Mutations affecting craniofacial development in zebrafish
... of Kelly and Bryden (1983). Larvae at 5 dpf or older were fixed overnight in 4% phosphate-buffered paraformaldehyde and washed several times in phosphate-buffered saline with 0.1% Tween-20 (PBT). In order to enhance their optical clarity, the specimens were bleached in 30% hydrogen peroxide for 2 ho ...
... of Kelly and Bryden (1983). Larvae at 5 dpf or older were fixed overnight in 4% phosphate-buffered paraformaldehyde and washed several times in phosphate-buffered saline with 0.1% Tween-20 (PBT). In order to enhance their optical clarity, the specimens were bleached in 30% hydrogen peroxide for 2 ho ...
What Size Are Your Genes?
... This means that the smaller the molecule, the faster it migrates through the gel. Molecules can have the same molecular weight and charge but different shapes. Molecules having a more compact shape (a sphere is more compact than a rod) can move more easily through the pores. In this experiment, dyes ...
... This means that the smaller the molecule, the faster it migrates through the gel. Molecules can have the same molecular weight and charge but different shapes. Molecules having a more compact shape (a sphere is more compact than a rod) can move more easily through the pores. In this experiment, dyes ...
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1
... 1999), intestine (Mitchelmore et al., 2000) and kidney (Pontoglio et al., 2000). Although mutations in human hnf1a gene have been shown to cause maturity-onset diabetes of the young, i.e., MODY3 (Yamagata et al., 1996), hnf1a does not seem to play an essential role during development (Pontoglio et a ...
... 1999), intestine (Mitchelmore et al., 2000) and kidney (Pontoglio et al., 2000). Although mutations in human hnf1a gene have been shown to cause maturity-onset diabetes of the young, i.e., MODY3 (Yamagata et al., 1996), hnf1a does not seem to play an essential role during development (Pontoglio et a ...
PAX6 mRNA Transcript Analysis in Various Ocular/Non
... commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expression profiles for the maintenance/housekeeping genes exhibit similar patterns for each specific tissue type (Figure 1A). As a ...
... commonly considered to have a housekeeping function (e.g., β-actin and GAPDH) exhibit considerably variable expression levels from one tissue type to another. However, expression profiles for the maintenance/housekeeping genes exhibit similar patterns for each specific tissue type (Figure 1A). As a ...
Chapter 29 Slides
... Genetic Information Can Be Transferred Between Bacteria • In 1946, Lederberg and Tatum showed that two different strains of bacteria with different growth requirements could exchange genes • Lederberg and Tatum surmised that the bacterial cells must interact with each other - the process is now know ...
... Genetic Information Can Be Transferred Between Bacteria • In 1946, Lederberg and Tatum showed that two different strains of bacteria with different growth requirements could exchange genes • Lederberg and Tatum surmised that the bacterial cells must interact with each other - the process is now know ...
Homo-Dimeric Spherulin 3a: A Single
... with a wide variety of different functions. Common features of these proteins are 1. the Greek-key motif of antiparallel -sheets, called the crystallin fold, and 2. the high intrinsic long-term stability. Spherulin 3a (S3a), a dormant protein from the spherules of Physarum polycephalum, is the only ...
... with a wide variety of different functions. Common features of these proteins are 1. the Greek-key motif of antiparallel -sheets, called the crystallin fold, and 2. the high intrinsic long-term stability. Spherulin 3a (S3a), a dormant protein from the spherules of Physarum polycephalum, is the only ...
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and
... 2006), and the non-teleostean gar and bowfin (Amemiya and Youson 2004). All these hormones share consistent structural features, including a conserved N-glycosylation site and 10 invariant cysteine residues that form intrachain disulfide bonds. Also, an 11th conserved cysteine residue that is critic ...
... 2006), and the non-teleostean gar and bowfin (Amemiya and Youson 2004). All these hormones share consistent structural features, including a conserved N-glycosylation site and 10 invariant cysteine residues that form intrachain disulfide bonds. Also, an 11th conserved cysteine residue that is critic ...
Novel p53 mutants selected in BRCA
... in the aetiology of BRCA-associated breast cancer. Inheritance of mutant alleles of the breast cancer susceptibility genes BRCA1 and BRCA2 is associated with a substantial risk of developing breast, ovarian and some other cancers (Miki et al., 1994; Wooster et al., 1996). Tumorigenesis in carriers o ...
... in the aetiology of BRCA-associated breast cancer. Inheritance of mutant alleles of the breast cancer susceptibility genes BRCA1 and BRCA2 is associated with a substantial risk of developing breast, ovarian and some other cancers (Miki et al., 1994; Wooster et al., 1996). Tumorigenesis in carriers o ...
by Attila Mokanszki Supervisor: Prof. Dr. Eva Olah
... spinal and bulbular muscular athrophy (SBMA). The expansion of poliglutamin repeat relates to male infertility and insufficient masculinisation (MAIS). Cystic fibrosis is one of the most common autosomal recessive disease, affecting one in 2500 live births. Insufficient functioning of the CFTR prot ...
... spinal and bulbular muscular athrophy (SBMA). The expansion of poliglutamin repeat relates to male infertility and insufficient masculinisation (MAIS). Cystic fibrosis is one of the most common autosomal recessive disease, affecting one in 2500 live births. Insufficient functioning of the CFTR prot ...
Bcl-2–Modifying Factor Induces Renal Proximal Tubular
... long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by -nc-nd/3.0/ for details. ...
... long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by -nc-nd/3.0/ for details. ...
Translational control of regA, a key gene controlling
... Kochert, 1982; Pommerville and Kochert, 1981; Schmitt, 2003). Three types of genes – gls, lag and regA – are responsible for cell differentiation in V. carteri. The regA gene is a master control gene that is expressed only in somatic cells, where it suppresses all reproductive activity. If the regA ...
... Kochert, 1982; Pommerville and Kochert, 1981; Schmitt, 2003). Three types of genes – gls, lag and regA – are responsible for cell differentiation in V. carteri. The regA gene is a master control gene that is expressed only in somatic cells, where it suppresses all reproductive activity. If the regA ...
Embryo forming cells in carrot suspension cultures
... antibodies do not increase the number of somatic embryos developing in embryogénie cell cultures. An AGP fraction purified with the JIM8 antibody even decreased the number of somatic embryos. Low-embryogenic carrot suspension cultures treated with carrot-seed AGPs did show an increased frequency of ...
... antibodies do not increase the number of somatic embryos developing in embryogénie cell cultures. An AGP fraction purified with the JIM8 antibody even decreased the number of somatic embryos. Low-embryogenic carrot suspension cultures treated with carrot-seed AGPs did show an increased frequency of ...
Natural variation in monoterpene synthesis in kiwifruit
... (TF) binding sites in the AaTPS1 promoter, and cloned members of both TF classes were able ...
... (TF) binding sites in the AaTPS1 promoter, and cloned members of both TF classes were able ...
fulltext - DiVA portal
... Salmonella virulence was analyzed using the S. enterica serovars Brandenburg, Indiana, Panama, and Schwarzengrund; 21 different serotypes of the strains were examined and the presence of ClyA was suggested to be associated with virulence in these S. enterica serovars [11]. In bacteria, the process o ...
... Salmonella virulence was analyzed using the S. enterica serovars Brandenburg, Indiana, Panama, and Schwarzengrund; 21 different serotypes of the strains were examined and the presence of ClyA was suggested to be associated with virulence in these S. enterica serovars [11]. In bacteria, the process o ...
Foxf1 controls mesoderm differentiation
... and the exocoelomic fluid. The yolk sac is also the initial site of hematopoiesis and provides the nucleated red blood cells that are the first to colonize the embryonic vasculature. Vasculogenesis and hematopoiesis take place in the mesodermal blood islands, followed by vascular remodeling and spro ...
... and the exocoelomic fluid. The yolk sac is also the initial site of hematopoiesis and provides the nucleated red blood cells that are the first to colonize the embryonic vasculature. Vasculogenesis and hematopoiesis take place in the mesodermal blood islands, followed by vascular remodeling and spro ...
A global picture of tRNA genes in plant genomes
... monocots, respectively. The internal or external motifs required for efficient transcription of tRNA genes by RNA polymerase III are well conserved among angiosperms. A brief analysis of the mitochondrial and plastidial tRNA gene populations is also provided. Keywords: alga, angiosperm, non-coding R ...
... monocots, respectively. The internal or external motifs required for efficient transcription of tRNA genes by RNA polymerase III are well conserved among angiosperms. A brief analysis of the mitochondrial and plastidial tRNA gene populations is also provided. Keywords: alga, angiosperm, non-coding R ...
Supporting Information Parfenov et al. 10.1073/pnas.1416074111
... mean-centered the RPKM values and divided by their SD for each exon. The lengths of the composite exons were transformed using the square root of the length. DNA Methylation and mRNA Expression Analysis. The Infinium HM450k array was used for TCGA Head and Neck samples (13). This platform includes p ...
... mean-centered the RPKM values and divided by their SD for each exon. The lengths of the composite exons were transformed using the square root of the length. DNA Methylation and mRNA Expression Analysis. The Infinium HM450k array was used for TCGA Head and Neck samples (13). This platform includes p ...
PINK1- and Parkin- mediated mitophagy at a glance
... protect cells under stress, are not known to function in mitophagy. Presumably, substrates involved in PINK1-mediated Parkin recruitment would be located either on the OMM or in the cytosol. For Parkin, as suggested above, it has not been fully elucidated how many proteins are subject to ubiquitylat ...
... protect cells under stress, are not known to function in mitophagy. Presumably, substrates involved in PINK1-mediated Parkin recruitment would be located either on the OMM or in the cytosol. For Parkin, as suggested above, it has not been fully elucidated how many proteins are subject to ubiquitylat ...
Control of GL2 expression in Arabidopsis leaves and trichomes
... nonspecific transcriptional activation, two additional GL2 reporter constructs were analyzed in that genetic background A. ...
... nonspecific transcriptional activation, two additional GL2 reporter constructs were analyzed in that genetic background A. ...
Distribution and modulatory roles of neuropeptides
... Neuropeptides and neurotransmitter receptors Signal transduction most commonly occurs through two types of receptors; G-protein coupled receptors (GPCRs) also known as metabotropic receptors and ligand-gated ion channel receptors (LGICs). GPCRs are characterized by seven transmembrane (7TM) protein ...
... Neuropeptides and neurotransmitter receptors Signal transduction most commonly occurs through two types of receptors; G-protein coupled receptors (GPCRs) also known as metabotropic receptors and ligand-gated ion channel receptors (LGICs). GPCRs are characterized by seven transmembrane (7TM) protein ...
The development of apical embryonic pattern in Arabidopsis
... embryo. Thus, cotyledons and leaves differ in the type of cells from which they derive; all of the cells of true leaves originate from cells that express STM, but the bulk of the cotyledons originate from cells that have never expressed STM. The band of STM expression persists through the heart stag ...
... embryo. Thus, cotyledons and leaves differ in the type of cells from which they derive; all of the cells of true leaves originate from cells that express STM, but the bulk of the cotyledons originate from cells that have never expressed STM. The band of STM expression persists through the heart stag ...
Identification of the mRNA targets of tRNA
... stability and translational decoding properties, may be central to regulating particular genes, or groups of genes. Mutations in the yeast TRM9 tRNA modification gene drive altered expression of genes enriched in codons targeted by Trm9-modified tRNAs (39). Similarly, mutations in the eukaryotic Elo ...
... stability and translational decoding properties, may be central to regulating particular genes, or groups of genes. Mutations in the yeast TRM9 tRNA modification gene drive altered expression of genes enriched in codons targeted by Trm9-modified tRNAs (39). Similarly, mutations in the eukaryotic Elo ...
Reproductive barriers in indica-japonica rice
... wall metabolism, seven GO terms involved in stress or stimulus response, and eleven ...
... wall metabolism, seven GO terms involved in stress or stimulus response, and eleven ...