Scientists clarify structural basis for biosynthesis of mysterious 21st
... team analyzed the D-arm’s role in the interaction of tRNASec with O-phosphoseryl-tRNA kinase (PSTK), a protein whose selective phosphorylation is essential for selenocysteine encoding. ...
... team analyzed the D-arm’s role in the interaction of tRNASec with O-phosphoseryl-tRNA kinase (PSTK), a protein whose selective phosphorylation is essential for selenocysteine encoding. ...
translation
... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist “adapter molecules” which can read the RNA sequence (codons) and bring with it the correct amino acids a) This is the function of tRNA molecu ...
... A. Amino acids do not have direct affinity for nucleic acids 1. Therefore, mRNA can not directly serve as a template for protein synthesis 2. There must exist “adapter molecules” which can read the RNA sequence (codons) and bring with it the correct amino acids a) This is the function of tRNA molecu ...
association of drg1 and drg2 with ribosomes from pea, arabidopsis
... DRG1 antibodies recognized a single band with an apparent molecular mass of ;43 kDa protein (the deduced mass is 41.1 kDa); smaller bands (possible degradation products) were seen only occasionally. DRG2 antibodies recognized bands with apparent molecular masses of 30, 43, and 45 kDa, which varied i ...
... DRG1 antibodies recognized a single band with an apparent molecular mass of ;43 kDa protein (the deduced mass is 41.1 kDa); smaller bands (possible degradation products) were seen only occasionally. DRG2 antibodies recognized bands with apparent molecular masses of 30, 43, and 45 kDa, which varied i ...
Chapter 23: Meat Emulsions - FEA
... An emulsifier is a surface-active molecule that adsorbs to the surface of globules recently formed during homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facili ...
... An emulsifier is a surface-active molecule that adsorbs to the surface of globules recently formed during homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facili ...
Parallel Geometric Hashing Algorithm for Protein Tertiary Structure
... extended to the recognition of arbitrary rigid 3-D objects from single 2D images (Lamdan, Y., Wolfson, H.J, 1988) ...
... extended to the recognition of arbitrary rigid 3-D objects from single 2D images (Lamdan, Y., Wolfson, H.J, 1988) ...
Document
... ligand binding [27, 28], and the dynamics of the protein hydration shell [29, 30], to list just a few examples. Recent reviews can be found in Refs. [31–34] ...
... ligand binding [27, 28], and the dynamics of the protein hydration shell [29, 30], to list just a few examples. Recent reviews can be found in Refs. [31–34] ...
Heme Redox State Triggers Conformational Changes in the Ec DOS
... domains, an N-terminal sensor domain and a C-terminal PDE catalytic domain. PDE activity is dependent on the redox state of Ec DOS. The enzyme is active only when the heme is in the reduced state. The crystal structures of both the oxidized and reduced forms of the Ec DOS sensor domain (Ec DOS PAS) ...
... domains, an N-terminal sensor domain and a C-terminal PDE catalytic domain. PDE activity is dependent on the redox state of Ec DOS. The enzyme is active only when the heme is in the reduced state. The crystal structures of both the oxidized and reduced forms of the Ec DOS sensor domain (Ec DOS PAS) ...
IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT)
... Out of all the preservation techniques, fermentation is the only type that does not destroy some nutrients, can create more, and enhance others. Omega 3 fatty acids, detoxifying agents and many B vitamins including folic acid, riboflavin, niacin, thiamin, and biotin are preserved through fermentatio ...
... Out of all the preservation techniques, fermentation is the only type that does not destroy some nutrients, can create more, and enhance others. Omega 3 fatty acids, detoxifying agents and many B vitamins including folic acid, riboflavin, niacin, thiamin, and biotin are preserved through fermentatio ...
Protein Motif Recognition I Introduction
... the structure or fold of a protein provides the key to understanding its biological function, and proteins play a variety of important roles in the body (e.g., as enzymes, antibodies, etc.). Proteins may also be associated with particular human diseases, and thus, understanding protein structure may ...
... the structure or fold of a protein provides the key to understanding its biological function, and proteins play a variety of important roles in the body (e.g., as enzymes, antibodies, etc.). Proteins may also be associated with particular human diseases, and thus, understanding protein structure may ...
Leukaemia Section t(6;9)(p23;q34) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... The translocation t(6;9)(p23;q34) results in the formation of a chimeric fusion gene: DEK (6q23) and CAN (9q34). CAN is a putative oncogene which may be activated by fusion of its 3' end to other genes than DEK. One such recently reported gene is called SET and leads to expression of a SET/CAN fusio ...
... The translocation t(6;9)(p23;q34) results in the formation of a chimeric fusion gene: DEK (6q23) and CAN (9q34). CAN is a putative oncogene which may be activated by fusion of its 3' end to other genes than DEK. One such recently reported gene is called SET and leads to expression of a SET/CAN fusio ...
- Wiley Online Library
... is an RNA-binding protein that binds preferentially to uridine and guanosine-rich RNAs (Ludevid et al., 1992). Regulation studies indicated that MA16 mRNA had a basal level of expression in several tissues including embryos and seedlings. We also showed that the level of mRNA in RNA gel blots increa ...
... is an RNA-binding protein that binds preferentially to uridine and guanosine-rich RNAs (Ludevid et al., 1992). Regulation studies indicated that MA16 mRNA had a basal level of expression in several tissues including embryos and seedlings. We also showed that the level of mRNA in RNA gel blots increa ...
Shaping the Endoplasmic Reticulum into a Social Network
... ER fusogens ATL and Sey1p/RHD3, the microtubule-severing protein spastin, and the ER morphology regulators Lnp and protrudin[1_TD$IF] (Figure 1). Two mechanisms have been proposed for curvature generation in ER tubules by tubule-forming proteins: the insertion of RHD wedges and scaffold formation vi ...
... ER fusogens ATL and Sey1p/RHD3, the microtubule-severing protein spastin, and the ER morphology regulators Lnp and protrudin[1_TD$IF] (Figure 1). Two mechanisms have been proposed for curvature generation in ER tubules by tubule-forming proteins: the insertion of RHD wedges and scaffold formation vi ...
AHM1, a Novel Type of Nuclear Matrix–Localized
... (A) An alignment of J domains. J domain proteins can be divided into three groups: DnaJ/Hsp40, which includes the canonical DnaJ and closely related homologs; J-Z array, which includes AHM1 and selected Arabidopsis proteins (shown with their GenBank accession numbers) having the Zn finger–like motif ...
... (A) An alignment of J domains. J domain proteins can be divided into three groups: DnaJ/Hsp40, which includes the canonical DnaJ and closely related homologs; J-Z array, which includes AHM1 and selected Arabidopsis proteins (shown with their GenBank accession numbers) having the Zn finger–like motif ...
Power Point presentation
... Preliminary results show that the in vivo method of testing is feasible and practical. The resulting data will allow concrete trends in chaperone activity to be established. ...
... Preliminary results show that the in vivo method of testing is feasible and practical. The resulting data will allow concrete trends in chaperone activity to be established. ...
Protein Denaturation Studies Using the Pyris 6 DSC
... With the increasing focus upon biotechnological applications of thermal analysis, the use of high sensitivity DSC instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional st ...
... With the increasing focus upon biotechnological applications of thermal analysis, the use of high sensitivity DSC instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional st ...
Nuclear Transport of Plant Potyviral Proteins
... p.i. and remained at relatively constant or slightly decreased levels for an additional 3 days (data not shown). lmmunocytochemical analysis of TEV-infected tissue revealed virus activity in most cells by 3 days p.i. (see below), suggesting that accumulation between days 3 and 5 was due to increases ...
... p.i. and remained at relatively constant or slightly decreased levels for an additional 3 days (data not shown). lmmunocytochemical analysis of TEV-infected tissue revealed virus activity in most cells by 3 days p.i. (see below), suggesting that accumulation between days 3 and 5 was due to increases ...
Section N – Regulation of transcription in eukaryotes
... myoD also activates expression of p21waf1/cip1 expression, a small molecule inhibitor of CDKs, causing cells arrested at the G1-phase of the cell cycle which is characteristic of differentiated cells. . ...
... myoD also activates expression of p21waf1/cip1 expression, a small molecule inhibitor of CDKs, causing cells arrested at the G1-phase of the cell cycle which is characteristic of differentiated cells. . ...
Introduction Part1
... reduced affinity to microtubule): Trailing head swings by ~ 160 Å, net movement of dimeric kinesin is ~ 80 Å = length of one microtubule dimer ATP in new trailing head is hydrolyzed & phosphate released: affinity for microtuble decreases ADP in new leading head dissociates ...
... reduced affinity to microtubule): Trailing head swings by ~ 160 Å, net movement of dimeric kinesin is ~ 80 Å = length of one microtubule dimer ATP in new trailing head is hydrolyzed & phosphate released: affinity for microtuble decreases ADP in new leading head dissociates ...
SILAC and iTRAQ Quantitation on an Orbitrap Using Protein
... 1. SILAC experiment: The 26S proteasome complex was purified from cells expressing Rpn11-TAP. The cells grown in heavy media containing 13C,15N arg/lys were used as a control and the cells grown in light media containing 12C,14N-arg/lys were treated with hydrogen peroxide. The cells were lysed and m ...
... 1. SILAC experiment: The 26S proteasome complex was purified from cells expressing Rpn11-TAP. The cells grown in heavy media containing 13C,15N arg/lys were used as a control and the cells grown in light media containing 12C,14N-arg/lys were treated with hydrogen peroxide. The cells were lysed and m ...
Atomic contacts in protein structures. A detailed analysis of atomic
... criterion for the assessment of potential ligands in molecular docking programs.26,27 Accurate atomic radii together with a robust evaluation of packing can therefore be expected to significantly enhance the virtual screening of protein–ligand complexes. In addition to structure, dynamics is often c ...
... criterion for the assessment of potential ligands in molecular docking programs.26,27 Accurate atomic radii together with a robust evaluation of packing can therefore be expected to significantly enhance the virtual screening of protein–ligand complexes. In addition to structure, dynamics is often c ...
AutoDock Parameters docking procedures The AutoDock program is
... The probe peptide torsion angles are set to be flexible by AUTOTORS utility of AutoDock. The polar hydrogens are added to the protein and the peptide; Gasteiger charges are added by AutoDock Tools: ADT. Genetic Algorithm option for the docking is selected. Lamarckian Genetic Algorithm is chosen as t ...
... The probe peptide torsion angles are set to be flexible by AUTOTORS utility of AutoDock. The polar hydrogens are added to the protein and the peptide; Gasteiger charges are added by AutoDock Tools: ADT. Genetic Algorithm option for the docking is selected. Lamarckian Genetic Algorithm is chosen as t ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.