6. 7. 8. Brief Resume of the Intended Work
... With the trend towards the generation and production of increasing number of complex biopharmaceutical (protein based) products, there is an increased need and requirement to characterize both the product and production process in terms of robustness and reproducibility. Characterization of the Drug ...
... With the trend towards the generation and production of increasing number of complex biopharmaceutical (protein based) products, there is an increased need and requirement to characterize both the product and production process in terms of robustness and reproducibility. Characterization of the Drug ...
Purification of a Human Red Blood Cell Protein
... adjusted to pH 7.2 and then submitted to a second DEAE column. Each fraction was assayed for protein and neuronotrophic activity; fraction sizes were 16.5 ml for eluate, 10 ml for gradient, and 1 M NaCI. A substantial portion of the recoverable trophic units (histogram) was eluted in two fractions w ...
... adjusted to pH 7.2 and then submitted to a second DEAE column. Each fraction was assayed for protein and neuronotrophic activity; fraction sizes were 16.5 ml for eluate, 10 ml for gradient, and 1 M NaCI. A substantial portion of the recoverable trophic units (histogram) was eluted in two fractions w ...
Acute phase response in Chinese soft
... protein of 127 aa. Similar to other known SAA genes, the turtle SAA gene contained three exons and two introns. The promoter region of turtle SAA gene contained the consensus binding sites for nuclear factor (NF)-B and c-Rel. The turtle SAA amino acid sequence shared the highest identity to avian S ...
... protein of 127 aa. Similar to other known SAA genes, the turtle SAA gene contained three exons and two introns. The promoter region of turtle SAA gene contained the consensus binding sites for nuclear factor (NF)-B and c-Rel. The turtle SAA amino acid sequence shared the highest identity to avian S ...
TC16100 - Technozym® Protein C INH Actibind®
... PCI inhibits its target proteases by forming SDS stable 1:1 complexes. Upon complex formation the reactive site peptide bond of the inhibitor is cleaved by the protease and the carboxy-terminal peptide is released from the inhibitor. Depending on the target protease, complexes dissociate more or les ...
... PCI inhibits its target proteases by forming SDS stable 1:1 complexes. Upon complex formation the reactive site peptide bond of the inhibitor is cleaved by the protease and the carboxy-terminal peptide is released from the inhibitor. Depending on the target protease, complexes dissociate more or les ...
Genetics of Essential Hypertension: From Families to Genes
... accounts for 10% of the total genetic variation; our apparent negligible value of 4% becomes a more relevant 10% of the total genetic variation. However, we must be aware of the fact that the exact relationship between the h2 value and the genetic-molecular mechanism at work in that population can b ...
... accounts for 10% of the total genetic variation; our apparent negligible value of 4% becomes a more relevant 10% of the total genetic variation. However, we must be aware of the fact that the exact relationship between the h2 value and the genetic-molecular mechanism at work in that population can b ...
Pocket card - Roche Diagnostics (Schweiz)
... Binds factor VIIa which initiates the tissue factor/extrinsic pathway Activated to factor Va which acts as a cofactor for the enzyme factor Xa that cleaves prothrombin to thrombin Present in granules in platelets Binds to tissue factor to initiate the tissue factor/extrinsic pathway ...
... Binds factor VIIa which initiates the tissue factor/extrinsic pathway Activated to factor Va which acts as a cofactor for the enzyme factor Xa that cleaves prothrombin to thrombin Present in granules in platelets Binds to tissue factor to initiate the tissue factor/extrinsic pathway ...
125
... Antibodies are protein molecules formed by the B lymphocytes of the vertebrate immune system that recognize and bind to specific molecular epitopes (termed antigens). A wide variety of substances can serve as antigens including proteins, carbohydrates, simple organic molecules, or even metal ions. Th ...
... Antibodies are protein molecules formed by the B lymphocytes of the vertebrate immune system that recognize and bind to specific molecular epitopes (termed antigens). A wide variety of substances can serve as antigens including proteins, carbohydrates, simple organic molecules, or even metal ions. Th ...
Red blood cell destruction in autoimmune hemolytic anemia: Role of
... hypothyroidism, inflammatory bowel disease, immune thrombocytopenia and primary hypogammaglobulinemia [1,2,15,17]. Some patients have several associated diseases at the same time. Autoantibody or complement fragment deposition on the RBC can be detected using polyspecific and monospecific direct ant ...
... hypothyroidism, inflammatory bowel disease, immune thrombocytopenia and primary hypogammaglobulinemia [1,2,15,17]. Some patients have several associated diseases at the same time. Autoantibody or complement fragment deposition on the RBC can be detected using polyspecific and monospecific direct ant ...
organic chemistry
... www.smarteduhub.com (c) The alkane with the molecular formula C5H12 can exist as a number of structural isomers. Draw the structures of two isomers with the formula C5H12. ...
... www.smarteduhub.com (c) The alkane with the molecular formula C5H12 can exist as a number of structural isomers. Draw the structures of two isomers with the formula C5H12. ...
Importance of individual activated protein C cleavage site regions in
... APC resistance and increased thrombotic risk are due to the mutation R506Q in Factor V (FV). To study the effects of individual cleavages in FVa by APC and the importance of regions near the cleavage sites, the following recombinant (r) human FVs were prepared and purified: wild-type, Q306-rFV, Q506 ...
... APC resistance and increased thrombotic risk are due to the mutation R506Q in Factor V (FV). To study the effects of individual cleavages in FVa by APC and the importance of regions near the cleavage sites, the following recombinant (r) human FVs were prepared and purified: wild-type, Q306-rFV, Q506 ...
Characterization of β-globin haplotypes using blood spots from a
... areas of the world, make the -globin gene cluster particularly useful for studies of human evolution.13–18 In addition to its contribution to population genetics, the study of the -globin gene cluster has also proven valuable in the study of disorders that are modified by other genes. Although sic ...
... areas of the world, make the -globin gene cluster particularly useful for studies of human evolution.13–18 In addition to its contribution to population genetics, the study of the -globin gene cluster has also proven valuable in the study of disorders that are modified by other genes. Although sic ...
الشريحة 1
... After ABO, the Rh system is the second most important system. This is because: The D antigen is extremely immunogenic. It causes the production of anti-D in 50 - 70% of Rh(D) negative people who are exposed to the D antigen. Moreover, anti-D is the most common cause of severe HDN and can cause in Ut ...
... After ABO, the Rh system is the second most important system. This is because: The D antigen is extremely immunogenic. It causes the production of anti-D in 50 - 70% of Rh(D) negative people who are exposed to the D antigen. Moreover, anti-D is the most common cause of severe HDN and can cause in Ut ...
A family of textilinin genes, two of which encode proteins with
... venom of Pseudonaja textilis textilis that inhibit plasmin activity and reduce blood loss in an experimental animal model (Masci et al, 2000). Based on the protein sequences for Txln 1 and 2, we designed degenerate primers to amplify a fragment of approximately 180 bp using RT-PCR with total RNA iso ...
... venom of Pseudonaja textilis textilis that inhibit plasmin activity and reduce blood loss in an experimental animal model (Masci et al, 2000). Based on the protein sequences for Txln 1 and 2, we designed degenerate primers to amplify a fragment of approximately 180 bp using RT-PCR with total RNA iso ...
A Novel 0-Globin Mutation, @Durham-NC [@l 14
... cells, basophilic stippling, and occasional target cells (Fig 2). No inclusion bodies were noted. Further workup included a cloned to confirm that any sequence difference was not due to Taq misincorporation. Cloned exon 3 fragments were reticulocyte count of 94 X 106/gL, a total bilirubin level of t ...
... cells, basophilic stippling, and occasional target cells (Fig 2). No inclusion bodies were noted. Further workup included a cloned to confirm that any sequence difference was not due to Taq misincorporation. Cloned exon 3 fragments were reticulocyte count of 94 X 106/gL, a total bilirubin level of t ...
Light-sensitive `Jaws` protein could lead to more sensitive optogenetics
... Aiming for more sensitive optogenetics, Massachusetts Institute of Technology (MIT; Cambridge, MA) engineers have developed the first light-sensitive molecule that enables neurons to be silenced noninvasively. By using a light source outside the skull, the work makes it possible to do long-term stud ...
... Aiming for more sensitive optogenetics, Massachusetts Institute of Technology (MIT; Cambridge, MA) engineers have developed the first light-sensitive molecule that enables neurons to be silenced noninvasively. By using a light source outside the skull, the work makes it possible to do long-term stud ...
Influence of Atomic and Singlet Molecular Oxygen Generated by RF
... properties of permeability to sterilized objects. The reaction between oxygen plasma and the sample was not selective because the reduction rate indicated almost same tendency between typical peaks (1650cm-1, 1545cm-1 and 3300 cm-1) of protein (Fig. 5). Oxygen sterilization possibly has difficulties ...
... properties of permeability to sterilized objects. The reaction between oxygen plasma and the sample was not selective because the reduction rate indicated almost same tendency between typical peaks (1650cm-1, 1545cm-1 and 3300 cm-1) of protein (Fig. 5). Oxygen sterilization possibly has difficulties ...
Site-specific gene correction of a point mutation in human iPS cells
... 116) of the HBB gene containing the putative ZFN recognition sites into the middle of the EGIP* vector following a previously published strategy.3 The insertion, together with a stop codon (taa) upstream and a HindIII site downstream aagctt), disrupts the enhanced green fluorescent protein (EGFP) re ...
... 116) of the HBB gene containing the putative ZFN recognition sites into the middle of the EGIP* vector following a previously published strategy.3 The insertion, together with a stop codon (taa) upstream and a HindIII site downstream aagctt), disrupts the enhanced green fluorescent protein (EGFP) re ...
FEMS Microbiology Letters
... In this work, we report the determination of AAT activity and detection after non-denaturing and denaturing PAGE from crude cell-free extracts of nine N2 fixing bacterial strains (Table 1). Enzymatic activity, specific activity, and number of isoforms of the nine crude cell-free extracts used in this ...
... In this work, we report the determination of AAT activity and detection after non-denaturing and denaturing PAGE from crude cell-free extracts of nine N2 fixing bacterial strains (Table 1). Enzymatic activity, specific activity, and number of isoforms of the nine crude cell-free extracts used in this ...
by-products used for feeding dairy cattle
... CORN GLUTEN FEED consists of corn gluten and corn bran, with or without corn solubles. It is a good source of protein (approximately 25 percent). When and where cost is not prohibitive, corn gluten feed may be used in the grain mix without restriction. CORN GLUTEN MEAL consists mainly of the corn gl ...
... CORN GLUTEN FEED consists of corn gluten and corn bran, with or without corn solubles. It is a good source of protein (approximately 25 percent). When and where cost is not prohibitive, corn gluten feed may be used in the grain mix without restriction. CORN GLUTEN MEAL consists mainly of the corn gl ...
Hemostasis
... MOST COMMON congenital bleeding disorder Low levels of vWFvariable decrease in Factor VIII due to loss of the carrier protein vWF is necessary for normal platelet aggregation; therefore deficiency presents in a similar fashion to platelet disorders Prolonged bleeding time, possible abnormal ...
... MOST COMMON congenital bleeding disorder Low levels of vWFvariable decrease in Factor VIII due to loss of the carrier protein vWF is necessary for normal platelet aggregation; therefore deficiency presents in a similar fashion to platelet disorders Prolonged bleeding time, possible abnormal ...
Wiskott-Aldrich Syndrome/X-Linked
... (XLT).7-11 In patients with XLT, eczema is mild, if present, and immune functions may be normal. The genes for both WAS and XLT have been mapped to the pericentromeric short arm of the X chromosome at Xp 11.22,12,13 and sequence analysis of the WASP gene has identified mutations of the same gene in ...
... (XLT).7-11 In patients with XLT, eczema is mild, if present, and immune functions may be normal. The genes for both WAS and XLT have been mapped to the pericentromeric short arm of the X chromosome at Xp 11.22,12,13 and sequence analysis of the WASP gene has identified mutations of the same gene in ...
Production of empty Turnip yellow mosaic virus capsids from
... the ori for both E. coli and Lactococcus. • The sequence will then be verified by restriction digest and sequencing the gene of interest. • The Lactococcus will then be induced to produce the empty TYMV capsid. ...
... the ori for both E. coli and Lactococcus. • The sequence will then be verified by restriction digest and sequencing the gene of interest. • The Lactococcus will then be induced to produce the empty TYMV capsid. ...
Blood Group Terminology 1990 - International Society of Blood
... alleles of a single gene. If all resources are used, exclusion from the established systems of an antigen that is controlled by a ‘polymorphic’ gene (at least one allele having an incidence of >1%~ 9 9 % is) feasible unless the locus is contiguous, or very closely linked, to one of those loci involv ...
... alleles of a single gene. If all resources are used, exclusion from the established systems of an antigen that is controlled by a ‘polymorphic’ gene (at least one allele having an incidence of >1%~ 9 9 % is) feasible unless the locus is contiguous, or very closely linked, to one of those loci involv ...
New Insights on hereditary erythrocyte membrane
... is certainly the membrane, that ensures both mechanical stability and deformability. After the first proposed model of the RBC membrane skeleton 36 years ago,1 containing the core elements of the modern model, many additional proteins have been discovered during the intervening decades, and their st ...
... is certainly the membrane, that ensures both mechanical stability and deformability. After the first proposed model of the RBC membrane skeleton 36 years ago,1 containing the core elements of the modern model, many additional proteins have been discovered during the intervening decades, and their st ...
Complement component 4
Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a: C4b can bind CR1.