INHERITANCE OF POLYDACTYLY IN THE MOUSE
... of polydactyls are as follows: 75.6f 2.4 for litters born when dams (and sires) one to 4 months of age; 77.3 A 1.9, for 5 to 7 months of age; and 75.0 A 3.6, for 8 to 11 months of age. Matings in this line usually were not continued to the reproductive limit since the practice has been to discontinu ...
... of polydactyls are as follows: 75.6f 2.4 for litters born when dams (and sires) one to 4 months of age; 77.3 A 1.9, for 5 to 7 months of age; and 75.0 A 3.6, for 8 to 11 months of age. Matings in this line usually were not continued to the reproductive limit since the practice has been to discontinu ...
RNA interference - Bio
... stretches of > 4 T's or A's in the target sequence when designing sequences to be expressed from an RNA pol III promoter. Since some regions of mRNA may be either highly structured or bound by regulatory proteins, we generally select siRNA target sites at different positions along the length of th ...
... stretches of > 4 T's or A's in the target sequence when designing sequences to be expressed from an RNA pol III promoter. Since some regions of mRNA may be either highly structured or bound by regulatory proteins, we generally select siRNA target sites at different positions along the length of th ...
Methicillin-resistant Staphylococcus aureus (MRSA) Isolated from
... to synthesize different kinds of enterotoxins alone or in association, providing further evidence that MRSA may also be involved in food poisoning outbreaks. The identification of staphylococcal toxin A gene in S. aureus strains by PCR offers a very specific, sensitive and inexpensive alternative to ...
... to synthesize different kinds of enterotoxins alone or in association, providing further evidence that MRSA may also be involved in food poisoning outbreaks. The identification of staphylococcal toxin A gene in S. aureus strains by PCR offers a very specific, sensitive and inexpensive alternative to ...
PDF
... formation in Foxe3 mutants might be the altered expression of ␣A-crystallin. In the wild-type lens, lens fiber cells express high levels of crystallins, which can represent more than 90% of the total protein in the cell. ␣A-crystallin constitutes about 17% of the total protein in the cell. Studies i ...
... formation in Foxe3 mutants might be the altered expression of ␣A-crystallin. In the wild-type lens, lens fiber cells express high levels of crystallins, which can represent more than 90% of the total protein in the cell. ␣A-crystallin constitutes about 17% of the total protein in the cell. Studies i ...
Endoderm development in vertebrates: fate mapping
... as assessed by the early endoderm marker genes sox17, a high-mobility-group (HMG) transcription factor gene (Alexander & Stainier 1999) and foxA2, a winged helix/forkhead transcription factor gene (formerly known as axial (Strähle et al. 1993)) (Kikuchi et al. 2000; Reiter et al. 2001). These geneti ...
... as assessed by the early endoderm marker genes sox17, a high-mobility-group (HMG) transcription factor gene (Alexander & Stainier 1999) and foxA2, a winged helix/forkhead transcription factor gene (formerly known as axial (Strähle et al. 1993)) (Kikuchi et al. 2000; Reiter et al. 2001). These geneti ...
Molecular characterization of glutathione peroxidase
... (Promega, USA). Reverse transcription was performed with oligo(dT)18 primer using First Strand cDNA Synthesis Kit (Toyobo, Japan). Two degenerate primers GPx01F (5’-GGACATCAGGA GAACTGCAA(A/G)AA(T/C)GA(A/G)GA-3’) and GPx02R (5’-A CCAGGAACTT(C/T)TC(G/A)AA(G/A)TTCCA-3’) were designed to clone partial G ...
... (Promega, USA). Reverse transcription was performed with oligo(dT)18 primer using First Strand cDNA Synthesis Kit (Toyobo, Japan). Two degenerate primers GPx01F (5’-GGACATCAGGA GAACTGCAA(A/G)AA(T/C)GA(A/G)GA-3’) and GPx02R (5’-A CCAGGAACTT(C/T)TC(G/A)AA(G/A)TTCCA-3’) were designed to clone partial G ...
The EGF-CFC Protein One-Eyed Pinhead Is Essential for
... proteins have remained elusive. In cell culture assays, soluble Cripto protein induces responses such as Ras and MAPK activation (reviewed in Salomon et al., 1999), and in a yeast phosphorylation assay, FRL1 can activate the FGF receptor (Kinoshita et al., 1995). As no direct interaction of Cripto o ...
... proteins have remained elusive. In cell culture assays, soluble Cripto protein induces responses such as Ras and MAPK activation (reviewed in Salomon et al., 1999), and in a yeast phosphorylation assay, FRL1 can activate the FGF receptor (Kinoshita et al., 1995). As no direct interaction of Cripto o ...
factor involved in dorsal-ventral axis formation and neurogenesis
... solid boxes numbered 1-6 represent the exons from cDNAs encoding the putative spi protein; the hatched region within exon 6 represents the coding sequence (see also Fig. 3). All of the cDNAs examined that encode spi are composed of two or more exons. The location of exon 1 in the genomic region has ...
... solid boxes numbered 1-6 represent the exons from cDNAs encoding the putative spi protein; the hatched region within exon 6 represents the coding sequence (see also Fig. 3). All of the cDNAs examined that encode spi are composed of two or more exons. The location of exon 1 in the genomic region has ...
the hemophilia gene, click here
... can be passed from generation to generation. In about 3 out of 10 cases, however, a boy with hemophilia (or a girl who is a carrier of hemophilia) is born to a family that has no history of the disease. There are three possible explanations for this. 1. It could be that hemophilia was in the family ...
... can be passed from generation to generation. In about 3 out of 10 cases, however, a boy with hemophilia (or a girl who is a carrier of hemophilia) is born to a family that has no history of the disease. There are three possible explanations for this. 1. It could be that hemophilia was in the family ...
The Genetic Principles of Crossbreeding
... Crossbreeding is the mating of two individuals with different breed makeups. It is widely used in commercial beef production because of the benefits it has to offer cow-calf producers. Improvements in efficiency can be dramatic if appropriate breed combinations are used. Crossbreeding does not elimi ...
... Crossbreeding is the mating of two individuals with different breed makeups. It is widely used in commercial beef production because of the benefits it has to offer cow-calf producers. Improvements in efficiency can be dramatic if appropriate breed combinations are used. Crossbreeding does not elimi ...
Genetic diversity and epidemiology of infectious hematopoietic
... have a common viral ancestral lineage. Comparisons of multiple isolates from the same site provided epidemiological insights into viral transmission patterns and indicated that viral evolution, viral introduction, and genetic stasis were the mechanisms involved with IHN virus population dynamics in ...
... have a common viral ancestral lineage. Comparisons of multiple isolates from the same site provided epidemiological insights into viral transmission patterns and indicated that viral evolution, viral introduction, and genetic stasis were the mechanisms involved with IHN virus population dynamics in ...
Nucleotide sequence and taxonomic value of the
... A phage DNA production and cloning of the C. pneumoniae IOL-207 MOMP gene into LZAP II. Using E. coli 4358 as host, an amplified preparation of a single hybridizing recombinant plaque was used for the large-scale production (Sambrook er al., 1989) of 1 phage DNA. DNA was purified and concentrated wi ...
... A phage DNA production and cloning of the C. pneumoniae IOL-207 MOMP gene into LZAP II. Using E. coli 4358 as host, an amplified preparation of a single hybridizing recombinant plaque was used for the large-scale production (Sambrook er al., 1989) of 1 phage DNA. DNA was purified and concentrated wi ...
Molecular cloning of a laccase isozyme gene from
... inducible promoter. The native Ple. sajor-caju laccase signal sequence was effective in directing the secretion of lac4 expressed in Pic. pastoris. The control of media pH and temperature was found to be important in obtaining sufficient quantities of the protein to allow purification and subsequent ...
... inducible promoter. The native Ple. sajor-caju laccase signal sequence was effective in directing the secretion of lac4 expressed in Pic. pastoris. The control of media pH and temperature was found to be important in obtaining sufficient quantities of the protein to allow purification and subsequent ...
HS-SCI-APB-Unit 3 -- Chapter 14- Mendel and the
... In 1843, at the age of 21, Mendel entered an Augustinian monastery, a reasonable choice at that time for someone who valued the life of the mind. He considered becoming a teacher but failed the necessary examination. In 1851, he left the monastery to pursue two years of study in physics and chemistr ...
... In 1843, at the age of 21, Mendel entered an Augustinian monastery, a reasonable choice at that time for someone who valued the life of the mind. He considered becoming a teacher but failed the necessary examination. In 1851, he left the monastery to pursue two years of study in physics and chemistr ...
Cerberus regulates left–right asymmetry of the embryonic head and
... embryos with reversed hearts, 9 survived to later stages and the heads of all of them had turned to the left side (normally to the right). Mock-transfected cells (n = 42) and cells expressing cCer (n = 44) were also implanted on the left side of embryos. In this case, no difference in heart looping ...
... embryos with reversed hearts, 9 survived to later stages and the heads of all of them had turned to the left side (normally to the right). Mock-transfected cells (n = 42) and cells expressing cCer (n = 44) were also implanted on the left side of embryos. In this case, no difference in heart looping ...
genomebiology.com
... results indicate that only a very small portion of introns in invertebrates contain TEs (2 to 5%) compared to 20 to 40% of introns in non-mammalian vertebrates and approximately 60% in mammals. We also examined the average length of introns containing TEs. In C. elegans the median length of an intro ...
... results indicate that only a very small portion of introns in invertebrates contain TEs (2 to 5%) compared to 20 to 40% of introns in non-mammalian vertebrates and approximately 60% in mammals. We also examined the average length of introns containing TEs. In C. elegans the median length of an intro ...
Editorial Noncoding RNAs
... development and health in many ways. Another distinct and rapidly growing class of noncoding RNA, the long ncRNAs (lncRNAs), is also implicated in gene regulation and disease. Across the phylogenetic kingdoms, bacteria and other prokaryotes have proportionally the least amount of noncoding DNA. Inte ...
... development and health in many ways. Another distinct and rapidly growing class of noncoding RNA, the long ncRNAs (lncRNAs), is also implicated in gene regulation and disease. Across the phylogenetic kingdoms, bacteria and other prokaryotes have proportionally the least amount of noncoding DNA. Inte ...
Azza Ahmed Ibrahim Abo senna_GST paper
... leukemia has revealed a great number of non-random chromosome abnormalities. In many instances, molecular studies of these abnormalities identified specific genes implicated in the process of leukemogenesis (Mrozek et al., 2004) The environmental causes of acute leukemia, which have increased in the ...
... leukemia has revealed a great number of non-random chromosome abnormalities. In many instances, molecular studies of these abnormalities identified specific genes implicated in the process of leukemogenesis (Mrozek et al., 2004) The environmental causes of acute leukemia, which have increased in the ...
Complex Heterozygosity Screening with Actin Alanine Scan Alleles
... (better known as dominant enhancers) (Raftery et al. 1995), worms, and yeast (also known as unlinked non-complementation) (Stearns and Botstein 1988; Welch et al. 1993) but this genetic interaction space has been understudied and undersampled. From a genome-wide screen in yeast, we examined a deleti ...
... (better known as dominant enhancers) (Raftery et al. 1995), worms, and yeast (also known as unlinked non-complementation) (Stearns and Botstein 1988; Welch et al. 1993) but this genetic interaction space has been understudied and undersampled. From a genome-wide screen in yeast, we examined a deleti ...
Molecular and Genetic Characterization of a
... In this paper we describe a novel, dominant pleiotropic tomato (Lycopersicon esculentum)-ripening mutation, Cnr (colorless nonripening). This mutant occurred spontaneously in a commercial population. Cnr has a phenotype that is quite distinct from that of the other pleiotropic tomato-ripening mutant ...
... In this paper we describe a novel, dominant pleiotropic tomato (Lycopersicon esculentum)-ripening mutation, Cnr (colorless nonripening). This mutant occurred spontaneously in a commercial population. Cnr has a phenotype that is quite distinct from that of the other pleiotropic tomato-ripening mutant ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.