TaqMan-Based Real-Time PCR for Genotyping Common
... reported, one of which, HP Johnson, occurs at low frequency in various populations (3 ). The molecular weight of the ␣ chain is larger (23 kDa) than that encoded by HP2, and Southern blotting has shown 3 tandem repeats of the same 1.7-kb DNA segment found in the HP2 gene duplication (4 ). Another va ...
... reported, one of which, HP Johnson, occurs at low frequency in various populations (3 ). The molecular weight of the ␣ chain is larger (23 kDa) than that encoded by HP2, and Southern blotting has shown 3 tandem repeats of the same 1.7-kb DNA segment found in the HP2 gene duplication (4 ). Another va ...
Persistence and Loss of Meiotic Recombination
... The contradiction between the long-term persistence of the chromosomal hotspots that initiate meiotic recombination and the self-destructive mechanism by which they act strongly suggests that our understanding of recombination is incomplete. This “hotspot paradox” has been reinforced by the finding ...
... The contradiction between the long-term persistence of the chromosomal hotspots that initiate meiotic recombination and the self-destructive mechanism by which they act strongly suggests that our understanding of recombination is incomplete. This “hotspot paradox” has been reinforced by the finding ...
Functional characterisation of the TUP5 gene in - diss.fu
... 1 Summary Arginine is one of 20 standard amino acids necessary for the formation of peptides and proteins. It is also used as a nitrogen storage compound in seeds. Furthermore, arginine is the precursor of polyamines and nitric oxide which play an important role in many developmental processes and ...
... 1 Summary Arginine is one of 20 standard amino acids necessary for the formation of peptides and proteins. It is also used as a nitrogen storage compound in seeds. Furthermore, arginine is the precursor of polyamines and nitric oxide which play an important role in many developmental processes and ...
Gene replacement with the human BRCA1 locus
... Phillips et al., 1999), mice with mutations in Brca1 develop tumors which show allelic loss of Trp53 (Xu et al., 1999), and bigenic mice with mutations in both Brca1+/7 and Trp537/7 are speci®cally prone to the development of breast cancers (Cressman et al., 1999b; Xu et al., 1999). Paradoxically, h ...
... Phillips et al., 1999), mice with mutations in Brca1 develop tumors which show allelic loss of Trp53 (Xu et al., 1999), and bigenic mice with mutations in both Brca1+/7 and Trp537/7 are speci®cally prone to the development of breast cancers (Cressman et al., 1999b; Xu et al., 1999). Paradoxically, h ...
Scriver Charles R. Garrod`s Croonian Lectures (1908)
... Meanwhile, in medicine, the apparent rarity of the inborn errors of metabolism made them irrelevant to the medical profession and efforts to show their inheritance and congenital nature were of little help to the practice of medicine in Garrod_s lifetime. Moreover, the opinion that these disorders w ...
... Meanwhile, in medicine, the apparent rarity of the inborn errors of metabolism made them irrelevant to the medical profession and efforts to show their inheritance and congenital nature were of little help to the practice of medicine in Garrod_s lifetime. Moreover, the opinion that these disorders w ...
The Role of Carbohydrate Response Element Binding Protein in
... intestine, absorption rates for fructose are much slower than those for glucose [12]. In one study in humans, ingestion of 5 or 10 grams of fructose led to 10% of the study group being diagnosed as fructose malabsorbers [28]. This number increased to 40% when 20 grams of fructose was ingested [28]. ...
... intestine, absorption rates for fructose are much slower than those for glucose [12]. In one study in humans, ingestion of 5 or 10 grams of fructose led to 10% of the study group being diagnosed as fructose malabsorbers [28]. This number increased to 40% when 20 grams of fructose was ingested [28]. ...
Full Text
... implicated asymmetric sources of signals around Hensen’s node as the initial event leading to later L-R asymmetry of the viscera (reviewed (Harvey, 1998)). Recent research has identified molecular events upstream of the formation of the organizer, as well as many candidate signaling components for t ...
... implicated asymmetric sources of signals around Hensen’s node as the initial event leading to later L-R asymmetry of the viscera (reviewed (Harvey, 1998)). Recent research has identified molecular events upstream of the formation of the organizer, as well as many candidate signaling components for t ...
Functional Characterization of Nine Norway
... in Fäldt et al. (2003b). Combination of similarity-based PCR strategies previously developed for isolation of conifer TPS genes (Bohlmann et al., 1997), mining of expressed sequence tags, and RACE cloning strategies enabled the isolation of an additional five TPS cDNAs (PaTPS-Lon, PaTPS-Lim, PaTPS- ...
... in Fäldt et al. (2003b). Combination of similarity-based PCR strategies previously developed for isolation of conifer TPS genes (Bohlmann et al., 1997), mining of expressed sequence tags, and RACE cloning strategies enabled the isolation of an additional five TPS cDNAs (PaTPS-Lon, PaTPS-Lim, PaTPS- ...
Leukaemia Section 3q rearrangements in myeloid malignancies Atlas of Genetics and Cytogenetics
... Often associated with young age at diagnosis, trilineage dysplasia, dysmegakaryopoiesis and prior treatment with alkylating agents. ...
... Often associated with young age at diagnosis, trilineage dysplasia, dysmegakaryopoiesis and prior treatment with alkylating agents. ...
Inbreeding in Swine, nsif-fs4 - National Swine Improvement Federation
... Most breeds of livestock went through a phase of inbreeding early in their development. This resulted from the need to establish color patterns and other aspects of physical appearance. Since one of the results of inbreeding is to establish more genetic uniformity, those traits that have simple mean ...
... Most breeds of livestock went through a phase of inbreeding early in their development. This resulted from the need to establish color patterns and other aspects of physical appearance. Since one of the results of inbreeding is to establish more genetic uniformity, those traits that have simple mean ...
Comparative Sequence Analysis of the Genomic Segment 6 of Four
... sequence through the cloned cDNA. One artefact observed is premature termination (bands in all sequencing lanes); however, carrying out the reverse transcriptase reaction at 50 °C or using different primers helps to eliminate this problem. The gene 6 of three strains, 1076, Gottfried and FI-14, cont ...
... sequence through the cloned cDNA. One artefact observed is premature termination (bands in all sequencing lanes); however, carrying out the reverse transcriptase reaction at 50 °C or using different primers helps to eliminate this problem. The gene 6 of three strains, 1076, Gottfried and FI-14, cont ...
Gene Section MLL (myeloid/lymphoid or mixed lineage leukemia) Atlas of Genetics and Cytogenetics
... bind acetylated histones), and 1 plant homeodomain; these domains may be involved in protein-protein interaction; a FYRN and a FRYC domain; a transactivation domain which binds CBP; may acetylates H3 and H4 in the HOX area; a SET domain: methyltransferase; methyltates H3, including histones in the H ...
... bind acetylated histones), and 1 plant homeodomain; these domains may be involved in protein-protein interaction; a FYRN and a FRYC domain; a transactivation domain which binds CBP; may acetylates H3 and H4 in the HOX area; a SET domain: methyltransferase; methyltates H3, including histones in the H ...
Alternatively Spliced Genes
... conserved among different species of metazoans. In Saccharomyces cerevisiae, although only a small percentage of genes undergo splicing, more than 100 genes have been identified that are either dedicated to or involved in pre-mRNA splicing. In mammals, pre-mRNA splicing is a crucial step for gene exp ...
... conserved among different species of metazoans. In Saccharomyces cerevisiae, although only a small percentage of genes undergo splicing, more than 100 genes have been identified that are either dedicated to or involved in pre-mRNA splicing. In mammals, pre-mRNA splicing is a crucial step for gene exp ...
Neurospora tetrasperma crosses heterozygous for hybrid
... can either be of opposite mating types (i.e. [mat A + mat a]) or of the same mating type (figure 2). Het1-type progeny with nuclei of the same mating type can be generated by a crossover proximal to a translocation breakpoint, such that the mat locus undergoes first-division segregation, whereas the ...
... can either be of opposite mating types (i.e. [mat A + mat a]) or of the same mating type (figure 2). Het1-type progeny with nuclei of the same mating type can be generated by a crossover proximal to a translocation breakpoint, such that the mat locus undergoes first-division segregation, whereas the ...
Methods Five 1536-SNP GoldenGate assays (Fig. 1) Three pilot
... applied to POPA1 and POPA2 (NR, TJC, SC). Since heterozygotes were very rare, this means that with only a few exceptions BOPA1 SNPs had a minor allele frequency of at least 0.08. At the time when BOPA1 was designed, BOPA1 included 1314 mapped and 222 unmapped SNPs. To the extent of results presented ...
... applied to POPA1 and POPA2 (NR, TJC, SC). Since heterozygotes were very rare, this means that with only a few exceptions BOPA1 SNPs had a minor allele frequency of at least 0.08. At the time when BOPA1 was designed, BOPA1 included 1314 mapped and 222 unmapped SNPs. To the extent of results presented ...
The Parasexual Cycle in Candida albicans Provides an
... overall cell ploidy (unpublished data). PCR of the MTL loci was also used to detect possible jackpot effects, where several gal1 progeny might have been derived from a single cell having undergone a chromosome loss event. Where possible, progeny cells with different combinations of MTL alleles were ...
... overall cell ploidy (unpublished data). PCR of the MTL loci was also used to detect possible jackpot effects, where several gal1 progeny might have been derived from a single cell having undergone a chromosome loss event. Where possible, progeny cells with different combinations of MTL alleles were ...
Forche et al. 2008 PLoS Biology
... overall cell ploidy (unpublished data). PCR of the MTL loci was also used to detect possible jackpot effects, where several gal1 progeny might have been derived from a single cell having undergone a chromosome loss event. Where possible, progeny cells with different combinations of MTL alleles were ...
... overall cell ploidy (unpublished data). PCR of the MTL loci was also used to detect possible jackpot effects, where several gal1 progeny might have been derived from a single cell having undergone a chromosome loss event. Where possible, progeny cells with different combinations of MTL alleles were ...
jing is required for wing development and to establish the
... screened an imaginal disc cDNA library constructed in λgt10 using a fragment of genomic DNA adjacent to the P element insertion site as probe. We identified four independent partial clones that were grouped into two non-overlapping families. Our cDNAs, together with additional cDNAs identified by BD ...
... screened an imaginal disc cDNA library constructed in λgt10 using a fragment of genomic DNA adjacent to the P element insertion site as probe. We identified four independent partial clones that were grouped into two non-overlapping families. Our cDNAs, together with additional cDNAs identified by BD ...
Modified uridine at wobble position in tRNA of
... (ncm5Um), 5-methoxycarbonylmethyluridine (mcm5U), and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U). Based on the complex structure of these nucleosides, it is likely that their formation requires several synthesis steps. The Elongator complex consisting of proteins Elp1p - Elp6p, and the proteins ...
... (ncm5Um), 5-methoxycarbonylmethyluridine (mcm5U), and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U). Based on the complex structure of these nucleosides, it is likely that their formation requires several synthesis steps. The Elongator complex consisting of proteins Elp1p - Elp6p, and the proteins ...
Characterization of the Phosphoenolpyruvate Carboxykinase Gene
... in gluconeogenesis. To investigate the role of PEP carboxykinase for growth and amino acid production, the respective pck gene was isolated, characterized and used for construction and analysis of mutants and overexpressing strains. Sequence analysis of the pck gene predicts a polypeptide of 610 ami ...
... in gluconeogenesis. To investigate the role of PEP carboxykinase for growth and amino acid production, the respective pck gene was isolated, characterized and used for construction and analysis of mutants and overexpressing strains. Sequence analysis of the pck gene predicts a polypeptide of 610 ami ...
Mapping Polygenes - University of Warwick
... macromutant alleles are rare in natural populations. Without allelic variation there is no segregation, and without segregation no linkage tests can be performed to detect polygenes. The level of polymorphism maintained at any given locus in natural populations is determined by many factors, includi ...
... macromutant alleles are rare in natural populations. Without allelic variation there is no segregation, and without segregation no linkage tests can be performed to detect polygenes. The level of polymorphism maintained at any given locus in natural populations is determined by many factors, includi ...
Thesis-1965R-K29r
... nucleoli irradiated in the latter part of midprnphase and all those irradiated in late and very late prophase divide. ...
... nucleoli irradiated in the latter part of midprnphase and all those irradiated in late and very late prophase divide. ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.