Meal ingestion, amino acids and brain
... Carbohydrate ingestion raises tryptophan uptake and serotonin synthesis in rat brain. The addition of protein is generally believed only to block such increases. However, some recent evidence suggests dietary protein may not be limited to this action. In the present studies, we fed rats single meals ...
... Carbohydrate ingestion raises tryptophan uptake and serotonin synthesis in rat brain. The addition of protein is generally believed only to block such increases. However, some recent evidence suggests dietary protein may not be limited to this action. In the present studies, we fed rats single meals ...
TFE3 contains two activation domains, one acidic and the other
... (containing both activation domains), TFE3S (containing only the C-terminal activation domain) and TFE3L-ACterm (a truncated protein containing only the N-terminal activation domain). Plasmids encoding these proteins were cotransfected with a reporter dependent upon eight tandem (iE3 sites. Figure 2 ...
... (containing both activation domains), TFE3S (containing only the C-terminal activation domain) and TFE3L-ACterm (a truncated protein containing only the N-terminal activation domain). Plasmids encoding these proteins were cotransfected with a reporter dependent upon eight tandem (iE3 sites. Figure 2 ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
... 2OG oxygenase, YcfD, and the novel microbial carbon-phosphorus (C-P) bond cleavage enzyme, PhnZ, are presented. In each case the identification of a metal ion cofactor and structure determination led to important functional insights. EcSI is encoded by a gene that is highly conserved among pathogeni ...
... 2OG oxygenase, YcfD, and the novel microbial carbon-phosphorus (C-P) bond cleavage enzyme, PhnZ, are presented. In each case the identification of a metal ion cofactor and structure determination led to important functional insights. EcSI is encoded by a gene that is highly conserved among pathogeni ...
Protein degradation in mitochondria
... role has been demonstrated for structurally related ATPase domains of other ATP-dependent proteases.33 This activity, however, is apparently not sufficient to prevent the aggregation of substrate polypeptides, a prerequisite for their degradation by Lon-like proteases. This is achieved by the mitoch ...
... role has been demonstrated for structurally related ATPase domains of other ATP-dependent proteases.33 This activity, however, is apparently not sufficient to prevent the aggregation of substrate polypeptides, a prerequisite for their degradation by Lon-like proteases. This is achieved by the mitoch ...
Isotope-labeled Peptides
... frequencies of covalent bonds, so it allows conformational studies by infrared spectroscopy. Nuclear Magnetic Resonance (NMR) is a powerful technique for determining the structures, dynamics, and molecular interactions of biomolecules. As more and more peptides advance in clinical trials, both as th ...
... frequencies of covalent bonds, so it allows conformational studies by infrared spectroscopy. Nuclear Magnetic Resonance (NMR) is a powerful technique for determining the structures, dynamics, and molecular interactions of biomolecules. As more and more peptides advance in clinical trials, both as th ...
TAFs and the Mediator
... More than half (9 out of 14) of the yTAFIIs contain a histone fold motif, and they specifically assemble into five histone-like pairs The histone fold is the fundamental interaction motif involved in heterodimerization of the core histones, H4 and H3, and H2A and H2B. ...
... More than half (9 out of 14) of the yTAFIIs contain a histone fold motif, and they specifically assemble into five histone-like pairs The histone fold is the fundamental interaction motif involved in heterodimerization of the core histones, H4 and H3, and H2A and H2B. ...
Enzymatic properties of the N- and C
... N-terminal and Δ18N constructs show higher affinity for ATP than the C-terminal half. Many lines of evidence suggest that GST or the GST-domains of fusion proteins have the potential to mediate oligomerization both in vitro and in vivo (23, 24). In particular, Tudyka and Skerra mentioned that when c ...
... N-terminal and Δ18N constructs show higher affinity for ATP than the C-terminal half. Many lines of evidence suggest that GST or the GST-domains of fusion proteins have the potential to mediate oligomerization both in vitro and in vivo (23, 24). In particular, Tudyka and Skerra mentioned that when c ...
Triphosphatase Related to the Protein Tyrosine Phosphatases
... that also hydrolyze phosphoserine and phosphothreonine to some extent (reviewed in Denu et al., 1996; Fauman and Saper, 1996). PTPs have been implicated in several processes, including signal transduction and cell cycle control. CEL-1 represents the first member of this family to recognize an RNA su ...
... that also hydrolyze phosphoserine and phosphothreonine to some extent (reviewed in Denu et al., 1996; Fauman and Saper, 1996). PTPs have been implicated in several processes, including signal transduction and cell cycle control. CEL-1 represents the first member of this family to recognize an RNA su ...
Unit 2 Objectives - Chemistry of Life
... 1.1 Recognize that biological organisms are composed primarily of very few elements. The six most common are C, H, N, O, P, and S. 1.2 Describe the basic molecular structures and primary functions of the four major categories of organic molecules (carbohydrates, lipids, proteins, nucleic acids). 1.3 ...
... 1.1 Recognize that biological organisms are composed primarily of very few elements. The six most common are C, H, N, O, P, and S. 1.2 Describe the basic molecular structures and primary functions of the four major categories of organic molecules (carbohydrates, lipids, proteins, nucleic acids). 1.3 ...
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus
... Purified recombinant sortase lacking its N-terminal signal peptide/membrane anchor catalyzes the transpeptidation reaction of surface protein anchoring in vitro, using LPXTG peptides and NH2-Gly3 as a peptidoglycan substrate (9). Both the in vivo and in vitro reactions of sortase can be inhibited wi ...
... Purified recombinant sortase lacking its N-terminal signal peptide/membrane anchor catalyzes the transpeptidation reaction of surface protein anchoring in vitro, using LPXTG peptides and NH2-Gly3 as a peptidoglycan substrate (9). Both the in vivo and in vitro reactions of sortase can be inhibited wi ...
Document
... Example of conserved regions (PPID family) - 1 CSA_PPIASE (cyclophilin-type peptydil-prolyl cis-trans isomerase) (domain) - 3 TPR repeats (tetratrico peptide repeat). - 1 active site ...
... Example of conserved regions (PPID family) - 1 CSA_PPIASE (cyclophilin-type peptydil-prolyl cis-trans isomerase) (domain) - 3 TPR repeats (tetratrico peptide repeat). - 1 active site ...
Crystal structure of the S187F variant of human liver alanine
... The three-dimensional coordinates of the S187F mutant in the internal aldimine form were used as a starting point to generate the external aldimine and PMP bound forms of the enzyme, by means of energy minimization. These enzymatic forms of normal AGT and variant were generated using the BIOPOLYMER ...
... The three-dimensional coordinates of the S187F mutant in the internal aldimine form were used as a starting point to generate the external aldimine and PMP bound forms of the enzyme, by means of energy minimization. These enzymatic forms of normal AGT and variant were generated using the BIOPOLYMER ...
MODified™ Protein Domain Binding Kit Manual
... residues, phosphorylation of serine or threonine residues, citrullination and ubiquitination. These epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ ...
... residues, phosphorylation of serine or threonine residues, citrullination and ubiquitination. These epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ ...
Selected Reaction Monitoring (SRM) to determine protein
... single peptide fragment ion is selected in Q3 for quantitation. The precursor and fragment ion pair is referred to as a transition and its abundance is used to quantify the abundance of a specific peptide derived from a protein. During an SRM experiment, sequential gating of precursor and product in ...
... single peptide fragment ion is selected in Q3 for quantitation. The precursor and fragment ion pair is referred to as a transition and its abundance is used to quantify the abundance of a specific peptide derived from a protein. During an SRM experiment, sequential gating of precursor and product in ...
4 - EMD Millipore
... This guide is a resource for scientists for the preparation and use of research essential biochemicals, such as antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. You will find this guide to be a useful resource, whether ...
... This guide is a resource for scientists for the preparation and use of research essential biochemicals, such as antibiotics, buffers, detergents, dyes, stains, and substrates, which are indispensable for any life science research laboratory. You will find this guide to be a useful resource, whether ...
Citrate synthase proteins in extremophilic organisms: Studies within
... The primary characteristics of globular proteins are their native structures and sequences of amino acids. An evolutionary divergence from a common ancestor may lead diverse sequences to fold to nearly the same native structure. One particularly interesting example is citrate synthase (CS), an enzym ...
... The primary characteristics of globular proteins are their native structures and sequences of amino acids. An evolutionary divergence from a common ancestor may lead diverse sequences to fold to nearly the same native structure. One particularly interesting example is citrate synthase (CS), an enzym ...
МИНИСТЕРСТВО ОБРАЗОВАНИЯ И НАУКИ
... know: -classification, structure, biological role and metabolic pathways of main biomolecules included in the animal and plant cells, methods for storing and transmitting genetic information, the principles of energy transformation in biological systems, the main methods for the synthesis of biologi ...
... know: -classification, structure, biological role and metabolic pathways of main biomolecules included in the animal and plant cells, methods for storing and transmitting genetic information, the principles of energy transformation in biological systems, the main methods for the synthesis of biologi ...
P6060Datasheet-Lot0151208
... Mix the substrate with 1X NEBuffer for PK supplemented with ATP. Add the activated CaMKII. Incubate at 30°C. ...
... Mix the substrate with 1X NEBuffer for PK supplemented with ATP. Add the activated CaMKII. Incubate at 30°C. ...
Structural Insights into Triglyceride Storage Mediated by
... evolutionarily conserved proteins localized to the endoplasmic reticulum that are involved in triglyceride lipid droplet formation. FIT proteins have been shown to mediate the partitioning of cellular triglyceride into lipid droplets, but not triglyceride biosynthesis. FIT proteins do not share prim ...
... evolutionarily conserved proteins localized to the endoplasmic reticulum that are involved in triglyceride lipid droplet formation. FIT proteins have been shown to mediate the partitioning of cellular triglyceride into lipid droplets, but not triglyceride biosynthesis. FIT proteins do not share prim ...
iirachhemoglobin
... Normally, an individual has 4 genes that code for the α protein, or α chain. Two other genes code for the β chain. The α chain and the β chain are made in precisely equal amounts, despite the differing number of genes. The protein chains join in developing red blood cells, and remain together for th ...
... Normally, an individual has 4 genes that code for the α protein, or α chain. Two other genes code for the β chain. The α chain and the β chain are made in precisely equal amounts, despite the differing number of genes. The protein chains join in developing red blood cells, and remain together for th ...
The use of deuteration for the structural study of larger proteins
... • 13C or 12C labeling at protonated positions (reverse labeling) (Vuister et al. J. Am. Chem. Soc. 1994, 116, 9206) e.g. attractive for aromatic residues ...
... • 13C or 12C labeling at protonated positions (reverse labeling) (Vuister et al. J. Am. Chem. Soc. 1994, 116, 9206) e.g. attractive for aromatic residues ...
Aspects of Reductive Explanation in Biological Science: Intrinsicality
... Structure of Science ([1961]). Schaffner argued that reduction was occurring in biology, especially in the molecularization of genetics ([1969]), but that this philosophical interpretation was not relevant to ongoing research methodology ([1974]). The ensuing discussion about the relationship betwee ...
... Structure of Science ([1961]). Schaffner argued that reduction was occurring in biology, especially in the molecularization of genetics ([1969]), but that this philosophical interpretation was not relevant to ongoing research methodology ([1974]). The ensuing discussion about the relationship betwee ...
PyMOL Modelling Workshop Outline of PyMOL usage
... When assessing a structure, one needs to take account of the resolution and R-factor, error estimates and missing residues. There is information about the sequence that was used to determine the structure with a sequence database reference. There is also information about the biological unit. In the ...
... When assessing a structure, one needs to take account of the resolution and R-factor, error estimates and missing residues. There is information about the sequence that was used to determine the structure with a sequence database reference. There is also information about the biological unit. In the ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.