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Notes Chapter 24 Bacteria
Notes Chapter 24 Bacteria

...  Bacteria are prokaryotic single-celled organisms. They occur in several variations of three basic shapes: rods, spheres, and spirals.  Bacteria are the oldest and most populous organisms. They are believed to have existed on Earth for about 3.5 billion years.  Bacteria are classified in two king ...
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... 3.1.2 + 3.1.5 Lab Procedures when Handling Micro-organisms Micro-organisms • Can only be seen with the help of a microscope. • Include bacteria, some fungi (yeast) and some protists (plankton). • Occupy a wide range of habitats, e.g. salt water, fresh water, soil, dust, air, hot springs, etc. • Fung ...
Environmental Evolution
Environmental Evolution

... complex multicellular life forms (Hartman et al., 1987). There is near scientific consensus on the generation of phylogenetic diversity through spontaneous mutation, endosymbiosis, and natural selection, but there is considerably less agreement on the origin of unicellular life forms, and on the pro ...
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IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)
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... species being Staphylococcus aurous (30.25%), The second dominant bacterium was Escherichia coli (26.89%), Other species were isolated at relatively lower rates. The bacterial isolates and their absolute/relative abundance are presented on Table 2 and Fig. 2 On the other hand, the bacterial isolates ...
Isolation of Lipoteichoic Acids from Butyrivibrio
Isolation of Lipoteichoic Acids from Butyrivibrio

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Acetic acid, found in vinegar, shown to be effective against bacteria

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View in PDF - Jimma University

... this classification, all wards that were included in the study were not in hygienic conditions (Table 4). These might be because of the number of individuals in the wards. At the time of this study, all wards were at their maximum capacity, as of visitors in and out the wards, the high density of pa ...
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... Microbiology and immunology belong to basic subjects the knowledge of which is necessary to the doctors, all medical workers as these sciences help to decide important problems of clinical, medico-prophylactic and theoretical medicine. The contemporary facts prove that about 70% of all registered di ...
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... Bacteria play important roles in ecosystems. • Prokaryotes have many functions in ecosystems. – photosynthesize – recycle carbon, nitrogen, hydrogen, sulfur – fix nitrogen = Nitrogen fixation Root nodules of white clover contain Nitrogen fixing bacteria, which convert atmospheric nitrogen into a for ...
Current Microbiology 18:
Current Microbiology 18:

... Antlsera production and IgG purification. Whole cells of A. brasilense Cd were used to elicit antibodies. Cells were harvested from the liquid culture by centrifugation at 12,000 g for 10 min at 4 - I~ and washed three times in sterile potassium phosphatebuffered saline (PBS), pH 7.2, and their numb ...
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... infected rye is milled into flour and consumed. • On the other hand, some toxin extracted from fungi have medicinal uses when administered at weak doses. ...
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... infected rye is milled into flour and consumed. • On the other hand, some toxin extracted from fungi have medicinal uses when administered at weak doses. ...
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... • three main groups: • 1. Methanogens are named for their unique form of energy metabolism. • They use H2 to reduce CO2 to methane (CH4) and are strict anaerobes. • live in marshes and swamps- methane that bubbles out at these sites forms marsh gas • are used in sewage treatment and contribute to th ...
Chapter 6: Microbial Growth
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... atmospheric oxygen must produce an enzyme superoxide dismutase (SOD), to get rid of them. SOD is made by aerobes, facultative anaerobes, and aerotolerant anaerobes, but not by anaerobes or microaerophiles. ...
Funky Fomites and Aseptic Microbiology
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... Clostridium. Other major groups include the Actinobacteria, Planctomycetes, Deinococci, and Thermotogae. Gram Negative Bacteria: Bacteria that are gram-negative are not stained dark blue or violet by Gram staining, in contrast to gram-positive bacteria. The difference lies in the cell wall of the tw ...
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Basic Principle of Microbiology

... pressure outside the cell is so low. - Bacteria have evolved their structures and functions to adapt to these conditions. ...
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12 Selective and Differential Media Part I

... • TSA, Nutrient agar, and Luria-Bertani (LB) are all examples of enrichment media. • They are designed to provide the nutrients needed to support the growth of microbes. • Media can contain selective agents that will result in media that is specific for certain microorganisms. • Selective: The media ...
Microbial Source Tracking • Pathogen Contamination  –What is it?
Microbial Source Tracking • Pathogen Contamination –What is it?

... qPCR in Microbial Source Tracking • 16S rRNA gene found in nearly all bacteria and Archaea • Small changes in genes allow for identification of hosts • qPCR allows for quantification of specific host inputs ...
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Phospholipid-derived fatty acids



Phospholipid-derived fatty acids (PLFA) are widely used in microbial ecology as chemotaxonomic markers of bacteria and other organisms. Phospholipids are the primary lipids composing cellular membranes. Phospholipids can be saponified, which releases the fatty acids contained in their diglyceride tail. Once the phospholipids of an unknown sample are saponified, the composition of the resulting PLFA can be compared to the PLFA of known organisms to determine the identity of the sample organism. PLFA analysis may be combined with other techniques, such as stable isotope probing to determine which microbes are metabolically active in a sample. PLFA analysis was pioneered by D.C. White, MD, PhD, at the University of Tennessee, in the early to mid 1980s.
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