BIOLOGICAL SCIENCES - Plant Biology PP2A
... meristem (Fig. S2). To confirm this, we analyzed seedlings expressing a pPP2A-3::n3xGFP fusion and assessed expression in the root tip. In 5 independent transformants we indeed observed pPP2A-3::n3xGFP expression in the root tip, but PP2A-3 was more broadly expressed than ACR4 (5) (Fig. 2 A and Fig ...
... meristem (Fig. S2). To confirm this, we analyzed seedlings expressing a pPP2A-3::n3xGFP fusion and assessed expression in the root tip. In 5 independent transformants we indeed observed pPP2A-3::n3xGFP expression in the root tip, but PP2A-3 was more broadly expressed than ACR4 (5) (Fig. 2 A and Fig ...
Studies on Cell Adhesion and Recognition I. Extent and Specificity
... The adhesion surfaces were prepared by adsorbing different proteins on microtiter wells (see above). Unless otherwise indicated, fibronectin and different lectins were adsorbed at 10 pg/ml. The enzyme concentrations were 0.2 U/ml of Clostridium perfringens sialidase and 0.9 U/ml of 6-galactosidase ( ...
... The adhesion surfaces were prepared by adsorbing different proteins on microtiter wells (see above). Unless otherwise indicated, fibronectin and different lectins were adsorbed at 10 pg/ml. The enzyme concentrations were 0.2 U/ml of Clostridium perfringens sialidase and 0.9 U/ml of 6-galactosidase ( ...
PDF - Walter Lab
... by FM4-64 and Hxt2–GFP fluorescence. Thus, the aberrant plasma membrane invaginations seen in the electron micrographs are likely to be sites of clustered eisosome remnants. These results suggest that eisosomes are required for the proper spatial distribution of endocytic events. In the absence of P ...
... by FM4-64 and Hxt2–GFP fluorescence. Thus, the aberrant plasma membrane invaginations seen in the electron micrographs are likely to be sites of clustered eisosome remnants. These results suggest that eisosomes are required for the proper spatial distribution of endocytic events. In the absence of P ...
The learners demonstrate an understanding of: The learners shall
... bacterial cell using recyclable materials 2. construct a cell membrane model from indigenous or recyclable materials ...
... bacterial cell using recyclable materials 2. construct a cell membrane model from indigenous or recyclable materials ...
Myocardial tissue engineering with cells derived from human
... In vivo assessments of the hCMPs were performed in a murine model of myocardial infarction (MI). MI was surgically induced as described previously,20 then, animals in the MI+hCMP group were treated with two hCMPs, animals in the MI+Scaffold group were treated with two patches of scaffold material wi ...
... In vivo assessments of the hCMPs were performed in a murine model of myocardial infarction (MI). MI was surgically induced as described previously,20 then, animals in the MI+hCMP group were treated with two hCMPs, animals in the MI+Scaffold group were treated with two patches of scaffold material wi ...
LL37 peptide@silver nanoparticles: combining control
... Silver source was changed every 48 h in fresh cell culture media. Error bars correspond to SD from six different samples obtained from triplicate experiments. T‐Student two tail test, *p<0.1, **p<0.01, ***p<0.001. (B) Percentage of cells stained with AM‐Calcein (green bars) or ethidium bromide hom ...
... Silver source was changed every 48 h in fresh cell culture media. Error bars correspond to SD from six different samples obtained from triplicate experiments. T‐Student two tail test, *p<0.1, **p<0.01, ***p<0.001. (B) Percentage of cells stained with AM‐Calcein (green bars) or ethidium bromide hom ...
Optical tweezers - Niels Bohr Institutet
... optical trap on the trapped cell. • One key issue is to choose a wavelength which is not absorbed by water (would create heating) and not absorbed by biological specimen either. • Infra-red lasers fulfill these criteria. ...
... optical trap on the trapped cell. • One key issue is to choose a wavelength which is not absorbed by water (would create heating) and not absorbed by biological specimen either. • Infra-red lasers fulfill these criteria. ...
How do we best estimate phytoplankton biomass?
... 5.2.1 Chlorophyll concentration in the water column 5.2.2. Water transparency related to increase in suspended algae, where relevant 5.2.4 Species shift in floristic composition such as diatom to flagellate ratio bloom events of nuisance/toxic algal blooms ...
... 5.2.1 Chlorophyll concentration in the water column 5.2.2. Water transparency related to increase in suspended algae, where relevant 5.2.4 Species shift in floristic composition such as diatom to flagellate ratio bloom events of nuisance/toxic algal blooms ...
PDF
... 13-day chick embryos. When it is applied, in the form of an insoluble pellet, to amphibian blastula ectoderm it causes the formation of a range of mesodermal cell types, including notochord, muscle, kidney and blood (Asashima & Grunz, 1983; Grunz, 1983). The relevance of this chick-derived factor to ...
... 13-day chick embryos. When it is applied, in the form of an insoluble pellet, to amphibian blastula ectoderm it causes the formation of a range of mesodermal cell types, including notochord, muscle, kidney and blood (Asashima & Grunz, 1983; Grunz, 1983). The relevance of this chick-derived factor to ...
Epithelial repair is a two-stage process driven first by dying cells and
... Fig. 1. Removal of dying cells is a two-stage process consisting of apical contraction followed by extrusion. (A) Confocal time-lapse images (individual Z planes) of UV-treated lifeact–GFP MDCK cells. Timings start 1 h 20 min after UV treatment; xz and xy planes are indicated by corresponding lines ...
... Fig. 1. Removal of dying cells is a two-stage process consisting of apical contraction followed by extrusion. (A) Confocal time-lapse images (individual Z planes) of UV-treated lifeact–GFP MDCK cells. Timings start 1 h 20 min after UV treatment; xz and xy planes are indicated by corresponding lines ...
C. Dermis
... The components of ground substance are principally proteoglycans and glycoproteins whose molecular weight is 150,000 to 250,000 and whose sugar content is 2% to 15%. The molecules stabilize the fibers to give flexibility to the skin. Fibronectin, one kind of glycoprotein, contains a domain that conn ...
... The components of ground substance are principally proteoglycans and glycoproteins whose molecular weight is 150,000 to 250,000 and whose sugar content is 2% to 15%. The molecules stabilize the fibers to give flexibility to the skin. Fibronectin, one kind of glycoprotein, contains a domain that conn ...
cell-substrate contacts in cultured chick embryonic cells
... These changes in cell contacts appeared more quickly in some tissues than in others. After 24 h, explants of hypoblast and definitive endoblast could easily be distinguished but by 7 days they were very similar. In the absence of serum, specialized cell contacts developed more quickly; in higher con ...
... These changes in cell contacts appeared more quickly in some tissues than in others. After 24 h, explants of hypoblast and definitive endoblast could easily be distinguished but by 7 days they were very similar. In the absence of serum, specialized cell contacts developed more quickly; in higher con ...
Formative pluripotency: the executive phase in a developmental
... potential to generate all cell lineages of the mature organism. Recently, pluripotency has been described in two forms: naïve and primed (Hackett and Surani, 2014; Nichols and Smith, 2009). These terms refer to pre- and post-implantation populations in the embryo and their associated in vitro stem c ...
... potential to generate all cell lineages of the mature organism. Recently, pluripotency has been described in two forms: naïve and primed (Hackett and Surani, 2014; Nichols and Smith, 2009). These terms refer to pre- and post-implantation populations in the embryo and their associated in vitro stem c ...
Potential effect of spliceosome inhibition in small cell lung
... Competing interests: The authors have declared that no competing interests exist. ...
... Competing interests: The authors have declared that no competing interests exist. ...
RNA Processing Bodies, Peroxisomes, Golgi
... by the cytoskeleton. In plants, actin filaments sustain the long-distance transport of many types of organelles, and microtubules typically fine-tune the motile behavior. In shoot epidermal cells of Arabidopsis thaliana seedlings, we show here that a type of RNA granule, the RNA processing body (P-b ...
... by the cytoskeleton. In plants, actin filaments sustain the long-distance transport of many types of organelles, and microtubules typically fine-tune the motile behavior. In shoot epidermal cells of Arabidopsis thaliana seedlings, we show here that a type of RNA granule, the RNA processing body (P-b ...
Physalis angulata induces in vitro differentiation of murine bone
... Bioimagem, Rio de Janeiro, Brazil Full list of author information is available at the end of the article ...
... Bioimagem, Rio de Janeiro, Brazil Full list of author information is available at the end of the article ...
Exine dehiscing induces rape microspore polarity
... 2001; Fischer et al., 2004). The role of cell polarity in multicellular system development and differentiation, especially during embryo development, has attracted increasing attention (Jurgens et al., 1997; Kropf, 1999; Scheres and Benfey, 1999; Grebe et al., 2002; Ekici and Dane, 2004). Analyses i ...
... 2001; Fischer et al., 2004). The role of cell polarity in multicellular system development and differentiation, especially during embryo development, has attracted increasing attention (Jurgens et al., 1997; Kropf, 1999; Scheres and Benfey, 1999; Grebe et al., 2002; Ekici and Dane, 2004). Analyses i ...
Photosynthesis Worksheet - d
... 9 A by-product of photosynthesis. (6) 10 Number of molecules of oxygen produced along with one molecule of sugar. (3) ...
... 9 A by-product of photosynthesis. (6) 10 Number of molecules of oxygen produced along with one molecule of sugar. (3) ...
Seminars in Cancer Biology Invasion emerges from cancer cell
... be represented altogether, in order to capture their effects fully. These views have been immensely helpful to unearth enormous amounts of information. However, in our modeling efforts we found it useful to sacrifice detail in order to distill essential elements of the mE. For now, we are focusing on ...
... be represented altogether, in order to capture their effects fully. These views have been immensely helpful to unearth enormous amounts of information. However, in our modeling efforts we found it useful to sacrifice detail in order to distill essential elements of the mE. For now, we are focusing on ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.