6.lect PKU NEW
... • In cases of PKU, the enzyme that breaks down phenylalanine, phenylalanine hydroxylase, or of its tetrahydrobiopterin cofactor which may be completely or nearly completely deficient. ...
... • In cases of PKU, the enzyme that breaks down phenylalanine, phenylalanine hydroxylase, or of its tetrahydrobiopterin cofactor which may be completely or nearly completely deficient. ...
Glycomacropeptide for nutritional management of phenylketonuria
... their low-Phe diet with emphasis on avoiding high-Phe foods within their daily Phe allowance and consuming sufficient amounts of AA-MFs in $3 servings/d. Subjects consumed their preferred AA-MFs resulting in the use of 15 different types of Phe-free AA-MFs as shown in Supplemental Table 1. ...
... their low-Phe diet with emphasis on avoiding high-Phe foods within their daily Phe allowance and consuming sufficient amounts of AA-MFs in $3 servings/d. Subjects consumed their preferred AA-MFs resulting in the use of 15 different types of Phe-free AA-MFs as shown in Supplemental Table 1. ...
Glycomacropeptide for nutritional management of phenylketonuria
... concentration of Phe in subjects ingesting GMP-MFs compared with the change when ingesting AA-MFs, calculated as the difference between the final Phe concentration at day 22 and the baseline Phe concentration at day 1. The trial was powered at 80% (b = 0.20) based on a previously reported SD of 150 ...
... concentration of Phe in subjects ingesting GMP-MFs compared with the change when ingesting AA-MFs, calculated as the difference between the final Phe concentration at day 22 and the baseline Phe concentration at day 1. The trial was powered at 80% (b = 0.20) based on a previously reported SD of 150 ...
- Wiley Online Library
... [w/v]) of Amberlite XAD-16 (Sigma) was added to the supernatant, and the sample kept at 4 °C with stirring for 2 h. The sample was loaded into an econo-column chromatography support (Bio-Rad) and, when the supernatant drained off, the matrix was washed with 100 ml distilled water in the first step and ...
... [w/v]) of Amberlite XAD-16 (Sigma) was added to the supernatant, and the sample kept at 4 °C with stirring for 2 h. The sample was loaded into an econo-column chromatography support (Bio-Rad) and, when the supernatant drained off, the matrix was washed with 100 ml distilled water in the first step and ...
An association of candidate gene haplotypes and
... between collagen and the glycoprotein Ib (GPIb) complex.1 Next, the direct binding of platelet ␣21 and platelet GPVI to collagen anchors the platelet more firmly to the matrix and facilitates signal transduction that leads to an activated, procoagulant platelet monolayer.2 Genetic variation in any ...
... between collagen and the glycoprotein Ib (GPIb) complex.1 Next, the direct binding of platelet ␣21 and platelet GPVI to collagen anchors the platelet more firmly to the matrix and facilitates signal transduction that leads to an activated, procoagulant platelet monolayer.2 Genetic variation in any ...
Document
... Question 3: Given the following DNA binding motif: Helix2-turn-Helix3 for a homeodomain protein. Trp48, Phe49, Asn 51 and Arg53 are invariant amongst almost all homeodomains in order to retain DNA binding. However, you want to test this idea with single amino acid substitutions. (2pts for 3 correct) ...
... Question 3: Given the following DNA binding motif: Helix2-turn-Helix3 for a homeodomain protein. Trp48, Phe49, Asn 51 and Arg53 are invariant amongst almost all homeodomains in order to retain DNA binding. However, you want to test this idea with single amino acid substitutions. (2pts for 3 correct) ...
Vascular Injury: Overview Hemostasis Hemostasis Blood vessels
... Von Willebrand Factor • A large, multimeric plasma glycoprotein – Produced mainly by endothelial cells • Secreted constituitively or stored and released upon endothelial d th li l activation ti ti • Also produced by megakaryocytes and is present in platelets ...
... Von Willebrand Factor • A large, multimeric plasma glycoprotein – Produced mainly by endothelial cells • Secreted constituitively or stored and released upon endothelial d th li l activation ti ti • Also produced by megakaryocytes and is present in platelets ...
Specific IgE to colophony? - Oasys and Occupational Asthma
... able to detect increased binding of specific IgE antibody in serum from individuals who were exposed, symptomatic and have a high likelihood of occupational asthma (supported by clinical evidence). Although the number of serum samples was small, conjugates produced from a range of colophony sources ...
... able to detect increased binding of specific IgE antibody in serum from individuals who were exposed, symptomatic and have a high likelihood of occupational asthma (supported by clinical evidence). Although the number of serum samples was small, conjugates produced from a range of colophony sources ...
Progress in understanding the assembly process of bacterial O
... an ABC transporter-dependent mechanism (Valvano, 2003) (Fig. 1). The third pathway for O-antigen biosynthesis is known as the synthase-dependent mechanism and so far is known to be present only in one Gram-negative species (S. enterica serovar Borreze O:54) and little is presently know about its det ...
... an ABC transporter-dependent mechanism (Valvano, 2003) (Fig. 1). The third pathway for O-antigen biosynthesis is known as the synthase-dependent mechanism and so far is known to be present only in one Gram-negative species (S. enterica serovar Borreze O:54) and little is presently know about its det ...
Increased Erythropoiesis in Mice Injected With Submicrogram
... anti-EPO neutralizing antibodies and transfusion-dependent anemia.20,26 Thus, avoiding an immune reaction is essential for effective protein replacement, regardless if it is performed by directly supplying the protein or via an encoding vector. Using nucleosidemodified mRNA coding for the therapeuti ...
... anti-EPO neutralizing antibodies and transfusion-dependent anemia.20,26 Thus, avoiding an immune reaction is essential for effective protein replacement, regardless if it is performed by directly supplying the protein or via an encoding vector. Using nucleosidemodified mRNA coding for the therapeuti ...
UniProtKB/Swiss-Prot and ExPASy: Protein
... all scientific research, DNA and protein annotation is a continual process of learning, revision and corrections.” “Sequencing error rates: ~1 base in 10’000” “Making people aware of errors is good and great; making people aware that they’re responsible also for correcting errors is even greater” ...
... all scientific research, DNA and protein annotation is a continual process of learning, revision and corrections.” “Sequencing error rates: ~1 base in 10’000” “Making people aware of errors is good and great; making people aware that they’re responsible also for correcting errors is even greater” ...
factor xiii: a coagulation factor with multiple
... 2011; doi:10.1152/physrev.00016.2010.—Factor XIII (FXIII) is unique among clotting factors for a number of reasons: 1) it is a protransglutaminase, which becomes activated in the last stage of coagulation; 2) it works on an insoluble substrate; 3) its potentially active subunit is also present in th ...
... 2011; doi:10.1152/physrev.00016.2010.—Factor XIII (FXIII) is unique among clotting factors for a number of reasons: 1) it is a protransglutaminase, which becomes activated in the last stage of coagulation; 2) it works on an insoluble substrate; 3) its potentially active subunit is also present in th ...
Enhanced circulating half-life and hematopoietic properties of a
... used to create a number of long-acting fusion proteins [11]. Therapeutic proteins typically have been joined via their carboxy-termini to the amino-termini of Fc (Hinge-CH2CH3) and CH (CH1-Hinge-CH2-CH3) domains of human IgGs. When expressed in mammalian cells IgG fusion proteins often are secreted ...
... used to create a number of long-acting fusion proteins [11]. Therapeutic proteins typically have been joined via their carboxy-termini to the amino-termini of Fc (Hinge-CH2CH3) and CH (CH1-Hinge-CH2-CH3) domains of human IgGs. When expressed in mammalian cells IgG fusion proteins often are secreted ...
Copra Meal Hydrolysis: A Review
... Copra meal can be hydrolyzed not only with acid but also with enzymes. Mannanases are enzymes that can hydrolyze mannan, galactomannans, or even glucomannans into their component oligosaccharides and monosaccharides. They have been isolated from animals, plants, and microorganisms (reviewed by Dekke ...
... Copra meal can be hydrolyzed not only with acid but also with enzymes. Mannanases are enzymes that can hydrolyze mannan, galactomannans, or even glucomannans into their component oligosaccharides and monosaccharides. They have been isolated from animals, plants, and microorganisms (reviewed by Dekke ...
Bacteriocins from plant pathogenic bacteria
... As judged by genome sequences, many plant pathogenic bacteria appear to contain genes encoding so-called RTXtoxins, in particular haemolysins, which are usually considered virulence factors of Gram-negative bacteria (Lally et al., 1999; van Sluys et al., 2002). Interestingly, Oresnik et al. (1999) s ...
... As judged by genome sequences, many plant pathogenic bacteria appear to contain genes encoding so-called RTXtoxins, in particular haemolysins, which are usually considered virulence factors of Gram-negative bacteria (Lally et al., 1999; van Sluys et al., 2002). Interestingly, Oresnik et al. (1999) s ...
Characterisation and co-expression of the two outer serotype 3
... proteins; VP1, VP4 and VP6 surrounded by two major core proteins VP3 and VP7. The outer capsid consists of two major outer capsid proteins, VP2 and VP5. VP2 has been shown to carry neutralising serotype specific epitopes. The VP2 protein is therefore a candidate for the development of subunit vaccin ...
... proteins; VP1, VP4 and VP6 surrounded by two major core proteins VP3 and VP7. The outer capsid consists of two major outer capsid proteins, VP2 and VP5. VP2 has been shown to carry neutralising serotype specific epitopes. The VP2 protein is therefore a candidate for the development of subunit vaccin ...
Binding of Soluble Type I Collagen to Fibroblasts
... relative to the type I LRSC standard. The values given below the lines are the ratio of LRSC (#g)/peptide ~g) which produced 30% inhibition of t25I-LRSC binding in the same experiment. For all of the experiments, 1-2/~g of LRSC regularly produced 30% inhibition of binding. Inhibition ratios <0.1 are ...
... relative to the type I LRSC standard. The values given below the lines are the ratio of LRSC (#g)/peptide ~g) which produced 30% inhibition of t25I-LRSC binding in the same experiment. For all of the experiments, 1-2/~g of LRSC regularly produced 30% inhibition of binding. Inhibition ratios <0.1 are ...
Chapter 7 Hemoglobin: Portrait of a Protein in Action
... Changes in heme electronic structure upon oxygen binding are the basis for functional imaging studies The change in electronic structure that occurs when the iron ion moves into the plane of the porphyrin is paralleled by alterations in the magnetic properties of hemoglobin; these changes are the b ...
... Changes in heme electronic structure upon oxygen binding are the basis for functional imaging studies The change in electronic structure that occurs when the iron ion moves into the plane of the porphyrin is paralleled by alterations in the magnetic properties of hemoglobin; these changes are the b ...
Mouse Models in Coagulation
... that encode the proteins that generate and regulate coagulation. Mice with altered factors or altered expression of normal factors have also been produced by transgenic techniques. In this review, we summarize the analyses of these mouse models and compare these results to those obtained from people ...
... that encode the proteins that generate and regulate coagulation. Mice with altered factors or altered expression of normal factors have also been produced by transgenic techniques. In this review, we summarize the analyses of these mouse models and compare these results to those obtained from people ...
An Introduction to Haemophilia and related bleeding disorders
... 1 in 10,000 of the population has the condition called haemophilia A. Clotting factor VIII lacks activity. Another of the clotting ingredients is called factor IX. The activity of this factor is deficient in haemophilia B, also known as Christmas disease. Haemophilia A is approximately five times mo ...
... 1 in 10,000 of the population has the condition called haemophilia A. Clotting factor VIII lacks activity. Another of the clotting ingredients is called factor IX. The activity of this factor is deficient in haemophilia B, also known as Christmas disease. Haemophilia A is approximately five times mo ...
Positive and Negative Cis-Acting Elements Are
... In XeX-GM2, the GM2 gene was driven by a 450-bp Xenopus elongation factor (EF) 1a enhancer/promoter sequence.17 The 150-bp enhancer was removed by XhoI/SphI digestion to generate Xs-GM2. To generate construct G1DE-Xs-GM2, a 2,589-bp XhoI/ClaI (blunt-ended) fragment (from -2968 to -5552) containing t ...
... In XeX-GM2, the GM2 gene was driven by a 450-bp Xenopus elongation factor (EF) 1a enhancer/promoter sequence.17 The 150-bp enhancer was removed by XhoI/SphI digestion to generate Xs-GM2. To generate construct G1DE-Xs-GM2, a 2,589-bp XhoI/ClaI (blunt-ended) fragment (from -2968 to -5552) containing t ...
Factor XI deficiency in French Basques is caused
... chain is composed of 4 “apple domains,” each composed of 90 to 91 amino acids and stabilized by 3 disulfide bridges.6,7 Apple domain 1 contains the binding site for high–molecular weight kininogen, thrombin, and prothrombin8-10; apple domain 3 contains the binding site for factor IX and platelets11, ...
... chain is composed of 4 “apple domains,” each composed of 90 to 91 amino acids and stabilized by 3 disulfide bridges.6,7 Apple domain 1 contains the binding site for high–molecular weight kininogen, thrombin, and prothrombin8-10; apple domain 3 contains the binding site for factor IX and platelets11, ...
B. cereus
... group is weakly clonal. In particular, all five B. anthracis isolates analyzed had the same ST. The MLST scheme has a high level of resolution and should be an excellent tool for studying the population structure and epidemiology of the B. cereus group ...
... group is weakly clonal. In particular, all five B. anthracis isolates analyzed had the same ST. The MLST scheme has a high level of resolution and should be an excellent tool for studying the population structure and epidemiology of the B. cereus group ...
Total RNA Sequencing reveals microbial communities in
... unmapped sequence reads for microbial taxonomic classification. Using this pipeline, we are able to detect bacterial and archaeal phyla in blood using RNA sequencing (RNA-Seq) data. Careful analyses, including the use of positive and negative control datasets, suggest that ...
... unmapped sequence reads for microbial taxonomic classification. Using this pipeline, we are able to detect bacterial and archaeal phyla in blood using RNA sequencing (RNA-Seq) data. Careful analyses, including the use of positive and negative control datasets, suggest that ...
Complement component 4
Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a: C4b can bind CR1.