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(mmg) operon of Bacillus
(mmg) operon of Bacillus

... shaped bacterium. It secretes enzymes that are of commercial importance in various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohyd ...
Soy Protein Based Green Composite: A Review
Soy Protein Based Green Composite: A Review

... causing the protein to precipitate out of aqueous solution [14]. It has been found that the soy protein could not form a film at or near its isoelectric point (pH 4.5) due to the coagulation. However, proteins can be denatured and unfolded at pH values away from their isoelectric point, thus exposin ...
H +
H +

... transported and carries it to a membrane-spanning transport protein. Meanwhile, an ATP-hydrolyzing protein breaks ATP down into ADP, phosphate, and energy. It is this energy that powers the transport of the substrate, by way of the membrane-binding transporter, across the membrane and into the cytop ...
SnRK1 Isoforms AKIN10 and AKIN11 Are
SnRK1 Isoforms AKIN10 and AKIN11 Are

... showed that these subunits were not affected by phosphate starvation. Transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the AKIN10 and AKIN11 catalytic subunits fused with green fluorescent protein (GFP) were produced, and their localizations were mainly chloroplastic with low but ...
PAGES 1-41 INCL. 1. Overview (a) discovery of enzymes (b
PAGES 1-41 INCL. 1. Overview (a) discovery of enzymes (b

... - specificity relates to binding of substrate to enzyme - group specific enzymes can use a variety of substrates, each containing a certain functional group which is modified - absolute specificity utilize only one substrate (or specific pair) in one reaction 2.(a) What is a catalyst? - any molecule ...
3. G-protein-coupled receptors
3. G-protein-coupled receptors

... 3. G-protein-coupled receptors (7-TM receptors) 3.4 Bacteriorhodopsin & rhodopsin family • Rhodopsin = visual receptor • Many common receptors belong to this same family • Implications for drug selectivity depending on similarity (evolution) • Membrane bound receptors difficult to crystallise • X-R ...
Facing extremes: archaeal surface-layer (glyco)proteins
Facing extremes: archaeal surface-layer (glyco)proteins

... chains, with sulfated glucuronic acid moieties attached to asparagine-linked glucose residues predominating and a single chain of a sulfated repeating unit pentasaccharide linked through N-acetylgalactosamine positioned at the 2-asparagine position of the protein (Lechner & Wieland, 1989). It remain ...
Jacob Corn
Jacob Corn

Cloning and characterization of CmGPD1, the Candida magnoliae
Cloning and characterization of CmGPD1, the Candida magnoliae

... phosphatase (GPP). Glycerol is a key metabolic intermediate in the carbon flow between glycolytic catabolism and the synthesis of fatty acids in prokaryotes and eukaryotes (Rognstad et al., 1974). Glycerol metabolism is important in biotechnology for ethanol production or wine smoothness (Remize et ...
03_seq_alignemnt_DP
03_seq_alignemnt_DP

... BLOSUM (BLOck SUM) – there are evidence it outperform PAM • Block  proteins in the same family can be aligned without introducing a gap (not the individual seqs.) • So any given protein can contain one or more blocks, corresponding to each of its functional or structural motif • With these protein ...
Worksheet 2
Worksheet 2

... Move your cursor over the bars, what details about the domains are revealed? ________________________________________________________________________ ________________________________________________________________________ ...
IMGT/Collier-de-Perles: a two-dimensional visualization tool for
IMGT/Collier-de-Perles: a two-dimensional visualization tool for

... The IMGT/Collier-de-Perles [14] tool was created by LIGM (Université Montpellier 2, CNRS) and is part of IMGTW, the international ImMunoGeneTics information systemW [15,16] (IMGTW, http://www.imgt.org), which is acknowledged as the global reference in immunogenetics and immunoinformatics. IMGT/Colli ...
perspective - Pseudomonas syringae Genome Resources Home Page
perspective - Pseudomonas syringae Genome Resources Home Page

... Like insects and vertebrates, plants have Figure 1 Extracellular and intracellular PRRs in plants and animals. Animal TLRs and plant receptorreceptors for microbe-associated molecules like kinases are similar in overall structure in that all are transmembrane receptors that have and respond to many ...
Immersive Projection for Biochemical Pattern Matching
Immersive Projection for Biochemical Pattern Matching

... turn stabilises to form the secondary structure (alpha helixes or beta sheets). This stabilised structure then folds to form the tertiary structure, the fully folded, stable state of the protein. The interactions involved in stabilising this folded state of the protein primarily consist of weak van ...
tr-kit promotes the formation of a multimolecular complex composed
tr-kit promotes the formation of a multimolecular complex composed

... carboxyl-terminal domain and it is the main substrate for Src-like kinases in mitosis (Taylor and Shalloway, 1994); it has been demonstrated that the interaction with the SH3 domain of Fyn is necessary for Fyn-dependent tyrosine phosphorylation of Sam68 (Shen et al., 1999). Once phosphorylated in th ...
Protein synthesis meets ABC ATPases: new roles for Rli1/ABCE1
Protein synthesis meets ABC ATPases: new roles for Rli1/ABCE1

... ATPase domain, the Fe–S cluster is required for the function of Rli1/ABCE1 in termination and recycling (Khoshnevis et al, 2010). One might speculate that NTP hydrolysis is coupled to splitting the ribosome into subunits, in analogy to the prokaryotic recycling factors RRF/EF-G that couple the free ...
3. G-protein-coupled receptors
3. G-protein-coupled receptors

... 3. G-protein-coupled receptors (7-TM receptors) 3.4 Bacteriorhodopsin & rhodopsin family • Rhodopsin = visual receptor • Many common receptors belong to this same family • Implications for drug selectivity depending on similarity (evolution) • Membrane bound receptors difficult to crystallise • X-R ...
Reducing Rice Seed Storage Protein Accumulation Leads to
Reducing Rice Seed Storage Protein Accumulation Leads to

... visible on a stained gel (Fig. 1A and Supplemental Fig. S1). On the other hand, levels of Glb-1 and 13kD prolamins significantly increased (Fig. 1A). GluB-less seeds had decreased levels of GluB-4, GluB-2 and GluB-1, whereas levels of Glb-1 and 13kD prolamins increased appreciably (Fig. 1A and Supp ...
Amino Acids And Protein Ppt - GCG-42
Amino Acids And Protein Ppt - GCG-42

... and acidic groups and so can act as base or acid. Neutral amino acids (monobasic, monocarboxylic) exist in aqueous solution as “ Zwitter ion” i.e. contain both positive and negative charge. Zwitter ion is electrically neutral and can’t migrate into electric ...
Defensin-like antifungal proteins secreted by filamentous fungi
Defensin-like antifungal proteins secreted by filamentous fungi

... The transcription of afp is under control of different transcription factors. It is hypothesised that a calcineurin signalling pathway, the phosphate starvation, and an asexual developmental regulation mechanism play a central role in transcription of afp. Five putative Crz1p (stress response elemen ...
PROTEOGLYCANS AND GLYCOPROTEINS
PROTEOGLYCANS AND GLYCOPROTEINS

... The units in the saccharide chains are elongated in alternating acidic/amino sugars, donated from UDP derivatives. Last step is sulfation of some amino sugars. For glycosaminoglycan synthesis and synthesis of O-linked glycoproteins, the addition is direct. For N-linked glycoproteins, the chain is fo ...
Amino Acids And Protein Ppt
Amino Acids And Protein Ppt

... and acidic groups and so can act as base or acid. Neutral amino acids (monobasic, monocarboxylic) exist in aqueous solution as “ Zwitter ion” i.e. contain both positive and negative charge. Zwitter ion is electrically neutral and can’t migrate into electric ...
Basic Local Alignment Search Tool (BLAST) IMBB 19, May 2015
Basic Local Alignment Search Tool (BLAST) IMBB 19, May 2015

... ü  StaCsCcal  measures  like  E-­‐value.  P-­‐Value    and  bit  score   ü  Percentage  idenCty  (%  of  idenCcal  residues  between  sequences)   ü  The  length  of  sequence  stretch  that  is  similar   2.  Homology;  homologs  diverse  f ...
She2p Is a Novel RNA Binding Protein
She2p Is a Novel RNA Binding Protein

... Ash1p is achieved by active localization of ASH1 mRNA to the bud tip of the daughter cell (Long et al., 1997; Takizawa et al., 1997). At the bud tip, the mRNA is translated into Ash1p, which represses transcription of the inducer of mating-type switch (i.e., the HO endonuclease; Bobola et al., 1996; ...
Tris buffered saline (TBS)
Tris buffered saline (TBS)

... ...
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Protein–protein interaction



Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.
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