A versatile toolbox for PCR-based tagging of yeast genes: new
... Copyright 2004 John Wiley & Sons, Ltd. ...
... Copyright 2004 John Wiley & Sons, Ltd. ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... potential degenerate primers. The H-Contraction algorithm starts with a fully degenerate primer. The algorithm then examines all degenerate positions and chooses a position i, with character b, whose count D(b, i) is the smallest and discards b from position i in the primer. It then repeats this pro ...
... potential degenerate primers. The H-Contraction algorithm starts with a fully degenerate primer. The algorithm then examines all degenerate positions and chooses a position i, with character b, whose count D(b, i) is the smallest and discards b from position i in the primer. It then repeats this pro ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
Early days of tRNA research: Discovery, function, purification and
... molecules. The groups of Berg (figure 4), Lipmann, Novelli, Schweet and Zamecnik were among the early contributors (Berg 1956, 2003; Berg et al 1961; Davie et al 1956; DeMoss and Novelli 1956; Schweet et al 1958; Zamecnik et al 1958). It was quickly found that each aminoacyltRNA synthetase is specific ...
... molecules. The groups of Berg (figure 4), Lipmann, Novelli, Schweet and Zamecnik were among the early contributors (Berg 1956, 2003; Berg et al 1961; Davie et al 1956; DeMoss and Novelli 1956; Schweet et al 1958; Zamecnik et al 1958). It was quickly found that each aminoacyltRNA synthetase is specific ...
Cloning and analysis of the mobile element gypsy from D. virilis
... The D. virilis genomic library was screened with the probes derived from regions corresponding to the first and the third ORFs and LTR of gypsy from D. melanogaster. Two clones containing presumably full-length copies were picked for subcloning and sequencing. The nucleotide sequence of one of the c ...
... The D. virilis genomic library was screened with the probes derived from regions corresponding to the first and the third ORFs and LTR of gypsy from D. melanogaster. Two clones containing presumably full-length copies were picked for subcloning and sequencing. The nucleotide sequence of one of the c ...
Cloning and sequencing of a gene encoding acidophilic amylase
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
... Agnano 101 (Buonocore et at., 1976) and A-2 (Kanno, 1986), and from Bacillus sp. 11-1s (Uchino, 1982). The pH optima of the three enzymes ranged from 2-0 to 3.5, temperature optima between 70 and 75 "C and apparent molecular masses from 54 to 68 kDa. The molecular mass of the B. acidocaldarius ATCC ...
Molecular Diagnostics in Clinical Microbiology
... preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective solutions to avoid these issues have been introduced. Complicated extraction protocols using undesired chemicals are replaced with commercial filter col ...
... preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective solutions to avoid these issues have been introduced. Complicated extraction protocols using undesired chemicals are replaced with commercial filter col ...
Mendelian Genetics
... 1 Phenotypic traits are controlled by pairs of genes which act as individual units of inheritance 2 In genes that have multiple alleles (variations) the presence of some traits, called dominant traits, mask the presence of recessive traits 3 Gene pairs segregate randomly during gamete formation with ...
... 1 Phenotypic traits are controlled by pairs of genes which act as individual units of inheritance 2 In genes that have multiple alleles (variations) the presence of some traits, called dominant traits, mask the presence of recessive traits 3 Gene pairs segregate randomly during gamete formation with ...
Nonribosomal peptide synthesis in Aspergillus
... nidulans and Aspergillus oryzae have also been sequenced (Galagan et al., 2005; Machida et al., 2005). Although extensive in silico annotation of the A. fumigatus genome is in place, biochemical support for most gene identification is limited and a significant number of genes (approx. 5 % of total) ...
... nidulans and Aspergillus oryzae have also been sequenced (Galagan et al., 2005; Machida et al., 2005). Although extensive in silico annotation of the A. fumigatus genome is in place, biochemical support for most gene identification is limited and a significant number of genes (approx. 5 % of total) ...
Visualizations of Microarray Data in Partek Genomics Suite 6.6
... PGS’s function Sort rows by prototype enables you to identify genes with similar expression profiles. For instance, if a gene with an interesting expression pattern has been detected, using Sort by prototype makes it possible to find other genes that have a similar pattern of intensity values. Altho ...
... PGS’s function Sort rows by prototype enables you to identify genes with similar expression profiles. For instance, if a gene with an interesting expression pattern has been detected, using Sort by prototype makes it possible to find other genes that have a similar pattern of intensity values. Altho ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
DOC-file of additional text
... Putative orthologous as well as paralogous proteins were identified for 56 completely sequenced proteobacteria species (Supplementary figure 1) using STRING 3, a database that integrates and extends the widely used clusters of orthologous groups (COG)4. To exclude paralogues, only genes present as a ...
... Putative orthologous as well as paralogous proteins were identified for 56 completely sequenced proteobacteria species (Supplementary figure 1) using STRING 3, a database that integrates and extends the widely used clusters of orthologous groups (COG)4. To exclude paralogues, only genes present as a ...
Pitx1 and Pitx2 are required for development of hindlimb buds
... proximodistal (PD) domains in the limb appears to be defined very early in the growing limb bud. In particular, the proximal limb domain where stylopod (femur or humerus) will form is marked by expression of other homeobox-containing transcription factors, Meis1 and Meis2, and gain-of-function exper ...
... proximodistal (PD) domains in the limb appears to be defined very early in the growing limb bud. In particular, the proximal limb domain where stylopod (femur or humerus) will form is marked by expression of other homeobox-containing transcription factors, Meis1 and Meis2, and gain-of-function exper ...
DNA
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
Primer on Molecular Biology
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
Introductory genetics for veterinary students
... and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of imprinted QTL in animal genetics ...
... and q alleles will be alternatively “fixed” in the alternate F0 lines? Hence, that the QTL genotype of all F1 animals is the same? Especially when this is not the case for the SNP or microsatellite markers. This has lead to many erroneous conclusions of imprinted QTL in animal genetics ...
Distribution and structural variation of the she pathogenicity island in
... coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), Yersinia enterocolitica and Salmonella enterica serovar Typhimurium. The study showed that the she PAI has undergone a variety of structural changes, de®ned by the presence or absence of speci®c marker genes in the PAI. The ...
... coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), Yersinia enterocolitica and Salmonella enterica serovar Typhimurium. The study showed that the she PAI has undergone a variety of structural changes, de®ned by the presence or absence of speci®c marker genes in the PAI. The ...
Lactococcus lactis LM0230 contains a single
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
The Complex Inheritance of Maize Domestication Traits and Gene
... I want to extend my thanks to John Doebley for making this dissertation possible. John has been a constant voice of encouragement and insight throughout my graduate career. He has been instrumental in keeping me focused on the big question, while allowing me the freedom to chase down side interests ...
... I want to extend my thanks to John Doebley for making this dissertation possible. John has been a constant voice of encouragement and insight throughout my graduate career. He has been instrumental in keeping me focused on the big question, while allowing me the freedom to chase down side interests ...
Mending Mendelism - SHiPS Resource Center
... implies, awkwardly, that the healthy condition (recessive) is only the ‘‘absence’’ of the disease. As these cases demonstrate, not all recessive traits are nonfunctional. A recessive phenotype is an alternative phenotype. Dominant and recessive traits do not map simply onto a trait’s presence or ab ...
... implies, awkwardly, that the healthy condition (recessive) is only the ‘‘absence’’ of the disease. As these cases demonstrate, not all recessive traits are nonfunctional. A recessive phenotype is an alternative phenotype. Dominant and recessive traits do not map simply onto a trait’s presence or ab ...
Activation of the Interleukin-3 Gene by Chromosome
... IL-3 gene. The two chromosome 5 breakpoints were separated by less than 500 bp. The genomic structure in Cases 1 and 2 suggested that a normal IL-3 gene product was over-expressed as a result of the altered promotor structure. This would predict that the IL-3 gene on the translocated chromosome was ...
... IL-3 gene. The two chromosome 5 breakpoints were separated by less than 500 bp. The genomic structure in Cases 1 and 2 suggested that a normal IL-3 gene product was over-expressed as a result of the altered promotor structure. This would predict that the IL-3 gene on the translocated chromosome was ...
Genomics of adaptation and speciation in cichlid fishes: recent
... (Danio rerio) have shown its indispensable role in epithelial morphogenesis and skin development [18], making this a candidate gene for future analyses of body shape and trophic differentiation associated with adaptation in haplochromine cichlids. Ecologically divergent cichlid species can differ dr ...
... (Danio rerio) have shown its indispensable role in epithelial morphogenesis and skin development [18], making this a candidate gene for future analyses of body shape and trophic differentiation associated with adaptation in haplochromine cichlids. Ecologically divergent cichlid species can differ dr ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.