1MBO Lopez kin
... R115L mutation that has no measurable effect on kinetic parameters. The human enzyme exists as a homodimer with a molecular mass of 86 kDa. Each subunit (Fig. 1a) contains residues 65−423 (GenBank D00726) and one [2Fe-2S] cluster, while residues 1−62 represent a mitochondrial targeting sequence that ...
... R115L mutation that has no measurable effect on kinetic parameters. The human enzyme exists as a homodimer with a molecular mass of 86 kDa. Each subunit (Fig. 1a) contains residues 65−423 (GenBank D00726) and one [2Fe-2S] cluster, while residues 1−62 represent a mitochondrial targeting sequence that ...
... Choice B: The version of Phosphofructose kinase (PFK) in the muscle is different than that from the liver. Although both catalyze the same reaction, they are regulated differently. Based on your knowledge of PFK in the liver, and your knowledge of liver and muscle function, suggest how PFK in the mu ...
03_Krivov
... evaluate function at these points 2. Assume that second order approximation works well ...
... evaluate function at these points 2. Assume that second order approximation works well ...
G PROTEIN βγ SUBUNITS
... other proteins that make up the WD-repeat superfamily. Members of this family do not have an immediately obvious common function; they are involved in diverse cellular pathways such as signal transduction, pre-mRNA splicing, transcriptional regulation, assembly of the cytoskeleton, and vesicular tra ...
... other proteins that make up the WD-repeat superfamily. Members of this family do not have an immediately obvious common function; they are involved in diverse cellular pathways such as signal transduction, pre-mRNA splicing, transcriptional regulation, assembly of the cytoskeleton, and vesicular tra ...
Heterotrimeric G Protein–Coupled Signaling in Plants
... GTPase-accelerating proteins (GAPs)—specifically known as regulator of G protein signaling (RGS) proteins—speed deactivation (96). There are at least 37 RGS proteins in humans, falling into 10 basic architectures, none of which contain transmembrane domains, although many contain domains that permit ...
... GTPase-accelerating proteins (GAPs)—specifically known as regulator of G protein signaling (RGS) proteins—speed deactivation (96). There are at least 37 RGS proteins in humans, falling into 10 basic architectures, none of which contain transmembrane domains, although many contain domains that permit ...
OCAM: A new tool for studying the oligomeric diversity of MscL
... Supporting Information Figure S5. Detergent dependence of the OCAM reaction for EcMscL. EcMScLsGFP was solubilized, purified, and measured by OCAM in the detergents C12E8 (Anatrace, polyoxyethylene(8)dodecyl ether) and DDM (Anatrace, n-dodecyl-β-D-maltopyranoside). The DDM sample migrates as two oli ...
... Supporting Information Figure S5. Detergent dependence of the OCAM reaction for EcMscL. EcMScLsGFP was solubilized, purified, and measured by OCAM in the detergents C12E8 (Anatrace, polyoxyethylene(8)dodecyl ether) and DDM (Anatrace, n-dodecyl-β-D-maltopyranoside). The DDM sample migrates as two oli ...
Somatic cancer mutations in the MLL1 histone methyltransferase
... of histones (H3 and H4) and other proteins (Cheng et al., 2005; Clarke, 2013; Del Rizzo and Trievel, 2014; Dillon et al., 2005; Kudithipudi and Jeltsch, 2016; Zhang et al., 2015) and thereby play an important role in gene expression, cellular development and many diseases including cancer (Chi et al ...
... of histones (H3 and H4) and other proteins (Cheng et al., 2005; Clarke, 2013; Del Rizzo and Trievel, 2014; Dillon et al., 2005; Kudithipudi and Jeltsch, 2016; Zhang et al., 2015) and thereby play an important role in gene expression, cellular development and many diseases including cancer (Chi et al ...
Enzymatic Protein Deglycosylation Kit (EDEGLY)
... well as sialic acid.14,15 Complete removal of this O-linked structure or its derivatives would require, in addition to neuraminidase, a β(1→4)-specific galactosidase and an N-acetylglucosaminidase. The galactosidase must be β(1→4)-specific since a non-specific galactosidase would remove the β(1→3)-g ...
... well as sialic acid.14,15 Complete removal of this O-linked structure or its derivatives would require, in addition to neuraminidase, a β(1→4)-specific galactosidase and an N-acetylglucosaminidase. The galactosidase must be β(1→4)-specific since a non-specific galactosidase would remove the β(1→3)-g ...
Protein Structure - Macmillan Learning
... Part of the explanation of how proteins fold lies in their reaction to an aqueous environment. Most proteins reside in the cell’s aqueous cytoplasm, yet many amino acids are hydrophobic, or water-fearing. Hydrophobic residues, scattered throughout the length of a protein, tend to gather together, th ...
... Part of the explanation of how proteins fold lies in their reaction to an aqueous environment. Most proteins reside in the cell’s aqueous cytoplasm, yet many amino acids are hydrophobic, or water-fearing. Hydrophobic residues, scattered throughout the length of a protein, tend to gather together, th ...
hydrocarbon chains
... 4. This region is monitored. 5. Bleached molecules leave and unbleached molecules enter. 6. Recover the fluorescent intensity in this region. ...
... 4. This region is monitored. 5. Bleached molecules leave and unbleached molecules enter. 6. Recover the fluorescent intensity in this region. ...
Delivering of Proteins to the Plant Vacuole—An Update
... For all eukaryotic cells, the transport from the Golgi apparatus to the vacuole or lysosomes requires positive information from the protein. In mammals, the signal to transport proteins to the lysosome is the addition of a mannose 6-phosphate residue during the passage through the Golgi complex, whi ...
... For all eukaryotic cells, the transport from the Golgi apparatus to the vacuole or lysosomes requires positive information from the protein. In mammals, the signal to transport proteins to the lysosome is the addition of a mannose 6-phosphate residue during the passage through the Golgi complex, whi ...
The relative importance of intracellular proteolysis and
... required are hydrolyzed in energy– dependent reactions catalyzed by intracellular proteinases [2]. Cells contain a large number of endopeptidases that are localized in the cytoplasm, periplasm and cytoplasmic membrane [3,4]. In addition to endopeptidases, exopeptidases further degrade the peptides g ...
... required are hydrolyzed in energy– dependent reactions catalyzed by intracellular proteinases [2]. Cells contain a large number of endopeptidases that are localized in the cytoplasm, periplasm and cytoplasmic membrane [3,4]. In addition to endopeptidases, exopeptidases further degrade the peptides g ...
medical management: portosystemic vascular anomalies (psva)
... astrocytes (type of glial cell) are the only brain cells equipped for ammonia metabolism (glutamine synthetase catalyzes conversion of NH3 glutamine). In HE, these cells undergo functional and structural alterations increasing in size and number, predominantly in the cerebral cortex, cerebellum an ...
... astrocytes (type of glial cell) are the only brain cells equipped for ammonia metabolism (glutamine synthetase catalyzes conversion of NH3 glutamine). In HE, these cells undergo functional and structural alterations increasing in size and number, predominantly in the cerebral cortex, cerebellum an ...
galactose specific
... Legume lectins: peanut lectin, winged bean basic and acidic lectins and their complexes • Multimeric proteins can assume open quaternary structures • High variability in quaternary structure while the tertiary structure is essentially same • Water bridges as a strategy for generating ligand specifi ...
... Legume lectins: peanut lectin, winged bean basic and acidic lectins and their complexes • Multimeric proteins can assume open quaternary structures • High variability in quaternary structure while the tertiary structure is essentially same • Water bridges as a strategy for generating ligand specifi ...
hydrolysis of keratin materials derived from poultry industry
... Weight of calcium hydroxide added to the feathers (0.05-0.75 g), the mass of water (2.0-5.0 g), the temperature of the second stage of the process (90-110°C), its duration (60-240 min) were input quantities that describe the test object (the process of alkaline hydrolysis feathers). The feather mass ...
... Weight of calcium hydroxide added to the feathers (0.05-0.75 g), the mass of water (2.0-5.0 g), the temperature of the second stage of the process (90-110°C), its duration (60-240 min) were input quantities that describe the test object (the process of alkaline hydrolysis feathers). The feather mass ...
SpyTag and SpyCatcher - Department of Biochemistry
... even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is also a split protein (and may have dynamic tertiary structure), so even though SpyCatcher is a satisfactory fusion tag in different proteins using bacterial and mammalian expression, there can still be a reduction in expressi ...
... even after we reduced the size from 116 to 84 residues [30]. SpyCatcher is also a split protein (and may have dynamic tertiary structure), so even though SpyCatcher is a satisfactory fusion tag in different proteins using bacterial and mammalian expression, there can still be a reduction in expressi ...
Full_ppt_ch20
... • Proteins are polymers of amino acids • Contain a carboxylic acid group and an amino group on the alpha carbon • Side group R gives unique characteristics R ...
... • Proteins are polymers of amino acids • Contain a carboxylic acid group and an amino group on the alpha carbon • Side group R gives unique characteristics R ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
... supernatant was similar and the starch synthase activity eluted at the same positions in the gradient (not illustrated). Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubil ...
... supernatant was similar and the starch synthase activity eluted at the same positions in the gradient (not illustrated). Another factor that did not affect recovery of activity or the activity profile obtained from the Mono-Q chromatography was whether the purified starch was used 'wet/ i.e. solubil ...
Structure of the Gene Coding for the a Polypeptide Chain of
... transcription in liver, or in Hep-G2 cells, this gene produces a single transcript of 2,262 nucleotides, excepting the poly A tail, that presents an unusually long 5' untranslated region (5' UTR) of 223 nucleotides . The C4BPa gene is organized as follows : the first exon codes for the first 198 nuc ...
... transcription in liver, or in Hep-G2 cells, this gene produces a single transcript of 2,262 nucleotides, excepting the poly A tail, that presents an unusually long 5' untranslated region (5' UTR) of 223 nucleotides . The C4BPa gene is organized as follows : the first exon codes for the first 198 nuc ...
Response of Jujube Fruits to Exogenous Oxalic Acid Treatment
... (1998) observed that OA treatment induced the activity of chitinase in cucumber leaves. In our previous studies, we reported that exogenous OA treatment could serve as an anti-browning agent for litchi fruit to prevent the pericarp from browning (Zheng and Tian 2006). Treatment with OA at 5 mM slowe ...
... (1998) observed that OA treatment induced the activity of chitinase in cucumber leaves. In our previous studies, we reported that exogenous OA treatment could serve as an anti-browning agent for litchi fruit to prevent the pericarp from browning (Zheng and Tian 2006). Treatment with OA at 5 mM slowe ...
Last update: 06/22/2015
... BLAST stands for Basic Local Alignment Search Tool and is a program that reports regions of similarity (at the nucleotide or protein level) between a query (your input) sequence and sequences within a database. BLAST uses a robust statistical framework that determines if the alignment between two se ...
... BLAST stands for Basic Local Alignment Search Tool and is a program that reports regions of similarity (at the nucleotide or protein level) between a query (your input) sequence and sequences within a database. BLAST uses a robust statistical framework that determines if the alignment between two se ...
Proton n.m.r, spectroscopic evidence for sulfur
... Sulfur-aromatic interactions in position 6 with tyrosine may be found in the chromatographic behavior of these derlvatives (9). Greatly reduced reversed-phase HPLC retention times are observed for derivatives of deamino- and dcamino-carba-l-O'T as compared with deamino-carba-6-0T and deamino-dicarb ...
... Sulfur-aromatic interactions in position 6 with tyrosine may be found in the chromatographic behavior of these derlvatives (9). Greatly reduced reversed-phase HPLC retention times are observed for derivatives of deamino- and dcamino-carba-l-O'T as compared with deamino-carba-6-0T and deamino-dicarb ...
Last update: 06/22/2015 Page 1 of 7 Introduction to BLAST using
... BLAST stands for Basic Local Alignment Search Tool and is a program that reports regions of similarity (at the nucleotide or protein level) between a query (your input) sequence and sequences within a database. BLAST uses a robust statistical framework that determines if the alignment between two se ...
... BLAST stands for Basic Local Alignment Search Tool and is a program that reports regions of similarity (at the nucleotide or protein level) between a query (your input) sequence and sequences within a database. BLAST uses a robust statistical framework that determines if the alignment between two se ...
Project Manual Bio3055 Metabolic Disease: Hypoxanthine
... protein source and any molecules bound to the protein. ...
... protein source and any molecules bound to the protein. ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.