Accelerating Protein ID for Deep Proteome Profiling
... Data Processing: All IDA data was processed using ProteinPilot™ Software 5.0, both individually and all in one large search. Identification results were visualized from the full ProteinPilot Reports that were automatically created. ...
... Data Processing: All IDA data was processed using ProteinPilot™ Software 5.0, both individually and all in one large search. Identification results were visualized from the full ProteinPilot Reports that were automatically created. ...
ramha1 - University of Toronto
... Random mutagenesis is a powerful tool in protein structure-function analysis. One approach is the synthesis of cDNA sequences using doped nucleoside phosphoramidites. A Turbo PASCAL program, RAMHA, was written for modelling this method of random cDNA mutagenesis on PC-compatible computers. Editing f ...
... Random mutagenesis is a powerful tool in protein structure-function analysis. One approach is the synthesis of cDNA sequences using doped nucleoside phosphoramidites. A Turbo PASCAL program, RAMHA, was written for modelling this method of random cDNA mutagenesis on PC-compatible computers. Editing f ...
Prior Art - Cabic.com
... previously known to result in lowering blood pressure. The inventors assert they have newly produced a stable crystalline form of protein P. Protein P in crystalline form is inactive. The description gives experimental data with explanations of how to make the crystals. Common prior art meth ...
... previously known to result in lowering blood pressure. The inventors assert they have newly produced a stable crystalline form of protein P. Protein P in crystalline form is inactive. The description gives experimental data with explanations of how to make the crystals. Common prior art meth ...
The proteome of the infectious bronchitis virus Beau
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
... each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified in each preparation was 399, 289 and 273, respectively. ...
- blogs@NTU
... confirming the sensitivity of our approach, the purity of the vesicles and its ability to detect novel protein interactions. Vesicle tethering components of the SYP61 compartment A variety of proteins with a role in membrane trafficking were identified in the isolated SYP61 compartment, including YI ...
... confirming the sensitivity of our approach, the purity of the vesicles and its ability to detect novel protein interactions. Vesicle tethering components of the SYP61 compartment A variety of proteins with a role in membrane trafficking were identified in the isolated SYP61 compartment, including YI ...
Identification of Domains using Structural Data
... • Neighboring residues within radius r are connected by dashed lines. • Connections between i and i + 2 have been omitted for clarity. • Label evolution is done without inverse distance weighting. ...
... • Neighboring residues within radius r are connected by dashed lines. • Connections between i and i + 2 have been omitted for clarity. • Label evolution is done without inverse distance weighting. ...
Appendix
... following the procedure described by Burns and Zydney (2000). Typical experimental data obtained using a 1 mM Bis-Tris buffer with 10 mM NaCl at pH 7 are shown in Figure A1 for an unmodified 300 kDa UltracelTM membrane and a negatively-charged version that was charged for 24 hr. The apparent zeta po ...
... following the procedure described by Burns and Zydney (2000). Typical experimental data obtained using a 1 mM Bis-Tris buffer with 10 mM NaCl at pH 7 are shown in Figure A1 for an unmodified 300 kDa UltracelTM membrane and a negatively-charged version that was charged for 24 hr. The apparent zeta po ...
A1985ALF1000002
... Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871-2, 1972. [Roche Inst. Mol. Biol , and Chem. Res. Dept , Hoffmann-La Roche, Inc., Nutley, NJ] me that he considered the proposed mechanism invalid and asked me to hold off ...
... Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871-2, 1972. [Roche Inst. Mol. Biol , and Chem. Res. Dept , Hoffmann-La Roche, Inc., Nutley, NJ] me that he considered the proposed mechanism invalid and asked me to hold off ...
PPT
... similarity scores, without the need to have high levels of similarity over their entire length • useful when looking for domains within proteins or looking for regions of genomic DNA that contain coding exons ...
... similarity scores, without the need to have high levels of similarity over their entire length • useful when looking for domains within proteins or looking for regions of genomic DNA that contain coding exons ...
Molecular Reaction Mechanisms of Proteins
... the BR to K transition, the Schiff base proton is transferred to Asp85 in the L to M transition. Simultaneously a proton is released from a protonated H-bonded network spanned by internal water molecules to the extracellular site. This network is controlled by Arg 82, Glu204 and Glu194. Asp85 reprot ...
... the BR to K transition, the Schiff base proton is transferred to Asp85 in the L to M transition. Simultaneously a proton is released from a protonated H-bonded network spanned by internal water molecules to the extracellular site. This network is controlled by Arg 82, Glu204 and Glu194. Asp85 reprot ...
slides - NMRbox
... for molecules as large as mAbs. Multivariate methods such as PCA can be used on spectra to reveal details of High Order Structure, post-translational modification, and excipient effects. Since NMR fingerprinting can potentially be performed without the need to identify peaks, it might be possibl ...
... for molecules as large as mAbs. Multivariate methods such as PCA can be used on spectra to reveal details of High Order Structure, post-translational modification, and excipient effects. Since NMR fingerprinting can potentially be performed without the need to identify peaks, it might be possibl ...
Bordoli Discussion Questions
... 3) Why were dl1107 and dl 1101 chosen for this study (what is important about the regions that are deleted in these constructs? Do they bind other proteins? 4) Is this an in vitro or an in vivo assay? 5) What protein is being detected on the blot? Why do you only see this protein even though other p ...
... 3) Why were dl1107 and dl 1101 chosen for this study (what is important about the regions that are deleted in these constructs? Do they bind other proteins? 4) Is this an in vitro or an in vivo assay? 5) What protein is being detected on the blot? Why do you only see this protein even though other p ...
The Possible DNA-binding Nature of the Regulatory Proteins
... been extensively studied and the process has been divided into six stages on the basis of the appearance of cell sections in the electron microscope (Ryter et al., 1966). It is now known that the first two of these stages (0 and I) have, in fact, nothing to do with the developmental process and can ...
... been extensively studied and the process has been divided into six stages on the basis of the appearance of cell sections in the electron microscope (Ryter et al., 1966). It is now known that the first two of these stages (0 and I) have, in fact, nothing to do with the developmental process and can ...
Coupling Coherence Distinguishes Structure Sensitivity in Protein
... derivatives, indicates that theory describes the essential aspects of ET kinetics in these complex systems. The computations include protein conformational averaging, solvation, and averaging of couplings over multiple ligand-field states with the use of methods described previously (23). The calcul ...
... derivatives, indicates that theory describes the essential aspects of ET kinetics in these complex systems. The computations include protein conformational averaging, solvation, and averaging of couplings over multiple ligand-field states with the use of methods described previously (23). The calcul ...
Using storage organelles for the accumulation and encapsulation of
... available within a species, such as transgenic tobacco, transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in ...
... available within a species, such as transgenic tobacco, transplastomic tobacco, transient expression in tobacco using Agrobacterium tumefaciens, plant viruses or hybrids thereof, hydroponic tobacco root secretion, hairy root cultures, and tobacco suspension cells [5]. This diversity has resulted in ...
FUNCTIONAL INVESTIGATION OF AN RNA BINDING PROTEIN
... DTL was recovered through its interaction with a mammalian virus RNA. This is why to characterize mammalian homologue of DTL might support and clarify certain aspects of DTL. Since the amino acid similarities shared by DTL and its relatives were located in the C-terminal half of the homologues, a mu ...
... DTL was recovered through its interaction with a mammalian virus RNA. This is why to characterize mammalian homologue of DTL might support and clarify certain aspects of DTL. Since the amino acid similarities shared by DTL and its relatives were located in the C-terminal half of the homologues, a mu ...
Hendra Virus
... studies that Nipah virus V protein interferes with interferon signaling, so it will be used as the standard to judge Hendra virus against. • Two luciferase reporter construct were assembled and transfected into human fibrosarcoma 2fTGH cells, and 293 T cells. • Cells were either transfected with HA- ...
... studies that Nipah virus V protein interferes with interferon signaling, so it will be used as the standard to judge Hendra virus against. • Two luciferase reporter construct were assembled and transfected into human fibrosarcoma 2fTGH cells, and 293 T cells. • Cells were either transfected with HA- ...
Pharming for Farmaceuticals
... aggressive and territorial to live close together. They also like to eat each other. Though the genes for dragline silk were isolated several years ago, attempts to produce it in bacterial and mammalian cell culture have failed. When the genes were put into a goat and expressed in the mammary glands ...
... aggressive and territorial to live close together. They also like to eat each other. Though the genes for dragline silk were isolated several years ago, attempts to produce it in bacterial and mammalian cell culture have failed. When the genes were put into a goat and expressed in the mammary glands ...
STRUCTURE AND ALLERGENICITY OF WHEAT GLUTEN PROTEINS
... Evidence for activity of short peptides in coeliac disease was presented by De Ritis et al. [1988], who expressed peptide activity as a function of intestinal mucosa enterocyte cell enlargement in in vitro cultures. When the 266- residue A-gliadin was cleaved with cyanogen bromide, three peptides (C ...
... Evidence for activity of short peptides in coeliac disease was presented by De Ritis et al. [1988], who expressed peptide activity as a function of intestinal mucosa enterocyte cell enlargement in in vitro cultures. When the 266- residue A-gliadin was cleaved with cyanogen bromide, three peptides (C ...
Bioinformatics analysis of experimentally determined protein
... Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, when we computationally simulate subunit compositions identical in numbers with those identi ...
... Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2002), Ho et al. (Ho 2002), and those collected in the MIPS database (Mewes 2002). In addition, when we computationally simulate subunit compositions identical in numbers with those identi ...
Statistical Analysis Using Scaffold - Proteome Software
... score for SEQUEST results, Ion score and Identity score for Mascot results) • Plots a histogram of the discriminant scores and calculates a bimodal distribution based on standard statistics to differentiate between correct and incorrect hits • Computes the probability that the match is correct at a ...
... score for SEQUEST results, Ion score and Identity score for Mascot results) • Plots a histogram of the discriminant scores and calculates a bimodal distribution based on standard statistics to differentiate between correct and incorrect hits • Computes the probability that the match is correct at a ...
What is Bioinformatics? is the application of computational techniques
... Question: How many bases of an RNA sequence are needed to encode one amino acid of a protein? (Hint: RNA’s alphabet is of size 4 and protein’s alphabet is of size 20) Bioinformatics Questions to be Answered: How do we figure out which parts of the DNA control the various chemical processes of life? ...
... Question: How many bases of an RNA sequence are needed to encode one amino acid of a protein? (Hint: RNA’s alphabet is of size 4 and protein’s alphabet is of size 20) Bioinformatics Questions to be Answered: How do we figure out which parts of the DNA control the various chemical processes of life? ...
吴冬茵
... ABSTRACT Motivation Remote homology detection: A central problem in computational biology, the classification of proteins into functional and structural classes given their amino acid sequences Discriminative method such as SVM is one of the most effective methods Explicit feature are usually ...
... ABSTRACT Motivation Remote homology detection: A central problem in computational biology, the classification of proteins into functional and structural classes given their amino acid sequences Discriminative method such as SVM is one of the most effective methods Explicit feature are usually ...
TALKING POINT The origin of the eukaryotic cell
... a limited number of gene sequences. However, as sequence data on more proteins have become available, this model has not always been supported, and sequence features and phylogenies based on several proteins give a very different picture of the history of these three groups of organisms 2°-27. Prote ...
... a limited number of gene sequences. However, as sequence data on more proteins have become available, this model has not always been supported, and sequence features and phylogenies based on several proteins give a very different picture of the history of these three groups of organisms 2°-27. Prote ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.