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... been revised from 5154 to 2222… – FANTOM/RIKEN Consortium Science, March 2006 Brendan Frey ...
Using Transcriptional Control To Increase Titers of Secreted
Using Transcriptional Control To Increase Titers of Secreted

... acterial heterologous protein production is a bedrock of modern molecular biology. A gene that codes for a protein of interest can be inserted into a bacterial cell, often Escherichia coli, and the cell is able to produce the desired protein. Bacteria are the preferred organisms for many heterologou ...
Chapter 14 – Mendel and the Gene Idea
Chapter 14 – Mendel and the Gene Idea

Foundations of Biology - Geoscience Research Institute
Foundations of Biology - Geoscience Research Institute

... Some gene products are needed by all cells all the time. These constitutive genes are expressed by all cells. Other genes are only needed by certain cells or at specific times, expression of these inducible genes is tightly controlled in most cells. For example, pancreatic b cells make insulin by ex ...
Number 48, 2001 35
Number 48, 2001 35

... ascospores generated in the barren crosses may be the ones, in which RIP either had not occurred or was very inefficient. In agreement with this possibility, a low frequency of mutants for loci covered by the duplication was observed, amongst the survivors (Perkins et al. 1997 Genetics 147:125-136). ...
CONSERVATION AND DIVERGENCE IN MOLECULAR
CONSERVATION AND DIVERGENCE IN MOLECULAR

... axis depends upon the activity of the dorsal transcription factor. During oogenesis, a molecular cue localized around the oocyte nucleus determines follicle cells lying on one side of the oocyte as dorsal. This leads to the development of follicle cells on the other side of the oocyte as ventral, vi ...
Stage-specific expressions of four different ribonuclease H genes in
Stage-specific expressions of four different ribonuclease H genes in

... The human pathogen of the genus Leishmania cause worldwide morbidity and infection of visceral organs by some species may be lethal. Lack of rational chemotherapy against these pathogens dictates the study of differential biochemistry and molecular biology of the parasite as compared to its human ho ...
The nucleotide sequence of Saccharomyces cerevisiae
The nucleotide sequence of Saccharomyces cerevisiae

... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
RNAi phenotypes are influenced by the genetic background of the
RNAi phenotypes are influenced by the genetic background of the

... different phenotypes depending on the injected strain. We rule out off target effects and show that sequence divergence does not account for this difference. By quantitatively comparing phenotypes elicited by RNAi knockdown of four different genes we show that there is no general difference in RNAi ...
Genetics
Genetics

... (1) If the F1 self-pollinated, what is the expected ratio between purple to white color plants in the F2 population? Purple : white = (1) If F1 is backcrossed to Parent 1, what is the expected ratio between purple to white color plants in BC1F1(P1) population? Purple : white = (1) If F1 is backcross ...
The Drosophila pipsqueak gene encodes a nuclear BTB
The Drosophila pipsqueak gene encodes a nuclear BTB

... suggested that the psq embryonic defect in posterior patterning might be due to insufficient expression of the maternal posterior group gene vasa in psq mutants (Siegel et al., 1993). In addition, we have shown that certain psq alleles also exhibit dorsal/ventral defects in eggshell structure (Horow ...
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in
Mutation Analysis of the Ornithine Transcarbamylase (OTC) Gene in

... Case 4, a heterozygote for the novel splice site mutation (c.386+1G>C), was also female. Although the c.386+1G>C mutation has not been previously reported, c.386+1G>T and c.386+1G>A have been found in the patients with OTC deficiency [48]. Furthermore, this nucleotide change disrupted a highly conse ...
Inheritance of Colour in Cattle
Inheritance of Colour in Cattle

... ! Where both are the same genes, the animal is Homozygous for that gene and will “breed true” for that gene, as it can only pass on one of that type to its progeny. (eg RbRb = Homozygous for Black) ! Where both are different genes, the animal is Heterozygous for that gene and will “not breed true” f ...
Are Incomplete Denitrification Pathways a Common Trait in Thermus
Are Incomplete Denitrification Pathways a Common Trait in Thermus

... 60 °C. The primary focus of this study was to investigate the denitrification pathways of Thermus (Bacteria) isolates from geothermal springs from Tengchong, China. This study tested the hypothesis that incomplete denitrification is a common characteristic of the genus Thermus, regardless of geograp ...
Archaeal phylogenomics provides evidence in support of a
Archaeal phylogenomics provides evidence in support of a

... [20]. Other large multi-gene approaches have rendered hypotheses that the eukaryotes descend from an ancient and uncharacterized archaeal lineage [12]. However, most phylogenetic reconstructions using informational gene sequence data recover a root for the eukaryotes in the Crenarchaea: known as the ...
Scientific background report AMFLORA potato
Scientific background report AMFLORA potato

... from the potato and as a result of that becomes resistant against kanamycin and neomycin? This chance is immensely small. Transfer of antibiotic resistant genes from transgenic plants to bacteria has neither been demonstrated in nature, nor in the laboratory. Only in the case that the bacterium alre ...
PDF
PDF

... [5], providing the opportunity to compare the mouse and human genomes. Comparing the human genome with the mouse genome can greatly help our understanding of both genomes. We used the BLASTN program [6] to compare the December 2001 golden path freeze of the human genome, which is also NCBI build 28, ...
- Ex Student Archive
- Ex Student Archive

... Apart from the logical association to sensory meat characteristics, the term meat quality may also refer to several other aspects. The wholesomeness in meat quality is an important aspect which may refer to the products´ nutritional value and to microbial and/or chemical food safety. High meat quali ...
Protein-protein interaction networks (I): data preprocessing
Protein-protein interaction networks (I): data preprocessing

... A data set of high coverage is not very useful if its accuracy is low, i.e. if it contains a large number of false positives.Similarly, a data set of high accuracy is not very useful if its coverage is low. Commonly, one estimates accuracy and coverage by comparing to a reference set of known intera ...
Transcription
Transcription

... Some gene products are needed by all cells all the time. These constitutive genes are expressed by all cells. Other genes are only needed by certain cells or at specific times, expression of these inducible genes is tightly controlled in most cells. For example, pancreatic b cells make insulin by ex ...
Package `BitSeq`
Package `BitSeq`

... reduction of the marginal posterior variance. While the possible scale reduction is high, it doubles the MCMC_samplesN and starts new iteration. This process is repeated until desired value of MCMC_scaleReduction is met, or MCMC_samplesNmax samples are generated. The sampling algorithm can be config ...
fulltext - DiVA portal
fulltext - DiVA portal

... formation in a calcium-independent fashion. ClyA is the only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2-7]. The clyA+ transcription is known to be subjected to transcriptional silencing by the H-NS nucleoid protein in ...
Making Headway: The Roles of Hox Genes and Neural
Making Headway: The Roles of Hox Genes and Neural

... of developmentally regulated transcription factors that are characterized by a conserved DNA binding motif called the homeobox. Hox genes are organized into a single chromosomal cluster in invertebrates, in contrast to higher vertebrates such as the mouse which have 39 Hox genes, organized into four ...
Genetics of Hemophilia
Genetics of Hemophilia

... does not produce the clotting factor he needs because the Y chromosome does not provide information for the production of factor VIII or IX. When a female inherits a gene that causes hemophilia on one of her X chromosomes, she has a second X chromosome, and therefore one normal gene that produces cl ...


... The Atlas of Genetics and Cytogenetics in Oncology and Haematology is a peer reviewed on-line journal in open access, devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases. It presents structured review articles (“cards”) on genes, leukaemias, solid tumours, canc ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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