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... problems nor with small non-constitutive membrane-active peptides because binding usually induces secondary structure (partitioning-folding coupling). Thus, as is often the case in solution thermodynamics, the reference state must be a virtual one. This is defined it by means of an experimental inte ...
Sander van Riet 13 June Reviewer Gene co
Sander van Riet 13 June Reviewer Gene co

... Gene co-expression is widely used to find new associations between different genes. The paper of IvIiev et al. uses this concept together with Protein Atlas project in order to identify new genes associated with motile cilia. Data from 3 different regions known to harbour motile cilia obtained from ...
here
here

... In the α,β-hydrolase family, a triad of amino acids (histodine, aspartate and serine) are conserved. They occur in the active site in close proximity to one another, where they interact to allow Serine to act as a nucleophile. This triad is present in MhpC, but it is unknown whether it has the same ...
Lab 7: Emission Spectra
Lab 7: Emission Spectra

... This is a hydrogen absorption spectrum. It looks like a continuous spectrum with a few specific colors missing, and that's exactly what it is. Those missing colors are light with a specific wavelength that has been absorbed by a hydrogen atom. What causes absorption lines?  The electrons in atoms h ...
ch16_LecturePPT
ch16_LecturePPT

structure_property
structure_property

... Almost of all alpha helix is right handed. One residue to the next is about 100 degree 3D translation of 1.5 Angstrom in proteins. 3-10 helix: ith residue’s NH  O=C of i+3th residue. Pi-helix  ith => i+5th residue. Where the stability is from  ...
Introduction to Proteins: Biotech 2
Introduction to Proteins: Biotech 2

... cheap, proteins not expressed properly Mammalian Cell Systems – finicky, grow slowly, and expensive, BUT processes human proteins correctly ...
Physics - Kendriya Vidyalaya, Gill Nagar
Physics - Kendriya Vidyalaya, Gill Nagar

Introduction to Macromolecular Structures
Introduction to Macromolecular Structures

... all over the whole molecule. If the fitting suddenly becomes bad in some region, it may indicate that something wrong with the fitting. Missing density is much better than extra density. It’s rarely seen that there is a blob of extra density for Gly, Ala or Pro residue. The model should make chemica ...
LEP 2.1.01 Measuring the velocity of light
LEP 2.1.01 Measuring the velocity of light

Structure determination by X
Structure determination by X

Problem 2
Problem 2

What_Is_Light
What_Is_Light

... Examples of other fundamental constants are charge of an electron, mass of an electron, Planck’s constant, etc. Light can only be studied indirectly, that is, in terms of how it behaves. ...
Dipole Moment & Polarity
Dipole Moment & Polarity

Kay Hofmann - Tresch Group
Kay Hofmann - Tresch Group

... too much, the correcpt peptide can be identified by a good correlation of expected and observed pattern. Problems with polymorphisms, modifications, other unexpected things. ...
force on moving charge
force on moving charge

... between two “particles”, a photon, and an electron. The way to do this is to use two important conservation laws: Conservation of energy, and of momentum. No energy or momentum is added to the system of two particles from the outside, so both should remain constant during the collision. Initially th ...
Amino Acids 2
Amino Acids 2

... A disulfide bond is a covalent linkage formed by the sulfhydryl group (-SH) of two cysteine residues to form cystine • The folding of the polypeptide chain brings the cysteine residues near each other • Disulfide linkage contributes to the stability of the three-dimensional shape of the protein mole ...
DNA sequence of Exenatide to be prepared using Phosphoramidite
DNA sequence of Exenatide to be prepared using Phosphoramidite

Nature`s origami: protein folding mistakes and diseases
Nature`s origami: protein folding mistakes and diseases

... What is a receptor? A receptor may be thought of as a lock that allows an outside ligand or key to activate a response inside the cell. Located on the cell membrane or surface, a receptor is made up of a string of precisely folded amino acids. A team of Center scientists has discovered that the caus ...
Prov Exam Q`ns B2 B3 B4
Prov Exam Q`ns B2 B3 B4

Polyacrylamide gels
Polyacrylamide gels

... under the influence of an applied electric field •PAGE used for proteins and small pieces of DNA •Similar idea to separation of DNA in agarose •2 major determinants of particle mobility are charge/mass ratio and structure •Polyacrylamide has smaller pore size- better resolution (2bp differences in D ...
FTIR Talk Letter Vol. 18 - Shimadzu Scientific Instruments
FTIR Talk Letter Vol. 18 - Shimadzu Scientific Instruments

SURVEY OF BIOCHEMISTRY - Georgia Institute of Technology
SURVEY OF BIOCHEMISTRY - Georgia Institute of Technology

... Ile, Ala ...
RISE-Workshop
RISE-Workshop

Waves, incl. Electromagnetic Waves, Light
Waves, incl. Electromagnetic Waves, Light

... identical amplitude and wavelength, traveling in the same medium (therefore with the same speed!) in opposite directions. One wave is ½ a wavelength displaced relative to the other. Their interference pattern is (a) a succession of only crests and no troughs. (b) a flat line, i.e. perfectly destruct ...
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Circular dichroism



Circular dichroism (CD) is dichroism involving circularly polarized light, i.e., the differential absorption of left- and right-handed light. Left-hand circular (LHC) and right-hand circular (RHC) polarized light represent two possible spin angular momentum states for a photon, and so circular dichroism is also referred to as dichroism for spin angular momentum. This phenomenon was discovered by Jean-Baptiste Biot, Augustin Fresnel, and Aimé Cotton in the first half of the 19th century. It is exhibited in the absorption bands of optically active chiral molecules. CD spectroscopy has a wide range of applications in many different fields. Most notably, UV CD is used to investigate the secondary structure of proteins. UV/Vis CD is used to investigate charge-transfer transitions. Near-infrared CD is used to investigate geometric and electronic structure by probing metal d→d transitions. Vibrational circular dichroism, which uses light from the infrared energy region, is used for structural studies of small organic molecules, and most recently proteins and DNA.
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