Kinetic Role of Helix Caps in Protein Folding Is Context
... proper cellular function. If the native state is the functional species, then that function is lost during any time spent in the unfolded state or in the folding process. The denatured or unfolded ensemble of structures may be more susceptible to protein turnover through cellular degradation pathway ...
... proper cellular function. If the native state is the functional species, then that function is lost during any time spent in the unfolded state or in the folding process. The denatured or unfolded ensemble of structures may be more susceptible to protein turnover through cellular degradation pathway ...
Towards the storage metabolome: profiling the barley vacuole
... are found in locations other than those they were thought to operate for example hexokinase proteins have been found in the nucleus whilst the sucrose synthase has been localized to the mitochondria (Subbaiah et al., 2006; Cho et al., 2007). These observations thus render such predictions of metabol ...
... are found in locations other than those they were thought to operate for example hexokinase proteins have been found in the nucleus whilst the sucrose synthase has been localized to the mitochondria (Subbaiah et al., 2006; Cho et al., 2007). These observations thus render such predictions of metabol ...
12. Molecular Recognition: The Thermodynamics of
... From The Molecules of Life by Kuriyan, Konforti & Wemmer © Garland Publishing, 2009. Distribution Prohibited. ...
... From The Molecules of Life by Kuriyan, Konforti & Wemmer © Garland Publishing, 2009. Distribution Prohibited. ...
Development of antifertility vaccine using sperm specific proteins
... presence of antibodies may impair the fertilization process. the presence of antisperm antibodies has been shown to be associated with the individuals who are immunologically infertile but they are otherwise normal. Therefore, the use of sperm proteins has been considered as one of the promising app ...
... presence of antibodies may impair the fertilization process. the presence of antisperm antibodies has been shown to be associated with the individuals who are immunologically infertile but they are otherwise normal. Therefore, the use of sperm proteins has been considered as one of the promising app ...
Regulation of pyruvate, orthophosphate dikinase by ADP
... enzyme activity and phosphorylatability (Table 2). Alternatively, the inactivation mechanism may also include a steric component in addition to an electrostatic one since substitution of Thr by Tyr or Phe (but not Val), two neutral amino acids with bulky side chains, also dramatically inhibits dikin ...
... enzyme activity and phosphorylatability (Table 2). Alternatively, the inactivation mechanism may also include a steric component in addition to an electrostatic one since substitution of Thr by Tyr or Phe (but not Val), two neutral amino acids with bulky side chains, also dramatically inhibits dikin ...
2.3 Carbon-Based Molecules
... 2.3 Carbon-Based Molecules • Proteins are polymers of amino acid monomers. – Twenty different amino acids are used to build proteins in organisms. – Amino acids differ in side groups, or R groups. – Amino acids are linked by peptide bonds. ...
... 2.3 Carbon-Based Molecules • Proteins are polymers of amino acid monomers. – Twenty different amino acids are used to build proteins in organisms. – Amino acids differ in side groups, or R groups. – Amino acids are linked by peptide bonds. ...
Fatty Acid Biosynthesis
... Malonyl-CoA carbons become new COOH end Nascent chain remains tethered to ACP CO2, HS-CoA are released at each condensation ...
... Malonyl-CoA carbons become new COOH end Nascent chain remains tethered to ACP CO2, HS-CoA are released at each condensation ...
Document
... Unfortunately, most of the candidate techniques like X-ray crystallography, electron microscopy and solid-state NMR spectroscopy, are unsuitable for in vivo applications due to their requirements for pure samples, prohibitively large sample concentrations, and crystalline or vitrified specimens. Liq ...
... Unfortunately, most of the candidate techniques like X-ray crystallography, electron microscopy and solid-state NMR spectroscopy, are unsuitable for in vivo applications due to their requirements for pure samples, prohibitively large sample concentrations, and crystalline or vitrified specimens. Liq ...
Capillary Electrophoresis of Proteins
... – Higher concentrations can result in tailing peaks due to insufficient protein binding ...
... – Higher concentrations can result in tailing peaks due to insufficient protein binding ...
Do Water Molecules Mediate Protein-DNA Recognition?
... The crystal structure of the complex of tRNAGln and its cognate tRNA synthetase shows that water molecules occur in the minor groove of the doublestranded RNA helix of the tRNA, near the amino acid acceptor stem, and may help to establish the tRNA's identity.36 ...
... The crystal structure of the complex of tRNAGln and its cognate tRNA synthetase shows that water molecules occur in the minor groove of the doublestranded RNA helix of the tRNA, near the amino acid acceptor stem, and may help to establish the tRNA's identity.36 ...
Systematic Characterisation of Cellular Localisation and
... Expression analysis - proteins containing MHC ligands have elevated mRNA levels Next, we analysed the expression profile of proteins containing MHC class I or II ligands based on their mRNA abundance as determined by DNA microarray analysis obtained from the GNF gene expression database [18]. The da ...
... Expression analysis - proteins containing MHC ligands have elevated mRNA levels Next, we analysed the expression profile of proteins containing MHC class I or II ligands based on their mRNA abundance as determined by DNA microarray analysis obtained from the GNF gene expression database [18]. The da ...
Chromosomal rearrangements and protein globularity changes in
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
... PPE53 and PPE24) were found in at least four of the strains. The variants in eight of these genes led to amino acid changes but only two altered genes have known functions: PE_PGRS19, a putative outer membrane protein (Song et al., 2008) and embR which is involved in transcription, the biosynthesis ...
Crude protein and amino acids content in some common
... found in the EP2® pellet. There was significantly difference between EP2® pellet with other diets (p<0.05), however, no significantly differences were observed among the EP2® pellet shrimps, Love Larva® pellet and anchovies for Lysine (p>0.05), however, these values were significantly different than ...
... found in the EP2® pellet. There was significantly difference between EP2® pellet with other diets (p<0.05), however, no significantly differences were observed among the EP2® pellet shrimps, Love Larva® pellet and anchovies for Lysine (p>0.05), however, these values were significantly different than ...
Chapter 2: Biochemistry Problems
... cannot form hydrophobic interactions. Unlike an ionic or a hydrogen bond that occurs between two molecules, hydrophobic interactions are not true bonds, but involve nonpolar molecules that cluster together to avoid the water that surrounds them. The effect of clustering nonpolar molecules or chemica ...
... cannot form hydrophobic interactions. Unlike an ionic or a hydrogen bond that occurs between two molecules, hydrophobic interactions are not true bonds, but involve nonpolar molecules that cluster together to avoid the water that surrounds them. The effect of clustering nonpolar molecules or chemica ...
Mutational Effect to Particular Interaction Energy of Cycloguanil Drug
... mechanic minimization was firstly performed to optimize the complex structures. The Cyc structure was fully optimized at the B3LYP/6-31G(*) level of calculations, and then the RESP electrostatic charges of all atoms in the Cyc inhibitor were also calculated using the Gaussian 03 package. The resulta ...
... mechanic minimization was firstly performed to optimize the complex structures. The Cyc structure was fully optimized at the B3LYP/6-31G(*) level of calculations, and then the RESP electrostatic charges of all atoms in the Cyc inhibitor were also calculated using the Gaussian 03 package. The resulta ...
108. Regulation of the L-arabinose Operon in Escherichia coli, in
... AraC protein prefers to loop in the absence of arabinose because an N-terminal arm of about 15 amino acids extends from the dimerization domain and binds to the DNAbinding domain. The combination of the direct connection between the dimerization domains and the DNA-binding domains and the noncovalen ...
... AraC protein prefers to loop in the absence of arabinose because an N-terminal arm of about 15 amino acids extends from the dimerization domain and binds to the DNAbinding domain. The combination of the direct connection between the dimerization domains and the DNA-binding domains and the noncovalen ...
Authors Title Year Keywords Journal/Proceedings Emile Bol
... Abstract: Although the folding of {alpha}-helical repeat proteins has been well characterized, much less is known about the folding of repeat proteins containing β-sheets. Here we investigate the folding thermodynamics and kinetics of the leucine-rich repeat (LRR) domain of Internalin B (InlB), an e ...
... Abstract: Although the folding of {alpha}-helical repeat proteins has been well characterized, much less is known about the folding of repeat proteins containing β-sheets. Here we investigate the folding thermodynamics and kinetics of the leucine-rich repeat (LRR) domain of Internalin B (InlB), an e ...
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
CHAPTER 6 Gene Expression: Translation
... 2. Two or more polypeptide chains may associate to form a protein complex. Each cell type has characteristic proteins that are associated with its function. 3. All amino acids (except proline) have a common structure (Figure 6.1). a. The α-carbon is bonded to: i. An amino group (NH2), which is usual ...
... 2. Two or more polypeptide chains may associate to form a protein complex. Each cell type has characteristic proteins that are associated with its function. 3. All amino acids (except proline) have a common structure (Figure 6.1). a. The α-carbon is bonded to: i. An amino group (NH2), which is usual ...
Lecture 38 - Amino Acid Metabolism 1
... intestine where proteases cleave the peptide bond to yield amino acids and small oligopeptides. ...
... intestine where proteases cleave the peptide bond to yield amino acids and small oligopeptides. ...
Zinc finger family
... Coactivator proteins CBP and p300 contain two copies of a zinc finger motif, termed the TAZ finger, that are implicated in functional interactions with numerous transcription factors and viral oncoproteins.The TAZ2 zinc finger folds into an unusual bundle of four helices that is stabilized by three ...
... Coactivator proteins CBP and p300 contain two copies of a zinc finger motif, termed the TAZ finger, that are implicated in functional interactions with numerous transcription factors and viral oncoproteins.The TAZ2 zinc finger folds into an unusual bundle of four helices that is stabilized by three ...
The stability and nuclear localization of the transcription factor RAP2
... proteins and lipids is reduced (Geigenberger 2003). Moreover, energy-saving pathways are favoured over those that are more expensive in terms of ATP (Bologa et al. 2003). When oxygen becomes limiting for oxidative phosphorylation, ATP production relies on fermentative metabolism. Its activation coin ...
... proteins and lipids is reduced (Geigenberger 2003). Moreover, energy-saving pathways are favoured over those that are more expensive in terms of ATP (Bologa et al. 2003). When oxygen becomes limiting for oxidative phosphorylation, ATP production relies on fermentative metabolism. Its activation coin ...
Multidrug resistance mediated by the ATP-binding
... Sequences were aligned along their entire length with MRP using CLUSTAL W(1.6) multiple sequence alignment. Sequence data were obtained using the following accession numbers: MRP, L05628/P33527; mrp, AF022908/1488428; MOAT, U49248/U63970; EBCR, 1430907/Z49144; C. elegans mrp1, U66260; C. elegans mrp ...
... Sequences were aligned along their entire length with MRP using CLUSTAL W(1.6) multiple sequence alignment. Sequence data were obtained using the following accession numbers: MRP, L05628/P33527; mrp, AF022908/1488428; MOAT, U49248/U63970; EBCR, 1430907/Z49144; C. elegans mrp1, U66260; C. elegans mrp ...
The Cutting Edge of Affinity Electrophoresis Technology
... compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cells. It has been reported that changes in the structures of the sugar chains of mucins a ...
... compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cells. It has been reported that changes in the structures of the sugar chains of mucins a ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.