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valve margin
valve margin

... which is required for leaf outgrowth. ...
SPATULA, a bHLH carpel development gene
SPATULA, a bHLH carpel development gene

... cDNA 3.5 corresponds to predicted gene 44 in the AP2 contig of Terryn et al. (1999) (also called AT4g36930 by the Martinsried Institute for Protein Sequences, see http://www.mips.biochem.mpg.de/proj/thal/). Comparison of cDNA 3.5 and the predicted ORF suggested that it is not full length. Two longer ...
The evolutionary history of human chromosome 7
The evolutionary history of human chromosome 7

... been investigated only by comparative banding analysis. To delineate the subchromosomal organization of human chromosome 7 homologs in nonhuman primates and to reconstruct the sequence of evolutionary intrachromosomal rearrangements in greater detail, we used a series of subregional DNA probes for c ...
Sleeping Beauty - Weber State University
Sleeping Beauty - Weber State University

... its original location and promotes its reintegration elsewhere in the genome (Plasterk, 1996). Autonomous members of a transposon family can express an active transposase, the trans-acting factor for transposition, and thus are capable of transposing on their own. Nonautonomous elements have mutated ...
8p interstitial deletions including 8p12 FTNW
8p interstitial deletions including 8p12 FTNW

... the broken ends have fused, leaving out the deleted segment. The size of the missing piece varies between different individuals. For healthy development, chromosomes should contain just the right amount of genetic material – not too much and not too little. An interstitial deletion 8p including 8p12 ...
Runx1 modulates developmental, but not injury
Runx1 modulates developmental, but not injury

... repression. As Runx1 was known to be a master regulator of blood stem cells (Speck and Gilliland, 2002; Speck et al., 2002), we hypothesized that it might also play a regulatory role in HFSCs. To begin to examine its role in HFSCs we first determined Runx1 expression patterns in skin development in ...
Causes, Risk Factors, and Prevention Colorectal Cancer Risk Factors
Causes, Risk Factors, and Prevention Colorectal Cancer Risk Factors

... Some gene mutations happen during a person’s lifetime and are not passed on. They affect only cells that come from the original mutated cell. These DNA changes are due to acquired mutations. In most cases of colorectal cancer, the DNA mutations that lead to cancer are acquired during a person’s life ...
Document
Document

... types are involved and whether CRFs act through cell expansion or control of cell division or both. One approach to achieving this is through a detailed tissue and cellular level examination of the leaves and cotyledons of CRF mutants during development. In a parallel approach I will make use of tra ...
Massive Changes in Genome Architecture Accompany
Massive Changes in Genome Architecture Accompany

... Present address: Fox Chase Cancer Center, Philadelphia, PA 19111. ...
M2 RNA Pol Ⅰ genes
M2 RNA Pol Ⅰ genes

... C selectivity factor SLl stabilizes the UBF-DNA complex. D SL1 contains several subunits including the TATA-binding protein TBP. E in Acanthamoeba there is a single control element in rRNA gene promoters. 4. Which two of the following statements about RNA Pol III genes are true? A the transcriptiona ...
Characterisation of the katA gene encoding a catalase and evidence
Characterisation of the katA gene encoding a catalase and evidence

... A KatA-de¢cient strain of S. xylosus, designated TX350, was constructed by exchanging the 5P-end of katA for an erythromycin resistance gene, ermB (Fig. 1B). By this replacement, the ¢rst 60 codons of katA including the start codon and ribosome-binding site were removed resulting in a complete block ...
Canadian journal of Microbiology
Canadian journal of Microbiology

... S. sclerotiorum in commercially grown canola cultivars, management of this pathogen is essential. Public concern over the use of chemical pesticides together with the potential for acquiring resistance to these compounds has led to renewed interest in bacterial antagonists like PA23 for biocontrol. ...
Agrobacterium: nature`s genetic engineer
Agrobacterium: nature`s genetic engineer

... Since the biological functions encoded by these genes were not yet known, the full significance of these data could not be appreciated. However, the use of promoters encoded in the T-DNA proved invaluable in the genetic engineering of plants. Once a restriction map of an octopine Ti plasmid was gene ...
Unit 19 Handout - Chavis Biology
Unit 19 Handout - Chavis Biology

Large-Scale Chromosomal Changes
Large-Scale Chromosomal Changes

... c. Cytologically, inversions can be detected by banding, and when heterozygous, they show the typical twisted “inversion” loop during homologous pairing. Pericentric inversions can result in a change in the p:q ratio (the position of the centromere). Genetically, no viable crossover products are see ...
Chromosomal translocations deregulated BCL6
Chromosomal translocations deregulated BCL6

... PLZF (Chen et al., 1993) proteins. The BCL6 gene is tightly regulated during B cell differentiation, being expressed in mature B cells within germinal centers but not in immature B cell precursors or differentiated plasma cells (Ye et al., 1994; Cattoretti et al., 1995; Flenghi et al., 1995). Its fe ...
Construction and Characterization of a Highly Regulable Expression
Construction and Characterization of a Highly Regulable Expression

... A number of different expression vectors have been developed to facilitate the regulated overproduction of proteins in Escherichia coli and related bacteria. Some of the more popular ones include pKK223-3, pKK233-2, pTrc99A, and the pET family of expression vectors. These vectors were designed to be ...
IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll
IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll

... and Bourgeois, supra), and is itself rapidly converted to chromosome to improve control of heterologous gene glucose and galactose. expression from a lac operated promoter (as long as a Another control element of the lac operon is catabo promoter is present to drive lacIZYA transcription). It lite r ...
Interactions Between Genes Controlling Pathogenicity in the Flax
Interactions Between Genes Controlling Pathogenicity in the Flax

... identified. These occur in five series of closely linked or allelic genes, designated K, L, M, N, and P, which contain 1, 13, 7, 3, and 5 genes, respectively (6,11,19,20). In studies of the inheritance of pathogenicity in flax rust, single-gene segregation ratios indicating a dominance of avirulence ...
Pitx1 and Pitx2 are required for development of hindlimb buds
Pitx1 and Pitx2 are required for development of hindlimb buds

... in Pitx1–/– embryos (Lanctôt et al., 1999b; Szeto et al., 1999) and which is similarly decreased in double mutant embryos (Fig. 4A). Thus, HL bud size reduction affects both outgrowth and width of the bud along the AP axis. Bud outgrowth is thought to be controlled by growth factors produced by the ...
Transgenic approaches for optogenetics Tim Murphy UBC.
Transgenic approaches for optogenetics Tim Murphy UBC.

... Cre is often expressed from a randomly integrated transgene, but very few insertion sites are known. Random transgene integration can disrupt an endogenous mouse gene and cause unanticipated side effects. Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygou ...
Robust gene silencing mediated by antisense small RNAs in the
Robust gene silencing mediated by antisense small RNAs in the

... shown. For all experiments, the negative control has no luciferase expression. (A) Schematic of constructs used in this assay. The trigger was fused upstream of the luciferase-coding region and driven by either the endogenous promoter or the CS promoter. (B) The 132-bp trigger of EHI_197520 results ...
Cloning and expression of the phosphotriesterase
Cloning and expression of the phosphotriesterase

... enzymic activity in library clones. In a previous study, an OP-degrading gene was successfully isolated from an Agrobacterium strain by mobilizing a genomic library into a related strain lacking activity (Horne et al., 2002a). A similar approach was taken here, using Pseudomonas aeruginosa BENNY, a ...
Development 130, 3567-3578. - Howard Hughes Medical Institute
Development 130, 3567-3578. - Howard Hughes Medical Institute

... allantois (Fig. 2D,D′) were observed. The rest of the mutants (46) were morphologically indistinguishable from their heterozygous and wild-type littermates. The phenotype of the four abnormal mutants was similar to, but less pronounced than, the ventralization of the mesoderm observed in double homo ...
Imprinting in the endosperm: a possible role in preventing wide
Imprinting in the endosperm: a possible role in preventing wide

... failure in reproductive isolation and angiosperm speciation. The genetic basis of endosperm failure remains unclear, although gene dosage effects (Birchler 1993) and imprinting of regulatory genes (Haig & Westoby 1991) have been proposed as contributory factors. Imprinting is a mitotically stable ep ...
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Polycomb Group Proteins and Cancer

The Polycomb-group proteins (PcGs) are a family of proteins that use epigenetic mechanisms to maintain or repress expression of their target genes. They were originally discovered in Drosophila (fruit flies), though they've been shown to be conserved in many species due to their vital roles in embryonic development. These proteins' ability to alter gene expression has made them targets of investigation for research groups seeking to understand disease pathology and oncology.
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