PARP-14 combines with tristetraprolin in the selective
... coactivator of signal transducer and activator of transcription6–mediated gene transcription in B cells.24-26 Although studies to date on PARP proteins have mainly focused on their nuclear activities, PARP-14 is also expressed, along with several other PARP proteins, in the cytoplasm and may have ro ...
... coactivator of signal transducer and activator of transcription6–mediated gene transcription in B cells.24-26 Although studies to date on PARP proteins have mainly focused on their nuclear activities, PARP-14 is also expressed, along with several other PARP proteins, in the cytoplasm and may have ro ...
The Virulence Function of Streptococcus pneumoniae
... It is currently accepted that three different pathways can lead to complement activation: the classical pathway, the mannose-binding lectin (MBL) pathway, and the alternative pathway (8). All three pathways converge upon activation of C3, which is cleaved into C3a and C3b. This process exposes a thi ...
... It is currently accepted that three different pathways can lead to complement activation: the classical pathway, the mannose-binding lectin (MBL) pathway, and the alternative pathway (8). All three pathways converge upon activation of C3, which is cleaved into C3a and C3b. This process exposes a thi ...
HLA typing in transfusion and transplantation
... Cross-Reactive Groups • Systems have been developed to match donor and recipient by assigning HLA-A and HLA-B antigens with shared public epitopes to clusters called cross-reactive groups (CREGs). • When one or two mismatches of HLA-A or HLAB antigens in CREGs is permitted, a pool of 1000– 3000 don ...
... Cross-Reactive Groups • Systems have been developed to match donor and recipient by assigning HLA-A and HLA-B antigens with shared public epitopes to clusters called cross-reactive groups (CREGs). • When one or two mismatches of HLA-A or HLAB antigens in CREGs is permitted, a pool of 1000– 3000 don ...
Skeleton-binding protein 1 functions at the
... Synchronized trophozoite cultures were saponin lysed, and the pellet was washed 3 times in PBS and taken up in SDS sample buffer (Invitrogen). Proteins were separated on 3% to 8% Tris-Acetate or 10% Bis-Tris gels (Invitrogen, Carlsbad, CA). Western blotting to nitrocellulose (0.45 m; Schleicher and ...
... Synchronized trophozoite cultures were saponin lysed, and the pellet was washed 3 times in PBS and taken up in SDS sample buffer (Invitrogen). Proteins were separated on 3% to 8% Tris-Acetate or 10% Bis-Tris gels (Invitrogen, Carlsbad, CA). Western blotting to nitrocellulose (0.45 m; Schleicher and ...
A three base pair gene variation within the distal 5`
... ABSTRACT. Interleukin-10 (IL-10) is an important multifunctional immunmodulator. There is evidence that IL-10 secretion is associated with certain genetic elements of the proximal IL-10 gene 5’-flanking region. The allelic and genotypic comparison of IL-10 expression by lipopolysaccharide (LPS)- sti ...
... ABSTRACT. Interleukin-10 (IL-10) is an important multifunctional immunmodulator. There is evidence that IL-10 secretion is associated with certain genetic elements of the proximal IL-10 gene 5’-flanking region. The allelic and genotypic comparison of IL-10 expression by lipopolysaccharide (LPS)- sti ...
Erythropoietin gene from a teleost fish, Fugu rubripes
... 2 of these genes, PERQ1 and RPP20, have been mapped to the human EPO locus (University of California, Santa Cruz [UCSC] Human Genome Gateway at http://genome.ucsc.edu) upstream of the EPO gene (Figure 1B). BLAST searching of the human genome sequence with the other Fugu genes from the Epo locus unco ...
... 2 of these genes, PERQ1 and RPP20, have been mapped to the human EPO locus (University of California, Santa Cruz [UCSC] Human Genome Gateway at http://genome.ucsc.edu) upstream of the EPO gene (Figure 1B). BLAST searching of the human genome sequence with the other Fugu genes from the Epo locus unco ...
Selection of Potentially Probiotic Lactobacillus Strains Towards their
... Materials and Methods Bacteria and growth conditions. Lactobacilli were isolated from faeces and intestines of healthy chickens. Samples were inoculated into Rogosa medium (Merck). The plates were incubated at 37°C for 48 h under anaerobic conditions generated by BBLTM GasPak system (Becton Dickinso ...
... Materials and Methods Bacteria and growth conditions. Lactobacilli were isolated from faeces and intestines of healthy chickens. Samples were inoculated into Rogosa medium (Merck). The plates were incubated at 37°C for 48 h under anaerobic conditions generated by BBLTM GasPak system (Becton Dickinso ...
EndoS and SpeB from Streptococcus pyogenes
... protein is the well-characterized streptococcal cysteine proteinase SpeB (17). Recently, the IgG-hydrolyzing activity of SpeB, which cleaves human IgG into Fc and monomeric Fab fragments, was described (6). In the same report, a novel extracellular enzyme from S. pyogenes, designated EndoS, was iden ...
... protein is the well-characterized streptococcal cysteine proteinase SpeB (17). Recently, the IgG-hydrolyzing activity of SpeB, which cleaves human IgG into Fc and monomeric Fab fragments, was described (6). In the same report, a novel extracellular enzyme from S. pyogenes, designated EndoS, was iden ...
Characterization of Haemolysin of Staphylococcus aureus Isolated
... with two genes in combination hla and hlb (18,18%). The size of amplicon for hla gene was approximately 534 bp and 833 bp for hlb gene (Figure 2 and 3). The S. aureus isolates with hla genes expressed alpha-, beta- and gamma-haemolysis on the sheep blood agar. The S. aureus with two genes in combina ...
... with two genes in combination hla and hlb (18,18%). The size of amplicon for hla gene was approximately 534 bp and 833 bp for hlb gene (Figure 2 and 3). The S. aureus isolates with hla genes expressed alpha-, beta- and gamma-haemolysis on the sheep blood agar. The S. aureus with two genes in combina ...
HEMOSTASIS
... The fibrinogen group includes fibrinogen (factor I) and factor V, VIII, and XIII. These have the highest molecular weights of all factors, are the most labile, are consumed in coagulation, and are the only group that act as substrates for the fibrinolytic enzyme plasmin. Only the factors found in th ...
... The fibrinogen group includes fibrinogen (factor I) and factor V, VIII, and XIII. These have the highest molecular weights of all factors, are the most labile, are consumed in coagulation, and are the only group that act as substrates for the fibrinolytic enzyme plasmin. Only the factors found in th ...
ACE and ARB FAQ - South London Cardiac and Stroke Network
... paramount importance in this patient group. However, proteinuria is itself a risk factor for CV events, and patients with diabetic kidney disease are at incrementally higher CV risk. Treatment strategies should therefore aim to protect against CV events and preserve renal function in this group of p ...
... paramount importance in this patient group. However, proteinuria is itself a risk factor for CV events, and patients with diabetic kidney disease are at incrementally higher CV risk. Treatment strategies should therefore aim to protect against CV events and preserve renal function in this group of p ...
Urinalysis Reagent Strips U031-101 (Urine)
... Ascorbic acid: This test involves decolorization of Tillmann’s reagent. The presence of ascorbic acid causes the color of the test field to change from blue-green to orange. Patients with adequate diet may excrete 2 - 10 mg/dL daily. After ingesting large amounts of ascorbic acid, levels can be arou ...
... Ascorbic acid: This test involves decolorization of Tillmann’s reagent. The presence of ascorbic acid causes the color of the test field to change from blue-green to orange. Patients with adequate diet may excrete 2 - 10 mg/dL daily. After ingesting large amounts of ascorbic acid, levels can be arou ...
Molecular Analysis of Polish Patients With Factor VI1
... pattern of the disorder implies that severely affected individuals can be homozygous or doubly heterozygous. The molecular basis of factor VI1 deficiency, in contrast to the relatively common sex-linked hemophilias, has only recently begun to be investigated, and most of the reported mutations have ...
... pattern of the disorder implies that severely affected individuals can be homozygous or doubly heterozygous. The molecular basis of factor VI1 deficiency, in contrast to the relatively common sex-linked hemophilias, has only recently begun to be investigated, and most of the reported mutations have ...
Molecular Analysis of Polish Patients With Factor VI1
... pattern of the disorder implies that severely affected individuals can be homozygous or doubly heterozygous. The molecular basis of factor VI1 deficiency, in contrast to the relatively common sex-linked hemophilias, has only recently begun to be investigated, and most of the reported mutations have ...
... pattern of the disorder implies that severely affected individuals can be homozygous or doubly heterozygous. The molecular basis of factor VI1 deficiency, in contrast to the relatively common sex-linked hemophilias, has only recently begun to be investigated, and most of the reported mutations have ...
Recessively inherited coagulation disorders
... Expression of a number of mutations in cultured mammalian cells and characterization of the trafficking and secretion of the corresponding recombinant proteins has helped to explain how they lead to factor deficiency. In some instances—as, for example, due to missense mutations in the genes of fibri ...
... Expression of a number of mutations in cultured mammalian cells and characterization of the trafficking and secretion of the corresponding recombinant proteins has helped to explain how they lead to factor deficiency. In some instances—as, for example, due to missense mutations in the genes of fibri ...
Get PDF - Wiley Online Library
... synthesis of new protein factors [17]. These mechanisms are likely to be shared by other cold-shockspeci¢c genes in the cspA family such as cspB, cspG and the newly characterized cspI [4], since they all have 5P-UTRs of comparable length. ...
... synthesis of new protein factors [17]. These mechanisms are likely to be shared by other cold-shockspeci¢c genes in the cspA family such as cspB, cspG and the newly characterized cspI [4], since they all have 5P-UTRs of comparable length. ...
High-level production of membrane proteins in E
... more intense than the ones of cells cultured in the presence of IPTG, which is in keeping with the whole cell fluorescence measurements. Next, we used an SDS-PAGE/immuno-blotting-based assay that can distinguish between membrane integrated and non-integrated material (see also Fig. 1) [23]. The ass ...
... more intense than the ones of cells cultured in the presence of IPTG, which is in keeping with the whole cell fluorescence measurements. Next, we used an SDS-PAGE/immuno-blotting-based assay that can distinguish between membrane integrated and non-integrated material (see also Fig. 1) [23]. The ass ...
View - OhioLINK Electronic Theses and Dissertations Center
... complement-mediated lysis. LVSG and LVSR are variant strains derived from LVS that have altered surface carbohydrate structures and are susceptible to complement-mediated lysis in serum. C3b deposition, however, occurs on each strain tested, indicating that complement is not solely activated by var ...
... complement-mediated lysis. LVSG and LVSR are variant strains derived from LVS that have altered surface carbohydrate structures and are susceptible to complement-mediated lysis in serum. C3b deposition, however, occurs on each strain tested, indicating that complement is not solely activated by var ...
Haematological Indices and Blood Urea Nitrogen of Yankasa Ram
... Ether extract contents obtained from feeds in the present study were higher than 4.5 - 3.40% used by Maigandi and Tukur (2002) in the diet of growing lambs. It is expected that growth performance of the ram lambs will be supported by presence of higher values of crude protein or ether extract in tre ...
... Ether extract contents obtained from feeds in the present study were higher than 4.5 - 3.40% used by Maigandi and Tukur (2002) in the diet of growing lambs. It is expected that growth performance of the ram lambs will be supported by presence of higher values of crude protein or ether extract in tre ...
Antimicrobials 101
... CAP Major problems: GI issues, and shown to have increased mortality in serious infections – monotherapy only as a last resort. ...
... CAP Major problems: GI issues, and shown to have increased mortality in serious infections – monotherapy only as a last resort. ...
specificity of brucella protein antigens and role of - HAL
... Studies in sensitized guinea pigs (table i) showed that protein antigens prepared from B. canis, B. ovis and B. melitensis B 115 had approximately the same reactivity. Intradermal doses of i (Jog of each of the antigens produced erythematous reactions of about 15 mm in diameter in guinea pigs skin t ...
... Studies in sensitized guinea pigs (table i) showed that protein antigens prepared from B. canis, B. ovis and B. melitensis B 115 had approximately the same reactivity. Intradermal doses of i (Jog of each of the antigens produced erythematous reactions of about 15 mm in diameter in guinea pigs skin t ...
Recombinant Sickle Hemoglobin Containing a Lysine
... Most of the earlier work with the recombinant Hb system focused on the sites at or near the hydrophobic pocket of the beta chain, which is the acceptor for the mutated Val residue.18-20 Recently, we showed that a residue located at the exterior of the Hb tetramer, (Lys-95[b]),21 reduces the gelation ...
... Most of the earlier work with the recombinant Hb system focused on the sites at or near the hydrophobic pocket of the beta chain, which is the acceptor for the mutated Val residue.18-20 Recently, we showed that a residue located at the exterior of the Hb tetramer, (Lys-95[b]),21 reduces the gelation ...
Biosketch
... cloning of tissue factor in 1987.1 Currently, my lab focuses on two major areas of investigation: (1) Proteinmembrane interactions in clotting. Recent advances now enable the study of protein-membrane interactions with unprecedented resolution, including nanoscale phospholipid bilayers (collaboratio ...
... cloning of tissue factor in 1987.1 Currently, my lab focuses on two major areas of investigation: (1) Proteinmembrane interactions in clotting. Recent advances now enable the study of protein-membrane interactions with unprecedented resolution, including nanoscale phospholipid bilayers (collaboratio ...
Characterization of Antimicrobial Substance Produced by
... The KNUC25 strain isolated from over-fermented whole Chinese cabbage kimchi was examined for its physiological characteristics using API 50 CHL system assay and identified as Lactobacillus paraplantarum by analysis of whole-cell protein SDS-PAGE pattern assay and similarity of 16S rDNA sequence. L. ...
... The KNUC25 strain isolated from over-fermented whole Chinese cabbage kimchi was examined for its physiological characteristics using API 50 CHL system assay and identified as Lactobacillus paraplantarum by analysis of whole-cell protein SDS-PAGE pattern assay and similarity of 16S rDNA sequence. L. ...
Calcitonin (F-13): sc-9174
... Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid ...
... Calcitonin is a 32 amino acid polypeptide hormone that preserves skeletal integrity and reduces blood calcium levels by decreasing osteoclast activity in bones, calcium and phosphate reabsorption by kidney tubules and calcium absorption by the intestines. The secretion of Calcitonin from the thyroid ...
Complement component 4
Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a: C4b can bind CR1.