Solid Tumour Section Ovary: inv(10)(q11q11) in ovarian germ cell tumors
... Diagrammatic representation of RET/NCOA4 (PTC3) fusion protein. Amino acids surrounding each breakpoint (arrows) are indicated, and numbered according to their position within RET or NCOA4, respectively. Peptide sequences from RET are in red, from NCOA4 in green. The transmembrane domain (TM) of RET ...
... Diagrammatic representation of RET/NCOA4 (PTC3) fusion protein. Amino acids surrounding each breakpoint (arrows) are indicated, and numbered according to their position within RET or NCOA4, respectively. Peptide sequences from RET are in red, from NCOA4 in green. The transmembrane domain (TM) of RET ...
Challenges in characterising biopharmaceuticals for ease of
... 2. Breakout Group 1 Protein degradation of final dosage form. Need assay(s) that work with very low amounts of material, combine assays to get more information, develop platform assays. Assays need to provide information on site specificity Assays need to be able to predict final performance of dosa ...
... 2. Breakout Group 1 Protein degradation of final dosage form. Need assay(s) that work with very low amounts of material, combine assays to get more information, develop platform assays. Assays need to provide information on site specificity Assays need to be able to predict final performance of dosa ...
EXAMINATIONOFTHESUBUNITSOFHYN HYDROGENASE INT
... the strain. The next generation 454 method was combined with 30556 sequencing reads obtained from these libraries by Sanger method. This hybrid approach yielded around 98% of the T. roseopersicina’s total genome. ...
... the strain. The next generation 454 method was combined with 30556 sequencing reads obtained from these libraries by Sanger method. This hybrid approach yielded around 98% of the T. roseopersicina’s total genome. ...
Determining Lactose Content In Milk by Polarimetry
... polarimeter tube. It is affected by temperature (20°C reference temperature) and wavelength (usually the sodium D line, 589 nm, is used), and can be measured using the following formula: ...
... polarimeter tube. It is affected by temperature (20°C reference temperature) and wavelength (usually the sodium D line, 589 nm, is used), and can be measured using the following formula: ...
Amino Acids, Peptides and Proteins
... 3. A second BOC-protected amino acid is coupled to the first one using DCC. The beads are washed. 4. The cycle of deprotection, coupling and washing is repeated asmany times as desired to add amino acid units to the growing chain. 5. After the desired peptide has been made, the treatment with anhydr ...
... 3. A second BOC-protected amino acid is coupled to the first one using DCC. The beads are washed. 4. The cycle of deprotection, coupling and washing is repeated asmany times as desired to add amino acid units to the growing chain. 5. After the desired peptide has been made, the treatment with anhydr ...
Print
... structure of the protein. Linear polypeptide chains are often cross-linked, most commonly by two cysteine residues linked together to form a cystine unit. 2. Secondary structure: The folding of a polypeptide backbone by means of internal hydrogen bonds between nearby amino acid residues giving rise ...
... structure of the protein. Linear polypeptide chains are often cross-linked, most commonly by two cysteine residues linked together to form a cystine unit. 2. Secondary structure: The folding of a polypeptide backbone by means of internal hydrogen bonds between nearby amino acid residues giving rise ...
Functional analysis of the Drosophila Dnop5 gene using targeted RNA interference
... Dnop5, a novel gene in Drosophila, is the member of the conserved nop5/sik1 gene family. As other Nop5/Sik1 family members, DNop5 contains multiple KKX motifs at the carboxy terminus, and is the closest relative of Nop5 of Caenorhabditis elegans. Dnop5 cDNA codes for a transcript of approximately 18 ...
... Dnop5, a novel gene in Drosophila, is the member of the conserved nop5/sik1 gene family. As other Nop5/Sik1 family members, DNop5 contains multiple KKX motifs at the carboxy terminus, and is the closest relative of Nop5 of Caenorhabditis elegans. Dnop5 cDNA codes for a transcript of approximately 18 ...
Multivalent cross-linking of actin filaments and microtubules through
... Microtubule-associated proteins regulate microtubule dynamics, bundle actin filaments and cross-link actin filaments with microtubules. In addition, aberrant interaction of the microtubule-associated protein Tau with filamentous actin is connected to synaptic impairment in Alzheimer’s disease. Here ...
... Microtubule-associated proteins regulate microtubule dynamics, bundle actin filaments and cross-link actin filaments with microtubules. In addition, aberrant interaction of the microtubule-associated protein Tau with filamentous actin is connected to synaptic impairment in Alzheimer’s disease. Here ...
Sourirajan, Jayanthi: Protein Structure Prediction
... Proteins are building blocks of life. Proteins exhibit more sequence and chemical complexity than DNA or RNA. A protein sequence is a linear hetero polymer made up of one of the 20 different amino acids. They perform a wide variety of functions in the living organism, playing various catalytic, stru ...
... Proteins are building blocks of life. Proteins exhibit more sequence and chemical complexity than DNA or RNA. A protein sequence is a linear hetero polymer made up of one of the 20 different amino acids. They perform a wide variety of functions in the living organism, playing various catalytic, stru ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... involved in DNA replication and gene expression (Lamond and Earnshaw, 1998). The most prominent nuclear subcompartment is the nucleolus, which is the site of transcription and processing of ribosomal RNAs (rRNAs), and their assembly into ribosomal subunits before export to the cytoplasm (Olsen et al ...
... involved in DNA replication and gene expression (Lamond and Earnshaw, 1998). The most prominent nuclear subcompartment is the nucleolus, which is the site of transcription and processing of ribosomal RNAs (rRNAs), and their assembly into ribosomal subunits before export to the cytoplasm (Olsen et al ...
Analysis of Proteins
... • Respiration – energy that is made in cells through a complex series of oxidation reactions • Bomb Calorimeter – special type of calorimeter used to measure heat of combustion of certain reactions – can also measure energy in food. ...
... • Respiration – energy that is made in cells through a complex series of oxidation reactions • Bomb Calorimeter – special type of calorimeter used to measure heat of combustion of certain reactions – can also measure energy in food. ...
Structure and Function of the Groucho Gene Family and Encoded
... TTTCAG in the genomic sequence abutting the short 5’ untranslated region TAAAA (Pflugrad et al., 1997). In humans, there are four Groucho (TLE1, TLE2, TLE3 and TLE4) genes and one AES gene. We previously reported that human TLE3 and AES genes are located on human chromosomes 15 and 19, respectively ...
... TTTCAG in the genomic sequence abutting the short 5’ untranslated region TAAAA (Pflugrad et al., 1997). In humans, there are four Groucho (TLE1, TLE2, TLE3 and TLE4) genes and one AES gene. We previously reported that human TLE3 and AES genes are located on human chromosomes 15 and 19, respectively ...
Paul McCain Presentation
... (mitochondrial fission), Bak (cellular apoptosis) and Tom20 (protein import) also reduced but over a longer time period Intermembrane and matrix proteins of mitochondria showed no significant changes Opa1 has short and long isoforms. The long isoform (outer membrane) is reduced while the short isofo ...
... (mitochondrial fission), Bak (cellular apoptosis) and Tom20 (protein import) also reduced but over a longer time period Intermembrane and matrix proteins of mitochondria showed no significant changes Opa1 has short and long isoforms. The long isoform (outer membrane) is reduced while the short isofo ...
Protein folding
... called amyloid may form. Formation of these aggregates in vivo is strongly restricted by the chaperone machinery. Molecular chaperone interact with, stabilize or help proteins to acquire its functionally active conformation, without being present in its final structure. Different classes of structur ...
... called amyloid may form. Formation of these aggregates in vivo is strongly restricted by the chaperone machinery. Molecular chaperone interact with, stabilize or help proteins to acquire its functionally active conformation, without being present in its final structure. Different classes of structur ...
GPCRs10
... • However, the exact aa sequence responsible for this has been difficult to pinpoint. ...
... • However, the exact aa sequence responsible for this has been difficult to pinpoint. ...
Williams, Brandon: A Review on Common Applications Designed to Optimize the SEQUEST Search Algorithm With Respect to Limiting False Positive Identification
... New proteomic technologies have emerged that deal with understanding complex systems and diseases at a global level, thus allowing an expanded experimental view. Before the advent of mass spectrometry, proteomic approaches were initially explored via the use of two-dimensional gel electrophoresis (1 ...
... New proteomic technologies have emerged that deal with understanding complex systems and diseases at a global level, thus allowing an expanded experimental view. Before the advent of mass spectrometry, proteomic approaches were initially explored via the use of two-dimensional gel electrophoresis (1 ...
Tailor-Made Proteins and Peptides for Quantum Interference
... The sublimability of molecules is increased by reducing their intermolecular interactions and thus, peptides exposing fluorinated groups are at the focus of interest. As a starting point, lysine rich model peptides were selected for the decoration with fluorous tags. The exposed primary amino group ...
... The sublimability of molecules is increased by reducing their intermolecular interactions and thus, peptides exposing fluorinated groups are at the focus of interest. As a starting point, lysine rich model peptides were selected for the decoration with fluorous tags. The exposed primary amino group ...
Selected reaction monitoring applied to proteomics
... three abundant proteins.[16] The measurements performed by SRM were compared with results from immunoassays, which appeared to be closely correlated. Similarly, prostate-specific antigen was also directly measured in non-depleted serum.[17] These examples demonstrated the potential of SID–SRM analys ...
... three abundant proteins.[16] The measurements performed by SRM were compared with results from immunoassays, which appeared to be closely correlated. Similarly, prostate-specific antigen was also directly measured in non-depleted serum.[17] These examples demonstrated the potential of SID–SRM analys ...
Diseases of the Immune System lec.4
... Amyloidosis is subdivided into systemic (generalized) and localized (tissuespecific) forms and is further classified on the basis of predisposing conditions. Systemic amyloidosis is associated with the following conditions: 1. Immunocyte Dyscrasias with Amyloidosis Amyloid in this category usually i ...
... Amyloidosis is subdivided into systemic (generalized) and localized (tissuespecific) forms and is further classified on the basis of predisposing conditions. Systemic amyloidosis is associated with the following conditions: 1. Immunocyte Dyscrasias with Amyloidosis Amyloid in this category usually i ...
Recombinant Human PTH
... the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor a ...
... the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor a ...
Printer Friendly Document
... * Select Lactococcus lactis MG1363 from organism list (results are similar but not identical using other species) * Click Go! * Displays ‘Evidence View’ - different line colors represent types of association (clustering on chromosome (= Neighborhood), co-occurrence, co-expression, protein-protein in ...
... * Select Lactococcus lactis MG1363 from organism list (results are similar but not identical using other species) * Click Go! * Displays ‘Evidence View’ - different line colors represent types of association (clustering on chromosome (= Neighborhood), co-occurrence, co-expression, protein-protein in ...
Leukaemia Section t(9;22)(q34;q11) in ALL Atlas of Genetics and Cytogenetics
... - breakpoint in ABL is variable over a region of 200 kb, often between the two alternative exons 1b and 1a, sometimes 5' of 1b, or 3' of 1a, but always 5' of exon 2; - breakpoint in BCR is either: 1- in the same region as in CML, called M-bcr (for major breakpoint cluster region), a cluster of 5.8 k ...
... - breakpoint in ABL is variable over a region of 200 kb, often between the two alternative exons 1b and 1a, sometimes 5' of 1b, or 3' of 1a, but always 5' of exon 2; - breakpoint in BCR is either: 1- in the same region as in CML, called M-bcr (for major breakpoint cluster region), a cluster of 5.8 k ...
Fibrous proteins
... Elastin is an insoluble protein polymer synthesized from a precursor, TROPOELASTIN, which is a linear polypeptide composed of about 700 amino acids that are primarily small and nonpolar (for example, glycine, alanine, and valine). Tropoelastin is secreted by the cell into the extracellular space. Th ...
... Elastin is an insoluble protein polymer synthesized from a precursor, TROPOELASTIN, which is a linear polypeptide composed of about 700 amino acids that are primarily small and nonpolar (for example, glycine, alanine, and valine). Tropoelastin is secreted by the cell into the extracellular space. Th ...
lecture 15
... ITC data shows Hsp70/Hsp90 preference for TPR1 or TPR2A binding sites n (ratio of binding) is ~1 for all Kd measured (lower μM means tighter binding) ...
... ITC data shows Hsp70/Hsp90 preference for TPR1 or TPR2A binding sites n (ratio of binding) is ~1 for all Kd measured (lower μM means tighter binding) ...
2 a - Atelier de BioInformatique
... • Cliques of “symbols” define similarity, e.g : - different overlapping sets of amino acids clustered by their properties (e.g. hydrophobic, hydrophilic, small, large, polar, charged, etc…) - different overlapping sets of amino acids clustered by setting a threshold value on their score in a similar ...
... • Cliques of “symbols” define similarity, e.g : - different overlapping sets of amino acids clustered by their properties (e.g. hydrophobic, hydrophilic, small, large, polar, charged, etc…) - different overlapping sets of amino acids clustered by setting a threshold value on their score in a similar ...
Proteomics
Proteomics is the large-scale study of proteins, particularly their structures and functions. Proteins are vital parts of living organisms, as they are the main components of the physiological metabolic pathways of cells. The term proteomics was first coined in 1997 to make an analogy with genomics, the study of the genome. The word proteome is a portmanteau of protein and genome, and was coined by Marc Wilkins in 1994 while working on the concept as a PhD student.The proteome is the entire set of proteins, produced or modified by an organism or system. This varies with time and distinct requirements, or stresses, that a cell or organism undergoes. Proteomics is an interdisciplinary domain formed on the basis of the research and development of the Human Genome Project; it is also emerging scientific research and exploration of proteomes from the overall level of intracellular protein composition, structure, and its own unique activity patterns. It is an important component of functional genomics.While proteomics generally refers to the large-scale experimental analysis of proteins, it is often specifically used for protein purification and mass spectrometry.