News in DNA/RNA electrophoresis: Midori
... A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA marker (MWD1) includes fragments ranging from 250-10,000 base pairs. ...
... A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA marker (MWD1) includes fragments ranging from 250-10,000 base pairs. ...
LNUC IV.A - UTK-EECS
... This allows the polymerase to extend the primer on each single strand so that it becomes a double strand. Pairs of long strands can rehybridize, but the lighter oligonucleotides tend to get there faster, so instead we get two double strands. As a result, the concentration of desired DNA has doubled. ...
... This allows the polymerase to extend the primer on each single strand so that it becomes a double strand. Pairs of long strands can rehybridize, but the lighter oligonucleotides tend to get there faster, so instead we get two double strands. As a result, the concentration of desired DNA has doubled. ...
Poster
... A SMART Team project supported by the National Institutes of Health (NIH) – National Center for Research Resources Science Education Partnership Award (NCRR-SEPA) ...
... A SMART Team project supported by the National Institutes of Health (NIH) – National Center for Research Resources Science Education Partnership Award (NCRR-SEPA) ...
Ankita Naik 6 Harvey Road, Hounslow TW4 5LU Email
... Isolating DNA and RNA from various samples, designing the primers, setting up gel electrophoresis, qualitative and quantitative analysis of DNA and RNA looking for life cycle of Daphnia, feeding them, isolate DNA from the Daphnia Molecular biology techniques including PCR, RT-PCR, cell culture, ...
... Isolating DNA and RNA from various samples, designing the primers, setting up gel electrophoresis, qualitative and quantitative analysis of DNA and RNA looking for life cycle of Daphnia, feeding them, isolate DNA from the Daphnia Molecular biology techniques including PCR, RT-PCR, cell culture, ...
Translation webquest
... The primary function of DNA is to provide a blueprint for protein construction in our bodies. Yet, the process of protein construction is a little more complex, because DNA needs to be made into RNA in order for the proteins to be assembled properly. The purpose of this worksheet is to provide you w ...
... The primary function of DNA is to provide a blueprint for protein construction in our bodies. Yet, the process of protein construction is a little more complex, because DNA needs to be made into RNA in order for the proteins to be assembled properly. The purpose of this worksheet is to provide you w ...
DNA etcTest Rev 07
... 16. DNA is a double stranded molecule. 17. The shape of DNA is a double helix. 18. If one side of a DNA molecule is CTAATGGT, the other side is GATTACCA. 19. In DNA replication, one side of the molecule serves as a template for making the other side. 20. If a mistake happens during DNA replication, ...
... 16. DNA is a double stranded molecule. 17. The shape of DNA is a double helix. 18. If one side of a DNA molecule is CTAATGGT, the other side is GATTACCA. 19. In DNA replication, one side of the molecule serves as a template for making the other side. 20. If a mistake happens during DNA replication, ...
One Gene-one polypeptide:
... Similarities between DNA replication and RNA transcription 1. Only one of the 2 DNA strands acts as a template to make a complementary RNA copy, instead of both as in replication 2. Only a small part of a DNA molecule—the sequence encoding a single gene—serves as a template, rather than all of both ...
... Similarities between DNA replication and RNA transcription 1. Only one of the 2 DNA strands acts as a template to make a complementary RNA copy, instead of both as in replication 2. Only a small part of a DNA molecule—the sequence encoding a single gene—serves as a template, rather than all of both ...
Nucleotides
... form the “backbone” of RNA and DNA • RNAs are far less stable than DNA • Polynucleotides Are Directional Macromolecule – “5′- end” or the “3′- end” – the 5′- end is at the left ...
... form the “backbone” of RNA and DNA • RNAs are far less stable than DNA • Polynucleotides Are Directional Macromolecule – “5′- end” or the “3′- end” – the 5′- end is at the left ...
2. You perform a Southern blot in which your probe should hybridize
... hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available for hybridization as it is inside cells, and not denatured.} 3. Inadequate washing ...
... hands. It would NOT be because DNA from cells on your fingers hybridized to the probe. {Why? Even if the DNA in your cells was complementary to the probe, it is not available for hybridization as it is inside cells, and not denatured.} 3. Inadequate washing ...
DNA, Proteins, and Biotechnology
... State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the application of DNA profiling to determine paternity and also in forensic investigations. Analyse DNA ...
... State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the application of DNA profiling to determine paternity and also in forensic investigations. Analyse DNA ...
topic B - Institute of Life Sciences
... and protein sequences T Using searches based on homologous sequences, stored sequences suggest functions of newly identified genes and proteins ...
... and protein sequences T Using searches based on homologous sequences, stored sequences suggest functions of newly identified genes and proteins ...
Molecular Biology -
... amino acid sequence in a polypeptide which folds into a ____________ translation structure and function of the protein (e.g. normal hemoglobin vs. sickle cell hemoglobin) person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transc ...
... amino acid sequence in a polypeptide which folds into a ____________ translation structure and function of the protein (e.g. normal hemoglobin vs. sickle cell hemoglobin) person's characteristics or traits (e.g. normal health vs. sickle cell anemia) 2. The double helix structure of DNA, transc ...
Bio 301, Biochemistry I
... a. Primase catalyzes the de novo polymerization of DNA. b. RNA polymerase II initiates RNA synthesis by elongation of a DNA primer. c. During DNA replication, every Okazaki fragment synthesized by DNA polymerase III is elongated from a separate RNA primer. d. During DNA replication, leading strands ...
... a. Primase catalyzes the de novo polymerization of DNA. b. RNA polymerase II initiates RNA synthesis by elongation of a DNA primer. c. During DNA replication, every Okazaki fragment synthesized by DNA polymerase III is elongated from a separate RNA primer. d. During DNA replication, leading strands ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.