The slime strain was grown in Nelson B medium... for 22 hours. HCH stereoisomers were dissolved in 83% ethanol...
... and F. oxysporum using medium containing chlorate, a toxic analog of nitrate (Klittich The clearest complementation reactions are and Leslie 1988 Genetics 118: in press). obtained when one nit mutant is deficient in a gene for production of a molybdenum cofactor (nitM) and the other nit mutant is de ...
... and F. oxysporum using medium containing chlorate, a toxic analog of nitrate (Klittich The clearest complementation reactions are and Leslie 1988 Genetics 118: in press). obtained when one nit mutant is deficient in a gene for production of a molybdenum cofactor (nitM) and the other nit mutant is de ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
... encoded amino acid sequence. We also removed three palindromic sequences which could spuriously affect expression (22bp, 18bp, and 16bp, each with one mismatch). iii) Codon usage. In general, codon usage presumably reflects the availability of tRNA isoforms in different organisms; efficiently expres ...
... encoded amino acid sequence. We also removed three palindromic sequences which could spuriously affect expression (22bp, 18bp, and 16bp, each with one mismatch). iii) Codon usage. In general, codon usage presumably reflects the availability of tRNA isoforms in different organisms; efficiently expres ...
Ectopic induction and reorganization of Wnt-1
... 1987), Xenopus (Noordermeer et al., 1989), and zebrafish (Molven et al., 1991)). In the mouse embryo, expression in this region is very dynamic (Wilkinson et al., 1987; Bally-Cuif et al., 1992; Paar et al., 1993): it starts at the one-somite stage as a broad domain encompassing what is probably most ...
... 1987), Xenopus (Noordermeer et al., 1989), and zebrafish (Molven et al., 1991)). In the mouse embryo, expression in this region is very dynamic (Wilkinson et al., 1987; Bally-Cuif et al., 1992; Paar et al., 1993): it starts at the one-somite stage as a broad domain encompassing what is probably most ...
X-LINKED DOMINANT INHERITANCE: mother affected
... the altered gene are affected in X-linked dominant disorders. In each cell of a female, either the paternally or maternally inherited X chromosome has been inactivated at random. This ensures that the concentrations of gene products from the X chromosome are the same as in a male. Females affected b ...
... the altered gene are affected in X-linked dominant disorders. In each cell of a female, either the paternally or maternally inherited X chromosome has been inactivated at random. This ensures that the concentrations of gene products from the X chromosome are the same as in a male. Females affected b ...
no correlation between cyp17-34t/c polymorphism and severe acne
... A total of 41 Indonesian individuals participated in the study; 21 of them were patients presenting with acne including 15 males and 6 females, and 20 were healthy individuals used as control group including 9 males and 11 females. All participants in the study gave their signed informed consent. Al ...
... A total of 41 Indonesian individuals participated in the study; 21 of them were patients presenting with acne including 15 males and 6 females, and 20 were healthy individuals used as control group including 9 males and 11 females. All participants in the study gave their signed informed consent. Al ...
Sequence requirements for function of the
... Large cloned fragments of the chorion gene loci can be re-introduced into the Drosophila genome via P elementmediated germline transformation, and these transgenic constructs can amplify with the correct tissue and temporal specificity (deCicco and Spradling, 1984). However, amplification is highly ...
... Large cloned fragments of the chorion gene loci can be re-introduced into the Drosophila genome via P elementmediated germline transformation, and these transgenic constructs can amplify with the correct tissue and temporal specificity (deCicco and Spradling, 1984). However, amplification is highly ...
Martina Šeruga Musić, Mladen Krajačić, Dijana Škorić
... technical simplicity, it is widely used as a screening tool in various investigations, ranging from clinical diagnosis of human hereditary diseases to the characterization of microbial communities. The technique is based on the fact that, after chemical and/or heat denaturing, ssDNA of different pri ...
... technical simplicity, it is widely used as a screening tool in various investigations, ranging from clinical diagnosis of human hereditary diseases to the characterization of microbial communities. The technique is based on the fact that, after chemical and/or heat denaturing, ssDNA of different pri ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... Complex, multisubunit, cut DNA at random, far from their recognition sequences. They may have biological significance but have a very little practical value since they do not produce discrete restriction fragments. ...
... Complex, multisubunit, cut DNA at random, far from their recognition sequences. They may have biological significance but have a very little practical value since they do not produce discrete restriction fragments. ...
Development 130, 3567-3578. - Howard Hughes Medical Institute
... allantois (Fig. 2D,D′) were observed. The rest of the mutants (46) were morphologically indistinguishable from their heterozygous and wild-type littermates. The phenotype of the four abnormal mutants was similar to, but less pronounced than, the ventralization of the mesoderm observed in double homo ...
... allantois (Fig. 2D,D′) were observed. The rest of the mutants (46) were morphologically indistinguishable from their heterozygous and wild-type littermates. The phenotype of the four abnormal mutants was similar to, but less pronounced than, the ventralization of the mesoderm observed in double homo ...
as PDF
... Amoebas that have 2 or more DP are replaced by two Foodstuff Cubes of each of the colors in play. In Descending Order, the players’ Score markers are moved based on the number of live amoebas they have on the board and the number of Gene Cards they possess. Advanced Genes count as 2 cards. Occupied ...
... Amoebas that have 2 or more DP are replaced by two Foodstuff Cubes of each of the colors in play. In Descending Order, the players’ Score markers are moved based on the number of live amoebas they have on the board and the number of Gene Cards they possess. Advanced Genes count as 2 cards. Occupied ...
Journal of Bacteriology
... 1B) show little if any nod gene-inducing activity when strain RBL5284, harboring the nodDI gene of R. meliloti, is used as an indicator. The other two indicator strains show significant background activity (Fig. 1B and 1C). Therefore, ,B-galactosidase production by strain RBL5284 can be used as a sp ...
... 1B) show little if any nod gene-inducing activity when strain RBL5284, harboring the nodDI gene of R. meliloti, is used as an indicator. The other two indicator strains show significant background activity (Fig. 1B and 1C). Therefore, ,B-galactosidase production by strain RBL5284 can be used as a sp ...
Hierarchical Text Categorization and Its Evaluation
... Suppressor of yeast mitochondrial ochre mutations that maps in or near the 15S ribosomal RNA gene of mtDNA. Fox TD, Staempfli S. A polypeptide chain-terminating mutation in the yeast mitochondrial oxi 1 gene has been shown to be an ochre (TAA) mutation by DNA sequence analysis. … ...
... Suppressor of yeast mitochondrial ochre mutations that maps in or near the 15S ribosomal RNA gene of mtDNA. Fox TD, Staempfli S. A polypeptide chain-terminating mutation in the yeast mitochondrial oxi 1 gene has been shown to be an ochre (TAA) mutation by DNA sequence analysis. … ...
fulltext - DiVA portal
... encodes a 34-kDa protein, ClyA (also referred to as HlyE and SheA) which causes lysis of mammalian cells by pore formation in a calcium-independent fashion. ClyA is the only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2- ...
... encodes a 34-kDa protein, ClyA (also referred to as HlyE and SheA) which causes lysis of mammalian cells by pore formation in a calcium-independent fashion. ClyA is the only cytolytic factor found in non-pathogenic strains of E. coli including the K-12 strains commonly used in laboratory studies [2- ...
Document
... BUT THIS IS INCORRECT! Probabilities should be multiplied only when they are completely independent of each other, and these are not. Developing HD is conditional on having inherited the gene. Have to use Bayes theorem:His mother’s probability of having gene for HD and asymptomatic at age 50 is 1/3. ...
... BUT THIS IS INCORRECT! Probabilities should be multiplied only when they are completely independent of each other, and these are not. Developing HD is conditional on having inherited the gene. Have to use Bayes theorem:His mother’s probability of having gene for HD and asymptomatic at age 50 is 1/3. ...
The presence of two UvrB subunits in the UvrAB complex ensures
... after isolation of UvrB±DNA complexes in the presence of ATP. This might be explained by assuming that the ATP in the UvrB±DNA complexes can be hydrolysed to ADP. Addition of ADP to UvrB±DNA complexes without a cofactor indeed does not restore DNA wrapping (Table II). If some of the complexes isolat ...
... after isolation of UvrB±DNA complexes in the presence of ATP. This might be explained by assuming that the ATP in the UvrB±DNA complexes can be hydrolysed to ADP. Addition of ADP to UvrB±DNA complexes without a cofactor indeed does not restore DNA wrapping (Table II). If some of the complexes isolat ...
Hemoglobin D Trait - West Virginia Department of Health and
... Your child does not have hemoglobin sickle D disease, but future children and other family members may be at risk for having it. When a person has hemoglobin sickle D disease, they do not inherit any of the usual hemoglobin A; they inherit a hemoglobin S gene (sickle hemoglobin) from one parent and ...
... Your child does not have hemoglobin sickle D disease, but future children and other family members may be at risk for having it. When a person has hemoglobin sickle D disease, they do not inherit any of the usual hemoglobin A; they inherit a hemoglobin S gene (sickle hemoglobin) from one parent and ...
The murine homologue of HIRA, a DiGeorge
... (Fig. 1A). Complete sequencing and assembly of selected clones allowed us to reconstitute a cDNA of 3852 bp (accession number EMBL X99712), containing an ORF of 3045 bp. The 1015 aa predicted protein has seven WD40 repeats (at aa positions 10–44, 68–98, 129–159, 172–202, 234–259, 266–313 and 326–356 ...
... (Fig. 1A). Complete sequencing and assembly of selected clones allowed us to reconstitute a cDNA of 3852 bp (accession number EMBL X99712), containing an ORF of 3045 bp. The 1015 aa predicted protein has seven WD40 repeats (at aa positions 10–44, 68–98, 129–159, 172–202, 234–259, 266–313 and 326–356 ...
From bedside to bench: how to analyze a splicing
... Another point to keep in mind when performing these types of experiments is the potential presence of allele specific polymorphisms. Minimal alterations in alternatively spliced products can result in disuse, and making sure that we look at any eventual effect in the mRNA splicing of the specific al ...
... Another point to keep in mind when performing these types of experiments is the potential presence of allele specific polymorphisms. Minimal alterations in alternatively spliced products can result in disuse, and making sure that we look at any eventual effect in the mRNA splicing of the specific al ...
(Japan), organized by Nori Satoh
... Developmental signaling by retinoic acid (RA) is thought to be an innovation essential for the origin of the chordate body plan. The larvacean urochordate Oikopleura dioica maintains a chordate body plan throughout life, and yet its genome appears to lack genes for RA synthesis, degradation, and rec ...
... Developmental signaling by retinoic acid (RA) is thought to be an innovation essential for the origin of the chordate body plan. The larvacean urochordate Oikopleura dioica maintains a chordate body plan throughout life, and yet its genome appears to lack genes for RA synthesis, degradation, and rec ...
CTLA-4 gene expression is influenced by promoter and
... (C−318/T−318), and adenine-guanine dimorphism in the exon 1 leader sequence at position 49 (A49/G49), an a multiallelic dinucleotide repeat in the 3′ untranslated region (UTR) of exon 4 (Figure 1). These three polymorphisms have been investigated for linkage and association in a number of human auto ...
... (C−318/T−318), and adenine-guanine dimorphism in the exon 1 leader sequence at position 49 (A49/G49), an a multiallelic dinucleotide repeat in the 3′ untranslated region (UTR) of exon 4 (Figure 1). These three polymorphisms have been investigated for linkage and association in a number of human auto ...
E.coli
... 1. The analysis of genome organization and the identification of genes, particularly in organisms with large genome sizes (human DNA is 3 109 bp, for example) is difficult to use plasmid and bacteriophage vectors, since the relatively small size capacity of these vectors for cloned DNA means tha ...
... 1. The analysis of genome organization and the identification of genes, particularly in organisms with large genome sizes (human DNA is 3 109 bp, for example) is difficult to use plasmid and bacteriophage vectors, since the relatively small size capacity of these vectors for cloned DNA means tha ...
The genetic basis of panic and phobic anxiety disorders
... the alleles studied include variants that are either directly related to the phenotype or strongly correlated with (i.e., in linkage disequilibrium with) such causal variants. Two approaches to association analysis are now feasible: (1) candidate gene studies, in which loci hypothesized to be causal ...
... the alleles studied include variants that are either directly related to the phenotype or strongly correlated with (i.e., in linkage disequilibrium with) such causal variants. Two approaches to association analysis are now feasible: (1) candidate gene studies, in which loci hypothesized to be causal ...
Special Report on Genetics and: Gout 23 Selected Articles From 199
... Our purpose is to provide both general and focused information regarding the interplay between genetics, health, and the environment, including but not limited to nutrition. Genetics matter, but in most cases your lifestyle and environment matter even more. It’s often said, “Genetics loads the gun, ...
... Our purpose is to provide both general and focused information regarding the interplay between genetics, health, and the environment, including but not limited to nutrition. Genetics matter, but in most cases your lifestyle and environment matter even more. It’s often said, “Genetics loads the gun, ...
Sugar and hormone connections - Department of Molecular Biology
... (ABA1, ABA2, ABA3, NCED3 and AAO3) has revealed more insights into the role of ABA and the regulatory mechanisms of ABA accumulation during sugar responses [19,23 – 26]. Interestingly, it was found that the transcript levels of several ABA biosynthesis genes are raised by glucose [19]. This inductio ...
... (ABA1, ABA2, ABA3, NCED3 and AAO3) has revealed more insights into the role of ABA and the regulatory mechanisms of ABA accumulation during sugar responses [19,23 – 26]. Interestingly, it was found that the transcript levels of several ABA biosynthesis genes are raised by glucose [19]. This inductio ...
recommended breeding policy for the maine
... Orange (O) This is a mutation on the X chromosome and is thus sex-linked. The gene eliminates all melanin pigment (black and brown) from the hair fibres, replacing it with phaenomelanin, a lighter compound appearing yellow or orange depending on the density of pigment granules. The O allele is also ...
... Orange (O) This is a mutation on the X chromosome and is thus sex-linked. The gene eliminates all melanin pigment (black and brown) from the hair fibres, replacing it with phaenomelanin, a lighter compound appearing yellow or orange depending on the density of pigment granules. The O allele is also ...