Secretion of Beta-Lactamase Requires the Carboxyl End of the Protein.
... that the frame be shifted into the + 1 frame (Roth, 1974). The next termination codon was found in this frame on the nucleotide sequence (Sutcliffe. 1978) and the length so determined was used to calculate the molecular weight. The observed molecular weights were calculated from autoradiograms of SD ...
... that the frame be shifted into the + 1 frame (Roth, 1974). The next termination codon was found in this frame on the nucleotide sequence (Sutcliffe. 1978) and the length so determined was used to calculate the molecular weight. The observed molecular weights were calculated from autoradiograms of SD ...
Chlamydia pneumoniae CdsQ functions as a multi
... protein, translocated actin recruitment protein (TARP), which facilitates bacterial internalization into a plasma-membrane derived vacuole, known as an inclusion [17, 18]. Once within the inclusion, the EB undergoes a transformation, differentiating into the metabolically active form, the reticulate ...
... protein, translocated actin recruitment protein (TARP), which facilitates bacterial internalization into a plasma-membrane derived vacuole, known as an inclusion [17, 18]. Once within the inclusion, the EB undergoes a transformation, differentiating into the metabolically active form, the reticulate ...
Overview of tag protein fusions
... fusion proteins on streptavidin columns (Schmidt and Skerra 1993). Streptavidin mutants with a specific mutation at position 44, 45, and 47 have a higher affinity for the octapeptide Strep-tag II than for the native form (for the sequence, see Table 2; Schmidt et al. 1996; Voss and Skerra 1997; Korn ...
... fusion proteins on streptavidin columns (Schmidt and Skerra 1993). Streptavidin mutants with a specific mutation at position 44, 45, and 47 have a higher affinity for the octapeptide Strep-tag II than for the native form (for the sequence, see Table 2; Schmidt et al. 1996; Voss and Skerra 1997; Korn ...
Four-body Statistical Potentials
... Four-Body Potentials Scoring Livebench 6 and CASP5 predictions Livebench Automated evaluation of structure prediction servers Set 6 had 32 “easy” and 66 “hard” targets CASP 5 3D coordinate models submitted for 56 targets Native structure of 33 targets has been released - rank 3D predictions using f ...
... Four-Body Potentials Scoring Livebench 6 and CASP5 predictions Livebench Automated evaluation of structure prediction servers Set 6 had 32 “easy” and 66 “hard” targets CASP 5 3D coordinate models submitted for 56 targets Native structure of 33 targets has been released - rank 3D predictions using f ...
dependent phosphotransferase system – two highly similar glucose
... suggests additional regulation by carbon catabolite repression. As described previously by the authors, both genes can be expressed in Escherichia coli under control of their own promoters. Two putative promoters are located upstream of glcA, and both were found to initiate transcription in E. coli. ...
... suggests additional regulation by carbon catabolite repression. As described previously by the authors, both genes can be expressed in Escherichia coli under control of their own promoters. Two putative promoters are located upstream of glcA, and both were found to initiate transcription in E. coli. ...
2nd Nutritional Timing Window and BCAA / FFAA
... Free Form Amino Acids are singular molecules, not attached by ...
... Free Form Amino Acids are singular molecules, not attached by ...
Full Text - Molecular Biology and Evolution
... effect decreases as cellular or organismal complexity increases. Water-soluble proteins have on average 2.7 times more orthologs than membrane proteins in prokaryotes. The factor decreases to 2.4 in unicellular eukaryotes, and to 1.7 in multicellular eukaryotes (fig. 3A; one-way analysis of variance ...
... effect decreases as cellular or organismal complexity increases. Water-soluble proteins have on average 2.7 times more orthologs than membrane proteins in prokaryotes. The factor decreases to 2.4 in unicellular eukaryotes, and to 1.7 in multicellular eukaryotes (fig. 3A; one-way analysis of variance ...
ERdj5, an Endoplasmic Reticulum (ER)
... human expressed sequence tag (EST) clones encoding proteins similar to thioredoxin. Some of these EST entries were found to encode a putative protein containing both a DnaJ domain and four putative thioredoxin-like active sites. Based on these sequences, the following primers were designed and used ...
... human expressed sequence tag (EST) clones encoding proteins similar to thioredoxin. Some of these EST entries were found to encode a putative protein containing both a DnaJ domain and four putative thioredoxin-like active sites. Based on these sequences, the following primers were designed and used ...
Introduction to Proteins II
... The Sequence of Amino Acids in a Protein [S23] a. The exact sequence of amino acids is unique for each protein. Protein Structure [S24] a. Is all the information necessary for a protein to fold just based on that primary sequence? The native structure in how a protein folds – is it based completely ...
... The Sequence of Amino Acids in a Protein [S23] a. The exact sequence of amino acids is unique for each protein. Protein Structure [S24] a. Is all the information necessary for a protein to fold just based on that primary sequence? The native structure in how a protein folds – is it based completely ...
Application 1
... nanoparticles can serve as useful detection and targeting agents. These AuNPs when encapsulated with carbohydrate ligands can serve as a useful affinity probe for rapid, efficient separation of target proteins followed by on-probe analysis using mass spectroscopy. This technique of protein targeting ...
... nanoparticles can serve as useful detection and targeting agents. These AuNPs when encapsulated with carbohydrate ligands can serve as a useful affinity probe for rapid, efficient separation of target proteins followed by on-probe analysis using mass spectroscopy. This technique of protein targeting ...
Glycosylation of Proteins - Structure, Function and Analysis
... Golgi apparatus of each cell. This variety of profiles gives cells the flexibility to produce glycoproteins with specific properties related to the function of that cell but from a limited number of monosaccharides. Glycans have been shown to have a range of specific biological roles: by targeting o ...
... Golgi apparatus of each cell. This variety of profiles gives cells the flexibility to produce glycoproteins with specific properties related to the function of that cell but from a limited number of monosaccharides. Glycans have been shown to have a range of specific biological roles: by targeting o ...
sv-lncs - School of Mathematical and Computer Sciences
... clustering, measured by clustering coefficient. The clustering coefficient measures the local cohesiveness around a node, and it is defined, for any node i, as the fraction of neighbours of i that are connected to each other. [8] As pointed in [9], each module may be reduced to a set of triangles, a ...
... clustering, measured by clustering coefficient. The clustering coefficient measures the local cohesiveness around a node, and it is defined, for any node i, as the fraction of neighbours of i that are connected to each other. [8] As pointed in [9], each module may be reduced to a set of triangles, a ...
Chemical Disinfection
... u Used along with chlorine to disinfect water. u Helps neutralize unpleasant tastes and odors. u More effective killing agent than chlorine, but less stable and more expensive. u Highly reactive form of oxygen. u Made by exposing oxygen to electricity or UV light. ...
... u Used along with chlorine to disinfect water. u Helps neutralize unpleasant tastes and odors. u More effective killing agent than chlorine, but less stable and more expensive. u Highly reactive form of oxygen. u Made by exposing oxygen to electricity or UV light. ...
Plant nuclear bodies
... domains represent dynamic regions of the nucleus where signalling molecules and effector molecules (such as transcription factors or inhibitors) can associate into complexes that determine transcriptional and cellular activity. The differential formation of phytochromecontaining domains that contain ...
... domains represent dynamic regions of the nucleus where signalling molecules and effector molecules (such as transcription factors or inhibitors) can associate into complexes that determine transcriptional and cellular activity. The differential formation of phytochromecontaining domains that contain ...
Anxiety Study Abstract
... in those suffering from Social Phobia were employed to measure changes in anxiety in response to a stimulus as part of a double blind placebo controlled, cross-over study with a wash-out period of one week between study sessions. Subjects were randomly assigned to start with either: (1) protein sour ...
... in those suffering from Social Phobia were employed to measure changes in anxiety in response to a stimulus as part of a double blind placebo controlled, cross-over study with a wash-out period of one week between study sessions. Subjects were randomly assigned to start with either: (1) protein sour ...
Supplemental Figures and Tables
... (fused to GAL4 DNA binding domain) and prey (fused to GAL4 activation domain). (B) GUS expression in leaf abaxial (lower) epidermal cells of tobacco transiently transformed with pZPR3-uidA and 35S-REV* or pZPR3-uidA and 35S-REV*-ΔLZ constructs. microRNA resistant REV is designated REV*. REV homo-dim ...
... (fused to GAL4 DNA binding domain) and prey (fused to GAL4 activation domain). (B) GUS expression in leaf abaxial (lower) epidermal cells of tobacco transiently transformed with pZPR3-uidA and 35S-REV* or pZPR3-uidA and 35S-REV*-ΔLZ constructs. microRNA resistant REV is designated REV*. REV homo-dim ...
Recombinant human insulin-11. Size-exclusion HPLC of
... molecules may lead to partial adsorption, especially in case of high molecular mass or the formation of complexes of water-soluble biopolymers, for which adsorption may by sufficiently high due to a great number of contacts, in addition to secondary effects. In order to reduce the contribution of hy ...
... molecules may lead to partial adsorption, especially in case of high molecular mass or the formation of complexes of water-soluble biopolymers, for which adsorption may by sufficiently high due to a great number of contacts, in addition to secondary effects. In order to reduce the contribution of hy ...
Amino acid theory sheet
... Most of amino acids have four different groups attached to the central carbon atom, (apart from glycine) this means that they have chiral centres. This causes each acid to have two isomers whose mirror images cannot be superimposed onto one another, which is called stereoisomerism. The optical isom ...
... Most of amino acids have four different groups attached to the central carbon atom, (apart from glycine) this means that they have chiral centres. This causes each acid to have two isomers whose mirror images cannot be superimposed onto one another, which is called stereoisomerism. The optical isom ...
Comparative Modeling of Mainly
... NCBI’s protein sequence database: • Many proteins of similar function to the known betahelices; some with similar sequences. • A significant fraction are characterized as microbial ...
... NCBI’s protein sequence database: • Many proteins of similar function to the known betahelices; some with similar sequences. • A significant fraction are characterized as microbial ...
Getting RNA and Protein in Phase
... over, although filaments are clearly visible in pure LCS hydrogels, light microscopic images of the b-isox precipitate from cell lysates show an amorphous aggregate (no high-resolution EM data are shown). Finally, b-isox precipitates solubilize upon warming to 37" C, suggesting that the cross-b inte ...
... over, although filaments are clearly visible in pure LCS hydrogels, light microscopic images of the b-isox precipitate from cell lysates show an amorphous aggregate (no high-resolution EM data are shown). Finally, b-isox precipitates solubilize upon warming to 37" C, suggesting that the cross-b inte ...
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[PDF]
... over, although filaments are clearly visible in pure LCS hydrogels, light microscopic images of the b-isox precipitate from cell lysates show an amorphous aggregate (no high-resolution EM data are shown). Finally, b-isox precipitates solubilize upon warming to 37" C, suggesting that the cross-b inte ...
... over, although filaments are clearly visible in pure LCS hydrogels, light microscopic images of the b-isox precipitate from cell lysates show an amorphous aggregate (no high-resolution EM data are shown). Finally, b-isox precipitates solubilize upon warming to 37" C, suggesting that the cross-b inte ...
Description of the KDD-Cup 2004 Protein Data
... Description of the KDD-Cup 2004 Protein Data Author: Ron Elber, Department of Computer Science, Cornell University The data are generated by the program LOOPP (Learning Observing and Outputting Protein Patterns) that is a product of the group of Ron Elber, Computer Science, Cornell University. The L ...
... Description of the KDD-Cup 2004 Protein Data Author: Ron Elber, Department of Computer Science, Cornell University The data are generated by the program LOOPP (Learning Observing and Outputting Protein Patterns) that is a product of the group of Ron Elber, Computer Science, Cornell University. The L ...
Document
... multiplication of original matrix • Results in a series of PAM matrices representing different levels of similarity. • PAM250,PAM120,PAM80 PAM60 correspond to 20, 40, 50 and 60 percent similarity, respectively. • As proteins being compared decrease in similarity, the numerical value of the PAM matri ...
... multiplication of original matrix • Results in a series of PAM matrices representing different levels of similarity. • PAM250,PAM120,PAM80 PAM60 correspond to 20, 40, 50 and 60 percent similarity, respectively. • As proteins being compared decrease in similarity, the numerical value of the PAM matri ...
Ubiquitin Found to Mark Pathogen-Containing Vacuoles
... First, they primed the cells with cytokines to kick the immune system into action. Then, they stained the cells with a red dye that was specific for the ubiquitin protein. "All of a sudden we saw these beautiful rings of ubiquitin that nicely decorated the outsides of the pathogen-containing vacuole ...
... First, they primed the cells with cytokines to kick the immune system into action. Then, they stained the cells with a red dye that was specific for the ubiquitin protein. "All of a sudden we saw these beautiful rings of ubiquitin that nicely decorated the outsides of the pathogen-containing vacuole ...
Prior Art - Cabic.com
... previously known to result in lowering blood pressure. The inventors assert they have newly produced a stable crystalline form of protein P. Protein P in crystalline form is inactive. The description gives experimental data with explanations of how to make the crystals. Common prior art meth ...
... previously known to result in lowering blood pressure. The inventors assert they have newly produced a stable crystalline form of protein P. Protein P in crystalline form is inactive. The description gives experimental data with explanations of how to make the crystals. Common prior art meth ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.