BIOTRANSFORMATION OF DRUGS
... or unstable. From an unstable hydroxylated metabolite a group may break off spontaneously: an alkyl group, ammonia, a halogen atom, or sulfur atom; such reactions are called oxidative dealkylation, oxidative deamination, oxidative dehalogenation, and oxidative desulfuration, respectively. CYP can al ...
... or unstable. From an unstable hydroxylated metabolite a group may break off spontaneously: an alkyl group, ammonia, a halogen atom, or sulfur atom; such reactions are called oxidative dealkylation, oxidative deamination, oxidative dehalogenation, and oxidative desulfuration, respectively. CYP can al ...
Mechanistic studies on the diazo transfer reaction
... methods have been developed for introducing an azide group.8,9 The most important of these is the ‘diazo’ transfer reaction, where a diazo group (N2) is transferred to an amine to form an azide.10,11 In this reaction, the stereochemistry of the starting material is retained and there are no side pr ...
... methods have been developed for introducing an azide group.8,9 The most important of these is the ‘diazo’ transfer reaction, where a diazo group (N2) is transferred to an amine to form an azide.10,11 In this reaction, the stereochemistry of the starting material is retained and there are no side pr ...
Branched-chain amino acid restriction in Zucker
... in the intrascapular region. Buprenorphine (0.03 mg/kg, s.c.) was postsurgically administered for pain control, and animals recovered to preoperative weight prior to experiments. Buprenorphine was given to all animals in the first 3 days following surgery, and then removed for 4 days until clamps wer ...
... in the intrascapular region. Buprenorphine (0.03 mg/kg, s.c.) was postsurgically administered for pain control, and animals recovered to preoperative weight prior to experiments. Buprenorphine was given to all animals in the first 3 days following surgery, and then removed for 4 days until clamps wer ...
Developement of strategies for the isotopic labeling of methyl
... For optimized combinatorial labeling, the choice of the amino acids to label simultaneously and the precursors as well as the protocol for their incorporation has to be carefully studied. In this work, a new protocol was introduced for the scrambling-free and optimized isotopic labeling of AILV) ...
... For optimized combinatorial labeling, the choice of the amino acids to label simultaneously and the precursors as well as the protocol for their incorporation has to be carefully studied. In this work, a new protocol was introduced for the scrambling-free and optimized isotopic labeling of AILV) ...
Lab Handbook 1_5 - Central Manchester University Hospitals
... The Unit is housed in a purpose-built building completed in 1985. Ground floor accommodation includes the clinic area and office suite. The first floor contains the laboratories responsible for the Region's newborn screening programme as well as a wide range of biochemical and molecular investigatio ...
... The Unit is housed in a purpose-built building completed in 1985. Ground floor accommodation includes the clinic area and office suite. The first floor contains the laboratories responsible for the Region's newborn screening programme as well as a wide range of biochemical and molecular investigatio ...
Biomarker discovery for psychiatric disorders
... ________________________________________________________________________ Within the spectrum of anxiety disorders, the facts that they often co-occur (Hettema et al., 2005), and most of them respond to a similar spectrum of pharmacological treatments (Gross and Hen, 2004; Kent et al., 2002) indicate ...
... ________________________________________________________________________ Within the spectrum of anxiety disorders, the facts that they often co-occur (Hettema et al., 2005), and most of them respond to a similar spectrum of pharmacological treatments (Gross and Hen, 2004; Kent et al., 2002) indicate ...
Nature inspired platforms for production of acetyl
... some microorganisms can produce certain biofuels naturally6,7, native microorganisms often suffer from low growth rates, intolerance of toxic biofuel products, and incomplete carbon source usage (i.e. many microorganisms cannot metabolize xylose, which comprises approximately 30% of plant cellulosic ...
... some microorganisms can produce certain biofuels naturally6,7, native microorganisms often suffer from low growth rates, intolerance of toxic biofuel products, and incomplete carbon source usage (i.e. many microorganisms cannot metabolize xylose, which comprises approximately 30% of plant cellulosic ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) ISSN: 2278-3008.
... Determine the content of Desmopressin in pharmaceutical preparation by the biological method based on work of brsges and Thoron (5,6,7) Biological activity of Desmopressin estimated by measuring the anti – diuretic effect of studied preparation compare with standard Desmopressin . The possibility of ...
... Determine the content of Desmopressin in pharmaceutical preparation by the biological method based on work of brsges and Thoron (5,6,7) Biological activity of Desmopressin estimated by measuring the anti – diuretic effect of studied preparation compare with standard Desmopressin . The possibility of ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... these ‘omics’ techniques is enormous and therefore the early ‘omics’ papers focused more on data acquisition than on answering biological questions [282]. The current challenge is to analyze and interpret the resulting large, complex datasets. To achieve this, various approaches are currently taken, ...
... these ‘omics’ techniques is enormous and therefore the early ‘omics’ papers focused more on data acquisition than on answering biological questions [282]. The current challenge is to analyze and interpret the resulting large, complex datasets. To achieve this, various approaches are currently taken, ...
Intermediary metabolism
... 2. Major intermediary metabolic pathways in C. elegans 2.1. Metabolic studies in related species Recent estimates for the last common ancestor to vertebrates and nematodes range from 800 million to over one billion years ago (Hedges and Kumar, 2003; Hedges, 2002; Ureta-Vidal et al., 2003; Blaxter, 1 ...
... 2. Major intermediary metabolic pathways in C. elegans 2.1. Metabolic studies in related species Recent estimates for the last common ancestor to vertebrates and nematodes range from 800 million to over one billion years ago (Hedges and Kumar, 2003; Hedges, 2002; Ureta-Vidal et al., 2003; Blaxter, 1 ...
Protein Sequencing and Identification With Mass
... • A method that cross-correlates related spectra (e.g., from normal and diseased individuals) would be valuable in functional proteomics. ...
... • A method that cross-correlates related spectra (e.g., from normal and diseased individuals) would be valuable in functional proteomics. ...
Biochemistry of exercise-induced metabolic acidosis
... intermolecular esters, forming polylactate structures such as the two molecular lactoyllactic acid. Nevertheless, the discovery that lactic acid could crystallize occurred as early as 1895 (17). Subsequent work on quantifying the physical properties of lactic acid were complicated by the difficultie ...
... intermolecular esters, forming polylactate structures such as the two molecular lactoyllactic acid. Nevertheless, the discovery that lactic acid could crystallize occurred as early as 1895 (17). Subsequent work on quantifying the physical properties of lactic acid were complicated by the difficultie ...
Sustained nonoxidative glucose utilization and depletion of
... The coefficient of variation for specific activity of lactate was 2.5% in our laboratory (six analyses of one sample). The 14C0, was collected directly from blood by a previously established diffusion method having a 2.9% coefficient of variation (14,16). L-[U-‘3C]lactate analysis. L-[U-‘3C] lactate ...
... The coefficient of variation for specific activity of lactate was 2.5% in our laboratory (six analyses of one sample). The 14C0, was collected directly from blood by a previously established diffusion method having a 2.9% coefficient of variation (14,16). L-[U-‘3C]lactate analysis. L-[U-‘3C] lactate ...
biochem hnd final - Yaba College of Technology
... in the SIWES is an indication that the student has not shown sufficient interest in the field or has no potential to become a skilled technician in his field. The SIWES should be graded on a fail or pass basis. Where a student has satisfied all other requirements but failed SIWES, he may only be all ...
... in the SIWES is an indication that the student has not shown sufficient interest in the field or has no potential to become a skilled technician in his field. The SIWES should be graded on a fail or pass basis. Where a student has satisfied all other requirements but failed SIWES, he may only be all ...
David Rittenberg - National Academy of Sciences
... was instead assigned to H. T. Clarke, chair of the Department of Biochemistry at P&S to promote biological uses of 2H. At that time one interest of Clarke’s was the possibility of demonstrating optical activity for a compound, Cab1H2H. Clarke also suggested to Rittenberg a “roving commission” to tal ...
... was instead assigned to H. T. Clarke, chair of the Department of Biochemistry at P&S to promote biological uses of 2H. At that time one interest of Clarke’s was the possibility of demonstrating optical activity for a compound, Cab1H2H. Clarke also suggested to Rittenberg a “roving commission” to tal ...
Short-Chain-Length Polyhydroxyalkanoates: Synthesis in
... In this process, propionic acid was added to supply the precursors for 3HV monomer (Fig. 2). The same strategy was applied to produce P(3HB-co-3HV) by recombinant E. coli. Because the assimilation of propionic acid in normal E. coli strain is rather inefficient, the fadR atoC mutant E. coli LS5218, ...
... In this process, propionic acid was added to supply the precursors for 3HV monomer (Fig. 2). The same strategy was applied to produce P(3HB-co-3HV) by recombinant E. coli. Because the assimilation of propionic acid in normal E. coli strain is rather inefficient, the fadR atoC mutant E. coli LS5218, ...
Retention of the Cis Proline Conformation in Tripeptide Fragments of
... protecting groups and purification of Ac-N-dmP-Y-OH were accomplished as described above with a yield of 58% (6 mg, 0.013 mmol). The two tripeptides were characterized by analytical HPLC, capillary electrophoresis (CE), and matrix-assisted laser desorption/chemical ionization mass spectrometry (MALD ...
... protecting groups and purification of Ac-N-dmP-Y-OH were accomplished as described above with a yield of 58% (6 mg, 0.013 mmol). The two tripeptides were characterized by analytical HPLC, capillary electrophoresis (CE), and matrix-assisted laser desorption/chemical ionization mass spectrometry (MALD ...
microbe co-metabolic traits in the gut of Gorilla spp
... and McNeilage, 1998). Fruit tends to have more easily digestible energy than vegetation, which is typically higher in less digestible structural carbohydrates such as hemicellulose, cellulose and lignin (Chapman et al., 2012). Thus, given the role of gut microbes in providing access to otherwise ind ...
... and McNeilage, 1998). Fruit tends to have more easily digestible energy than vegetation, which is typically higher in less digestible structural carbohydrates such as hemicellulose, cellulose and lignin (Chapman et al., 2012). Thus, given the role of gut microbes in providing access to otherwise ind ...
understanding carbon metabolism in hydrogen production
... Table H.1: Effect of initial glutamate flux in the case of maximum biomass growth rate (Raw data of Figure 4.3-4.8) .............................................................................159 Table H.2: Effect of initial glutamate flux in the case of maximum hydrogen production rate (Raw data o ...
... Table H.1: Effect of initial glutamate flux in the case of maximum biomass growth rate (Raw data of Figure 4.3-4.8) .............................................................................159 Table H.2: Effect of initial glutamate flux in the case of maximum hydrogen production rate (Raw data o ...
Carbon–hydrogen bond activation of chloroalkanes by a rhodium
... coordinatively unsaturated metal complexes is a wellstudied area of organometallic chemistry [1]. A number of complexes shown to be successful in alkane activation are Cp or Cp* metal complexes [2–4]. More recently, however, the analogous trispyrazolylborate complexes [5] have been shown to also act ...
... coordinatively unsaturated metal complexes is a wellstudied area of organometallic chemistry [1]. A number of complexes shown to be successful in alkane activation are Cp or Cp* metal complexes [2–4]. More recently, however, the analogous trispyrazolylborate complexes [5] have been shown to also act ...
The Impact of Ligand Design on the Coordination Chemistry and
... donors. Ever since initial reports of transition metal pincer complexes were published in the late 1970’s, there has been burgeoning interest in such complexes because of their desirable robust nature, generally simple syntheses, and the spectacular chemical transformations that they can mediate. In ...
... donors. Ever since initial reports of transition metal pincer complexes were published in the late 1970’s, there has been burgeoning interest in such complexes because of their desirable robust nature, generally simple syntheses, and the spectacular chemical transformations that they can mediate. In ...
ATP utilization associated with recovery metabolism in - AJP-Cell
... partial uncoupling of oxidative phosphorylation in the muscle mitochondria (7, 13, 36). The experiments described in this paper were designed to find out if there was significant suprabasal ATP breakdown in frog sartorii during the metabolic restoration of ATP utilized during prior contraction. Our ...
... partial uncoupling of oxidative phosphorylation in the muscle mitochondria (7, 13, 36). The experiments described in this paper were designed to find out if there was significant suprabasal ATP breakdown in frog sartorii during the metabolic restoration of ATP utilized during prior contraction. Our ...
Lecture 1 - Imperial College London
... NB. lysine (Lys) is proteinogenic whereas ornithine (Orn) is not phenylalanine (Phe), tyrosine (Tyr) & tryptophan (Trp) from shikimate are the other important precursors biosynthesis is via reductive amination of the appropriate -ketoacid mediated by pyridoxal-5’-phosphate ...
... NB. lysine (Lys) is proteinogenic whereas ornithine (Orn) is not phenylalanine (Phe), tyrosine (Tyr) & tryptophan (Trp) from shikimate are the other important precursors biosynthesis is via reductive amination of the appropriate -ketoacid mediated by pyridoxal-5’-phosphate ...
Engineering of polyketide biosynthetic pathways for bioactive
... career. Without his instructions, it would have been impossible for me to get so much work done in four years and finish this dissertation. I would also like to thank my committee members, Dr. Randy Lewis, Dr. David Britt, Dr. Dong Chen and Dr. Foster Agblevor for their advice and feedback, which he ...
... career. Without his instructions, it would have been impossible for me to get so much work done in four years and finish this dissertation. I would also like to thank my committee members, Dr. Randy Lewis, Dr. David Britt, Dr. Dong Chen and Dr. Foster Agblevor for their advice and feedback, which he ...
Propionate stimulates pyruvate oxidation in the - AJP
... derived from HP [1-13C]pyruvate has been proposed as a method for identifying viable myocardium. The efficacy of propionate for increasing PDH flux in the setting of PDH inhibition by an alternative substrate was studied using isotopomer analysis paired with exams using HP [1-13C]pyruvate. Hearts fr ...
... derived from HP [1-13C]pyruvate has been proposed as a method for identifying viable myocardium. The efficacy of propionate for increasing PDH flux in the setting of PDH inhibition by an alternative substrate was studied using isotopomer analysis paired with exams using HP [1-13C]pyruvate. Hearts fr ...