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Correlated diffraction and fluorescence in the backscattering
Correlated diffraction and fluorescence in the backscattering

... found close to 8° for the Troides magellanus yellow scales. The integer m should be chosen so that the resulting value of sin共␾ − ␣兲 is smaller than unity. The special value m = 0 describes the specular reflection, which does not produce any color separation in the emerging light, since 共in the scal ...
Optical System design
Optical System design

... optimisation method, but with several enhancements [4], such as an extended line search option. The merit function is powerful and flexible. Wizards exist to setup the function, but a whole range of defects can be added by drag and drop. In addition, there is a number of aids [4] such as variable se ...
Resolution Power And Intensity Distribution Using Synthetic Square
Resolution Power And Intensity Distribution Using Synthetic Square

... number of rows and columns are choosing (T=2,4,6,8,10),that mean the apertures allow light to pass through it are (2,8,18,32,50) . The two cases (G=1,G=-1) are solved , the result appear similar in two cases at ideal system or contain focus error and aberrations which are chosen in research. Figure ...
Optical Fiber and Communication Course Code: Credit
Optical Fiber and Communication Course Code: Credit

... All the topics covered in the syllabus will be correlated with its applications in research & developments of Fiber optics and communications. In order to inculcate problem solving ability in students’ time to time quiz, viva, home assignments and class tests will be conducted during the progress of ...
Holographic methods for phase microscopic objects study
Holographic methods for phase microscopic objects study

Nonconfocal Differential Interferometry Sensing Scheme for
Nonconfocal Differential Interferometry Sensing Scheme for

... Developing a force microscope to monitor the deflection of the cantilever consists of using optical interferometry,1–6) capacitance,7,8) point-contact current-imaging,9) beam deflection,10–14) and magnetoresistive head.15) Constructing the force-sensing cantilever/tip device is a major task for the fo ...
Suppression of optical damage at 532 nm in
Suppression of optical damage at 532 nm in

... initially occurs, with the signal reaching a minimum at ~3 seconds, and then gradually increasing. As shown in Fig. 3(e), the signal at t = 300 sec is higher than that recorded immediately after unblocking the beam. For comparison, we have included the time dependence of the transmitted laser beam w ...
Theory of relativistic optical harmonic generation
Theory of relativistic optical harmonic generation

... found a closed-form expression analogous to (4). For either type of polarization, the saturation of the harmonic power can be understood from the perspective that even at very high intensities the electron excursion from its equilibrium position is limited to a distance of the order of the wavelengt ...
a flat high-frequency scanning micromirror - EECS: www
a flat high-frequency scanning micromirror - EECS: www

Time-domain optical data storage by use of Raman coherent population trapping
Time-domain optical data storage by use of Raman coherent population trapping

Microscope Basics
Microscope Basics

Structure and Imaging of a Transmission Electron Microscope (TEM)
Structure and Imaging of a Transmission Electron Microscope (TEM)

Educator Resource Center in the Lyman Library
Educator Resource Center in the Lyman Library

Tutorial: Are You Safe at a Laser Light Show?
Tutorial: Are You Safe at a Laser Light Show?

A day in the life of a Laser scientist
A day in the life of a Laser scientist

Optical Measurements of Pollution Dispersion at
Optical Measurements of Pollution Dispersion at

Technical Article
Technical Article

... In the decade since its introduction, optical coherence tomography (OCT) has proven to be an important modality for micrometer-scale imaging [1]. To date, virtually all systems built for clinical and biological use have employed a variable group delay reference arm to coherently gate backscattered l ...
Enhanced 3D spatial resolution in quantitative phase
Enhanced 3D spatial resolution in quantitative phase

... samples using quadri-wave interferometry,” Proc. SPIE 8587, 85871D (2013). ...
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Figure 2. Turbo FT Laser Lineshape

... 7.2 NIR/SWIR TURBO INSTRUMENT With the use of different optics and detectors, a Turbo FT can be built to operate in the NIR/SWIR spectral region from 1-2.5 microns. In this configuration, there are much more stringent alignment and drift requirements for the interferometer, and much higher operating ...
PASSIVE MILLIMETER-WAVE IMAGING WITH EXTENDED DEPTH OF FIELD AND SPARSE DATA
PASSIVE MILLIMETER-WAVE IMAGING WITH EXTENDED DEPTH OF FIELD AND SPARSE DATA

Optimal pupil size in the human eye for axial resolution
Optimal pupil size in the human eye for axial resolution

Handbook of Optical Filters
Handbook of Optical Filters

... have a quantum efficiency of approximately 0.3, but the actual value can be reduced by processes known as quenching, one of which is photobleaching. The combination of these factors, in addition to the fact that many specimens have very small amounts of stained material in the observed field of view ...
Bi Imaging - MicroscopyU
Bi Imaging - MicroscopyU

... cited by one wavelength (this is because of the extended excitation spectra associated with multiphoton absorption), and the excitation of fluorescence is limited to a tiny volume within the sample so that any fluorescence must emanate from that optical plane only (Table 1). CO-LOCALIZATION ANALYSIS ...
Download PDF
Download PDF

Simultaneous spatial and temporal focusing: A route towards
Simultaneous spatial and temporal focusing: A route towards

... surgery or back-side processing of glass, ultrashort laser pulses are focused deeply into the material with low NA.5, 6 As a result, the extreme intensities are not only obtained at the geometrical focus, but may also be achieved already during the propagation towards the focal plane and may evoke n ...
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Super-resolution microscopy

Super-resolution microscopy is a form of light microscopy. Due to the diffraction of light, the resolution of conventional light microscopy is limited as stated by Ernst Abbe in 1873. A good approximation of the resolution attainable is the full width at half maximum (FWHM) of the point spread function, and a precise widefield microscope with high numerical aperture and visible light usually reaches a resolution of ~250 nm.Super-resolution techniques allow the capture of images with a higher resolution than the diffraction limit. They fall into two broad categories, ""true"" super-resolution techniques, which capture information contained in evanescent waves, and ""functional"" super-resolution techniques, which use clever experimental techniques and known limitations on the matter being imaged to reconstruct a super-resolution image.True subwavelength imaging techniques include those that utilize the Pendry Superlens and near field scanning optical microscopy, the 4Pi Microscope and structured illumination microscopy technologies like SIM and SMI. However, the majority of techniques of importance in biological imaging fall into the functional category.There are two major groups of methods for functional super-resolution microscopy: Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM. Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.On October 8th, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, W.E. Moerner and Stefan Hell for ""the development of super-resolved fluorescence microscopy,"" which brings ""optical microscopy into the nanodimension"".
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