Whole genome analyses using PopGenome and VCF files
... the parameter format have to be set to format="VCF". In contrast to the readVCF function the data does not need to be compressed or tabixed and additionaly supports polyploid (e.g tetraploid) genotypes. However, each VCF file is completely loaded into the RAM and interpreted via efficient C Code wh ...
... the parameter format have to be set to format="VCF". In contrast to the readVCF function the data does not need to be compressed or tabixed and additionaly supports polyploid (e.g tetraploid) genotypes. However, each VCF file is completely loaded into the RAM and interpreted via efficient C Code wh ...
The Advantage and Application of Genetically Humanized Mouse
... or tissue-specific transgene expression and inserted randomly into the genome. Alternatively, cDNA containing the human transgene and small promoter fragment can be targeted to a specific location within the genome. Since cDNA constructs lack the non-coding and regulatory elements found in genomic D ...
... or tissue-specific transgene expression and inserted randomly into the genome. Alternatively, cDNA containing the human transgene and small promoter fragment can be targeted to a specific location within the genome. Since cDNA constructs lack the non-coding and regulatory elements found in genomic D ...
Accelerated Evolution of Sex Chromosomes in
... Sex chromosomes play a role in many important biological processes, including sex determination, genomic conflicts, imprinting, and speciation. In particular, they exhibit several unusual properties such as inheritance pattern, hemizygosity, and reduced recombination, which influence their response ...
... Sex chromosomes play a role in many important biological processes, including sex determination, genomic conflicts, imprinting, and speciation. In particular, they exhibit several unusual properties such as inheritance pattern, hemizygosity, and reduced recombination, which influence their response ...
Analysis of skin color change and related gene
... ISA B-line layers were obtained from Guangda poultry breeding company in Zhejiang Province, and animals had been bred for 15 generations. Dongxiang black chickens collected from Dongxiang County in Jiangxi Province had been bred for 12 generations at Guangda poultry breeding company. Ten Dongxiang c ...
... ISA B-line layers were obtained from Guangda poultry breeding company in Zhejiang Province, and animals had been bred for 15 generations. Dongxiang black chickens collected from Dongxiang County in Jiangxi Province had been bred for 12 generations at Guangda poultry breeding company. Ten Dongxiang c ...
Snímek 1
... reduced function but not loss of function. The loss of amino acids that are encoded by exon 7 results in the production of SMN protein with severely decreased oligomerization efficiency and stability. The SMN monomers are rapidly degraded. Thus, loss of SMN1 results in reduction of SMN levels in mos ...
... reduced function but not loss of function. The loss of amino acids that are encoded by exon 7 results in the production of SMN protein with severely decreased oligomerization efficiency and stability. The SMN monomers are rapidly degraded. Thus, loss of SMN1 results in reduction of SMN levels in mos ...
Unsaturated Fatty Acids, Desaturases, and Human Health
... consequent LCPUFA deficiency, which led to the abnormal conditions of reproduction, skin and intestine without affecting viability.22,23 The knockout mice also displayed tissue-dependent changes in LCPUFA contents. However, ARA supplementation in a diet reversed the dermatitis and intestinal ulcers ...
... consequent LCPUFA deficiency, which led to the abnormal conditions of reproduction, skin and intestine without affecting viability.22,23 The knockout mice also displayed tissue-dependent changes in LCPUFA contents. However, ARA supplementation in a diet reversed the dermatitis and intestinal ulcers ...
human tRNA(m22G26)dimethyltransferase: functional expression
... are active at only one single site, producing one specific product (12–15). Others have multisite specificity, i.e. one enzyme mediates the formation of one specific modification, but does so at more than one site (16–19). The yeast enzyme tRNA(m22G)dimethyltransferase, encoded by a single nuclear T ...
... are active at only one single site, producing one specific product (12–15). Others have multisite specificity, i.e. one enzyme mediates the formation of one specific modification, but does so at more than one site (16–19). The yeast enzyme tRNA(m22G)dimethyltransferase, encoded by a single nuclear T ...
THE RESPONSE TO ARTIFICIAL SELECTION DUE TO
... In addition to the total response estimated in each instance from 1000 replicate computer runs, the following variables have been simultaneously recorded: ;).qO, the initial rate of change in gene frequency per generation, estimated as the mean over the first five generations of selection; (2) L50 a ...
... In addition to the total response estimated in each instance from 1000 replicate computer runs, the following variables have been simultaneously recorded: ;).qO, the initial rate of change in gene frequency per generation, estimated as the mean over the first five generations of selection; (2) L50 a ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... + Then we form a set of “potential genes” and “potential non-genes” by classifying each ORF sequence into the first set if it exhibits significant evidence of 3-periodicity with respect to some DNA word(s) (i.e., if the value of R(3) is below some appropriate threshold), and by classifying it into t ...
... + Then we form a set of “potential genes” and “potential non-genes” by classifying each ORF sequence into the first set if it exhibits significant evidence of 3-periodicity with respect to some DNA word(s) (i.e., if the value of R(3) is below some appropriate threshold), and by classifying it into t ...
Artificial selection on introduced Asian haplotypes shaped the
... in order to improve traits of commercial interest. Current genomics techniques enabled genome-wide identification of these Asian introgressed haplotypes in modern European pig breeds. We propose that the Asian variants are still present because they affect phenotypes that were important for ancient ...
... in order to improve traits of commercial interest. Current genomics techniques enabled genome-wide identification of these Asian introgressed haplotypes in modern European pig breeds. We propose that the Asian variants are still present because they affect phenotypes that were important for ancient ...
Regulation of 6sg expression site transcription and switching in
... bloodstream forms, the replacement rRNA promoter was down-regulated in a ‘silent’ ES but it was active in procyclic forms [1,2]. In addition to manipulations of endogenous promoters, we have recently shown that, when an ES promoter is replaced by a T7 promoter, the T7 promoter is unregulated but tra ...
... bloodstream forms, the replacement rRNA promoter was down-regulated in a ‘silent’ ES but it was active in procyclic forms [1,2]. In addition to manipulations of endogenous promoters, we have recently shown that, when an ES promoter is replaced by a T7 promoter, the T7 promoter is unregulated but tra ...
CHAPTER FOUR U n
... for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess against a second population, which is the “tester”, to remove common (hybridizing) sequence ...
... for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess against a second population, which is the “tester”, to remove common (hybridizing) sequence ...
Advances in genetics show the need for extending screening
... for the (ADH) studies in which a LOD score .3.3 is required (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial ...
... for the (ADH) studies in which a LOD score .3.3 is required (threshold for complex traits), since this means that a large number of individuals is needed for the analysis.15,16 Another means of identifying novel ADH genes is through genome-wide association studies. This approach received substantial ...
ISOLATION AND FUNCTIONAL GENETIC EUCALYPTUS Magister Scientiae
... properties such as lignin content and composition. Paper quality can also be improved by changing wood properties such as microfibril angles. However, our understanding of the wood formation process is far from complete. This is an obstacle for us to genetically engineer wood properties of Eucalyptu ...
... properties such as lignin content and composition. Paper quality can also be improved by changing wood properties such as microfibril angles. However, our understanding of the wood formation process is far from complete. This is an obstacle for us to genetically engineer wood properties of Eucalyptu ...
Codon usage bias from tRNA`s point of view
... The horizontal dashed line indicates the expected value if there was no change in G+C anticodon composition with genome G+C content. The linear regression of the observed data is shown as a solid black line (it has an R2 of 0.50 and a slope of 0.26, significantly different from 1 and 0, P < 0.001). ...
... The horizontal dashed line indicates the expected value if there was no change in G+C anticodon composition with genome G+C content. The linear regression of the observed data is shown as a solid black line (it has an R2 of 0.50 and a slope of 0.26, significantly different from 1 and 0, P < 0.001). ...
Lab 7. Mendelian Genetics
... In 1866 an Austrian monk, Gregor Mendel, presented the results of painstaking experiments on the inheritance patterns of garden peas. Those results were heard, but probably not understood, by Mendel’s audience. Now, more than a century later, Mendel’s work seems elementary to modern–day geneticists, ...
... In 1866 an Austrian monk, Gregor Mendel, presented the results of painstaking experiments on the inheritance patterns of garden peas. Those results were heard, but probably not understood, by Mendel’s audience. Now, more than a century later, Mendel’s work seems elementary to modern–day geneticists, ...
The SELF-PRUNING gene of tomato regulates
... because the ‘determinate’ growth habit facilitates mechanical harvest. A principal assumption of our work is that Arabidopsis, Antirrhinum and tomato employ similar genes to regulate their ...
... because the ‘determinate’ growth habit facilitates mechanical harvest. A principal assumption of our work is that Arabidopsis, Antirrhinum and tomato employ similar genes to regulate their ...
04/01/15 lecture2_04c
... in favor of the alternative. If it is greater than 0.05 then accept the null hypothesis. (2) If the null hypothesis is not a special case of the alternative use the AIC criterion to compare. For example, a dominant Mendelian model under HWE is not a special case of a recessive Mendelian model where ...
... in favor of the alternative. If it is greater than 0.05 then accept the null hypothesis. (2) If the null hypothesis is not a special case of the alternative use the AIC criterion to compare. For example, a dominant Mendelian model under HWE is not a special case of a recessive Mendelian model where ...
Formation of synovial joints and articular cartilage
... neutral environment, but not in non-neutral environment. It occurs in the embryo once skeletogenic mesenchymal cells have condensed into precartilaginous masses. Most cells in these masses commit to the growth plate chondrocyte fate, while specific subpopulations commit to the articular fate and go ...
... neutral environment, but not in non-neutral environment. It occurs in the embryo once skeletogenic mesenchymal cells have condensed into precartilaginous masses. Most cells in these masses commit to the growth plate chondrocyte fate, while specific subpopulations commit to the articular fate and go ...
Red/ET Recombination
... Recombineering therefore permits more DNA engineering freedom than any other technology and has become an essential component of the molecular biological tool kit. Recombineering can easily be deployed alongside conventional DNA methodologies to give you time to do your research. From its headquarte ...
... Recombineering therefore permits more DNA engineering freedom than any other technology and has become an essential component of the molecular biological tool kit. Recombineering can easily be deployed alongside conventional DNA methodologies to give you time to do your research. From its headquarte ...
Document
... Integrates ontologies “as is”, including legacy ontologies Facilitates the integration of the corresponding datasets ...
... Integrates ontologies “as is”, including legacy ontologies Facilitates the integration of the corresponding datasets ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... gene into the yeast genome; as the foreign DNA provides no homology for recombination with the yeast chromosome, integration could be directed to the locus of the selectable marker. Excision of the inserted DNA from the chromosome also takes place by homologous recombination. Therefore, recombinatio ...
... gene into the yeast genome; as the foreign DNA provides no homology for recombination with the yeast chromosome, integration could be directed to the locus of the selectable marker. Excision of the inserted DNA from the chromosome also takes place by homologous recombination. Therefore, recombinatio ...
PDF - Journal of Genomics
... Native to southeastern and eastern Asia, the species was first detected in Wisconsin in the summer of 2000 and has quickly spread throughout much of the North Central United States and Eastern Canada (17, 18). Host-plant resistance (referring to the plant's ability to resist damaging insect invasion ...
... Native to southeastern and eastern Asia, the species was first detected in Wisconsin in the summer of 2000 and has quickly spread throughout much of the North Central United States and Eastern Canada (17, 18). Host-plant resistance (referring to the plant's ability to resist damaging insect invasion ...
The Normal Uniform Differential Gene Expression
... compared to the plot of the normalized log ratios, lRnorm, produced by the normalization method versus the log intensities, lI, (along with the loess fitted mean lines of both). > #lRnorm is the mean and variance normalized log ratios for the genes ...
... compared to the plot of the normalized log ratios, lRnorm, produced by the normalization method versus the log intensities, lI, (along with the loess fitted mean lines of both). > #lRnorm is the mean and variance normalized log ratios for the genes ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.