination of associated proteins with the ATPase ...
Transcription - Effingham County Schools
Transcription - Effingham County Schools

... make a copy of it for your own use, put the reference material back on the shelf so that others can use it too. Can you imagine if DNA was physically lost? That’s why chromosomes never leave the nucleus. ...
Transcriptional regulatory roles of G
Transcriptional regulatory roles of G

... genes using both computational and a wide range of experimental approaches. Our bioinformatics study has revealed that potential G4-forming sequences are particularly enriched in the transcription regulatory regions (TRRs) of cardiac function-related genes. Several genes (adrenergic receptor beta-1 ...
Chapter 18: Control of Gene Expression in Bacteria
Chapter 18: Control of Gene Expression in Bacteria

... 2.) What is the function of the proteins Galactoside permease and β-Galactosidase? 3.) What is an operon? 4.) What are the genes encoded by the lac operon promoter? Is lacI transcribed from the same lac operon promoter? Which genes express β-Galactosidase and Galactoside permease? 5.) What is an Ope ...
Protein Synthesis
Protein Synthesis

... • The newly made RNA strand detaches from the DNA after the gene is transcribed. • The small RNA strand exits the nucleus through pores in the nuclear membrane ...
Structure/function relationship in DNA
Structure/function relationship in DNA

... Classic ZnF is ~30 amino-acid domain ...
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Histone acetylation and deacetylation



Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.
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