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20pts total
Quiz 3: Biol 302 Spring2011
Name:_______________
Question 1:
Draw and Label a Two Exon Complete Gene. 10pts-labeling 14 structures correctly,
8pts-labeling 10-13 structures correctly, 5pts-labeling 6-9 structures correctly. 0pts for less than
6 structures labeled correctly or for any inaccuracies.
5’
3’
Question 2:
The two differences between splicing of a transcript and gene recombination are:
1pt_______________________________
1pt_______________________________
Question 3:
Given the following DNA binding motif: Helix2-turn-Helix3 for a homeodomain protein.
Trp48, Phe49, Asn 51 and Arg53 are
invariant amongst almost all homeodomains
in order to retain DNA binding.
However, you want to test this idea with single
amino acid substitutions. (2pts for 3 correct)
What will you change Trp48 to: _____
What will you change Phe49 to: _____
What will you change Asn51 to: _____
What will you change Arg53 to: _____
Question 4: :Given the following patterns produced in Drosophila segments starting from #1(WT)
Fill in appropriate segment identity in boxes below.
WT
#1
Minus Antp
#1
#1
minusUbx
T2
Antp
GS
-
T2
Antp
A1
Ubx
A1
Ubx
T2
Antp
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
A2
AbdA
#1 plus overexpression of
Antp in all segments, 2pts
#1 plus overexpression of
Ubx in all segments, 2pts
#1 plus overexpression of
AbdA in all segments, 2pts
Extra Credit 2pts: The above results of segment identity can be explained in two ways
Either the _____________________________________________________ or
________________________________________________________________________