SafeView - NBS Biologicals
... for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. SafeView is as se ...
... for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. SafeView is as se ...
How do proteins recognize DNA
... RNA polymerase traverses the template (antisense) strand, and following the rules of Watson-Crick complementarity with the antisense strand, creates an RNA copy of the sense (coding) strand. Polymerization is processive (without dissociation). Transcripts can be thousands or even millions of nucleot ...
... RNA polymerase traverses the template (antisense) strand, and following the rules of Watson-Crick complementarity with the antisense strand, creates an RNA copy of the sense (coding) strand. Polymerization is processive (without dissociation). Transcripts can be thousands or even millions of nucleot ...
Solutions for Practice Problems for Molecular Biology, Session 3
... f) A mutation occurs which results in the insertion of an extra G/C (top strand/bottom strand) base- pair immediately after base pair 11 (shown in bold). What effect will this insertion mutation have on the mRNA transcript and resulting protein? The mRNA will be longer by one nucleotide, but because ...
... f) A mutation occurs which results in the insertion of an extra G/C (top strand/bottom strand) base- pair immediately after base pair 11 (shown in bold). What effect will this insertion mutation have on the mRNA transcript and resulting protein? The mRNA will be longer by one nucleotide, but because ...
DNA Review Worksheet
... complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the same amino acid so the process can occur again and again. 5. Th ...
... complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the same amino acid so the process can occur again and again. 5. Th ...
I. DNA, Chromosomes, Chromatin, and Genes II. DNA
... complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the same amino acid so the process can occur again and again. 5. Th ...
... complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a peptide bond. As process continues, amino acid chain is formed until a stop codon. 4. The tRNA is recycled to find another of the same amino acid so the process can occur again and again. 5. Th ...
Gene Expression
... thymine (T) cytosine (C) uracil (U) pentose deoxyribose ribose phosphate nucleotide complementary bases DNA RNA ...
... thymine (T) cytosine (C) uracil (U) pentose deoxyribose ribose phosphate nucleotide complementary bases DNA RNA ...
Genetic Transformation computer exercise
... mutated (GeneB) genes; this is known as a DNA sequence alignment. An alignment uses an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those ...
... mutated (GeneB) genes; this is known as a DNA sequence alignment. An alignment uses an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those ...
molecular biology
... endonuclease will digest a nucleic acid molecule any where in the middle of the chain. As a result the oligonucleotides are formed. The smallest molecule that can be formed as the action of an endonuclease is a dinucleodtide. An exonuclease, on the other hand, digests the nucleic acid either from it ...
... endonuclease will digest a nucleic acid molecule any where in the middle of the chain. As a result the oligonucleotides are formed. The smallest molecule that can be formed as the action of an endonuclease is a dinucleodtide. An exonuclease, on the other hand, digests the nucleic acid either from it ...
DNA Replication
... fMet-tRNA complex will be bound, is distinguished from other similar codons by its proximity to the Shine-Dalgarno sequence. ...
... fMet-tRNA complex will be bound, is distinguished from other similar codons by its proximity to the Shine-Dalgarno sequence. ...
Supplementary Methods
... background fluorescence. Spine volume was measured in principal as reported previously, assuming free diffusion of eGFP throughout the dendritic tree 40. Briefly, regions of interest (ROIs) were drawn around spines in the projection image and nearby dendrites. The average fluorescence of pixels with ...
... background fluorescence. Spine volume was measured in principal as reported previously, assuming free diffusion of eGFP throughout the dendritic tree 40. Briefly, regions of interest (ROIs) were drawn around spines in the projection image and nearby dendrites. The average fluorescence of pixels with ...
Gene Cloning, Expression, and Substrate Specificity of an Imidase
... [pH 8.0] 500 mM NaCl, and 50 mM imidazole). Then the target protein was eluted out with elution buffer (50 mM Tris-HCl [pH 8.0] containing 500 mM NaCl and 1 M imidazole). The enzyme was collected and stored at 4C for further analysis. Assay of imidase activity. Imidase activity was measured by a sp ...
... [pH 8.0] 500 mM NaCl, and 50 mM imidazole). Then the target protein was eluted out with elution buffer (50 mM Tris-HCl [pH 8.0] containing 500 mM NaCl and 1 M imidazole). The enzyme was collected and stored at 4C for further analysis. Assay of imidase activity. Imidase activity was measured by a sp ...
Role of Deoxyribonucleic Acid Polymerase beta in Nuclear
... herpes simplex virus, DNA of both types has been extensively used. Several useful properties of the purified, virus-induced enzymes have been exploited in order to gain a better understanding of the nature of ‘activated’ DNA. In order to decrease the number of experimental variables, the DNA was ren ...
... herpes simplex virus, DNA of both types has been extensively used. Several useful properties of the purified, virus-induced enzymes have been exploited in order to gain a better understanding of the nature of ‘activated’ DNA. In order to decrease the number of experimental variables, the DNA was ren ...
DNA and Forensic Science
... initially chosen for analysis. This cycle of heating and cooling is then repeated many times until an exponential rate of replication occurs and a very large amount of DNA containing the specific region is obtained. This enables the investigators to further manipulate or analyze this region of DNA w ...
... initially chosen for analysis. This cycle of heating and cooling is then repeated many times until an exponential rate of replication occurs and a very large amount of DNA containing the specific region is obtained. This enables the investigators to further manipulate or analyze this region of DNA w ...
VWR Taq DNA Polymerase Master Mix
... 65 °C for 20 – 40 seconds allowing annealing of the primers to the single-stranded DNA template. Typically, the annealing temperature is about 3 – 5 °C below the Tm of the primers used. Extension/elongation step: Taq polymerase has its optimal activity at 72 °C. At this step the DNA polymerase synth ...
... 65 °C for 20 – 40 seconds allowing annealing of the primers to the single-stranded DNA template. Typically, the annealing temperature is about 3 – 5 °C below the Tm of the primers used. Extension/elongation step: Taq polymerase has its optimal activity at 72 °C. At this step the DNA polymerase synth ...
DNA Testing of Seafood: Technical Guide
... There are many scientific uses for these techniques. For example Bui et al [11] used DNA methods to determine the species of eggs in plankton samples, in order to map spawning grounds of cod off eastern Canada. Fish stocks are assessed in given geographical areas described as ‘management stocks’. ...
... There are many scientific uses for these techniques. For example Bui et al [11] used DNA methods to determine the species of eggs in plankton samples, in order to map spawning grounds of cod off eastern Canada. Fish stocks are assessed in given geographical areas described as ‘management stocks’. ...
Cloning and Expression Characteristics of the Pig Stra8 Gene
... female. The timing of meiosis entry differs significantly between male and female germ cells. At nearly 13.5 days post-coitum (dpc), female primordial germ cells (PGCs) initiate meiotic process and then arrest at the dictyate stage, whereas male PGCs arrest at the G0/G1 stage of the cell cycle in th ...
... female. The timing of meiosis entry differs significantly between male and female germ cells. At nearly 13.5 days post-coitum (dpc), female primordial germ cells (PGCs) initiate meiotic process and then arrest at the dictyate stage, whereas male PGCs arrest at the G0/G1 stage of the cell cycle in th ...
Cloning and Expression Characteristics of the Pig Stra8 Gene
... female. The timing of meiosis entry differs significantly between male and female germ cells. At nearly 13.5 days post-coitum (dpc), female primordial germ cells (PGCs) initiate meiotic process and then arrest at the dictyate stage, whereas male PGCs arrest at the G0/G1 stage of the cell cycle in th ...
... female. The timing of meiosis entry differs significantly between male and female germ cells. At nearly 13.5 days post-coitum (dpc), female primordial germ cells (PGCs) initiate meiotic process and then arrest at the dictyate stage, whereas male PGCs arrest at the G0/G1 stage of the cell cycle in th ...
20_Lecture_Presentation
... cloned eukaryotic genes in bacterial host cells Eukaryotic genome extremely large, so using a cDNA would be better because only has the exons To overcome differences in promoters and other DNA ...
... cloned eukaryotic genes in bacterial host cells Eukaryotic genome extremely large, so using a cDNA would be better because only has the exons To overcome differences in promoters and other DNA ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.