The chicken lysozyme chromatin domain contains a
... cultured cells and tissues with Trizol Reagent (Gibco BRL), according to the manufacturer’s instructions. RT–PCR A 5–10 µg sample of intact total RNA was treated with 1 U/µg RQ1 RNase-free DNase (Promega) in a total volume of 50–100 µl for 10 min at 37°C followed by heat inactivation of the enzyme a ...
... cultured cells and tissues with Trizol Reagent (Gibco BRL), according to the manufacturer’s instructions. RT–PCR A 5–10 µg sample of intact total RNA was treated with 1 U/µg RQ1 RNase-free DNase (Promega) in a total volume of 50–100 µl for 10 min at 37°C followed by heat inactivation of the enzyme a ...
Exam II Review Document
... Instead of the sigma regulatory protein we saw in bacteria, eukaryotes have many proteins that signal the start of transcription and help RNA polymerase bind to the promoter. In bacteria, related genes are regulated together via operons. In eukaryotes, related genes are not located next to each othe ...
... Instead of the sigma regulatory protein we saw in bacteria, eukaryotes have many proteins that signal the start of transcription and help RNA polymerase bind to the promoter. In bacteria, related genes are regulated together via operons. In eukaryotes, related genes are not located next to each othe ...
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... a) Discontinuous DNA synthesis of the lagging strand occurs in prokaryotes but not in eukaryotes. b) Single-stand binding protein and replication factor C (RFC) both bind to single-stranded DNA to prevent complementary base pairing. c) In both prokaryotes and eukaryotes only one type of DNA polymera ...
... a) Discontinuous DNA synthesis of the lagging strand occurs in prokaryotes but not in eukaryotes. b) Single-stand binding protein and replication factor C (RFC) both bind to single-stranded DNA to prevent complementary base pairing. c) In both prokaryotes and eukaryotes only one type of DNA polymera ...
Manipulating Genomes
... using resources from Cold Spring Harbor Laboratory. The GeneBoy tool on this site allows students to perform their own analysis of sections of sequence so they can understand how enormous sets of data are made sense of. The context of who should have access to DNA sequence data allows students to ex ...
... using resources from Cold Spring Harbor Laboratory. The GeneBoy tool on this site allows students to perform their own analysis of sections of sequence so they can understand how enormous sets of data are made sense of. The context of who should have access to DNA sequence data allows students to ex ...
DNA and RNA Replication
... 1. Observe the unwoven DNA molecule. One of the DNA strands is exposed, showing a sequence of nitrogen bases. 2. Click the Legend button for information about how nitrogen bases pair. 3. Build a mRNA molecule by pairing up free nitrogen bases in the nucleus with the nitrogen bases on the exposed str ...
... 1. Observe the unwoven DNA molecule. One of the DNA strands is exposed, showing a sequence of nitrogen bases. 2. Click the Legend button for information about how nitrogen bases pair. 3. Build a mRNA molecule by pairing up free nitrogen bases in the nucleus with the nitrogen bases on the exposed str ...
12.1 Components of Nucleic Acids
... The information carried on the mRNA will be used to produce proteins. The mRNA sequence is read three bases (triplet) at a time and each segment of three bases is called a codon. Each codon specifies a particular amino acid in the primary structure of the protein (its sequence of amino acids). There ...
... The information carried on the mRNA will be used to produce proteins. The mRNA sequence is read three bases (triplet) at a time and each segment of three bases is called a codon. Each codon specifies a particular amino acid in the primary structure of the protein (its sequence of amino acids). There ...
12_ Nucleic Acids
... The information carried on the mRNA will be used to produce proteins. The mRNA sequence is read three bases (triplet) at a time and each segment of three bases is called a codon. Each codon specifies a particular amino acid in the primary structure of the protein (its sequence of amino acids). There ...
... The information carried on the mRNA will be used to produce proteins. The mRNA sequence is read three bases (triplet) at a time and each segment of three bases is called a codon. Each codon specifies a particular amino acid in the primary structure of the protein (its sequence of amino acids). There ...
Introduction Kit components
... GF-1 AmbiClean Kit ( Gel & PCR ) contains special buffers to provide the correct salt concentration and pH for efficient recovery (80 - 90%) of DNA from both PCR product and agarose gel from both TAE or TBE buffer. This kit uses a specially treated glass filter membrane fixed into a column to effici ...
... GF-1 AmbiClean Kit ( Gel & PCR ) contains special buffers to provide the correct salt concentration and pH for efficient recovery (80 - 90%) of DNA from both PCR product and agarose gel from both TAE or TBE buffer. This kit uses a specially treated glass filter membrane fixed into a column to effici ...
effect of protein on gene expression
... expression differs in many aspects from that operating in single cell organism, and involves complex interactions of hormonal, neural and nutritional factors. ...
... expression differs in many aspects from that operating in single cell organism, and involves complex interactions of hormonal, neural and nutritional factors. ...
Promoter Regions
... base pairs before the transcription start site) Transcription Start Site: The beginning of RNA transcription. Downstream of binding sequences. Activator: A protein that binds DNA and stabilizes the binding of transcription factors. Activator Site: The region of DNA an activator binds to. Repressor: ...
... base pairs before the transcription start site) Transcription Start Site: The beginning of RNA transcription. Downstream of binding sequences. Activator: A protein that binds DNA and stabilizes the binding of transcription factors. Activator Site: The region of DNA an activator binds to. Repressor: ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.