Hybridoma Technology
... • Fusion of spleen cells to myeloma cells is induced using polyethylene glycol (PEG), to produce hybridoma • Hybridomas are grown in selective hypoxanthine aminopterin thymidine (HAT) medium. • HAT medium contains a drug, aminopterin that blocks one pathway for nucleotide synthesis, making the cells ...
... • Fusion of spleen cells to myeloma cells is induced using polyethylene glycol (PEG), to produce hybridoma • Hybridomas are grown in selective hypoxanthine aminopterin thymidine (HAT) medium. • HAT medium contains a drug, aminopterin that blocks one pathway for nucleotide synthesis, making the cells ...
Plasma membrane
... ▫ Pumps: actively pump specific molecules across the membrane Sodium potassium pump: a method of active transport that allows cells to have a high concentration of potassium but low concentrations of sodium. This imbalance is how nerve cells are able to transmit impulses and respond to stimuli. ...
... ▫ Pumps: actively pump specific molecules across the membrane Sodium potassium pump: a method of active transport that allows cells to have a high concentration of potassium but low concentrations of sodium. This imbalance is how nerve cells are able to transmit impulses and respond to stimuli. ...
doc - General Biology
... encloses a thick fluid called the matrix. Mitochondria and chloroplasts contain their own DNA, which encodes some of their proteins. This DNA is evidence that mitochondria and chloroplasts evolved from free-living prokaryotes in the distant past. ...
... encloses a thick fluid called the matrix. Mitochondria and chloroplasts contain their own DNA, which encodes some of their proteins. This DNA is evidence that mitochondria and chloroplasts evolved from free-living prokaryotes in the distant past. ...
Membrane structure, I
... Osmoregulation~ control in cells with cell walls: Turgid (very firm) - Hypo Flaccid (limp) - Iso Plasmolysis~ Hyper ...
... Osmoregulation~ control in cells with cell walls: Turgid (very firm) - Hypo Flaccid (limp) - Iso Plasmolysis~ Hyper ...
topic-3.doc
... Spore stain: used to stain endospores of Clostridium and Bacillus o endospores do not readily take up dye, but once it penetrates the stain is not easily decolorized o heat smear over steam, rinse with water o counterstain with safranin o Endospores - Green; Vegetative cells - Red Flagellar stain: f ...
... Spore stain: used to stain endospores of Clostridium and Bacillus o endospores do not readily take up dye, but once it penetrates the stain is not easily decolorized o heat smear over steam, rinse with water o counterstain with safranin o Endospores - Green; Vegetative cells - Red Flagellar stain: f ...
Chapter 15 Regulation of Cell Number Normal and Cancer Cells
... The engines that drive progression from one step of the cell cycle to the next are a series of protein complexes composed of two subunits: a cyclin and a cyclin-dependent protein kinase (abbreviated CDK). In every eukaryote, there is a family of structurally and functionally related cyclin proteins. ...
... The engines that drive progression from one step of the cell cycle to the next are a series of protein complexes composed of two subunits: a cyclin and a cyclin-dependent protein kinase (abbreviated CDK). In every eukaryote, there is a family of structurally and functionally related cyclin proteins. ...
File
... How it works: A protein skeleton beneath the cell membrane can be assembled to push and pull the membrane into new shapes. What it’s made of: proteins on the surface of the cell Where it is found: cell membrane surface When it is used: when a cell needs to push against a fluid Why/ its function: to ...
... How it works: A protein skeleton beneath the cell membrane can be assembled to push and pull the membrane into new shapes. What it’s made of: proteins on the surface of the cell Where it is found: cell membrane surface When it is used: when a cell needs to push against a fluid Why/ its function: to ...
2. ______ Active Transport uses the energy
... B. ____________ (molecular) Motion – the random motion of microscopic particles in a solid, liquid or gas, caused by ____________ with surrounding molecules – is the basis for molecular movement C. Two ____________ of a molecule influence its movement across a cell membrane – size and polarity 1. __ ...
... B. ____________ (molecular) Motion – the random motion of microscopic particles in a solid, liquid or gas, caused by ____________ with surrounding molecules – is the basis for molecular movement C. Two ____________ of a molecule influence its movement across a cell membrane – size and polarity 1. __ ...
![[PLANT CELL WALL] Functions of Cell Wall Structure of Cell Wall](http://s1.studyres.com/store/data/014512284_1-fafd2bca61d6dff1e76fb2585a0a6724-300x300.png)
[PLANT CELL WALL] Functions of Cell Wall Structure of Cell Wall
... Functions of Cell Wall Maintaining and determining cell shape. Provide mechanical support and allows the plants to grow tall. Prevents the cell membrane rupture due to water pressure (turgor pressure). Provide mechanical protection from insects and pathogens. Biochemical activity in wall c ...
... Functions of Cell Wall Maintaining and determining cell shape. Provide mechanical support and allows the plants to grow tall. Prevents the cell membrane rupture due to water pressure (turgor pressure). Provide mechanical protection from insects and pathogens. Biochemical activity in wall c ...
CH 7 CQ
... two layers of phospholipids (with opposite orientations of the phospholipids in each layer) with each layer covered on the outside with proteins ...
... two layers of phospholipids (with opposite orientations of the phospholipids in each layer) with each layer covered on the outside with proteins ...
PPT
... Tonicity is a relative term • Hypotonic Solution - One solution has a lower concentration of solute than another. • Hypertonic Solution - one solution has a higher concentration of solute than another. • Isotonic Solution - both solutions have same concentrations of solute. ...
... Tonicity is a relative term • Hypotonic Solution - One solution has a lower concentration of solute than another. • Hypertonic Solution - one solution has a higher concentration of solute than another. • Isotonic Solution - both solutions have same concentrations of solute. ...
Protists Fungi Plants
... • Fungus-like protists: sporozoans—immobile, heterotrophic, reproduce sexually in 1 host and then asexually in the next, parasitic ...
... • Fungus-like protists: sporozoans—immobile, heterotrophic, reproduce sexually in 1 host and then asexually in the next, parasitic ...
Science 8 Questions 1. What does Organelle mean? 2. What is
... 19. What is the job of cilia and flagella? 20. What is the difference between cilia and flagella? 21. What cells have a cell wall? 22. What is the job of the cell wall? 23. What is the job of chloroplast? 24. What is the job of the vacuole? 25. How do plant and animal cells differ in regards to vacu ...
... 19. What is the job of cilia and flagella? 20. What is the difference between cilia and flagella? 21. What cells have a cell wall? 22. What is the job of the cell wall? 23. What is the job of chloroplast? 24. What is the job of the vacuole? 25. How do plant and animal cells differ in regards to vacu ...
Unit 2: Cell Biology Study Guide
... 17. A fertilized egg receives One set(s) of chromosomes from EACH parent. 18. An offspring inherits 50 % DNA from its mother and 50 % from its father. 19. A trait that requires two of the same allele to be observed is called recessive. 20. A trait that requires only one allele to be observed is call ...
... 17. A fertilized egg receives One set(s) of chromosomes from EACH parent. 18. An offspring inherits 50 % DNA from its mother and 50 % from its father. 19. A trait that requires two of the same allele to be observed is called recessive. 20. A trait that requires only one allele to be observed is call ...
Cell Reproduction: Mitosis & Meiosis
... Cytokinesis in Animal Cells Contractile ring mechanism Halfway between cell’s poles, plasma membrane constricts = cleavage furrow (ATP energy causes contraction of actin filaments) ...
... Cytokinesis in Animal Cells Contractile ring mechanism Halfway between cell’s poles, plasma membrane constricts = cleavage furrow (ATP energy causes contraction of actin filaments) ...
Characteristic #4
... o Metaphase: The chromosomes line up on the cell equator, and the fibers attach to them from both sides o Anaphase: The fibers shorten, splitting the chromosomes in half and pulling one half to each pole o Telophase: A new nucleus begins to form around each set of chromosomes, and the cell starts to ...
... o Metaphase: The chromosomes line up on the cell equator, and the fibers attach to them from both sides o Anaphase: The fibers shorten, splitting the chromosomes in half and pulling one half to each pole o Telophase: A new nucleus begins to form around each set of chromosomes, and the cell starts to ...
Cell Division - Mitosis Lecture PowerPoint
... 3. Cell elongates; new plasma membrane and cell wall are added between chromosomes, pushing them towards opposite ends of cell. 4. Parent cell is divided into two identical daughter cells. From the Virtual Cell Biology Classroom on ScienceProfOnline.com ...
... 3. Cell elongates; new plasma membrane and cell wall are added between chromosomes, pushing them towards opposite ends of cell. 4. Parent cell is divided into two identical daughter cells. From the Virtual Cell Biology Classroom on ScienceProfOnline.com ...
Estimating the Time Needed for Mitosis
... In this lab, you will determine the approximate time it takes for plant and animal cells to pass through each of the four stages of mitosis. You will do this by counting the number of onion root tip cells and whitefish blastula cells in each of the four phases of mitosis and in interphase. Many cell ...
... In this lab, you will determine the approximate time it takes for plant and animal cells to pass through each of the four stages of mitosis. You will do this by counting the number of onion root tip cells and whitefish blastula cells in each of the four phases of mitosis and in interphase. Many cell ...
Chapter 19 - Cloudfront.net
... Plasmid DNA replication starts. The free DNA strand starts moving through tube In the recipient cell, replication starts on the transferred DNA The cells move apart and the plasmid in each forms a ...
... Plasmid DNA replication starts. The free DNA strand starts moving through tube In the recipient cell, replication starts on the transferred DNA The cells move apart and the plasmid in each forms a ...
S0735109709025054_mmc1
... registered CT studies, thus enabling accurate measurement of the percentage of the net injected dose (%ID) retained intramyocardially in vivo (%ID=100 * [Activity within VOI / (Activity in syringe before injection-Activity in syringe after injection)]. Quantification of engraftment by real time PCR ...
... registered CT studies, thus enabling accurate measurement of the percentage of the net injected dose (%ID) retained intramyocardially in vivo (%ID=100 * [Activity within VOI / (Activity in syringe before injection-Activity in syringe after injection)]. Quantification of engraftment by real time PCR ...
MICROBIOLOGY LECTURE TITLE: Measuring Bacterial Growth
... Serial Dilutions Work ___________ using multiplication with the “___________ ____________” ________ ________ - the ______ ___ ______ that you have diluted the bacteria sample with the diluent solution Make a determination of the numbers of bacteria in ______ ________ _________. ...
... Serial Dilutions Work ___________ using multiplication with the “___________ ____________” ________ ________ - the ______ ___ ______ that you have diluted the bacteria sample with the diluent solution Make a determination of the numbers of bacteria in ______ ________ _________. ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.