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C2005/F2401 `07 -- Lecture 15 -- Last Edited
C2005/F2401 `07 -- Lecture 15 -- Last Edited

... a. There is a different repressor protein for each operon. Repressor binds to specific sequence of DNA found in its respective operator. b. Synthesis of repressor protein is constitutive -- gene is always on. (State of repressor protein varies, not the amount; see below.) Question: Does the gene for ...
BRCA1 and BRCA2 genes: What You Need to Know
BRCA1 and BRCA2 genes: What You Need to Know

CAFE: Computational Analysis of gene Family Evolution
CAFE: Computational Analysis of gene Family Evolution

... mcl understands. Then we give the .abc file to mcxload, which creates a network and a dictionary file (.mci and .tab, respectively). These two files are then used by mcl to perform the clustering, which is done with the last two commands. The -I (inflation) parameter determines how granular the clus ...
Lesson Plans
Lesson Plans

... recessive traits. Class discussion should uncover the fact that dominance refers only to which trait is found in an individual who has both types of alleles. A striking example of a human trait that is rare, although dominant, is polydactyly, or extra digits on the hand. When asked, most students wi ...
PPT - Bruce Blumberg
PPT - Bruce Blumberg

... – purify cellular proteins that interact with your protein • co-immunoprecipitation • affinity chromatography • biochemical fractionation – pure protein(s) are microsequenced • if not in database then make oligonucleotides and screen cDNA library from appropriate tissues – advantage • functional app ...
Onset of lactation in the bovine mammary gland:
Onset of lactation in the bovine mammary gland:

... Abstract The mammary gland undergoes dramatic functional and metabolic changes during the transition from late pregnancy to lactation. To better understand the molecular events underlying these changes, we analyzed expression profiles of approximately 23,000 gene transcripts in bovine mammary tissue ...
Introduction of an Active DNA Microarray Fabrication for Medical
Introduction of an Active DNA Microarray Fabrication for Medical

... The ink-jetting technology is a non-contact, data-driven deposition method which combining DNA systhesis chemistry with commercial ink-jet technology. Using this technology the spheres of fluid can be dispensed with diameters of 15 to 1 0 0 µm at rate of 0-25,000 per second from a single drop-on-dem ...
1 Comp. Funct. Genom. Copyright © (2002) John Wiley & Sons, Ltd.
1 Comp. Funct. Genom. Copyright © (2002) John Wiley & Sons, Ltd.

... genes and proteins based on shared biology. It will also aid the interpretation of large datasets created by functional genomics projects [6]. The majority of eukaryotic genome projects already use the GO annotation system, and GO annotations are being incorporated into SWISSPROT and GeneDB (see sec ...
RNA Processing
RNA Processing

... similar to that occurring four times in epidermal growth factor as well as in other proteins 3. Five exons encode a 400-residue sequence that 33% identical with a polypeptide segment shared with EGF ...
The varied roles of nuclear receptors during vertebrate embryonic
The varied roles of nuclear receptors during vertebrate embryonic

... Attribution License, which permits unrestricted non-commercial use distribution and reproduction in any medium, provided the original work is properly cited. Cite this article: Nuclear Receptor Signaling (2003) 1, e007 ...
Microarray Excel Hands-on Workshop Handout
Microarray Excel Hands-on Workshop Handout

... then calculated from a comparison of the d values from different permutations and a number of false significant genes (the false discovery rate) is calculated for each delta value. Detailed information about the methods and algorithms used in SAM can be found in the manual located at: C:\Program Fil ...
Gene regulation_1130(final)
Gene regulation_1130(final)

... • Insulin affects transcription, mRNA stability, and translation (from gene to protein) • Focusing on insulin-regulated gene transcription – Positive and negative effects on the transcription of specific genes even within the same cell. – Insulin-regulated genes  coding proteins involved in a varie ...
File - MMS Homework Helpers
File - MMS Homework Helpers

... studied peas because they were easy to grow and because they have many traits that exist only in two forms. He started his experiments with purebred plants. A purebred plant is one that always produces offspring with the same form of a trait as the parent. Because of the results of his experiments, ...
JUNGLE IGUANA ( Green ) well adapted to heavy tropical forest
JUNGLE IGUANA ( Green ) well adapted to heavy tropical forest

... new gene causes glands in the nose to collect extra salt from the blood and to expel that salt. • 3. “B” mutation of skin pigment gene causes black pigment instead of green • 4. “S” Mutation of foot shape gene on chromosome 2 causes webbed feet • 5. “C” The “calm gene” is a Mutation of the fear reac ...
Offspring Color for Toothpick Fish Generations
Offspring Color for Toothpick Fish Generations

... recessive, and which are equal (co-dominant). Skin color has a huge impact on how an animal lives, or ultimately dies. If an animal is bright in color, but their environment is dull colors, then that fish is less likely to survive. On the other hand, if a fish blends in with its environment, it has ...
Nucline RNA and Its Uses
Nucline RNA and Its Uses

... • It is conditionally translated into a protein. • Protein is only expressed when researcher-defined gene profiles are present in the cell. • It can be used to modify, tag, and even destroy cells that express the gene profile. • It is not siRNA (methylates target DNA) or antisense (blocks mRNA trans ...
Genetic Contribution to Coronary Atherosclerosis
Genetic Contribution to Coronary Atherosclerosis

... Making sense of a growing list of candidate genes Based on concepts derived from the study of accessible complex traits in different organisms, insight into genetic paradigms can be deduced from the analysis of clinical features of coronary artery disease. A priori, these paradigms need to be identi ...
mendel-test-AP-gibbs..
mendel-test-AP-gibbs..

... X-linked traits are said to be passed from father to daughter to grandson because _____. daughters never get the trait from their mother if a mother carries the allele, she must give it to her sons fathers must give their X chromosome with the allele to their daughters, and sons must get their X chr ...
Diagnostic and Prognostic Significance of Gene Expression
Diagnostic and Prognostic Significance of Gene Expression

... • Proto-oncogene located at 18q21 that promotes B-cell survival via inhibition of apoptosis and confers chemotherapy resistance • BCL-2 expression is normally down-regulated in the GC where apoptosis plays a critical role in negative B-cell selection • t(14;18)  fusion gene leading to transcription ...
What are Math and Computer Science doing in Biology?
What are Math and Computer Science doing in Biology?

... platelet-derived growth factor are derived from the same or closely related cellular genes. This conclusion is based on the demonstration of extensive sequence similarity.” From the abstract ...
Goldmine: Integrating information to place sets of genomic ranges
Goldmine: Integrating information to place sets of genomic ranges

... genomic ranges from any source can be loaded, for example, from BED files. Here we show an example of loading data from the Roadmap Epigenomics Project. In this example, we are interested in how DMRs may overlap and interact with known regulatory regions. The Roadmap Epigenomics Project produced uni ...
pEGFP-N1 - ResearchGate
pEGFP-N1 - ResearchGate

... Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo . The target gene should be cloned into pEGFP-N1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The inse ...
PPT - Bioinformatics.ca
PPT - Bioinformatics.ca

... • Genome-Scale Analysis (Omics) – Genomics, Proteomics ...
Slide 1
Slide 1

... – Mendel showed that parents pass heritable factors to offspring (heritable factors are now called genes) – Advantages of using pea plants ...
Genetics - Montville.net
Genetics - Montville.net

... mark or alter the hereditary makeup of an unborn child. 3. Color blindness is more common in males than in females. 4. A person may transmit characteristics to offspring which he/she does not show. 5. Certain inherited traits may be altered by the stars, planets or moon early in development. 6. The ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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