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T -I O -D
T -I O -D

... clear view of naturally-occurring phenomena. Soil samples are collected from wetland habitats, amended with simple inorganic and organic materials, then exposed to light as an external energy source. The results are a multicolored column of soil and water, each color linked to a chemical or biologic ...
Host-Microbiota Interactions in the Gut
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Phospholipid-derived fatty acids



Phospholipid-derived fatty acids (PLFA) are widely used in microbial ecology as chemotaxonomic markers of bacteria and other organisms. Phospholipids are the primary lipids composing cellular membranes. Phospholipids can be saponified, which releases the fatty acids contained in their diglyceride tail. Once the phospholipids of an unknown sample are saponified, the composition of the resulting PLFA can be compared to the PLFA of known organisms to determine the identity of the sample organism. PLFA analysis may be combined with other techniques, such as stable isotope probing to determine which microbes are metabolically active in a sample. PLFA analysis was pioneered by D.C. White, MD, PhD, at the University of Tennessee, in the early to mid 1980s.
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