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Simple Stains and Gram Stains
Simple Stains and Gram Stains

... microscopy. This approach allows one to observe bacteria in terms of their motility and provides some insight on the organism’s overall morphology. However, since the bacterial cell is transparent and motile and therefore somewhat difficult to observe when using the compound brightfield microscope, ...
Lecture 6
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... Types of Biological Processes The principle categories of biological processes are: • Suspended growth processes • Attached growth (bio-film) processes ...
ANSWERS TO REVIEW QUESTIONS – CHAPTER 37
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2_Chisto+Gille+Shiel+Tayl+Tlus_Baird2010
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... Use established community to understand “pioneers” Simple laboratory model What type of bacteria are present as the lesion worsens? ...
Archaea
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Identifying Uropathogens
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... Gram negative bacilli are of 2 major types, either lactose fermenting or non-lactose fermenting. To determine the type, different media are used to culture the bacteria and detect lactose fermentation. For example, MacConkey's agar and CLED agar. ...
1 slide/page - University of San Diego Home Pages
1 slide/page - University of San Diego Home Pages

... B. Zygomycota (zygote fungi) •  ~1,000 species •  Terrestrial, soil, decaying plant/animal tissue. •  Mycorrhizae: mutualistic association with plant roots. •  Hyphae coenocytic. •  Zygosporangia are the reproductive structures that give the name. •  E.g. Rhizopus stolonifer, black bread mold. ...
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I. Mycology: Mushrooms, molds, and yeasts. General Characteristics
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FUNGI Five lineages of Fungi (Chytrids paraphyletic) II. Structure
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Production of bacteriocine from soil micro organisms to inhibit
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Chapter 1: The Microbial World and You
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... instantaneously. Fig. 2(b) depicts the bead at intervals of 0.6 seconds for 6 seconds. It is shown that the bead does not move at all. To resume the motion of the bead, 7.5 µl of 5 × 10 −3 M EDTA solution was added to the enclosure. The PS bead resumed its motion immediately. Fig. 2(c) depicts the b ...
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Chapter 6
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Basic Research on Bacteria - American Society for Microbiology
Basic Research on Bacteria - American Society for Microbiology

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Fewidobacterium gondwanense sp. nov., a New Thermophilic
Fewidobacterium gondwanense sp. nov., a New Thermophilic

... G+C content. DNA was isolated and purified by using a slight modification of the method of Marmur (10) in which an RNase treatment step was included after cell lysis. DNA was resuspended in 0.1 X SSC (1 X SSC is 0.15 M NaCl plus 0.015 M sodium citrate), and the thermal denaturation temperature was d ...
Topic J04 Microbes and outer influences, decontamination
Topic J04 Microbes and outer influences, decontamination

... Task 1: Influence of various factors to bacterial growth and survival Task 1a) Influence of temperature to bacteria Observe the results of an experiment. The bacteria in the first row were cultivated at various temperatures. The bacteria in the second row were cultivated at the same temperatures, bu ...
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Phospholipid-derived fatty acids



Phospholipid-derived fatty acids (PLFA) are widely used in microbial ecology as chemotaxonomic markers of bacteria and other organisms. Phospholipids are the primary lipids composing cellular membranes. Phospholipids can be saponified, which releases the fatty acids contained in their diglyceride tail. Once the phospholipids of an unknown sample are saponified, the composition of the resulting PLFA can be compared to the PLFA of known organisms to determine the identity of the sample organism. PLFA analysis may be combined with other techniques, such as stable isotope probing to determine which microbes are metabolically active in a sample. PLFA analysis was pioneered by D.C. White, MD, PhD, at the University of Tennessee, in the early to mid 1980s.
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