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General western blot protocol
General western blot protocol

... μL of ice cold lysis buffer rapidly to the tube, homogenize with an electric homogenizer, rinse the blade twice with another 2 x 200 μL lysis buffer, then maintain constant agitation for 2 h at 4°C (eg place on an orbital shaker in the fridge). Volumes of lysis buffer must be determined in relation ...
PROPERTY OF: BIOLOGY – UNIT 3 – CHAPTER 18 NOTES
PROPERTY OF: BIOLOGY – UNIT 3 – CHAPTER 18 NOTES

... blurred lines between species, genera, or families taxons change as soon as new evidence is discovered species are now grouped by close evolutionary relationships, not necessarily by similar physical features EX: homologous structures (similar body parts that evolved from a common ancestor) vs. anal ...
siop lesson plan for
siop lesson plan for

... SLIDE: Organelles: In cell biology, an organelle /ɔrɡəˈnɛl/ is a specialized subunit within a cell that has a specific function, and it is usually separately enclosed within its own lipid bilayer. The name organelle comes from the idea that these structures are to cells what an organis to the body ...
Evaluation of Reversine as an anticancer drug in oral cancer
Evaluation of Reversine as an anticancer drug in oral cancer

... Reversine is a synthetic purine-like small molecular which is first identified as a de-differentiation agent in murine myoblast cell lines. Moreover, it has been demonstrated as an inhibitor of Aurora kinase and which can prohibit the survival of leukemia cells. Because Aurora kinase has also been r ...
The Cell Cycle and Cell Division
The Cell Cycle and Cell Division

... actively bind proteins for the segregation, using ATP. An actin-like protein provides a filament along which ori and other proteins move. ...
Mitosis and Meiosis
Mitosis and Meiosis

... each with half the number of stage of mitosis. opposite ends of the cell. chromosomes as the original. ...
The New and Improved Taxonomy Chart
The New and Improved Taxonomy Chart

... Reproduction: Asexually (binary fission or multiple fission), sexually (conjugation) ...
Edexcel AS/A level Biology
Edexcel AS/A level Biology

... chromosomes breaking during mitosis. A single mutation changing a proto-oncogene to an oncogene can cause cancer. Proto-oncogenes code for proteins which stimulate the cell cycle. Oncogenes produce uncontrolled amounts of stimulating proteins. Tumour-suppressor genes code for the production of chemi ...
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(a) Gram-positive bacteria

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What is a cell?

... Most plants and animals are multicellular. The human body is made up of around 200 different types of cell, all working together. Most cells are specialized, meaning that each type of cell has a specific structure and function. All cells with a nucleus contain the same genes, but different cells act ...
reproducing - Southgate Schools
reproducing - Southgate Schools

... ___________________to each other and to the original parent cell. • Mitosis allows an organism's body to _______________________________cells. • In asexual reproduction, a new organism is produced by mitosis of the cell or cells of the parent organism. Meiosis Summary ...
The Cellular Level of Organization
The Cellular Level of Organization

... Such substances may move through the membrane with the aid of transporter proteins, moving down their concentration gradient to a region of lower concentration. This process is called facilitated diffusion. It can be much ...
RAS (overview) Midwest 2013
RAS (overview) Midwest 2013

... The Ras mitogen-activated protein kinase (MAPK) cascade, including Raf, Mek and Erk, is a ubiquitous signaling module that couples receptor-mediated events at the cell surface to cytoplasmic and nuclear effectors. The Ras MAPK cascade is perhaps best known for its crucial role in mediating the trans ...
Tool E - Workshops+SJCOE Workshop Management
Tool E - Workshops+SJCOE Workshop Management

... Students use the model to describe a causal account for the phenomenon, including how different parts of a cell contribute to how the cell functions as a whole, both separately and together with other structures. Students include how components, separately and together, contribute to: i. Maintaining ...
Eukaryotic Cell Structure: Organelles in Animal
Eukaryotic Cell Structure: Organelles in Animal

... Eukaryotic Cell Structure: Organelles in Animal & Plant Cells Why are organelles important and how are plants and animals different? ...
Case-It - South. Blot.
Case-It - South. Blot.

... is considered a recessive trait, since both chromosomes have to carry the mutation in order for the full blown disease symptoms to appear. The sickle cell mutation also eliminates a restriction enzyme site - the recognition site for the enzyme MstII. To detect the sickle cell mutation, a patient’s D ...
Bellringer
Bellringer

... point in their lives. DNA is the genetic material that carries instructions for making new cells. • In eukaryotic cells, including plants and animals, the DNA is found within the nucleus of the cell. • The nucleus is an organelle specialized to hold the DNA. The nucleus plays a role in growth, metab ...
بسم الله الرحمن الرحيم
بسم الله الرحمن الرحيم

... *Fimbriae and pili are short, hair-like structures on the surfaces of procaryotic cells. *they are composed of protein. *Fimbriae are shorter and stiffer than flagella, and slightly smaller in diameter. *fimbriae have nothing to do with bacterial movement. *Fimbriae are very common in Gram-negative ...
BSc MOLECULAR BIOLOGY AND BIOCHEMISTRY (C701)
BSc MOLECULAR BIOLOGY AND BIOCHEMISTRY (C701)

... During the Third Year candidates must undertake not less than 40 weeks in industry or in a research institution engaged in placement work approved by the Board of Studies in Biological Sciences. During the placement student progress will be monitored through two interviews with the University superv ...
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Specialized Cell Structures

... – Converts sugars into ATP (energy) for the cell ...
Chapter 9 - www.jgibbs-vvc
Chapter 9 - www.jgibbs-vvc

... motor end plate and contains receptors for acetylcholine. The space between the motor end plate and the motor neuron is called the synaptic cleft. 32. a lipid “bubble” formed by a phospholipid bilayer. Acetylcholine 33. cleft 34. exocytosis 35. an enzyme called acetylcholinesterase. As long as it is ...
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File - Science by Shaw

... ocean over and above how the currents take them. The force of the water combined with the force of the fish in motion enable it to move. ...
Comparative Analysis of Transcriptional Regulation in Eukaryotic
Comparative Analysis of Transcriptional Regulation in Eukaryotic

... 2.2 Analysis of orthologous relationships between cell cycle regulated genes We observed orthologous relationships between the cell cycle regulated genes in each species using KEGG/OC(OrthologCluster) [5,6], which assigns an orthologous identifier to each gene with sequence similarities stored in KE ...
Plant vs. Animal Cells Date
Plant vs. Animal Cells Date

... INM ...
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Cytokinesis



Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.
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