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The rapidly emerging ESBL-producing Escherichia coli O25
The rapidly emerging ESBL-producing Escherichia coli O25

... titers found in the population earlier (Gibbs et al., 2004) could have contributed to a permissive environment allowing the rapid spread of the K-12 core-containing strains, such as the members of ST131 clone, in the gut and in extraintestinal niches. As most of the epidemiological studies revealing ...
Transcript  - Howard Hughes Medical Institute
Transcript - Howard Hughes Medical Institute

... through the data. And then in 1997 another family was identified where there were three affected girls with Rett syndrome. This family was in Brazil. Again, the Family Association brought them here and helped researchers study them. So when you look at these four families now, perhaps, you can refin ...
NEAT / HyperNEAT
NEAT / HyperNEAT

... Keep a global counter; every time a neuron or synapse is added, assign the value of the counter, and increment it. Genes/synapses can be disabled, but remain in the genome. ...
Characterization of the amino acid response element within the
Characterization of the amino acid response element within the

... Taq polymerase and amplification of 35 cycles of 95 ◦C for 15 s, and 58 ◦C for 60 s. After PCR, melting curves were acquired by stepwise increase of the temperature from 55 to 95 ◦C to ensure that a single product was amplified in the reaction. From the same RNA samples used to assess transcriptiona ...
entire lesson plan PDF
entire lesson plan PDF

... is taken from one organism and inserted into the cells of another organism, often of a different species. Genetic engineering can also be a rearrangement of the location of genes. The new “altered” organism then makes new substances or performs new functions based on its new DNA. For example, the pr ...
VI. The relationship between genotype and phenotype is rarely simple
VI. The relationship between genotype and phenotype is rarely simple

... 9. Define random event, and explain why it is significant that allele segregation during meiosis and fusion of gametes at fertilization are random events. 10. Use the rule of multiplication to calculate the probability that a particular F2 individual will be homozygous recessive or dominant. 11. Giv ...
Homozygous hemoglobin Knossos (alpha 2 beta 227(B9) Ala---
Homozygous hemoglobin Knossos (alpha 2 beta 227(B9) Ala---

Biochemistry and biosynthesis of insect pigments
Biochemistry and biosynthesis of insect pigments

... The earliest reference to carmine produced by cochineal insects, such as Dactylopius coccus (Hemiptera: Dactylopiidae), D. confuses (Hemiptera: Dactylopiidae), Porphyrophora polonica (Hemiptera: Margarodidae) and P. hamelii (Hemiptera: Margarodidae), dates back to 714 B.C. Marcin (1595) of Urzędów r ...
187-192. Control of transcription by Pontin and Reptin
187-192. Control of transcription by Pontin and Reptin

... Pontin and Reptin are two closely related members of the AAA+ family of DNA helicases. They play roles in diverse cellular processes, including the response to DNA double-strand breaks and the control of gene expression. The two proteins share residence in different multi-protein complexes, such as ...
Genetic Basis of the Variegated Tail Pattern in the Guppy, Poecilia
Genetic Basis of the Variegated Tail Pattern in the Guppy, Poecilia

... Inheritance of the variegated tail pattern was elucidated by singlepair reciprocal crosses between the GV strain and WT stock, using six-week old mature virgin fish. Each pair was kept in a 3.5-liter breeding tank. Broods were produced 4-6 weeks after mating. Single-pair full-sib F1 males and F1 fem ...
Gene expression analysis
Gene expression analysis

... Ulf Leser and Karin Zimmermann: Bioinformatics, Wintersemester 2010/2011 ...
Applet for calculating heritability for threshold traits
Applet for calculating heritability for threshold traits

... Chapter 12. Estimating- and biotechnology and disease resistance........................................................ 88 12.1 Technology for estimation of breeding value ............................................................................. 88 12.2 The significance of artificial inseminati ...
The Complex Inheritance of Maize Domestication Traits and Gene
The Complex Inheritance of Maize Domestication Traits and Gene

... was given to shepherd them to completion. Every day and conversation I have had with John as my advisor has made me into a better scientist and I am extremely thankful for the opportunity I was given six years ago when I joined the Doebley lab. I have been fortunate enough to also work in an outstan ...
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a

... response was targeted to these elements as a means of containing potential genomic instability. We used ChIP coupled with next generation sequencing (ChIP-Seq) to determine the genomic localization of the H3K9me3 response. Although there was a general increase in this response across the genome, our ...
Bioinformatics Dr. Víctor Treviño  Pabellón Tec
Bioinformatics Dr. Víctor Treviño Pabellón Tec

... Procedure for comparing two (pair-wise alignment) or more (multiple sequence alignment) sequences by searching for similar patterns that are in the same order in the sequences ...
module 5: translation - GEP Community Server
module 5: translation - GEP Community Server

... consecutive bases), there are six possible reading frames. One can determine a reading frame by dividing the sequence of nucleotides in DNA or RNA into a set of consecutive, non-overlapping triplets. There are three possible reading frames (read 5’3’) in the forward direction on the top strand of D ...
Genetics of Skin Colour
Genetics of Skin Colour

... the genetic material in skin cells and induce skin cancer. Because most sun induced skin cancers develop well past the reproductive age in people older than 50 years, skin cancer and protection from sunburn are unlikely to have been significant evolutionary factors for the natural selection of ances ...
Figure 5 - GEP Community Server
Figure 5 - GEP Community Server

... consecutive bases), there are six possible reading frames. One can determine a reading frame by dividing the sequence of nucleotides in DNA or RNA into a set of consecutive, non-overlapping triplets. There are three possible reading frames (read 5’3’) in the forward direction on the top strand of D ...
A versatile toolbox for PCR-based tagging of yeast genes: new
A versatile toolbox for PCR-based tagging of yeast genes: new

... new selection markers have been introduced. The tags include the so far brightest and most yeast-optimized version of the red fluorescent protein, called RedStar2, as well as all other commonly used fluorescent proteins and tags used for the detection and purification of proteins and protein complex ...
Gadd45g Is Essential for Primary Sex Determination, Male
Gadd45g Is Essential for Primary Sex Determination, Male

... [6]. They are implicated in the regulation of apoptosis, survival, senescence, cell cycle control, DNA repair and the response to physiological or environmental stress in mammalian cells. All family members appear to have overlapping but non-identical functions and binding partners, and are induced ...
Differential Functional Variability of Serotonin Transporter and
Differential Functional Variability of Serotonin Transporter and

Missouri Western State University
Missouri Western State University

... first and second, second and third, all three. • The following slide shows Northern Hemisphere of the 2-spatula burnt pancake graph on a globe. ...
- Zurich Open Repository and Archive
- Zurich Open Repository and Archive

... metal-chelating substance, respectively. Loss of the green color occurs during the subsequent opening of the porphyrin macrocycle of pheophorbide (pheide) a by the coupled action of pheide a oxygenase (PAO) and red chl catabolite reductase (RCCR). This yields a primary fluorescent catabolite (pFCC), ...
TBK1 Gene Duplication and Normal
TBK1 Gene Duplication and Normal

... sum of these data strongly suggest that duplication of TBK1 causes 0.4% to 1.3% of NTG cases.18,19 However, animal and/or functional studies will be required to definitively prove that chromosome 12q14 duplications cause NTG by altering the function of TBK1 rather than through effects on other neigh ...
The Mitochondrial Genome of Chara vulgaris
The Mitochondrial Genome of Chara vulgaris

... streptophyte mtDNAs suggests that introns have been gained independently in several lineages. Of the 11 introns in Chaetosphaeridium mtDNA, only the fourth intron in cox1 exhibits positional and structural conservation with one of the seven introns in Mesostigma mtDNA, and only the second intron in ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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