Full_ppt_ch21
... – A product of a reaction acts as a negative regulator – An end product binds with the first enzyme in a sequence when sufficient product is present ...
... – A product of a reaction acts as a negative regulator – An end product binds with the first enzyme in a sequence when sufficient product is present ...
Citric Acid Cycle Regulation
... If no Oxygen around, cant enter Citric Acid Cycle (CAC). Can only do glycolysis. Each round of glycolysis produces a net gain of 2 ATPs. Better than nothing so use glycolysis. But supply of NAD+ is limited in cytoplasm so must regenerate it to allow glycolysis to continue! Step 5 of glycolysis conve ...
... If no Oxygen around, cant enter Citric Acid Cycle (CAC). Can only do glycolysis. Each round of glycolysis produces a net gain of 2 ATPs. Better than nothing so use glycolysis. But supply of NAD+ is limited in cytoplasm so must regenerate it to allow glycolysis to continue! Step 5 of glycolysis conve ...
Bio II Elodea Lab: Photosynthesis and Cellular
... The ETC does not make ATP directly. Instead, it generates a ___________________________which stores potential energy that can be used to phosphorylate ADP to ATP later. Some electron carriers accept and release _____________along with the electrons. While the chain is operating, the pH in the membra ...
... The ETC does not make ATP directly. Instead, it generates a ___________________________which stores potential energy that can be used to phosphorylate ADP to ATP later. Some electron carriers accept and release _____________along with the electrons. While the chain is operating, the pH in the membra ...
Page 20-1 CHAPTER 20: Enzymes 20.2
... energy for normal reaction rate • Above optimum temperature, irreversible changes in 3-D structure (3° structure destroyed) ...
... energy for normal reaction rate • Above optimum temperature, irreversible changes in 3-D structure (3° structure destroyed) ...
Hexokinase
... Figure 18.2 Pyruvate produced in glycolysis can be utilized by cells in several ways. In animals, pyruvate is normally converted to acetylcoenzyme A, which is then oxidized in the TCA cycle to produce CO2. When oxygen is limited, pyruvate can be converted to lactate. Alcoholic fermentation in yeast ...
... Figure 18.2 Pyruvate produced in glycolysis can be utilized by cells in several ways. In animals, pyruvate is normally converted to acetylcoenzyme A, which is then oxidized in the TCA cycle to produce CO2. When oxygen is limited, pyruvate can be converted to lactate. Alcoholic fermentation in yeast ...
Lipid Metabolism Catabolism Overview
... acid phenyl derivatives and then analyzed their urine for the resulting metabolites. What metabolite was produced when dogs were fed ...
... acid phenyl derivatives and then analyzed their urine for the resulting metabolites. What metabolite was produced when dogs were fed ...
TYK2 (JTK1), Active TYK2 (JTK1), Active
... incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spotting 20 µl of the reaction mixture onto individual pre-cut strips of phosphocellulose P81 paper. Step 7. Air dry the pre-cut P81 strip and sequentially wash in a ...
... incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spotting 20 µl of the reaction mixture onto individual pre-cut strips of phosphocellulose P81 paper. Step 7. Air dry the pre-cut P81 strip and sequentially wash in a ...
Document
... Binding site Area that holds substrate in proper place. Enzymes use weak, non-covalent interactions to hold the substrate in place based on R groups of ...
... Binding site Area that holds substrate in proper place. Enzymes use weak, non-covalent interactions to hold the substrate in place based on R groups of ...
Enzyme Specificity and Selectivity
... hydrophobic residues such as tryptophan, phenylalanine and tyrosine, while elastase hydrolyses peptides at small aliphatic residues such as alanine and valine. Thus, the products of enzyme-catalysed peptide hydrolysis will vary greatly depending upon the enzyme utilized. The serine protease reaction ...
... hydrophobic residues such as tryptophan, phenylalanine and tyrosine, while elastase hydrolyses peptides at small aliphatic residues such as alanine and valine. Thus, the products of enzyme-catalysed peptide hydrolysis will vary greatly depending upon the enzyme utilized. The serine protease reaction ...
Chapter 6
... At this point the original 6C sugar has been converted to 2 moles of the 3C aldehyde, G3P. This conversion has consumed 2 moles of ATP and has thus been an energy drain on the cell. The glyceraldehyde-3-P is now oxidized to the corresponding acid. This reaction is one of the best understood examples ...
... At this point the original 6C sugar has been converted to 2 moles of the 3C aldehyde, G3P. This conversion has consumed 2 moles of ATP and has thus been an energy drain on the cell. The glyceraldehyde-3-P is now oxidized to the corresponding acid. This reaction is one of the best understood examples ...
Fatty acid catabolism leture2-3
... Fatty acid (FA) activation before oxidation All the enzymes involved in oxidation of FA are present in mitochondria. The free FA obtained from blood cannot enter mitochondia. In the first step, FA are converted to fatty acyl CoA on the outer mitochodrial membrane by an ezyme called Fatty acyl CoA sy ...
... Fatty acid (FA) activation before oxidation All the enzymes involved in oxidation of FA are present in mitochondria. The free FA obtained from blood cannot enter mitochondia. In the first step, FA are converted to fatty acyl CoA on the outer mitochodrial membrane by an ezyme called Fatty acyl CoA sy ...
ภาพนิ่ง 1
... • Experimentally, Km is a useful parameter for characterizing the number and/or types of substrates that a particular enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate- ...
... • Experimentally, Km is a useful parameter for characterizing the number and/or types of substrates that a particular enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate- ...
Product Information Sheet - Sigma
... neutral aqueous solutions. This product may contain up to 40 ppm vanadium. Sigma offers this product for those customers who have historically used it or for those who do not require a low vanadium preparation. PHYSICAL DESCRIPTION: Formula - C10H14N5O13P3Na2 Formula Weight - 551.1 Apparent molecula ...
... neutral aqueous solutions. This product may contain up to 40 ppm vanadium. Sigma offers this product for those customers who have historically used it or for those who do not require a low vanadium preparation. PHYSICAL DESCRIPTION: Formula - C10H14N5O13P3Na2 Formula Weight - 551.1 Apparent molecula ...
Partial purification of fatty acid synthetase from Streptomyces
... Thus the synthetase migrated as a single species during these Filamentous bacteria of the genus Streptomyces are extremely procedures (with a consistent recovery of over 80%), and its versatile in making antibiotics, many of which are phenolic activity presumably resides in multifunctional polypepti ...
... Thus the synthetase migrated as a single species during these Filamentous bacteria of the genus Streptomyces are extremely procedures (with a consistent recovery of over 80%), and its versatile in making antibiotics, many of which are phenolic activity presumably resides in multifunctional polypepti ...
Peroxisomal oxidation of fatty acids
... before oxidation All the enzymes involved in oxidation of FA are present in mitochondria. The free FA obtained from blood cannot enter mitochondia. In the first step, FA are converted to fatty acyl CoA on the outer mitochodrial membrane by an ezyme called Fatty acyl CoA synthase (also called thiokin ...
... before oxidation All the enzymes involved in oxidation of FA are present in mitochondria. The free FA obtained from blood cannot enter mitochondia. In the first step, FA are converted to fatty acyl CoA on the outer mitochodrial membrane by an ezyme called Fatty acyl CoA synthase (also called thiokin ...
supplementary material
... enzyme. For lactate measurement, the reaction mixture contained 1% hydrazine, 4 mM NAD+ and LDH as auxiliary enzyme. The buffer was 0.2 M glycine of pH 9.6 3. Western Blot. Proteins were separated by SDS/PAGE (12% gel) and electrotransferred to PVDF transfer membrane for 1-3 hour at 140 mA. The filt ...
... enzyme. For lactate measurement, the reaction mixture contained 1% hydrazine, 4 mM NAD+ and LDH as auxiliary enzyme. The buffer was 0.2 M glycine of pH 9.6 3. Western Blot. Proteins were separated by SDS/PAGE (12% gel) and electrotransferred to PVDF transfer membrane for 1-3 hour at 140 mA. The filt ...
Chapter 10 Photosynthesis Part 2
... –Occurs in the stroma –Forms sugar from carbon dioxide, using ATP for energy and NADPH for reducing power ...
... –Occurs in the stroma –Forms sugar from carbon dioxide, using ATP for energy and NADPH for reducing power ...
Lecture 12 - Biocatalysis
... • Products have a different shape from the substrate • Once formed, they are released from the active site • Leaving it free to become attached to another substrate ...
... • Products have a different shape from the substrate • Once formed, they are released from the active site • Leaving it free to become attached to another substrate ...
PAGES 1-41 INCL. 1. Overview (a) discovery of enzymes (b
... v. presence of interfering substances - at the start of a reaction, amount of substrate begins to decline rate of decline is typically non-linear - later in the reaction, substrate concentration decreases at a slower rate. - same is true for formation of product. to measure reaction rates, an en ...
... v. presence of interfering substances - at the start of a reaction, amount of substrate begins to decline rate of decline is typically non-linear - later in the reaction, substrate concentration decreases at a slower rate. - same is true for formation of product. to measure reaction rates, an en ...
Supporting material
... program KaleidaGraph. logY = log(C/(1 + [H]/Ka + Kb/[H])) (3) where Y is kcat or kcat/Km, [H] is the hydrogen ion concentration, C is the kcat or kcat/Km value where it does not change with pH, Ka is the acid dissociation constant and Kb is the base dissociation constant. The stability of the A. eva ...
... program KaleidaGraph. logY = log(C/(1 + [H]/Ka + Kb/[H])) (3) where Y is kcat or kcat/Km, [H] is the hydrogen ion concentration, C is the kcat or kcat/Km value where it does not change with pH, Ka is the acid dissociation constant and Kb is the base dissociation constant. The stability of the A. eva ...
Glycolysis Citric Acid Cycle Krebs Cycle Oxidative
... no ATP produced; makes NAD+ needed for glycolysis to continue Part of the Cori Cycle at right ...
... no ATP produced; makes NAD+ needed for glycolysis to continue Part of the Cori Cycle at right ...
Chapter 8 Enzymes: Basic Concepts and Kinetics
... the first step in enzymatic catalysis What is the evidence for the existence of an enzymesubstrate complex? ...
... the first step in enzymatic catalysis What is the evidence for the existence of an enzymesubstrate complex? ...
Luciferase
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The name is derived from Lucifer, the root of which means 'light-bearer' (lucem ferre). One example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. ""Firefly luciferase"" as a laboratory reagent often refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.