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light control of seedling development
light control of seedling development

... Perhaps the most important component for encoding the complexity of responses is the multiple families of photoreceptors. Seedling responses to light are mediated by at least three classes of regulatory photoreceptors: (a) phytochromes, which respond mainly to red and far-red light but which also ab ...
University of Groningen Lactococcus lactis systems biology Eckhardt
University of Groningen Lactococcus lactis systems biology Eckhardt

... Transcriptomics is a technology used in molecular biology research to uncover expression profiles of all genes in a specific cell type or in (sub)populations of cells at a given moment in time. The gene and promoter activity is expressed as the number of transcripts or messenger RNAs (mRNAs). With t ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic

... model organisms as well (Kaur et al. 1997; Walther and Wendland 2008; Wendland et al. 2000). Due to the standardized design of the pRS series, a single pair of oligonucleotide primers can be used to amplify any prototrophic marker from any pRS plasmid (Brachmann et al. 1998) for transforming yeast. ...
Useful Information for Lay People
Useful Information for Lay People

... person walks, talks or writes. Trying to balance and control their body can make them very tired. As Friedreichs Ataxia is progressive the symptoms get worse as time goes on; this is usually a gradual process, and takes place over a number of years and eventually it may be necessary to use a wheelch ...
Isolation of COV1, a gene involved in the regulation of vascular
Isolation of COV1, a gene involved in the regulation of vascular

... autonomous formation of veins in an initially uniform field. One of these substances promotes the induction of vascular tissue, whereas the other results in inhibition of vascular development (Koch and Meinhardt, 1994). Although there is a good deal of evidence to support the inductive effects of au ...
VCS: Tool for Visualizing Copy Number Variation and Single Nucleotide Polymorphism
VCS: Tool for Visualizing Copy Number Variation and Single Nucleotide Polymorphism

... understanding complex phenotypes or deseases susceptibility. Although thousands of CNVs and SNPs are currently avaliable in the public databases, they are somewhat difficult to use for analyses without visualization tools. We developed a web-based tool called the VCS (visualization of CNV or SNP) to ...
Gene Section SRSF1 (serine/arginine rich splicing factor 1) -
Gene Section SRSF1 (serine/arginine rich splicing factor 1) -

Comparative sequence analysis of the long repeat regions and
Comparative sequence analysis of the long repeat regions and

PDF
PDF

... SUMMARY In insects, the precise timing of molting and metamorphosis is strictly guided by a principal steroid hormone, ecdysone. Among the multiple conversion steps for synthesizing ecdysone from dietary cholesterol, the conversion of 7-dehydrocholesterol to 5ketodiol, the so-called ‘Black Box’, is ...
Inverse correlation between SMN1 and SMN2 copy numbers
Inverse correlation between SMN1 and SMN2 copy numbers

... two copies of SMN1 exon 7), 3.27  10 2; and ‘1D allele’ (chromosome 5 with a small intragenic mutation in SMN1), 1.80  10 4. One hypothesis to explain the presence of two copies of SMN1 on one chromosome 5 is unequal crossing over between homologous chromosomes during meiosis. Because of the prese ...
Understanding the pathological manifestations of aromatase excess
Understanding the pathological manifestations of aromatase excess

... Figure 4. Schematic representations of DNA recombination events that give rise to gain-of-function mutations in familial aromatase excess syndrome. CYP19A1 exons II–X are expressed in one box. The arrows and following closed boxes represent one of multiple CYP19A1 promoters. The arrows indicate tran ...
The Effect of OmpA Expression on Hfr Conjugation Efficiency
The Effect of OmpA Expression on Hfr Conjugation Efficiency

... addition to proline may be present. However, based on the location of proC on the genome with respect to the HfrC origin of transfer, the transfer direction, and the recipient strains Pro- phenotype, proline was deemed the most likely selective marker in our experiment. The HfrC origin lies at 96.80 ...
Programmed Ribosomal Frameshifting Generates a Copper
Programmed Ribosomal Frameshifting Generates a Copper

... the N-terminal segment of CopA proposed to serve as a transporter-linked copper chaperone in E. coli (Drees et al., 2015). In order to explore whether the N-terminal segment of CopA is indeed synthesized in the cell as an individual polypeptide, we used the copA-containing ASKA library plasmid (Kita ...
Small Nucleolar RNA
Small Nucleolar RNA

... snoRNAs can be used as a biological tool • (1) snoRNA localization elements have been utilized to direct new RNA sequences to the nucleolus • (2) snoRNAs have been used to direct nucleotide modifications to new sites in rRNA via the expression of snoRNAs containing novel guide sequences ...
Exon skipping and reading through stop codons
Exon skipping and reading through stop codons

PH4 of Petunia Is an R2R3 MYB Protein That Activates
PH4 of Petunia Is an R2R3 MYB Protein That Activates

... factor and a WD40 protein, respectively (de Vetten et al., 1997; Spelt et al., 2000). AN2 encodes a MYB-type transcription factor whose function appears to be (partially) redundant, because it is expressed only in petals and not in other pigmented tissues (Quattrocchio et al., 1999). Moreover, even ...
Evolution, structure and function of mitochondrial carriers: a
Evolution, structure and function of mitochondrial carriers: a

... (Palmieri et al., 2006 and references in this work), a third in H. sapiens (Palmieri, 2004 and references in this work) and a quarter in A. thaliana (Picault et al., 2004 and references in this work) were identified. Table 1 lists the main subfamilies into which MCs can be divided according to their ...
shows altered stratification and cell polarity
shows altered stratification and cell polarity

... cells of the apical ectodermal ridge (AER), a specialized region of the ectoderm at the Dorsal–Ventral (Do–Ve) margin of the developing limb (13). Evidence has been collected that, starting ∼E10.5–E11 the expression of several AER markers is lost in the central wedge of the AER, and at about the sam ...
Histological classification and molecular genetics of meningiomas
Histological classification and molecular genetics of meningiomas

1 Article: Investigation Evidence for Stabilizing Selection on Codon
1 Article: Investigation Evidence for Stabilizing Selection on Codon

... mutations. This gene arrangement polymorphism has been stable in natural populations for over seventy years (Figure 1A; Dobzhansky and Sturtevant 1938). Frequencies of particular chromosome arrangements form a classical cline across the species range in the American southwest despite the predicted e ...
A Single Eubacterial Origin of Eukaryotic
A Single Eubacterial Origin of Eukaryotic

... for bringing these sequences together in subsequent phylogenetic analyses. Because we observed moderate base composition heterogeneity at codon positions 1 and 2, we used LogDet/Paralinear distances, a phylogenetic method in PAUP*4.1b (Swofford 1998) which performs well in simulations even when base ...
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene

... ABSTRACT : The aim of the experiment was to detect polymorphisms in the keratin-associated protein 8.2 (KAP8.2) gene to determine associations between the genotype and fibre traits in Chinese Inner Mongolia cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashm ...
Effect of the allelic variants of aldehyde
Effect of the allelic variants of aldehyde

... The human ALDH superfamily comprises ten families, mapped to 11 chromosomes.14,15 Amino acid sequence identities are approximately 40 per cent between families and approximately 60 per cent or higher between subfamilies. The superfamily contains divergently related enzymes that metabolise a wide spe ...
Transgenic Tobacco Over-Expressing a Homeobox Gene Shows a
Transgenic Tobacco Over-Expressing a Homeobox Gene Shows a

... between the SAM and the leaf primordia. For example, it has been predicted that the shoot apex plays an important role in the acquisition of dorsoventral character by leaf primordia (Wardlaw 1949, Sussex 1955, Snow and Snow 1959). The arrangement of leaves along the stem, termed phyllotaxy, is stric ...
Kein Folientitel
Kein Folientitel

... zygote ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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