JunctionSeq Package User Manual
... Under the default parameterization, JunctionSeq also builds upon and expands the basic design put forth by DEXSeq, providing (among other things) the ability to test for both differential exon usage and differential splice junction usage. These two types of analyses are complementary: Exons represen ...
... Under the default parameterization, JunctionSeq also builds upon and expands the basic design put forth by DEXSeq, providing (among other things) the ability to test for both differential exon usage and differential splice junction usage. These two types of analyses are complementary: Exons represen ...
XistAR write up
... cells). Since it is impossible to deduce allele-specific expression in random inactivation cell lines, they utilized F1 hybrids that are Tsix-heterozygous. In these hybrids, X inactivation would be in favor the X-chromosome with mutant Tsix(XΔTsix). Again, XistAR was detected only from inactivated X ...
... cells). Since it is impossible to deduce allele-specific expression in random inactivation cell lines, they utilized F1 hybrids that are Tsix-heterozygous. In these hybrids, X inactivation would be in favor the X-chromosome with mutant Tsix(XΔTsix). Again, XistAR was detected only from inactivated X ...
powerpoint - McGill University
... Effect of the ivermectin (IVE) and selamectin (SEL)] on mitoxantrone (MXR) accumulation mediated by human ABCG2 and murine Abcg2. Transduced MDCKII cells were preincubated with or without Ko143 (1 µM) or the other tested compounds (50 µM). Mean MXR fluorescence is shown in terms of relative arbitrar ...
... Effect of the ivermectin (IVE) and selamectin (SEL)] on mitoxantrone (MXR) accumulation mediated by human ABCG2 and murine Abcg2. Transduced MDCKII cells were preincubated with or without Ko143 (1 µM) or the other tested compounds (50 µM). Mean MXR fluorescence is shown in terms of relative arbitrar ...
15_Lecture_Presentation
... Morgan’s Experimental Evidence: Scientific Inquiry • The first solid evidence associating a specific gene with a specific chromosome came from Thomas Hunt Morgan, an embryologist • Morgan’s experiments with fruit flies provided convincing evidence that chromosomes are the location of Mendel’s herit ...
... Morgan’s Experimental Evidence: Scientific Inquiry • The first solid evidence associating a specific gene with a specific chromosome came from Thomas Hunt Morgan, an embryologist • Morgan’s experiments with fruit flies provided convincing evidence that chromosomes are the location of Mendel’s herit ...
Significant enhancement of fatty acid composition in seeds of the
... 2010; Kang et al., 2011; Nguyen et al., 2013)—share strong homology with each other, we designed sgRNA constructs for use in Camelina but tested them first in Arabidopsis to determine their efficacy in creating Cas9/sgRNA-mediated gene mutations and changing the fatty acid composition of seeds. This ...
... 2010; Kang et al., 2011; Nguyen et al., 2013)—share strong homology with each other, we designed sgRNA constructs for use in Camelina but tested them first in Arabidopsis to determine their efficacy in creating Cas9/sgRNA-mediated gene mutations and changing the fatty acid composition of seeds. This ...
Cell biology of carbohydrate metabolism
... cells; lane 2, extract from 293 cells transfected with HA-tagged Mlx and FLAG-tagged ChREBP; lane 3, same as lane 2 except incubated with antibodies to HA; lane 4, same as lane 2 except incubated with antibodies to FLAG. The arrow indicates the position of the ChREBP·Mlx complex. ...
... cells; lane 2, extract from 293 cells transfected with HA-tagged Mlx and FLAG-tagged ChREBP; lane 3, same as lane 2 except incubated with antibodies to HA; lane 4, same as lane 2 except incubated with antibodies to FLAG. The arrow indicates the position of the ChREBP·Mlx complex. ...
v7a29-zhu pgmkr - Molecular Vision
... We have cloned and characterized Xenopus cry homologs with high sequence similarity to the mammalian crys. However, in contrast to the two cry genes found in both mouse and human, we identified three distinct clones from the Xenopus retinal cDNA library. One of the clones, xcry1, is most similar to ...
... We have cloned and characterized Xenopus cry homologs with high sequence similarity to the mammalian crys. However, in contrast to the two cry genes found in both mouse and human, we identified three distinct clones from the Xenopus retinal cDNA library. One of the clones, xcry1, is most similar to ...
2. Assuming homozygosity for the normal gene, the mating is A/A · b
... pure–breeding stock and that a cross of two platinum foxes results in some normal progeny. Platinum must be dominant to normal color and heterozygous (A/a). An 82:38 ratio is very close to 2:1. Because a 1:2:1 ratio is expected in a heterozygous cross, one genotype is nonviable. It must be the A/A, ...
... pure–breeding stock and that a cross of two platinum foxes results in some normal progeny. Platinum must be dominant to normal color and heterozygous (A/a). An 82:38 ratio is very close to 2:1. Because a 1:2:1 ratio is expected in a heterozygous cross, one genotype is nonviable. It must be the A/A, ...
ASCO_2009_files/Blanke GIST PD ASCO2009
... signatures and response on RTOG 0132: Background and Importance • 01321: Phase II neoadjuvant trial imatinib in KIT + potentially resectable GIST (primary or metastatic) ...
... signatures and response on RTOG 0132: Background and Importance • 01321: Phase II neoadjuvant trial imatinib in KIT + potentially resectable GIST (primary or metastatic) ...
incomplete dominance - Gulf Coast State College
... • When just one dominant gene is present, the trait will be shown • Two dominant alleles does not enhance the trait • For a recessive trait to be expressed both parents MUST pass on the recessive allele for this trait to be present • Which genotype would be the carrier for the recessive trait? ...
... • When just one dominant gene is present, the trait will be shown • Two dominant alleles does not enhance the trait • For a recessive trait to be expressed both parents MUST pass on the recessive allele for this trait to be present • Which genotype would be the carrier for the recessive trait? ...
Applicability of Yeast Genetics to Neurologic Disease
... s advances in gene mapping technology reveal genes associated with neurologic diseases, the need to identify a gene’s normal function arises often. Experimental genetics is very useful in identifying a gene’s function. It relies on model organisms both because it is not appropriate in humans, and be ...
... s advances in gene mapping technology reveal genes associated with neurologic diseases, the need to identify a gene’s normal function arises often. Experimental genetics is very useful in identifying a gene’s function. It relies on model organisms both because it is not appropriate in humans, and be ...
Multiple Choice - Test Bank Team
... Which of the following statements is confirmed by the Western blot shown? A. SWR1 deposits H2AZ histones into the nucleosome arrays. B. SWR1 function is not ATP-dependent. C. The antibody used in this experiment binds to the SWR1 complex. D. All of the above. ...
... Which of the following statements is confirmed by the Western blot shown? A. SWR1 deposits H2AZ histones into the nucleosome arrays. B. SWR1 function is not ATP-dependent. C. The antibody used in this experiment binds to the SWR1 complex. D. All of the above. ...
Slide 1
... 4. Law of segregation: Allele pairs separate (segregate) from each other during the production of gametes so that a sperm or egg carries only one allele for each gene ...
... 4. Law of segregation: Allele pairs separate (segregate) from each other during the production of gametes so that a sperm or egg carries only one allele for each gene ...
`Candidatus Midichloria mitochondrii`, an endosymbiont of the tick
... generated using MrBayes. The GenBank accession number for each sequence is indicated. Numbers adjacent to each node represent the posterior probability values. Acetobacter aceti (Rhodospirillales) was included as an outgroup. Additional analyses in which other Alphaproteobacteria were included as ou ...
... generated using MrBayes. The GenBank accession number for each sequence is indicated. Numbers adjacent to each node represent the posterior probability values. Acetobacter aceti (Rhodospirillales) was included as an outgroup. Additional analyses in which other Alphaproteobacteria were included as ou ...
Key transcriptional regulators of the vasoprotective effects of shear
... stress exposure in vivo. Nonetheless, shortterm shear stress exposure gives vital clues about the mechanisms by which endothelial cells sense shear stress and convey these signals. For example, using shear stress exposure of less than one hour, Tzima and colleagues identified a cell-cell junctional ...
... stress exposure in vivo. Nonetheless, shortterm shear stress exposure gives vital clues about the mechanisms by which endothelial cells sense shear stress and convey these signals. For example, using shear stress exposure of less than one hour, Tzima and colleagues identified a cell-cell junctional ...
biol b242 chromosomal evolution
... Because the genes are arranged on long strings, and because chromosomes themselves act as genetic elements:There may be holistic selective effects that act on 100s to 1000s of genes at a time. Evolutionary oddities about chromosomes Although we understand some of the processes involved in chromosoma ...
... Because the genes are arranged on long strings, and because chromosomes themselves act as genetic elements:There may be holistic selective effects that act on 100s to 1000s of genes at a time. Evolutionary oddities about chromosomes Although we understand some of the processes involved in chromosoma ...
Ключові слова - Буковинський державний медичний університет
... In patients with alcoholic hepatitis eNOS gene expression does not change, but a decrease in the enzymatic activity of eNOS is observed as a result of the inhibitory effect of caveolin-1 and regulatory protein NOSTRIN, which concentration increases in the blood of such patients [27]. Increased intra ...
... In patients with alcoholic hepatitis eNOS gene expression does not change, but a decrease in the enzymatic activity of eNOS is observed as a result of the inhibitory effect of caveolin-1 and regulatory protein NOSTRIN, which concentration increases in the blood of such patients [27]. Increased intra ...
Wolfinger Russ - MCP Conference 2015
... • Biologists, chemists, regulators concerned with lack of reproducibility of significant gene lists, and have observed that lists based on fold change (FC) are more consistent than those based on p-values (P) • Statisticians usually seek an optimal tradeoff between specificity (Type 1) and sensitivi ...
... • Biologists, chemists, regulators concerned with lack of reproducibility of significant gene lists, and have observed that lists based on fold change (FC) are more consistent than those based on p-values (P) • Statisticians usually seek an optimal tradeoff between specificity (Type 1) and sensitivi ...
Molecular regulators of phosphate homeostasis in plants
... supply is inadequate. Over the past decade, significant progress has been made toward understanding such regulation at the molecular level. In this review, the focus is on the molecular regulators that mediate cellular Pi concentrations. The regulators are introduced and organized according to their ...
... supply is inadequate. Over the past decade, significant progress has been made toward understanding such regulation at the molecular level. In this review, the focus is on the molecular regulators that mediate cellular Pi concentrations. The regulators are introduced and organized according to their ...
Modification of the terminal restriction fragment length polymorphism
... reverse labeled primers and compare the movement of the fragment lengths from both sides. The method we propose can also adjust the deviation at least for the comparison between the two or multiple profiles. The second most important influencing factor was found to be the differences in the amplific ...
... reverse labeled primers and compare the movement of the fragment lengths from both sides. The method we propose can also adjust the deviation at least for the comparison between the two or multiple profiles. The second most important influencing factor was found to be the differences in the amplific ...
High mutation rates in human and ape pseudoautosomal genes
... than elsewhere in the genome (May et al., 2002). A noncoding pseudoautosomal region close to the Xp/Yp telomere was reported to have a high substitution rate (Cooke et al., 1985; Baird and Royle, 1997), however, subtelomeric regions are known to evolve very fast, perhaps, due to ectopic recombinatio ...
... than elsewhere in the genome (May et al., 2002). A noncoding pseudoautosomal region close to the Xp/Yp telomere was reported to have a high substitution rate (Cooke et al., 1985; Baird and Royle, 1997), however, subtelomeric regions are known to evolve very fast, perhaps, due to ectopic recombinatio ...
Part 1: PCA & MDS COS 323
... PCA for DNA Microarrays • PCA shows patterns of correlated activation – Genes with same pattern might have similar function ...
... PCA for DNA Microarrays • PCA shows patterns of correlated activation – Genes with same pattern might have similar function ...
Isolation and Characterization of Rice MADS Box Gene
... C1032 (0sMADS5) was mapped on chromosome 6. The localization is consistent with the result of Kang et al.10 From this localization, we suspected that C1032 might be related to depressed palea-1 (dp-1),32 and did more precise mapping to determine the loci of dp-1 and C1032. Genomic DNA from 90 F 2 pl ...
... C1032 (0sMADS5) was mapped on chromosome 6. The localization is consistent with the result of Kang et al.10 From this localization, we suspected that C1032 might be related to depressed palea-1 (dp-1),32 and did more precise mapping to determine the loci of dp-1 and C1032. Genomic DNA from 90 F 2 pl ...
TissueNet database of human tissue protein–protein interactions
... Here, we focus on the human PPIs. Unlike unicellular organisms such as yeast, the human body is composed of many tissues, each expressing a distinct set of proteins [e.g. (8,9)]. Consequently, many proteins have varying PPI partners across tissues (10,11). A tissue-sensitive view of PPIs is therefor ...
... Here, we focus on the human PPIs. Unlike unicellular organisms such as yeast, the human body is composed of many tissues, each expressing a distinct set of proteins [e.g. (8,9)]. Consequently, many proteins have varying PPI partners across tissues (10,11). A tissue-sensitive view of PPIs is therefor ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.