DNA -> RNA -> Proteins
... of DNA that codes for a specific protein • Only a very small percentage of our DNA (perhaps 1%) actually does this ...
... of DNA that codes for a specific protein • Only a very small percentage of our DNA (perhaps 1%) actually does this ...
sharpmass™ 50
... SHARPMASS™50 Ready-to-load DNA Ladder consists of 17 DNA fragments ranging from 50 bp to 1.5 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. T ...
... SHARPMASS™50 Ready-to-load DNA Ladder consists of 17 DNA fragments ranging from 50 bp to 1.5 kb. It is designed to show virtually uniform spacing over a wide fragment range. The ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. T ...
MBLG2x71 Course Information for mmb web site
... these processes can be studied and manipulated in the laboratory. Experiments in model organisms are provided to illustrate how the field has advanced, together with discussion of work carried out in human systems and the relevance to human genetic diseases. The tools of molecular biology are taught ...
... these processes can be studied and manipulated in the laboratory. Experiments in model organisms are provided to illustrate how the field has advanced, together with discussion of work carried out in human systems and the relevance to human genetic diseases. The tools of molecular biology are taught ...
MGB_LNA_Substitutes
... hairpin region increase its melting temperature by 4.5°C. It is important to note that the effective increase of melting temperature per single nucleotide exchange is subject to variation. The main parameters are the position of the respective base to be substituted as well as the whole sequence. ...
... hairpin region increase its melting temperature by 4.5°C. It is important to note that the effective increase of melting temperature per single nucleotide exchange is subject to variation. The main parameters are the position of the respective base to be substituted as well as the whole sequence. ...
from innovative technologies ...to superior key products
... Nucleic acids store and transfer genetic information in cells. The main types of nucleic acids are DNA and R NA, which are made up of chains of chemicals called nucleotides. Most DNA exists in cells as a double-stranded structure that resembles a twisted ladder. The nucleotides on opposite sides of ...
... Nucleic acids store and transfer genetic information in cells. The main types of nucleic acids are DNA and R NA, which are made up of chains of chemicals called nucleotides. Most DNA exists in cells as a double-stranded structure that resembles a twisted ladder. The nucleotides on opposite sides of ...
January 7, 2014 Notes Transcription: process of copying DNA into
... January 7, 2014 Notes Transcription: process of copying DNA into an RNA template. (Occurs in nucleus) ...
... January 7, 2014 Notes Transcription: process of copying DNA into an RNA template. (Occurs in nucleus) ...
ChIP-seq
... Use PCR to amplify specific DNA sequences to see if they were precipitated with the antibody. ...
... Use PCR to amplify specific DNA sequences to see if they were precipitated with the antibody. ...
BACKGROUND: UvrC is a DNA repair enzyme found in all
... BACKGROUND: UvrC is a DNA repair enzyme found in all prokaryotes and its critical in maintaining DNA integrity. What You Need to Know: NCBI Protein Blast FASTA format Blastp Other sequence alignment tools… YOUR JOB: A. Find an amino acid sequence of UvrC from five different prokaryotic species (one ...
... BACKGROUND: UvrC is a DNA repair enzyme found in all prokaryotes and its critical in maintaining DNA integrity. What You Need to Know: NCBI Protein Blast FASTA format Blastp Other sequence alignment tools… YOUR JOB: A. Find an amino acid sequence of UvrC from five different prokaryotic species (one ...
Chapter 20
... Insert synthetic double stranded RNA’s that match a gene that will inactivate translation This was used to identify the function of C. elegans genes ...
... Insert synthetic double stranded RNA’s that match a gene that will inactivate translation This was used to identify the function of C. elegans genes ...
CALF THYMUS DNA, ACTIVATED - Sigma
... of α- P-TTP (3000 Ci/mmol); and 20 units of DNA Polymerase (Sigma Catalog No. D 9380). 39% of the ...
... of α- P-TTP (3000 Ci/mmol); and 20 units of DNA Polymerase (Sigma Catalog No. D 9380). 39% of the ...
Reverse Transcription - St. Michael`s Hospital
... Synthesis of cDNA from purified poly(A)+ or total RNA is performed by the action of a reverse transcriptase, typically isolated from retrovirus. The reverse transcriptase has three biochemical activities: as a RNA‐dependent DNA polymerase, a DNA‐dependent DNA polymerase and ribonuclease H. Many c ...
... Synthesis of cDNA from purified poly(A)+ or total RNA is performed by the action of a reverse transcriptase, typically isolated from retrovirus. The reverse transcriptase has three biochemical activities: as a RNA‐dependent DNA polymerase, a DNA‐dependent DNA polymerase and ribonuclease H. Many c ...
Name
... A gene of interest is identified. The plasmid and gene of interest are both cut with the same restriction enzyme. The gene is then inserted into the bacteria and DNA ligase binds the two fragments together ...
... A gene of interest is identified. The plasmid and gene of interest are both cut with the same restriction enzyme. The gene is then inserted into the bacteria and DNA ligase binds the two fragments together ...
Target-triggered DNA three-way junction superstructure and
... Subtyping of influenza is critical for its treatment, diagnosis and surveillance. Due to the emergence of new subtypes and highly pathogenic avian influenza viruses, novel analytical approaches for monitoring their appearance and further facilitating the development of vaccine are in urgent need [1] ...
... Subtyping of influenza is critical for its treatment, diagnosis and surveillance. Due to the emergence of new subtypes and highly pathogenic avian influenza viruses, novel analytical approaches for monitoring their appearance and further facilitating the development of vaccine are in urgent need [1] ...
Study guide
... Strands of nucleotides held together by sugar-phosphate backbone. Two strands are paired together with hydrogen bonds between paired bases. One strand is the template for the other (base pairing rules— this property gives DNA its unique quality of being able to self-replicate) DNA replication DNA tr ...
... Strands of nucleotides held together by sugar-phosphate backbone. Two strands are paired together with hydrogen bonds between paired bases. One strand is the template for the other (base pairing rules— this property gives DNA its unique quality of being able to self-replicate) DNA replication DNA tr ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.